Vision General de la Esterilización y Desinfectión antes y hoy General Overview of the Sterilization and Disinfection Once and Today

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1 Vision General de la Esterilización y Desinfectión antes y hoy General Overview of the Sterilization and Disinfection Once and Today Vlatka Turcic MD. MSc. WFHSS, Croatia

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4 BC

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7 BC

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9 Oliver Wendell Holmes Ignaz Semmelweiss

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11 History of disinfection The first disinfectant iodine (for cleaning wounds) Davies Chlorine water Le Ferne Introduced by Semmelweiss Lister 5% carbolic acid Antun Grosic iodine tincture The first disinfection of drinking water Pula, 19th century (great epidemic of typhoid fever) Samuel Davies Joseph Lister

12 Louis Pasteur ( ) The father of stereochemistry Phenol (wound disinfection) Alcoholic phermentation Pasteurization

13 Robert Koch ( ) Antrax Cholera Tuberculosis Koch s bacillus Malaria

14 Partisan barrel Boer wars

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16 Disinfection procedures Mechanical Physical Chemical Washing and cleaning Filtering Ultrasonic cleaning Heat UV radiation Chemical disinfection procedures

17 If by definition the disinfection procedures are said to destroy, inhibit or remove microorganisms, then washing and cleaning are the least aggressive and highly efficient procedures to remove microorganisms

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19 The resistance of microorganisms to disinfectants Mycoplasma Enveloped viruses Majority of the gram - positive bacteria Majority of the gram - negative bacteria Fungi and their spores Certain gram - positive bacteria Certain gram - negative bacteria Viruses without lipid envelopes Acid-resistant bacteria Bacterial spores Very sensitive Very resistant

20 The final efect of chemical disinfectants Biocidal - irreversible damage to the bacterial cells Biostatic reversible damages to bacterial cells the

21 Since the sterilization is a process destroying all types and all forms of microorganisms a way had to be discovered to achieve that goal

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24 Dry heat sterilizer

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27 Papin s steam digester 1679.

28 Louis Pasteur ( ) Proved that damp heat is more effective than dry heat

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31 Lister s sterilizer for medications

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33 Schimmelbusch s sterilizer

34 Chamberland s sterilizer

35 1934.

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38 Formaldehyde sterilizer

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40 Sterilizing by boiling

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43 Sterilization Precisely defined procedure guaranteeing the production of sterile goods Absolute notion (no compromises) Constitution, the organization of work and the infrastructure that provides all the necessary elements for carrying out the procedure (securing conditions to attain the target quality)

44 Sterilization In the narrow sense of the word effective sterilization is carried out in a quality, well-maintained and validated device sterilizer company.) (manufacturer or the service Everything else needed for the production of sterile goods is the system/infrastructure, which includes the organization of work, the conditions (space, sanitarytechnical preconditions, personnel, equipment selection, procurement of consumables) and the staff training (solely our responsibility)

45 In other words: Sterilization is not just a machine Sterilization is not only a method Sterilization is not only a service to meet the needs and wishes of the users Sterilization is a structure.

46 Sterilization is the heart of the hospital

47 the letter A in the alphabet of medicine

48 If sterilization stops working the hospital s heart will stop beating.

49 Sterilization methods Procedures for heat resistant materials Procedures for thermolabile materials

50 =?

51 Proving sterility Microbiological tests 30 samples for the: - aerobic bacteria - anaerobic bacteria - fungi - viruses

52 Sterilized Undergone the sterilization process The process of proving the success of the sterilization cycle is carried out exclusively by indirect methods of monitoring the proper operation of devices and supervising and controlling the procedures

53 Surveillance

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58 Organizing the space

59 The size of the space per zones according to the number of surgical procedures

60 The microclimatic conditions Laminar air flow must be provided with a minimum of 10 air changes per houror or 2 changes in spaces without ventilation Turbulent air flow causes air whirling and therefore must not be used in CSSD spaces The same applies to the split systems without conditioning. Relative air pressure must be negative in unclean and positive in clean zones Relative humidity of 30-60% is optimal, 40-50% Temperature for unclean zones is C and for clean zones it is C

61 The number and qualifications of the personnel, personnel training, equipment validation, procedure validation etc.

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Microorganisms are the agents of contamination, infection. Hence it becomes necessary to remove them from materials and areas.

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