CULTURE ENGINEERING DIFFERENTIATION CHARACTERIZATION. Stem Cell Research Solutions Promotional offers

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1 CULTURE ENGINEERING DIFFERENTIATION CHARACTERIZATION Stem Cell Research Solutions Promotional offers

2 Stem Cell research products For more than a decade, we have provided you with key tools to address challenges in stem cell research. Now we ve harnessed the power of these innovations to offer a total system covering the entire research spectrum. With our complete portfolio of stem cell products and services, you can benefit from the continuity, efficiency, and precision that drives discovery. With Gibco stem cell culture products, a broad Invitrogen portfolio of stem cell differentiation and engineering products, and a wide array of stem cell analysis tools that include Molecular Probes labeling and detection reagents, we have the products to support you at every step of your research. SPECIAL OFFERS* OFFER 1 OFFER 2 Order 2 qualifying products and receive a 10% discount. Order 3 or more qualifying products and receive a 20% discount. Quote promo code RLNS02 when ordering. Offers expire December 3, *Terms and conditions apply. See back page for complete details. 2 Life Technologies Stem Cell Research Solutions

3 Pluripotent Stem Cells (PSCs) Medium Cells 1 st operation 2 nd operation Rotate the dish 90 and roll again KnockOut Serum Replacement Gold for -ESC and ipsc cultures KnockOut Serum Replacement (KnockOut SR) is a defined, serumfree formulation used to support the growth of undifferentiated ES and ips in culture. ES and ips cells grown in KnockOut SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut SR is further enhanced when used in conjunction with KnockOut DMEM, a special basal medium designed specifically for ES and ips cell culture. Feeder free, Serum free Induced Pluripotent Stem Cell Culture Essential 8 Medium, originally developed in the laboratory of James Thomson, contains the 8 essential components needed for pluripotent stem cell culture. Essential 8 Medium enables streamlined expansion of PSCs. Essential 6 Medium is the same formulation as Essential 8 Medium, but does not contain TGF-ϐ or bfgf and enables reprogramming and embryoid body formation/differentiation. StemPro EZPassage Disposable Stem Cell Passaging Tool The StemPro EZPassage Disposable Stem Cell Passaging Tool is a novel human embryonic stem cell passaging device that makes manual passaging of stem cell colonies more rapid and reproducible. Essential 8 Medium 500 ml A Vitronectin (VTN-N) Recombinant Human Protein, Truncated 1 ml A14700 KnockOut Serum Replacement 500 ml StemPro hesc SFM 1 kit A KnockOut D-MEM CTS 500 ml A CTS KnockOut DMEM/F ml A KnockOut D-MEM 500 ml KnockOut DMEM/F ml KnockOut SR XenoFree CTS 100 ml KnockOut SR XenoFree CTS 500 ml Essential 6 Medium 500 ml A StemPro EZPassage Disposable Stem Cell Passaging Tool 10 units Geltrex LDEV-Free hesc-qualified Reduced Growth Factor Basement Membrane Matrix 1 ml A Geltrex hesc-qualified Ready-To-Use 50 ml A Learn more about our stem cell products and resources at lifetechnologies.com/stemcells Life Technologies Stem Cell Research Solutions 3

4 ipsc Reprogramming Tools CytoTune ips 2.0 Sendai Reprogramming Kit The CytoTune -ips 2.0 kit utilizes a non-integrating Sendai virus particles to deliver the Yamanaka factors and is extremely easy to use, requiring only one overnight incubation compared to the multiple days of transductions required for mrna reprogramming. Sendai reprogramming vectors do not integrate into chromosomes of the target cells and therefore do not potentially disrupt important genes. They are capable of transducing a wide range of cell types such as BJ fibroblasts, PBMCs, and CD34+ blood cells. Epi5 Episomal ipsc Reprogramming Kit The Epi5 Episomal ipsc Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for ips Cell Research and Application (CiRA), Kyoto University. This system produces transgene-free, virusfree human induced pluripotent stem cells (ipscs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed. Lipofectamine 3000 reagent with the Epi5 Episomal ipsc Reprogramming Kit enables highly efficient reprogramming of somatic cells without the need for electroporation. CytoTune 2.0 -ips Sendai Reprogramming Kit (1 Pack) 1 pack A16517 CytoTune 2.0-iPS Sendai Reprogramming Kit (3 Pack) 3 pack A16518 Epi5 Episomal ipsc Reprogramming Kit 1 kit A15960 Lipofectamine 3000 Transfection Reagent 0.75 ml L Lipofectamine 3000 Transfection Reagent 1.5 ml L Lipofectamine 3000 Transfection Reagent 5x1.5 ml L Lipofectamine 3000 Transfection Reagent 15 ml L Life Technologies Stem Cell Research Solutions

5 Mesenchymal Stem Cells (MSCs) Gibco media include the broadest selection of specialty products optimized for mesenchymal stem cell populations, many of which are free of animal-derived components. These media are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements. StemPro BM Mesenchymal Stem Cells 1 x 10 6 cells A15652 StemPro Human Adipose-Derived Stem Cell Kit 1 kit R StemPro Human Adipose-Derived Stem Cells 1 vial R MesenPRO RS Medium (NZ) 1 kit E CTS StemPro MSC SFM 1 kit A StemPro MSC SFM XenoFree 1 kit A StemPro Adipogenesis Differentiation Kit 1 kit A StemPro Chondrogenesis Differentiation Kit 1 kit A StemPro Osteogenesis Differentiation Kit 1 kit A Neuronal Stem Cells (NSCs) StemPro NSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human neural stem cells (NSCs). It enables superior human NSC expansion of both adherent and spheroid cultures derived from human embryonic stem cells or fetal tissue compared to existing commercial products. StemPro NSC SFM also maintains the multipotential differentiation capability into astrocytes, oligodendrocytes and neurons. Neurobasal Medium (1X) Minus Phenol Red 500 ml Neurobasal Medium (1X), Liquid 500 ml Neurobasal -A Medium (1X) Minus Phenol Red 500 ml Neurobasal -A Medium (1X), Liquid 500 ml StemPro NSC SFM 1 kit A B-27 Serum-Free Supplement (50X), Liquid 10 ml B-27 Supplement Minus AO (50X), Liquid 10 ml B-27 Supplement Minus Vitamin A (50X), Liquid 10 ml G-5 Supplement (100X), Liquid 1 ml Hematopoietic Stem Cells (HSCs) Life Technologies provides a comprehensive set of tools for isolation, expansion, differentiation, and characterization of HSCs and their derivatives. CD34 is a common marker for identifying human HSCs, and Life Technologies offers a serum-free medium for CD34+ cells, as well as methods to isolate CD34+ cells from blood or bone marrow. StemPro CD34+ Cell Kit 0.5 x 10 6 cells ml A14059 StemPro -34 SFM (1X), Liquid 1 kit Learn more about our stem cell products and resources at lifetechnologies.com/stemcells Life Technologies Stem Cell Research Solutions 5

6 Coating reagents Fibronectin Human, Plasma 5 mg Fibronectin, Bovine Plasma 1 mg CELLstart CTS 2 ml A Natural Mouse Laminin 1 mg Dissociation reagents Dispase 5 g StemPro Accutase Cell Dissociation Reagent 100 ml A UltraPure 0.5M EDTA, ph 8.0 4x100 ml TrypLE Select CTS 100 ml A Characterization Stem cell research requires cellular and molecular tools to confirm pluripotency or to help determine the utility of cells in downstream experiments. Whether analyzing proliferation, protein levels, gene expression, or epigenetic profiles, Life Technologies has the right instruments, products, and services for your research. Alkaline Phosphatase Live Stain 50 µl A14353 AlexaFluor 488 CD44 50 tests A25528 AlexaFluor 488 Tra tests A25618 AlexaFluor 555 Tra tests A24879 AlexaFluor 594 Tra tests A24882 SSEA4 Mouse Anti-Human mab (clone ebiomc (MC )), Alexa Fluor tests A14810 SSEA4 Mouse Anti-Human mab (clone MC813-70), Alexa Fluor 647 Conjugate 0.5 ml SSEA421 SSEA4 Mouse Anti-Human mab (clone ebiomc (MC )), PE Conjugate 50 µg A14766 CD44 Rat Anti-Human/Mouse mab (clone IM7) Fluorescein (FITC) Conjugate 50 µg A16193 Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit 40 tests A24881 Pluripotent Stem Cell Immunocytochemistry Kit (Sox2, Tra-1-60) 40 tests A25525 Pluripotent Stem Cell Immunocytochemistry Kit (Oct4, SSEA4) 40 tests A25526 Human Neural Stem Cell Immunocytochemistry Kit 20 tests A24354 Human Cardiomyocyte Immunocytochemistry Kit 50 tests A25973 TaqMan hpsc Scorecard Panel, 96-well fast (1 kit) 2 x 96 well plates A15871 TaqMan hpsc Scorecard Panel, 96-well fast (1 panel) 2 x 96 well plates A15876 TaqMan hpsc Scorecard Panel, 384-well (1 kit) 1 x 384 well plates A15872 TaqMan hpsc Scorecard Panel, 384-well (1 panel) 1 x 384 well plates A Life Technologies Stem Cell Research Solutions

7 Achieve better biology in your stem cell research Lipofectamine 3000 Reagent Generation and transfection of induced pluripotent stem cells (ipscs). Learn more at lifetechnologies.com/3000 CULTURE ENGINEERING DIFFERENTIATION CHARACTERIZATION Lipofectamine 3000 reagent with the Epi5 Episomal ipsc Reprogramming Kit enables highly efficient reprogramming of somatic cells without the need for electroporation. Lipofectamine 3000 transfection reagent also improves the cleavage efficiency with CRISPR vectors on pluripotent stem cells, yielding a 2-fold increase in the efficiency of genetic modifications. Adult cells Indel in genomic DNA CULTURE ENGINEERING DIFFERENTIATION CHARACTERIZATION CULTURE ENGINEERING DIFFERENTIATION CHARACTERIZATION Lipofectamine 3000 reagent can deliver DNA into ESCs and ipscs in culture. Lipofectamine 3000 reagent can deliver DNA into difficult-to-transfect differentiated cells in culture. Ectoderm ipscs Mesoderm Endoderm Reprogramming factors Differentiation Gibco media and supplements for PSC differentiation provide robust, physiologically relevant results with simple protocols. These innovative tools are designed to simplify and speed up complex protocols for differentiating PSCs in to different cells types so you can spend more time focusing on the outcomes of your research. Product Size Catalog Number Gibco PSC Neural Induction Medium PSC Cardiomyocyte Differentiation Kit (prototype) 1 kit 1 kit A A25042SA Learn more about our stem cell products and resources at lifetechnologies.com/stemcells Life Technologies Stem Cell Research Solutions 7

8 CytoTune -ips 2.0 Sendai Reprogramming Kit The highest reprogramming efficiency for ips cell generation Learn more at lifetechnologies.com/cytotune *Terms and conditions: This promotion is open only to customers in Australia, India, New Zealand and Singapore who purchase two or more qualifying products in a single purchase order during the promotional period. The list of qualifying products include all product catalog numbers listed throughout this brochure. Offers will apply to eligible orders received by Life Technologies no later than December 3, 2014 or until promotional supplies are depleted, whichever comes first. Discount applies to the list price in effect at the time the order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CytoTune is a trademark of DNAVEC Corporation. Essential 8 & Essential 6 are trademarks of Cellular Dynamics International, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. C IPAC 1014