RNA from Any Sample Tel: +49 (0)

Transcription

1 RNA from Any Sample

2 RNA Isolation Overview Zymo Research offers an assortment of products that allow for the simple, rapid, and efficient isolation of total RNA from a variety of biological sources including fresh, frozen, or paraffin-embedded tissues, cultured cells, buccal cells, whole blood, plasma, serum, urine, yeast, or RNA viruses. All of our RNA isolation kits feature Zymo-Spin Column technology, which yields highly concentrated RNA perfect for applications such as microarrays, denaturing-gel electrophoresis, Northern blotting, RT-PCR, and all sensitive downstream applications. Each kit has been optimized for a particular application with specialized, nuclease-free components to ensure: 1) Maximum levels of membrane solubilization and cellular disruption, 2) Total inhibition of nuclease activity, 3) Complete deproteinization of the sample, 4) Efficient isolation and concentration of the RNA, 5) Stabilization and safe storage of the RNA. Samples in TRIzol, TRI Reagent, etc. without phase separation in 7 min. Direct-zol RNA Miniprep Plus Kit Cells Quick-RNA Miniprep Kit Biological Fluids & Tissues Quick-RNA Miniprep Plus Kit Quick-RNA Whole Blood Kit Fixed Tissues Quick-RNA FFPE Kit Microbial Plant Culture Environmental (Fecal, soil, water filtrate and other) Quick-RNA Plant Miniprep Kit Quick-RNA Fungal/ Bacterial Kits Quick-RNA Fecal/ Soil Microbe Kit For RNA isolation in microbiomic studies, see our ZymoBIOMICS product line. Learn more at

3 TRIzol IN RNA OUT Highlights Direct-zol RNA Kits Quick purification of high-quality (DNA-free) total RNA directly from samples stored in TRIzol, TRI Reagent and all other acid-guanidinium-phenol based reagents. Eliminates phase separation and precipitation procedures. Efficient, broad range purification of small and large RNAs from cells, tissues, biological liquids, in vitro transcripts, etc. Ideal for viral inactivation/sample storage. Innovation. Pure & Simple. From TRIzol to RNA in only 7 minutes! Sample in TRIzol Bind RNA directly Eluted RNA Add binding agent to a spin-column ready to use Efficient Small RNA Recovery mirna-seq ncounter 2837 overlapped mirna: r 2 = overlapped mirna: r 2 = [nt] High-quality RNA 4000 mirvana (log 10 ) mirvana (log 10 ) Direct-zol (log 10 ) Direct-zol (log 10 ) (RIN) The data show RNA purified from TRIzol samples using the Direct-zol RNA Miniprep compared to an unbiased method (mirvana, Ambion). Micro-RNA analysis was performed using mirna-seq (MiSeq, Illumina) and a direct hybridization assay (ncounter, Nanostring). High RNA integrity number (RIN > 9; Bioanalyzer (Aligent Technologies Inc.)) indicates high-quality RNA was purified from human epithelial cells using the Direct-zol RNA Kit. R2060, R2061* 50 preps. Direct-zol RNA Microprep Kit R2062, R2063* 200 preps. Accommodates any Sample in TRIzol, TRI Reagent, etc. Direct-zol RNA Miniprep Kit R2050, R2051* 50 preps. R2052, R2053* 200 preps. R2070T 10 preps. Direct-zol RNA Miniprep Plus Kit R2070, R2071* 50 preps. R2072, R2073* 200 preps. Learn more and view additional Direct-zol Kit formats at R2054, R2055* 2 x 96 preps. Direct-zol 96 RNA Kit R2056, R2057* 4 x 96 preps. TRIzol and TRI Reagent are registered trademarks of Molecular Research Center, Inc. U.S. Patent No. 9,051,563 B2 and other pending patents. Direct-zol is a trademark of Zymo Research Corp. *Supplied with TRI Reagent. Magbead formats also available.

4 Quick-RNA Miniprep Plus Kit Highlights High-quality total RNA (including small/micro RNAs) from all tissues, cells, whole blood, and biological fluids. Worry-free sample storage at ambient temperatures with provided DNA/ RNA Shield. DNA-free RNA is ready for use in any downstream application. No organic denaturants! Versatility Ultra-pure, DNA-free RNA Zymo Research Quick-RNA Miniprep Plus Kit Kit 1 Kit 2 Kit 3 M Liver Lung Spleen Brain Muscle Heart ü ü ü ü ü ü û û û ü û ü Liver High-quality total RNA is isolated from various tissue types using the Quick-RNA Miniprep Plus Kit. In comparison, RNA isolated from the same tissue types using three kits recommended by (Agilent 2200 TapeStation ; Red = low quality). Lung Spleen Brain Muscle Heart RNA isolated with the Quick-RNA Miniprep Plus kit is DNA-free. Samples isolated with s kit are provided for comparison. Total RNA was isolated from 10 6 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments. Quality Value DNA Free! Quick-RNA Genomic DNA Quick-RNA Small RNA ( 17 nt) recovery Yes No 28S 18S The Quick-RNA kits yield high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q but not with the Quick-RNA kits. Total RNA was isolated from human epithelial cells (sans DNase treatment). üû DNase I included Yes No gdna removal column included Yes No Proteinase K Yes* No DNA/RNA Shield (for sample storage) *Quick-RNA Miniprep Plus Kit Yes* No Quick-RNA Miniprep Plus Kit Quick-RNA Miniprep Kit (no Proteinase K) R1057 R1058 R1054 R preps. 200 preps. 50 preps. 200 preps. Learn more and view additional Quick-RNA formats at Tel: (949) (0)

5 Quick-RNA Whole Blood Kit Highlights Purify high-quality total RNA (including small/micro RNAs) from whole and partitioned blood samples. Compatible with commonly used anticoagulants (i.e., EDTA, citrate, heparin). DNA-free RNA is ready for use in any downstream application. DNase I included. Worry-free sample storage at ambient temperatures with provided DNA/ RNA Shield. Innovative, Streamlined Workflow Nucleic Acid Stabilization at Ambient Temperature for 30 Days Prior to Purification Whole Blood Plasma Serum PBMCs, Buffy Coat, etc. DNA/RNA Shield (optional storage/transport at ambient temp.) 15 Blood 10 18s rrna C-FOS b-actin IL1-β Zymo-Spin IIICG Column Isolate: Spin Elute 20 C t c-fos 18s rrna IL1-B Add Ethanol Days RNA in blood is effectively stabilized in DNA/RNA Shield at ambient temperature. Zymo-Spin IC Column Purify and Concentrate (optional DNase I treatment) Spin Wash x2 Elute Concentrated Total RNA (including small & micro RNAs) Quick-RNA Whole Blood Kit R preps. Learn more and view additional Quick-RNA formats at Tel: Tel: +49 (949) (0)

6 Quick-RNA FFPE Kit Highlights High performance sample prep technology for high-quality total RNA (including small/micro RNAs) from FFPE tissue samples and sections. DNA-free RNA is ready for use in any downstream application. DNase I included. Streamlined, Innovative Workflow Improved RNA Recovery from FFPE Samples FFPE Tissue 5000 RNA Zymo $ Deparaffinization $ Proteinase K Digestion $ RFU cycles RNA isolated using the Quick-RNA FFPE Kit is high quality and consistently outperforms RNA isolated using procedures (Avg ΔCt = 3.6) as depicted by the RT-PCR amplification curves (n=4). High-quality RNA Zymo Spin Wash Elute $ Ultra-pure Total RNA Zymo-Spin Column RNA isolated with the Quick-RNA FFPE Kit is higher quality compared to procedures. Quality assessed by Agilent 2200 TapeStation. Quick-RNA FFPE Kit R preps. Quick-DNA/RNA FFPE Kit R preps. Learn more and view additional Quick-RNA formats at

7 Quick-RNA Environmental Kits Are you isolating RNA from tough-to-lyse and environmental samples? We offer a variety of kits which feature our superior mechanical lysis, BashingBead, technology. With these kits, RNA can be isolated from samples otherwise resistant to conventional lysis procedures, including solid tissues, plants, seeds, food, arthropods, Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, unicellular or filamentous algae, and protozoa. The result is high-yield, high-quality RNA that is suitable for downstream applications such as RT-PCR and more. Innovation. Pure & Simple. Superior Recovery of RNA from Environmental Samples Pulverize Sample Using ZR BashingBead Lysis Tube Sample 1 Sample 2 Eliminate DNA with Zymo-Spin IIIC Bind, Wash, Elute RNA with Zymo-Spin IIIC Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the Quick-RNA Plant Miniprep Kit. Leaves were minced, then processed using a FastPrep -24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium Markers (Ambion) were used as size standards. Polyphenolic Removal with Zymo-Spin IV-HRC RNA Ladder Zymo Research Supplier A DNA Ladder Clean & Concentrate RNA with Zymo-Spin IC or IIC kb kb DNase I * DNase I Inhibitor-free RNA Total RNA was isolated from equal amounts of E.coli cells containing plasmid DNA (pgem ) using the Quick-RNA Fungal/ Bacterial Microprep Kit or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used. * = genomic (> 10 kb) and plasmid (> 3 kb) DNA contamination DNase I = samples treated with DNase I. Quick-RNA Fecal/Soil Microbe Microprep Kit R preps. Quick-RNA Fungal/Bacterial Microprep Kit R preps. Quick-RNA Fungal/Bacterial Miniprep Kit R preps. Quick-RNA Tissue & Insect Microprep Kit R preps. Quick-RNA Plant Miniprep Kit R preps. Learn more and view additional Quick-RNA formats at

8 Innovation. Pure & Simple. Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision... The Beauty of Science is to Make Things Simple is now truer than ever. Whether it s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance. Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs. Innovation Zymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple and RNA Purification Made Simple, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for highquality plasmid DNA purification, and patented the first RNA purification directly from Trizol without phase separation among many other leading technologies in the industry. Quality We are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality. Customer Service We strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future. Cat. No. B1003 Version 01