TANBead Reagent Kits. Total Solution Provider for Nucleic Acid Extraction FACILITATING YOUR RESEARCH WITH MAGNETIC BEADS

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Jasper Black
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1 TANBead Reagent Kits Total Solution Provider for Nucleic Acid Extraction FACILITATING YOUR RESEARCH WITH MAGNETIC BEADS

DNA Step 6 Release beads Sample Bead DNA Whirl Stirring: A Better Mixing Approach

2 Nucleic Acid Extraction Step 1 Activate beads Step 2 Mix sample with lysis buffer Step 3 Mix sample with beads Step 4 Wash bead-dna in wells 2 through 5 Step 5 Elute DNA Step 6 Release beads Sample Bead DNA Whirl Stirring: A Better Mixing Approach Efficient mixing Larger processing volume Prevention of aerosol cross-contamination Spin Tips

3 ① Assemble auto tubes Auto Tube Reagent Kit Patented Auto Tubes: Flexible sample number ② Add sample Auto Plate Reagent Kit Add sample

4 TANBead Reagent Kits for Nucleic Acid Extraction Type of sample REF Sample Description 611A μl blood or buffy coat Genomic DNA from blood samples Blood 61EA μl blood, frozen blood or buffy coat Frozen blood, buffy coat 612A mg tissue or 2 x 10 6 cells Cultured cells or animal tissues Animal tissue 6T2A mg tissue Enhanced lysis of animal tissues 6K2A mg animal tissue or 2 x 10 5 cells Cultured cells and animal tissues 613A mg plant and fungi tissue Various plant tissues Plant tissue 619A56 Rice Grains of rice can be processed 6K3A mg plant tissues RNA from plant tissues Bacteria 61GA46 Bacterial culture Bacterial DNA and RNA 615A μl serum Designed for HBV Virus 635A μl serum RNA viruses 665A μl serum HCV and other RNA viruses cfdna 61CA μl plasma Designed for cfdna extraction FFPE 61PA μl thick FFPE samples DNA extraction from FFPE samples

5 Animal tissue REF#612A46 provides a simple extraction method to isolate genomic DNA from cells or tissues. Samples need to be homogenized with lysis buffer and then incubated at room temperature for 10 minutes prior to automated nucleic acid extraction by TANBead s automated magnetic bead handling systems. The workflow saves experimental time and enhances consistency and reproductivity of DNA isolation. It is suitable for laboratories with high-throughput requirements. REF#6T2A46 is dedicated to isolating DNA from tissues that are difficult to be lysed. Samples are pre-treated with proteinase K before automated nucleic acid extraction by TANBead s automated magnetic bead handling systems. The workflow saves experimental time and enhances consistency and reproductivity of DNA isolation. The isolated nucleic acid samples can be used in subsequent applications, such as the polymerase chain reaction (PCR), real-time PCR, and other clinical tests. It is suitable for laboratories with high-throughput requirements. REF#6K2A46 is employed in RNA isolation from a variety of animal cells or tissues. Automated nucleic acid extraction by TANBead s automated magnetic bead handling systems saves experimental time and enhances consistency and reproductivity of RNA isolation. The isolated nucleic acid samples can be used in subsequent applications, such as real-time PCR, and other clinical tests. It is suitable for laboratories with high-throughput requirements.

Plant tissue REF#619A56 with TANBead Nucleic Acid Extractor simplifies the process of isolating rice DNA. The process of grinding grain and cell lysis are completely automated.

REF#613A46 is suitable for nucleic acid extraction from various plant tissues, including plant leaves, mushrooms, maize, wheat, and soybeans.

6 Plant tissue REF#619A56 with TANBead Nucleic Acid Extractor simplifies the process of isolating rice DNA. The process of grinding grain and cell lysis are completely automated. The workflow saves experimental time and enhances consistency and reproductivity of DNA isolation. It is ideal for applications such as germplasm identification. REF#613A46 is suitable for nucleic acid extraction from various plant tissues, including plant leaves, mushrooms, maize, wheat, and soybeans. REF#6K3A46 is suitable for RNA isolation from plant tissues such as frozen and fresh stems, leaves, flowers, and seeds. It can be employed to extract viral RNA. Automated nucleic acid extraction by TANBead s automated magnetic bead handling systems saves experimental time and enhances consistency and reproductivity of RNA isolation. Nucleic acid extracted from various plant tissues are analyzed by agarose gel electrophoresis.

Bacteria TANBead Nucleic Acid Extraction Kit REF#61GA46 provides a simple and convenient method for DNA isolation from Gram-positive and Gram-negative bacteria.

DNA extraction is automated by TANBead Nucleic Acid Extractor. The workﬂow saves experimental time and enhances consistency and reproductivity of DNA isolation.

M 1 2 3 4 5 6 7 8 Nucleic acid extracted from lactobacillus bacteria are analyzed by agarose gel electrophoresis.

7 Bacteria TANBead Nucleic Acid Extraction Kit REF#61GA46 provides a simple and convenient method for DNA isolation from Gram-positive and Gram-negative bacteria. The isolated nucleic acid can be analyzed directly by PCR, restriction digestion, and PFGE (pulsed-ﬁeld gel electrophoresis). DNA extraction is automated by TANBead Nucleic Acid Extractor. The workﬂow saves experimental time and enhances consistency and reproductivity of DNA isolation. It is suitable for laboratories with high-throughput requirements. M Nucleic acid extracted from lactobacillus bacteria are analyzed by agarose gel electrophoresis. M Nucleic acid from various bacteria (Bacillus, Microbacterium, Massilia, Paenibacillus, Corynebacterium, Escherichia, Novosphingomonas, Cupriavidus, Duganella, Flavobacterium, Lactobacillus, Weissella, Leuconostoc, Burkholderia) are extracted. 16S ribosomal RNA genes are ampliﬁed by PCR and analyzed in agarose gel electrophoresis.

8 Blood REF#611A46 is a blood DNA extraction kit that simplifies pretreatment steps. This kit is mainly suitable for extracting DNA samples from fresh whole blood, buffy coat, or blood mixed with EDTA or citrate. The isolated nucleic acid samples can be subjected to subsequent application directly, such as the polymerase chain reaction (PCR), enzyme reactions, HLA-typing, and other clinical tests. TANBead Blood DNA Extraction Kit REF#61EA46 is a blood DNA extraction kit. It can be applied to process diverse blood samples, including frozen blood and buffy coats. Samples are subject to pre-treatment with proteinase K before automated nucleic acid extraction by TANBead s automated magnetic bead handling systems. The isolated nucleic acid samples can be subjected to subsequent application directly, such as the polymerase chain reaction (PCR), enzymatic reactions, HLA-typing, and other clinical tests.

Blood Blood samples TANBead other NA extraction kits The nucleic acids isolated by

M 1 2 3 4 5 6 M The nucleic acids isolated by TANBead blood extraction kits are of high

method. Lane Description A280/A260 ratio DNA yield (μg) 1 TANBead test 1 1.80 2.

9 Blood Blood samples TANBead other NA extraction kits The nucleic acids isolated by TANBead Blood DNA extraction kits are less contaminated with RNA. M M The nucleic acids isolated by TANBead blood extraction kits are of high purity and less fragmented in comparison to the nucleic acid isolated by the spin-column method. Lane Description A280/A260 ratio DNA yield (μg) 1 TANBead test TANBead test TANBead test Q brand test Q brand test Q brand test

10 Virus REF#615A46 provides an effective way of extracting viral DNA from serum. In addition to extract DNA samples from general common viruses, the kits are effective for the extraction of hepatitis B virus DNA. Samples are treated with proteinase K prior to automated nucleic acid extraction process by TANBead Nucleic Acid Extractor. The extracted DNA are of high purity and can be applied directly in subsequent qualitative and quantitative assays. HBV detection by Real-Time PCR: Linear performance across a wide concentration range (102 IU/ml ~ 108 IU/ml). Nucleic acid extracted by 10 different batches of 615A46 are analyzed by RT-PCR. The data present the stability of product quality.

11 Virus TANBead Viral Nucleic Acid Extraction Kit REF#635A46 is suitable for isolating a variety of viral nucleic acids, including the Japanese encephalitis, dengue, avian influenza, and Epstein-Barr viruses. Compared to conventional methods, these kits have a simplified procedure. 200 μl of serum are pre-treated with proteinase K before automated nucleic acid extraction by TANBead s automated magnetic bead handling systems. The isolated nucleic acids are of excellent quality with no contamination of proteins, nucleases, or inhibitors and can be directly applied in subsequent experiments, such as polymerase chain reactions (PCR), real-time polymerase chain reactions (Real-Time PCR), and reverse transcription polymerase chain reactions (RT-PCR). This kit is suitable for clinical research and inspection units. REF#665A46 is designed for the extraction of a variety of viral nucleic acids, including the hepatitis C, hepatitis B, and influenza viruses. Samples are treated with proteinase K prior to the automated nucleic acid extraction process by TANBead Nucleic Acid Extractor. The isolated nucleic acids are of excellent quality with no contamination of proteins, nucleases, or inhibitors. The isolated nucleic acids can be directly applied in subsequent experiments, such as polymerase chain reactions (PCR), real-time polymerase chain reactions (Real-Time PCR), and reverse transcription polymerase chain reactions (RT-PCR). This kit is suitable for clinical research and inspection units.

12 cfdna REF#61CA46 is designed for isolating cell free DNA (cfdna). Samples can be treated with proteinase K prior to the automated nucleic acid extraction process by TANBead s automated magnetic bead handling systems. Nucleic acids extracted can be analyzed by downstream applications, such as real-time PCR. With high sensitivity, this reagent kit can be applied for clinical diagnostics and research. Analysis of cfdna isolated from 600 ul plasma by TANBead cfdna kit.

After pre-treatment of proteinase K and reverse formaldehyde modification of nucleic acids in incubation buffer, nucleic acid can be extracted by thetanbead Nucleic Acid Extractor as a fully

13 FFPE REF#61PA46 is dedicated to the isolation of DNA from formalin-fixed paraffin-embedded (FFPE) tissue sections. Instead of using hazardous organic solvents such as xylene, mineral oil is used for deparaffinization of FFPE. After pre-treatment of proteinase K and reverse formaldehyde modification of nucleic acids in incubation buffer, nucleic acid can be extracted by thetanbead Nucleic Acid Extractor as a fully automated process. The extracted nucleic acids are ready for downstream applications. The simple and automated extraction process is suitable for laboratories with high-throughput requirements. Pig liver standard FFPE sample (pig liver) A B C D E Nucleic acid extracted (set C) from FFPE sample is as intact as those extracted from fresh pig liver.

14 Sample Total Solution Provider for Nucleic Acid Extraction 10F., No. 95, Xinpu 6th St., Taoyuan Dist., Taoyuan City 330, Taiwan T F E.

ssdna/rna Clean & Concentrator Catalog Nos. D7010 & D7011

ssdna/rna Clean & Concentrator Catalog Nos. D7010 & D7011 INSTRUCTION MANUAL Catalog Nos. D7010 & D7011 Highlights Quick (10 minute) method for separating, cleaning and concentrating single stranded DNA or RNA. Ideal for non-enzymatic elimination of genomic DNA,