DNA PURIFICATION KITS PLASMID DNA ISOLATION

Transcription

1 LIST -NEW v DNA PURIFICATION KITS PLASMID DNA ISOLATION Plasmid Mini ultrapure, high and medium copy number plasmid DNA isolation from ml of bacteria culture PURIFICATION TECHNOLOGY SM Plasmid Mini AX low and medium copy number and large plasmid DNA isolation from ml of bacteria culture AX Plasmid Midi AX ultrapure, plasmid DNA isolation from ml of bacteria culture DNA FRAGMENTS PURIFICATION Gel-Out DNA isolation from agarose AX SM Gel-Out AX DNA isolation from agarose (low melting point) AX Clean-Up DNA purification after enzymatic reactions SM Clean-Up AX DNA purification after enzymatic reactions AX Electro-Elution System DNA and RNA isolation from agarose and polyacrylamide AX ExTerminator nucleotide terminators removal kit for cycle sequencing reactions SM ExTerminator (96-well) 96-well format kit for nucleotide terminators removal from cycle sequencing reactions SM GENOMIC DNA PURIFICATION Genomic Mini genomic DNA purification from bacteria culture, cell culture or tissue. SM Genomic Midi AX genomic DNA purification from blood, bacteria culture, cell culture or tissue AX Genomic Maxi AX genomic DNA purification from blood, bacteria culture, cell culture or tissue AX Blood Mini specialized for genomic DNA purification from variety of blood samples Swab DNA isolation from swab samples Sherlock AX specialized for troublesome samples (excellent for forensic science) SM SM AX Genomic Mini AX BACTERIA DNA isolation from bacteria culture AX Genomic Mini AX BLOOD DNA isolation from fresh or frozen blood samples AX Genomic Mini AX FOOD DNA isolation from food samples AX Genomic Mini AX PLANT DNA isolation from plants AX Genomic Mini AX STOOL DNA isolation from stool samples AX Genomic Mini AX STREPTOMYCES DNA isolation from Streptomyces AX Genomic Mini AX TISSUE DNA isolation from tissue or cell cultures AX Genomic Mini AX YEAST DNA isolation from yeast AX Genomic Mini AX BACTERIA (96-well) 96-well format kit for DNA extraction from bacteria culture AX Genomic Mini AX BLOOD (96-well) 96-well format kit for DNA isolation from fresh or frozen blood samples AX Genomic Mini AX TISSUE (96-well) 96-well format kit for DNA extraction from tissue and cell tissue culture AX Genomic Mini AX BACTERIA Spin DNA isolation from bacteria culture AX S Genomic Mini AX BLOOD 0.3 ML Spin DNA isolation from ml fresh or frozen blood sample AX S Genomic Mini AX BLOOD 1.0 ML Spin DNA isolation from 1 ml fresh or frozen blood sample AX S Genomic Mini AX PLANT Spin DNA isolation from plants AX S Genomic Mini AX STOOL Spin DNA isolation from stool samples AX 0-100S Genomic Mini AX SOIL Spin DNA isolation from soil AX S Genomic Mini AX TISSUE Spin DNA isolation from tissue or cell cultures AX S /5

2 LIST Genomic Mini AX YEAST Spin DNA isolation from yeasts AX S Genomic Micro AX BLOOD Gravity DNA isolation from 0.1 ml fresh or frozen blood sample AX Genomic Micro AX SWAB Gravity DNA isolation from swab samples AX Genomic Micro AX SWAB Gravity Plus DNA isolation from swab samples including swab tools AX P Bead-Beat Micro AX Gravity universal kit for genomic DNA isolation with mechanic lysis AX PURIFICATION TECHNOLOGY SM - silica membranes AX - ion exchange membranes AX RNA PURIFICATION KITS Total RNA Mini total RNA purification from bacteria culture, cell culture, tissue or blood Total RNA Midi total RNA purification from bacteria culture, cell culture, tissue or blood Total RNA Maxi total RNA purification from bacteria culture, cell culture, tissue or blood Micro RNA low molecular weight RNA purification from bacteria culture, cell culture, tissue or blood VIRUS DNA / RNA PURIFICATION KITS Viral DNA / RNA Virus DNA/RNA isolation from blood samples Virus Mini AX TRANSFECT Ultra pure virus DNA isolation for downstream transfection T ENZYMES Run DNA polymerase (Taq DNA polymerase) U U 78 Walk DNA polymerase (Pwo DNA polymerase) U U 78 Marathon DNA polymerase (Taq and Pwo DNA polymerase mixture) U U 1 Protease mg 22 Proteinase K solution mg 22 Proteinase K lyophilized powder L 25 mg L 100 mg L 250 mg L 1000 mg 249 Lysostaphin U U 176 Lysozyme mg mg 1 RNAse (DNAse free) mg mg 202 DNAse (RNAse free) U U 92 T4 DNA Ligase U U 1 2/5

3 LIST SPECIALTY APPLICATION PRODUCTS Protein Precipitation Kit protein concentration kit for SDS PAGE reactions reactions 190 Anti-Inhibitor Kit kit for inhibitor removal from the DNA samples isolations isolations 201 REAGENTS FOR MOLECULAR BIOLOGY IPTG g g g 290 IPTG RTU 100 mm concentrated, ready-to-use IPTG solution S 15 ml (10 x 1.5 ml) 52 X-Gal g g 200 X-Gal RTU 20 mg / ml concentrated, ready-to-use X-Gal solution S 10 ml (10 x 1.0 ml) 92 Water DEPC ml (5x 1.5 ml) ml (5 x 5 ml) DTT (dithiothreitol) g g 218 DTT RTU (dithiothreitol) pre-weighted, ready-to-dissolve DTT powder for 1M solution P 10 x 154 mg 55 REAGENTS FOR PCR dntp Mix contains 10 mm of each dntp dntp Kit contains separate 100 mm solutions of each dntp 2x PCR Master Mix (ready-to-use 2x concentrated PCR master mix) μl μl x 250 μl x 1000 μl reac. in 50 μl 2x PCR Master Mix Plus ( high specificity ready-to-use 2x concentrated PCR master mix) P 100 reac. in 50 μl 2x PCR Master Mix Plus High GC (ready-to-use 2x concentrated PCR master mix for High GC content templates) G 100 reac. in 50 μl StartWarm 2x PCR Master Mix (ready-to-use 2x concentrated PCR mixture for Hot Start PCR technique) reac. in 50 μl Full pucscreen PCR Master Mix (ready-to-use 2x concentrated mixture for PCR puc clones screening) reac. in 20 μl Full T7Screen PCR Master Mix reac. in 20 μl ((ready-to-use 2x concentrated mixture for PCR pet clones screening) Real Time 2x PCR Master Mix SYBR A (ready-o-use 2x concentrated RealTime-PCR with SYBRGreen) A 100 reac. in 50 μl Real Time 2x PCR Master Mix SYBR B (ready-o-use 2x concentrated RealTime-PCR with SYBRGreen) B 100 reac. in 50 μl Real Time 2x PCR Master Mix SYBR C (ready-o-use 2x concentrated RealTime-PCR with SYBRGreen) C 100 reac. in 50 μl Real Time 2x PCR Master Mix Probe (ready-o-use 2x concentrated RealTime-PCR for fluorescent probes) P 200 reac. in 25 μl Real Time 2x PCR Master Mix EvaGreen (ready-to-use 2x concentrated RealTime-PCR mixture for HRM analysis) G 100 reac. in 50 μl Real Time 2x HS-PCR Master Mix SYBR A Real Time 2x HS-PCR Master Mix SYBR B Real Time 2x HS-PCR Master Mix SYBR C Real Time 2x HS-PCR Master Mix Probe (ready-to-use 2x concentrated RealTime-PCR mixture for Hot Start PCR form fluorescent probes) Real Time 2x HS-PCR Master Mix EvaGreen (ready-to-use 2x concentrated RealTime-PCR mixture for HRM analysis) A 100 reac. in 50 μl B 100 reac. in 50 μl C 100 reac. in 50 μl P 200 reac. in 25 μl G 100 reac. in 50 μl /5

4 LIST REAGENTS FOR REVERSE TRANSCRIPTION TranScriba Kit (First cdna strand synthesis kit with MuMLV Reverse Transcriptase) reactions reactions 210 PRODUCTS FOR GEL ELECTROPHORESIS DNA Marker Ladder loads 42 DNA Marker 2 Low Range FAST loads 42 DNA Marker puc MspI puc19 plasmid DNA digested with MspI restrictase loads DNA Marker puc Mix DNA size standard containig10 blunt-ended fragments ranging from 19 to 1118 bp loads 47 DNA Marker Dra Mix DNA size standard containig17 blunt-ended fragments ranging from 142 to 3794 base pairs loads DNA Marker puc Mix DNA size standard containig12 blunt-ended fragments ranging from 11 to 1444 bp loads 47 DNA Marker puc Mix DNA size standard containig10 blunt-ended fragments ranging from 63 to 2364 bp loads 47 DNA Marker Lambda Hind III bacteriophage Lambda DNA digested with Hind III restrictase loads 18 DNA Marker Lambda BstE II bacteriophage Lambda DNA digested with BstE II restrictase loads 18 DNA Marker Lambda Ava II bacteriophage Lambda DNA digested with Ava II restrictase loads 18 DNA Marker Lambda Pst I bacteriophage Lambda DNA digested with Pst I restrictase loads 18 REAGENT SETS FOR TRANSFORMATION E.coli Transformer Kit for competent cells preparation and transformation of Escherichia coli x 40 transform. 63 Pichia Transformer Kit for competent cells preparation and transformation of Pichia pastoris x 20 transform. 5 Saccharomyces Transformer Kit for competent cells and transformation of Saccharomyces cerevisiae x 20 transform. 5 MEDIA & MEDIA INGREDIENCE LB gr upon gr request LB-Agar (LA) gr upon gr request Yeast Extract gr upon gr request gr Tryptone gr upon gr request gr Peptone gr upon gr request gr Agar gr upon gr request gr 4/5

5 LIST ANTIBIOTICS Ampicillin Sodium g g g 48 Carbenicillin Disodium g g g g 363 Cefotaxim Sodium -1 1 g g g g 1301 Chloramphenicol g g g 47 Doxycyclin Hyclate g g g g 236 G-418 Disulphate g g g g 1443 Hygromycin B g g 1596 Kanamycin Monosulfate g g g g 232 5/5