MOLECULAR SOLUTIONS FOR BIOTECHNOLOGY

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Easter Wilcox
5 years ago
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1 MOLECULAR SOLUTIONS FOR BIOTECHNOLOGY

OUR PCR TECHNOLOGY OUR CAPABILITIES Over 20 years of molecular biology research has given Bioline scientists a deep understanding of the profound influence that buffer chemistry has on DNA polymerase

2 OUR PCR TECHNOLOGY OUR CAPABILITIES Over 20 years of molecular biology research has given Bioline scientists a deep understanding of the profound influence that buffer chemistry has on DNA polymerase performance and reaction kinetics. Through the use of optimal formulations of salts, enhancers and stabilizers, in conjunction with exacting enzyme purification, ultra-high purity nucleotides and strict quality standards, we have developed a robust and powerful amplification platform. Our market-leading SensiFAST and MyTaq systems deliver unparalleled sensitivity, reaction speed and flexibility, and consistently outperform competing products in a wide range of comparison assays. With the highest quality as standard, Bioline reagents enable and accelerate molecular biology in diagnostic, agricultural, biotechnology and pharmaceutical companies around the world. Real-Time PCR Real-Time PCR and RT-PCR mixes Genotyping mix for allelic discrimination High-resolution melt (HRM) mix End-Point PCR Standalone enzymes and mixes Antibody and chemically-mediated hot-start polymerases Long PCR and high-fidelity DNA polymerases Nucleotide Chemistry Superior quality dntps >99% pure as determined by HPLC Validated for the most demanding applications OUR COMMITMENT TO QUALITY From early stage development through manufacturing and delivery, our processes are tightly controlled to ensure consistent manufacture and performance. Consistent with the Bioline commitment to excellence, our manufacturing sites are ISO 9001:2008 certified and are currently operating at the levels required to meet ISO certification, giving you confidence in Bioline as a qualified supplier.

OUR CORE PRODUCTS SensiFAST for Real-Time PCR MyTaq DNA Polymerase for End-Point PCR Singleplex vs.

for high-throughput SNP and multiplex assays One-step kits for first-strand cdna from picograms of total RNA and Real-Time PCR in a single tube, ideal for viral screening

Suitable for a wide range of applications including genotyping, viral load quantification, pathogen detection, oncology, single cell analysis, copy-number variation, quality

Probe Hi-ROX (black) is more sensitive than Supplier Q (red) Industry-leading proprietary buffer formulations for maximum PCR flexibility Designed for fast, GC-rich and multiplex

and stabilizers Available in standard or custom mixes, with or without red gel-loading dye www.bioline.

3 OUR CORE PRODUCTS SensiFAST for Real-Time PCR MyTaq DNA Polymerase for End-Point PCR Singleplex vs. multiplex Amplification of GC rich DNA The fastest commercially available Real-Time PCR mixes, unmatched for sensitivity and enabling detection of low copy pathogens Optimized for high-throughput SNP and multiplex assays One-step kits for first-strand cdna from picograms of total RNA and Real-Time PCR in a single tube, ideal for viral screening Lyophilization flexibility for your kit development requirements Compatible with any pre-designed or custom primer/probe technology Validated with all major Real-Time instruments Suitable for a wide range of applications including genotyping, viral load quantification, pathogen detection, oncology, single cell analysis, copy-number variation, quality control and assay validation Also available in custom formulations Multiplexed qpcr assays with the same high performance as singleplex assays Fast cycling conditions SensiFAST Probe Hi-ROX (black) is more sensitive than Supplier Q (red) Industry-leading proprietary buffer formulations for maximum PCR flexibility Designed for fast, GC-rich and multiplex PCR, ideal for high-throughput Available in antibody-mediated hot-start formulation for increased reaction specificity Reaction buffers contain ultra-high purity dntps, enhancers and stabilizers Available in standard or custom mixes, with or without red gel-loading dye MyTaq Supplier F Supplier N M M Supplier R Supplier T Supplier P M M The robust formulation of MyTaq powers the most demanding assays. Multiplex PCR M Consistently higher yields and sensitivity allow for more uniform multiplexed PCR

4 Ultra-High Purity Nucleotides BULK PACK SIZES FOR HIGH-THROUGHPUT Extended shelf-life of 24 months Supplied in lithium salts for increased resistance to repeated freeze-thaws and microbial contamination Lyophilization flexibility for your kit development requirements RELATED KITS SensiFAST cdna Synthesis Kit A rapid and sensitive method for first-strand cdna synthesis for use in Real-Time PCR studies Unique reverse transcriptase and TransAmp buffer generate consistent first-strand cdna Broad dynamic range, for high- and low-copy targets Real-Time PCR analysis from as little as 1pg of total RNA Unbiased 5 to 3 RNA sequence representation Available in standard or custom formulations and mixes Ultra-pure >99% triphosphate confirmed by HPLC Free from DNase, RNase, Nickase and PCR inhibitors Available in sets, mixes and custom configurations RNA and DNA Extraction Controls Designed to validate the extraction step of diagnostic Real-Time PCR assays. Control is spiked-in prior to extraction, confirming successful extraction Monitoring and validation of DNA or RNA extraction protocols for Real-Time PCR assays Highly specific, minimal interference with sample detection Eliminating false negatives Ideal for blood, urine and sputum samples PRODUCT NAME CAT NO. PACK SIZE NO. OF UNITS/REACTIONS DNA POLYMERASES - FOR REGULAR APPLICATIONS MyTaq DNA Polymerase CSA x 10ml 50,000 Units MyTaq Mix, 2x CSA x 50ml 2000 Reactions MyTaq Red DNA Polymerase CSA x 10ml 50,000 Units MyTaq Red Mix, 2x CSA x 50ml 2000 Reactions DNA POLYMERASES - FOR HOT-START APPLICATIONS MyTaq HS DNA Polymerase CSA x 10ml 50,000 Units MyTaq HS Mix, 2x CSA x 50ml 2000 Reactions IMMOLASE DNA Polymerase CSA x 10ml 50,000 Units cdna SYNTHESIS FOR REAL-TIME PCR SensiFAST cdna Synthesis CSA x 10ml 10,000 Reactions Tetro RT Enzyme CSA x 1.25ml 250,000 Units NUCLEOTIDES 100mM dntp Set CSA x 50ml n/a 100mM dntp Mix CSA x 50ml n/a REAL-TIME PCR DNA KITS SensiFAST Probe No-ROX CSA x 100ml 10,000 Reactions SensiFAST Probe Lo-ROX CSA x 100ml 10,000 Reactions SensiFAST Probe Hi-ROX CSA x 100ml 10,000 Reactions SensiFAST SYBR No-ROX CSA x 100ml 10,000 Reactions SensiFAST SYBR Lo-ROX CSA x 100ml 10,000 Reactions SensiFAST SYBR Hi-ROX CSA x 100ml 10,000 Reactions SensiFAST SYBR & Fluorescein CSA x 100ml 10,000 Reactions SensiFAST Genotyping Lo-ROX CSA x 100ml 10,000 Reactions SensiFAST Genotyping Hi-ROX CSA x 100ml 10,000 Reactions REAL-TIME PCR RNA KITS SensiFAST Probe No-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST Probe Lo-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST Probe Hi-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST SYBR No-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST SYBR Lo-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST SYBR Hi-ROX One-Step CSA x 100ml 10,000 Reactions SensiFAST SYBR & Fluorescein One-Step CSA x 100ml 10,000 Reactions

Procomcure Biotech. PCR Reagents

Procomcure Biotech. PCR Reagents Procomcure Biotech PCR Reagents valid for 2018 VitaTaq DNA Polymerase is a standard Taq DNA polymerase suitable for all common PCR applications like colonypcr, cloning applications, high-throughput PCR