Forensic DNA Evidence: Collection, Mixtures, and Degradation
|
|
|
- Alexina Wilkinson
- 5 years ago
- Views:
Transcription
1 Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences Forensic DNA Evidence: Collection, Mixtures, and Degradation Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu Follow this and additional works at: Part of the Biology Commons, Medical Sciences Commons, and the Systems Biology Commons Repository Citation Krane, D. E. (2004). Forensic DNA Evidence: Collection, Mixtures, and Degradation.. This Presentation is brought to you for free and open access by the Biological Sciences at CORE Scholar. It has been accepted for inclusion in Biological Sciences Faculty Publications by an authorized administrator of CORE Scholar. For more information, please contact corescholar@
2 Forensic DNA evidence: collection, mixtures and degradation Dan E. Krane Biological Sciences, Wright State University, Dayton OH Forensic Bioinformatics (
3 Three generations of DNA testing RFLP AUTORAD Allele = BAND DQ-alpha TEST STRIP Allele = BLUE DOT Automated STR ELECTROPHEROGRAM Allele = PEAK
4 DNA content of biological samples: Type of sample Blood stain 1 cm in area stain 1 mm 2 in area Semen Postcoital vaginal swab Hair plucked shed Saliva Urine 2 Amount of DNA 30,000 ng/ml 200 ng 2 ng 250,000 ng/ml 0-3,000 ng ng/hair 1-12 ng/hair 5,000 ng/ml 1-20 ng/ml
5 Basic terminology: Genetics DNA Polymorphism ( many forms ) Regions of DNA which differ from person to person Locus (plural = loci) Site or location on a chromosome Allele Different variants which can exist at a locus DNA Profile The combination of alleles for an individual
6 Basic terminology: Technology Amplification or PCR (Polymerase Chain Reaction) A technique for replicating DNA in the laboratory ( molecular Xeroxing ) Region to be amplified defined by PRIMERS Can be color coded Electrophoresis A technique for separating molecules according to their size
7 Short tandem repeat STR Describes a type of DNA polymorphism in which: a DNA sequence repeats over and over again and has a short (usually 4 base pair) repeat unit A length polymorphism -- alleles differ in their length 3 repeats: AATG AATG AATG 4 repeats: AATG AATG AATG AATG 5 repeats: AATG AATG AATG AATG AATG 6 repeats: AATG AATG AATG AATG AATG AATG
8 Reading an electropherogram Peaks correspond to alleles BLUE D3 vwa FGA Amelogenin XX = female XY = male Red = ROX size standard Electropherogram Amelogenin D8 D21 D18 GREEN D5 D13 D7 YELLOW bps 150 RED 160
9 Automated STR Test
10 Crime Scene Samples & Reference Samples Extract and purify DNA Differential extraction in sex assault cases separates out DNA from sperm cells
11 Extract and Purify DNA Add primers and other reagents
12 PCR Amplification DNA regions flanked by primers are amplified Groups of amplified STR products are labeled with different colored dyes (blue, green, yellow)
13 The ABI 310 Genetic Analyzer: SIZE, COLOR & AMOUNT
14 Amplified STR DNA injected onto column Electric current applied DNA pulled towards the positive electrode ABI 310 Genetic Analyzer: Capillary Electrophoresis DNA separated out by size: Large STRs travel slower Small STRs travel faster Detector Window Color of STR detected and recorded as it passes the detector
15 Profiler Plus: Raw data
16 Statistical estimates: the product rule x x 2 = 0.1
17 Statistical estimates: the product rule x 1 in 10 1 in in 20 = 0.1 x 1 in in 14 1 in 81 x x 1 in 22,200 x x 1 in in 17 1 in 16 1 in 113,400 1 in 79,531,528,960,000,000 1 in 80 quadrillion 1 in 31,552
18 What more is there to say after you have said: The chance of a coincidental match is one in 80 quadrillion?
19 What more is there to say after you have said: The chance of a coincidental match is one in 80 quadrillion? Two samples really do have the same source Samples match coincidentally An error has occurred
20 Sources of ambiguity in DNA testing results Mixtures: deconvolution and relatives Degradation, inhibition Background noise Stutter (n+4) Pull-up Spikes and blobs
21 Opportunities for subjective interpretation?
22 Opportunities for subjective interpretation? D3: 12, 17 vwa: 15, 17 FGA: 22, 26
23 Sources of ambiguity in DNA testing results Mixtures: deconvolution and relatives Degradation, inhibition Background noise Stutter (n+4) Pull-up Spikes and blobs
24 Mixed DNA samples
25 How many contributors to a mixture? if analysts can discard a locus? Maximum # of alleles observed in a 3 person mixture # of occurrences Percent of cases , ,526, ,498, ,078, ,938, ,526, ,702, There are 45,139,896 possible different 3-way mixtures of the 648 individuals in the MN BCI database.
26 Sources of ambiguity in DNA testing results Mixtures: deconvolution and relatives Degradation, inhibition Background noise Stutter (n+4) Pull-up Spikes and blobs
27 Accounting for relatives 20.00% 18.00% 16.00% 14.00% Percent of Total 12.00% 10.00% 8.00% 6.00% Synthetic Cousins Siblings 4.00% 2.00% 0.00% Number of Shared Alleles
28 Likelihood ratios for allele sharing: % 90.00% 80.00% 70.00% Likelihood 60.00% 50.00% 40.00% 30.00% 20.00% 10.00% Synthetic Cousins Siblings 0.00% Number of Shared Alleles
29 Sources of ambiguity in DNA testing results Mixtures: deconvolution and relatives Degradation, inhibition Background noise Stutter (n+4) Pull-up Spikes and blobs
30 Degradation S M A L L L A R G E When biological samples are exposed to adverse environmental conditions, they can become degraded Warm, moist, sunlight, time Degradation breaks the DNA at random Larger amplified regions are affected first Classic ski-slope electropherogram Degradation is unusual.
31 Degradation The Leskie Inquest Undegraded samples can have ski-slopes too. How negative does a slope have to be to an indication of degradation? Experience, training and expertise. Positive controls should not be degraded.
32 Degradation The Leskie Inquest DNA profiles in a rape and a murder investigation match. Everyone agrees that the murder samples are degraded. If the rape sample is degraded, it could have contaminated the murder samples. Is the rape sample degraded?
33 Degradation The Leskie Inquest
34 Sources of ambiguity in DNA testing results Mixtures: deconvolution and relatives Degradation, inhibition Background noise Stutter (n+4) Pull-up Spikes and blobs
35 Background noise
36 Stutter peaks
37 Pull-up (software differences) Advanced Classic
38 Spikes and blobs
39 Spikes and blobs blob Peak Area Peak area spike Peak height Peak Height Blob: Peak Area / Peak Height > 10 + Spike: Peak Area / Peak Height < 4.5 -
40 Resources Books Forensic DNA Typing by John M. Butler (Academic Press) Internet Applied Biosystems Website: (see human identity and forensics) Forensic Bioinformatics Website: STR base: (very useful) Scientists Larry Mueller (UC Irvine) Simon Ford (Lexigen, Inc. San Francisco, CA) William C. Thompson (UC Irvine) William Shields (SUNY, Syracuse, NY) Marc Taylor (Technical Associates, Ventura, CA) Keith Inman (Forensic Analytical, Haywood, CA) Testing laboratories Technical Associates (Ventura, CA) Indiana State Police (Indianapolis, IN) Other resources Forensic Bioinformatics (Dayton, OH)
Evaluating Forensic DNA Evidence
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 6-18-2005 Evaluating Forensic DNA Evidence Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu
Generating Forensic DNA Profiles
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 12-2012 Generating Forensic DNA Profiles Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu
Essential Elements of a Defense-Review of DNA Testing Results
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 10-11-2006 Essential Elements of a Defense-Review of DNA Testing Results Dan E. Krane Wright State University
Essential Elements of a Defense-Review of DNA Testing Results
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 5-23-2007 Essential Elements of a Defense-Review of DNA Testing Results Dan E. Krane Wright State University
Current Issues in Forensic DNA Profiling
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 9-2002 Current Issues in Forensic DNA Profiling Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu
DNA 101. DNA 101:Kate Philpott and Jennifer Friedman
DNA 101 DNA 101:Kate Philpott and Jennifer Friedman DNA found in cells of your body cell nucleus DNA Found in cells once they leave your body too CONSIDER THE SOURCE Blood* Semen* Hair Bone Saliva* Urine*
Empirical Analysis of the STR Profiles Resulting from Conceptual Mixtures
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 2-22-2007 Empirical Analysis of the STR Profiles Resulting from Conceptual Mixtures Dan E. Krane Wright
What is DNA? Deoxyribonucleic Acid The inherited genetic material that makes us what we are
DNA Basic Genetics What is DNA? DNA is Deoxyribonucleic Acid The inherited genetic material that makes us what we are DNA in the Cell Human Genome ~3 billion base pairs of DNA 30,000-35,000 genes Population-each
Evaluating Forensic DNA Evidence - A Day-Long Workshop
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 10-13-2007 Evaluating Forensic DNA Evidence - A Day-Long Workshop Dan E. Krane Wright State University
Genetic Identity. Steve Harris SPASH - Biotechnology
Genetic Identity Steve Harris SPASH - Biotechnology Comparison of Organisms ORGANISM GENES BASE PAIRS Lambda Phage 40 50,000 E.coli 400 5,000,000 Yeast 13,000 15,000,000 Human 20,000 3,000,000,000 (3 billion)
Procedure for Casework DNA Interpretation
Procedure for Casework DNA Interpretation 1.0 Purpose The purpose of this document is to provide guidelines for the interpretation of autosomal DNA results when amplified with Identifiler Plus. 2.0 Scope
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database Margaret Kline Applied Group Biochemical Science Division Chemical Science and Technology Laboratory National Institute
FORENSIC GENETICS. DNA in the cell FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS. Sources of biological evidence
FORENSIC GENETICS FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS Establishing human corpse identity Crime cases matching suspect with evidence Paternity testing, even after
Southern hybridization technique
Southern hybridization technique DNA fingerprint analysis is based on the "Southern" hybridization technique. In this method: DNA fingerprinting, also termed DNA profile analysis is based on the use of
Internal Validation of the Promega PowerPlex Fusion System with the Applied Biosystems 3130xl Genetic Analyzer
Internal Validation of the Promega PowerPlex Fusion System with the Applied Biosystems 3130xl Genetic Analyzer Roy Al Ahmar, B.S. Marshall University Forensic Science Center DNA Laboratory Overview What
Procedure for GeneMapper ID-X for Casework
Procedure for GeneMapper ID-X for Casework 1.0 Purpose-This procedure specifies the steps for performing analysis on DNA samples amplified with AmpFlSTR Identifiler Plus using the GeneMapper ID-X (GMID-X)
Procedure for GeneMapper ID-X for Casework
Procedure for GeneMapper ID-X for Casework 1.0 Purpose-This procedure specifies the steps for performing analysis on DNA samples amplified with AmpFlSTR Identifiler Plus using the GeneMapper ID-X (GMID-X)
Virginia and the New SWGDAM Guidelines
DNA Mixture Interpretation Workshop Brad Jenkins March 16, 2011 Virginia and the New SWGDAM Guidelines Biology Program Manager, Virginia Department of Forensic Science NIJ Disclaimer This project was supported
A Rape on a Minor Victim Under POCSO Act 2012 Investigation Through DNA Analysis Technique A Case Study
A Rape on a Minor Victim Under POCSO Act 2012 Investigation Through DNA Analysis Technique A Case Study Rohidas K. Mundhe, Rajendra S. Mali, Sangeeta V. Ghumatkar and Madhukar K. Malve Abstract DNA profiling
Mutations during meiosis and germ line division lead to genetic variation between individuals
Mutations during meiosis and germ line division lead to genetic variation between individuals Types of mutations: point mutations indels (insertion/deletion) copy number variation structural rearrangements
Unit 4-DNA Analysis Review Guide
Name: KEY Match the term on the right with the definition on the left. Unit 4-DNA Analysis Review Guide 1. A procedure used to determine the order of the base pairs that make up a DNA molecule E 2. These
STR DNA Data Analysis. Genetic Analysis
STR DNA Data Analysis Genetic Analysis Once a DNA profile is generated, how does one interpret the data? DNA data analysis is based on alleles detected DNA data analysis rarely can tell you time of deposit
Developmental Validation of Amplicon Rx, a post-pcr Purification System
Developmental Validation of Amplicon Rx, a post-pcr Purification System Introduction Allelic drop out and allele peaks below analytical threshold are common observation in forensic DNA analysis. These
Overview. Background ~30 min. Lab activity ~50 min. DNA profiling Polymerase Chain Reaction (PCR) Gel Electrophoresis PCR
Overview Day 1: Tuesday Introduction to DNA profiling How do we use DNA to solve crimes? Background Polymerase Chain Reaction (PCR) Gel Electrophoresis Set up PCR Day 2: Wednesday Make and Run Agarose
The ABC s of DNA. Jerome F. Buting BUTING, WILLIAMS & STILLING, S.C. Wisconsin State Public Defender Annual Conference November 12, 2015
The ABC s of DNA Jerome F. Buting BUTING, WILLIAMS & STILLING, S.C. Wisconsin State Public Defender Annual Conference November 12, 2015 Now what? The State has DNA evidence. Discovery Checklist Demand
STR Interpretation Guidelines
Introduction: STR Interpretation Guidelines The interpretation of results in casework is necessarily a matter of professional judgment and expertise. Not every situation can or should be covered by a pre-set
DNA Mixture Interpretation Workshop Dr Chris Maguire. Identification and resolution of DNA mixtures
DNA Mixture Interpretation Workshop Dr Chris Maguire Identification and resolution of DNA mixtures Introduction Mixture analysis models Pros & Cons Recognizing a DNA Mixture Extra peaks Peak imbalance
Chapter 7 DNA Fingerprinting By the end of this chapter you will be able to:
Chapter 7 DNA Fingerprinting By the end of this chapter you will be able to: explain how crime scene evidence is collected and processed to obtain DNA describe how radioactive probes are used in DNA fingerprinting
DNA. Evidence. How is DNA be used to solve crimes?
DNA Evidence How is DNA be used to solve crimes? How is DNA used as evidence? Each person s DNA is different from other people (except identical twins). DNA collected from a crime scene can either link
AmpFlSTR Identifiler PCR Amplification Kit
Product Bulletin Human Identification AmpFlSTR Identifiler PCR Amplification Kit Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube Incorporation of the same proven, reliable
Product Catalog 2012 AmplGen Ltd.
Professional solutions for Genetic Human Identification and Molecular Diagnostics Product Catalog 2012 AmplGen Ltd. Riga, Lativa 2 Catalog AmplGen 2012, ver. #02 Dear colleagues, AmplGen is new European
GlobalFiler TM Extra Cycle Evaluation Report
GlobalFiler TM Extra Cycle Evaluation Report Table of Content Chapter 1 Summary 1. Introduction... 2 2. Systems, Kits and Conditions... 3 3. Studies... 4 Chapter 2 Minimum Threshold and Background Study
Basic Steps of the DNA process
As time pasted technology has improve the methods of analyzing DNA. One of the first methods for the analysis of DNA is known as Restriction Fragment Length Polymorphism (RFLP). This technique analyzed
Centre of Forensic Sciences
Centre of Forensic Sciences Technical Information Sheets DNA Information Introduction DNA is the genetic blueprint of life and is packaged into paired structures known as chromosomes. One from each pair
!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"!"
STR ANALYSIS BY CAPILLARY ELECTROPHORESIS: DEVELOPMENT OF INTERPRETATION GUIDELINES FOR THE PROFILER PLUS AND COFILER SYSTEMS FOR USE IN FORENSIC SCIENCE Deborah Hobson, Jill Smerick, and Jenifer Smith
AmpFlSTR COfiler. PCR Amplification Kit. User Bulletin
AmpFlSTR COfiler PCR Amplification Kit User Bulletin Copyright 2006, Applied Biosystems. Printed in the U.S.A. For Research, Forensic or Paternity Use Only. Not for use in diagnostic procedures. NOTICE
Report of Analyzing Short Tandem Repeats for Parentage Testing
1 Alex Michael Tseng Department of Forensic Medicine, College of Medicine, National Taiwan University Report of Analyzing Short Tandem Repeats for Parentage Testing Introduction In the three billion letter
Further Reading - DNA
Further Reading - DNA DNA BACKGROUND What is DNA? DNA (short for deoxyribonucleic acid ) is a complex molecule found in the cells of all living things. The blueprint for life, DNA contains all the information
The Evolution of Short Tandem Repeat (STR) Multiplex Systems
Ann M. Lins, Cynthia J. Sprecher, Katherine A. Micka, Dawn R. Rabbach, Jennifer A. Taylor, Jeffrey W. Bacher, Laura F. Hennes, Allan Tereba, Trent Gu, Elizabeth A. Amiott, Nadine A. Nassif and James W.
AmpF STR NGM PCR Amplification Kit - Overview
AmpF STR NGM PCR Amplification Kit - Overview The most advanced STR kits optimized for analysis of forensic casework and database samples in Europe Data Quality Worth Sharing! 9/7/2011 Life Technologies
..C C C T C A T T C A T T C A T T C A T T C A..
Polymerase Chain Reaction Lab: a Forensic Application INTRODUCTION PCR (polymerase chain reaction) is a technique that scientists use to amplify particular segments of DNA. This process can produce large
Investigator Quantiplex HYres Kit
These specifications are for the procurement of a validation study at GBI Division of Forensic Sciences facilities of two commercially available kits used in forensic DNA analysis. The two kits to be validated
January 07, (adenine, guanine, cytosine, thymine)
(adenine, guanine, cytosine, thymine) DNA at Work - DNA is used to make proteins - proteins are made by linking amino acids (there are 20 possible amino acids) - sequence of amino acids determines shape/function
Analysis in Forensic Science
Chapter 16 Gene Cloning & DNA Analysis in Forensic Science 1. DNA analysis in identification of crime suspects 2. Studying kinship by DNA profiling 3. Sex identification by DNA analysis Forensic science
TWO YEARS LATER: A REFLECTION ON THE IMPLEMENTATION OF STRMIX IN A HIGH THROUGHPUT DNA LABORATORY
TWO YEARS LATER: A REFLECTION ON THE IMPLEMENTATION OF STRMIX IN A HIGH THROUGHPUT DNA LABORATORY Zane Kerr (corresponding author) New South Wales Forensic & Analytical Science Service, NSW Health Pathology,
STRmix Implementation and Internal Validation-FUSION. Erie County Central Police Services Forensic Laboratory
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 STRmix Implementation and Internal Validation-FUSION Erie County Central Police Services Forensic Laboratory
Data Quality Worth Sharing
Product Bulletin Human Identification AmpFlSTR NGM and NGM SElect PCR Amplification Kits Next generation amplification chemistries including the 5 new loci from the expanded European Standard Set Enhanced
Capillary Electrophoresis in DNA Analysis
Capillary Electrophoresis in DNA Analysis john.butler@nist.gov Troubleshooting the ABI 310 NEAFS Workshop Mystic, CT September 29-30, 2004 Dr. John M. Butler Dr. Bruce R. McCord mccordb@fiu.edu Outline
Forensic Applications of CE and MPS
Forensic Biology Polymorphic Enzyme Single Locus PCR RFLP Massively Parallel Sequencing (MPS) Ion Chef System Ion S5 System Ion Chef System Prepare barcoded DNA libraries with Applied Biosystems Precision
Genetics Lecture 16 Forensics
Genetics Lecture 16 Forensics DNA Forensics Genetics is arguably the most influential science today dramatically affecting technologies in fields as diverse as agriculture, archaeology, medical diagnosis,
ASB Standard 048, First Edition Wildlife Forensic DNA Standard Procedures
ASB Standard 048, First Edition 2018 Wildlife Forensic DNA Standard Procedures Wildlife Forensic DNA Standard Procedures ASB Approved Xxxxx 2018 ANSI Approved Xxxxx 2018 410 North 21st Street Colorado
Training on STR Typing Using Commercial Kits and ABI 310/3100
Training on STR Typing Using Commercial Kits and ABI 310/3100 Margaret C. Kline, Janette W. Redman, John M. Butler National Institute of Standards and Technology October 22-26, 2001 Human Identity Testing
DNA: THE INDISPENSIBLE FORENSIC SCIENCE TOOL
Chapter 9 DNA: THE INDISPENSIBLE TOOL By Richard Saferstein Upper Saddle River, NJ 07458 1 Chapter 9 DNA Fingerprinting By the end of this chapter you will be able to: explain how crime scene evidence
DNA, or Deoxyribonucleic Acid, is the genetic material in our cells. No two people (except identical twins) have the
DNA, or Deoxyribonucleic Acid, is the genetic material in our cells. No two people (except identical twins) have the exact same DNA. DNA patterns from four sets of twins which are identical? DNA fingerprinting
Computational Methods for the Objective Review of Forensic DNA Testing Results
Wright State University CORE Scholar Browse all Theses and Dissertations Theses and Dissertations 2007 Computational Methods for the Objective Review of Forensic DNA Testing Results Jason R. Gilder Wright
CHAPTER 13: DNA TYPING NOW AND BEFORE
CHAPTER 13: DNA TYPING NOW AND BEFORE EVIDENCE Each of us is genetically unique, and there are many cases in which it is convenient to make use of our genetic individuality: for parentage analysis, identification
STRmix Implementation and Internal Validation Erie County Central Police Services Forensic Laboratory
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 STRmix Implementation and Internal Validation-2015 Erie County Central Police Services Forensic Laboratory
Artifacts Identified Post-Developmental Validation: AmpFLSTR Identifiler Plus PCR Amplification Kit
March 29, 2018 TECHNICAL NOTE Artifacts Identified Post-Developmental Validation: AmpFLSTR Identifiler Plus PCR Amplification Kit The purpose of this document is to assist with data interpretation by providing
TWGDAM Validation of AmpF STR PCR Amplification Kits for Forensic DNA Casework
Cydne L. Holt, 1 Ph.D.; Martin Buoncristiani, 2 M.P.H.; Jeanette M. Wallin, 1 M.P.H.; Theresa Nguyen, 1 B.S.; Katherine D. Lazaruk, 1 Ph.D.; and P. S. Walsh, 1 M.P.H. TWGDAM Validation of AmpF STR PCR
GeneMarker HID Software Review
GeneMarker HID Software Review Grayson D. Amick Richland County Sheriff s Department, Columbia, South Carolina Publication Date: 2015 Introduction The use of a new software tool for the analysis of short
DNA typing of human cell lines: historical perspective Yvonne Reid, PhD Collection/Research Scientist ATCC Cell Biology
DNA typing of human cell lines: historical perspective Yvonne Reid, PhD Collection/Research Scientist ATCC Cell Biology Outline Molecular techniques for the authentication of human cell lines Mechanism
An Introduction to Forensic DNA Analysis
2/7/2013 HC70A: Genetic Engineering in Medicine, Agriculture & Law 1 UCLA Dr. Bob Goldberg HC70A: Genetic Engineering in Medicine, Agriculture & Law An Introduction to Forensic DNA Analysis Presented by
CSI TEST. Ref. PCR detectives (4 practices) 1. EXPERIMENT OBJETIVE 2. BACKGROUND INFORMATION
CSI TEST Ref. PCR detectives (4 practices) 1. EXPERIMENT OBJETIVE This practice introduces students to using DNA and PCR to simulate how DNA obtained from a hair or saliva sample from a crime scene can
Forensic DNA Testing. Forensic DNA Testing. Maj Gen (R) Suhaib Ahmed, HI (M)
Maj Gen (R) Suhaib Ahmed, HI (M) DNA from no two individuals except identical twins is alike. The person to person differences in DNA can be discovered by PCR amplification and genomic sequencing. The
4.1. Genetics as a Tool in Anthropology
4.1. Genetics as a Tool in Anthropology Each biological system and every human being is defined by its genetic material. The genetic material is stored in the cells of the body, mainly in the nucleus of
Laboratory Exercise 4. Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis.
Laboratory Exercise 4 4 Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis B A C K G R O U N D The human genome contains over 3000 million base pairs, which are distributed
DNA Analysis Students will learn:
DNA Analysis Students will learn: That DNA is a long-chain polymer found in nucleated cells, which contain genetic information. That DNA can be used to identify or clear potential suspects in crimes. How
Guidelines and Considerations for Evaluating or Validating Extraction Chemistry
Guidelines and Considerations for Evaluating or Validating Extraction Chemistry Introduction In order to evaluate a DNA extraction method effectively, it is imperative to consider all aspects of chemistry
RESULTS FROM THE 1999 NIST MIXED-STAIN STUDY #2: DNA QUANTITATION, DIFFERENTIAL EXTRACTION, AND IDENTIFICATION OF THE UNKNOWN CONTRIBUTORS
RESULTS FROM THE 1999 NIST MIXED-STAIN STUDY #2: DNA QUANTITATION, DIFFERENTIAL EXTRACTION, AND IDENTIFICATION OF THE UNKNOWN CONTRIBUTORS Margaret C. Kline, Janette W. Redman, David L. Duewer, and Dennis
DNA Profiling with PCR
Name: DNA Profiling with PCR OBJECTIVES To review the structure and function of DNA. Understand and perform the polymerase chain reaction (PCR) To gain experience using the micropipettes, thermocycler,
Laboratory Validation. Chapter 16
Laboratory Validation Chapter 16 Importance The DNA profile is used to convict or free a suspect It must be perfect! No mistakes like we have in regular laboratories all the time Also DNA evidence must
The state of Illinois has a grand tradition of corrupt politicians (Re: Former governor Rod Blagojevich.)
Biology 321 May 20, 2011 DNA fingerprinting The state of Illinois has a grand tradition of corrupt politicians (Re: Former governor Rod Blagojevich.) 1 2 Innocence Project http://www.innocenceproject.org/know/
General DNA Information
1 Use of this PPT This PPT has lots of information. I may or may not discuss each slide. Use the information to answer questions in your Question Packets. The packets are the study guide for the tests
Upgrading the Workflow: Enhancing Information Recovery from Forensic Evidence. The world leader in serving science
Upgrading the Workflow: Enhancing Information Recovery from Forensic Evidence The world leader in serving science The GlobalFiler Kit: Going Further for Casework Powered by 6-Dye Chemistry 2 What is the
Understanding PCR. Total allelic product Peak height Back stutter Forward stutter Variability in peak height
Understanding PCR Total allelic product Peak height Back stutter Forward stutter Variability in peak height 1 Total allelic product (TAP) Total allelic product, T a = height of allele plus height of stutter
CONCEPT TRAINING DOCUMENT
CONCEPT TRAINING DOCUMENT DOCUMENT TYPE : REF. CODE: ISSUE NO: ISSUE DATE: POLICY ENFSI DNA 001 November 2010 WORKING GROUP 1. INTRODUCTION One of the goals of the ENFSI DNA working group is to establish
John M. Butler and Peter M. Vallone National Institute of Standards and Technology
DNA Biometrics: i Standards and Technology John M. Butler and Peter M. Vallone National Institute of Standards and Technology NDIA Biometrics Conference (Arlington VA) NDIA Biometrics Conference (Arlington,
Multiplex analysis of 10 STR loci plus Amelogenin
Multiplex analysis of 10 STR loci plus Amelogenin Page 1 of 15 Table of Content 1 Product Information.3 1.1 Product Description 3 1.2 Ordering Information and Kit Components 5 1.3 Storage Conditions..5
Biology 445K Winter 2007 DNA Fingerprinting
Biology 445K Winter 2007 DNA Fingerprinting For Friday 3/9 lab: in your lab notebook write out (in bullet style NOT paragraph style) the steps for BOTH the check cell DNA prep and the hair follicle DNA
Forensic DNA analysis
FORENSIC DNA ANALYSIS: A PRIMER FOR COURTS 1 Forensic DNA analysis A PRIMER FOR COURTS 2 FORENSIC DNA ANALYSIS: A PRIMER FOR COURTS Forensic DNA analysis: a primer for courts Issued: November 2017 DES4928
Design and Execution of Validation Studies for Establishing DNA Mixture Interpretation Procedures
DNA Mixture Interpretation Workshop Michael D. Coble, PhD Design and Execution of Validation Studies for Establishing DNA Mixture Interpretation Procedures Elements of DNA Mixture Interpretation Principles
Male/female DNA mixtures: a challenge for Y-STR analysis
International Congress Series 1239 (2003) 295 299 Male/female DNA mixtures: a challenge for Y-STR analysis Burkhard Berger, Harald Niederstätter, Silvano Köchl, Martin Steinlechner, Walther Parson * Institute
Comparison of the Promega Power Y and AB AmpFlSTR YFiler kits and the Subsequent Validation of YFiler.
Comparison of the Promega Power Y and AB AmpFlSTR YFiler kits and the Subsequent Validation of YFiler. Dr. Timothy P McMahon Armed Forces DNA Identification Laboratory Rockville, Md 301-319 319-0238 Timothy.mcmahon@afip.osd.mil
DNA: Biology & the Basics of DNA Typing
DNA: Biology & the Basics of DNA Typing Mary Dayton Assistant Public Defender Law Office of the DeKalb County Public Defender Stone Mountain Judicial Circuit 404.371.2222 mfdayton@dekalbcountyga.gov The
PowerPlex. Y System Validation
PowerPlex Y System Validation By Patricia M. Fulmer, Dawn Rabbach, Kimberly Huston, Curtis Knox and Cynthia Sprecher, Promega Corporation Abstract We have improved the manufacturing process for the PowerPlex
From Forensic Genetics to Genomics Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations
From Forensic Genetics to Genomics Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations Peter M. Schneider Institute of Legal Medicine University of Cologne
Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit
Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to
Forensic genetics: New frontiers
Forensic genetics: New frontiers Dennis McNevin (CRICOS) #00212K DNA profiles First application of DNA profiling : The Enderby murder case A single locus minisatellite probe was used to analyze the following
More often heard about on television dramas than on the news, DNA is the key to solving crimes the scientific way. Although it has only been
DNA Matching More often heard about on television dramas than on the news, DNA is the key to solving crimes the scientific way. Although it has only been relatively recent (compared the course of forensic
DNA Discovery and Deconstructing It
DNA Discovery and Deconstructing It Simon Ford, Ph.D. San Francisco Florida Forensic Science DNA Bootcamp Orlando February 24 th, 2017 What is all this stuff and what can I do with it? Paperwork: Paperwork
Biology/DNA DNA Interpretation SOP. Biology/DNA Division
Contents 1 Analysis and Interpretation of DNA Results... 3 2 Statistics... 51 3 Reports... 59 Document ID: 25414 Uncontrolled When Printed Page 2 of 61 1 Analysis and Interpretation of DNA Results Autosomal
PCR. What is PCR? What is PCR? Why chain? What is PCR? Why Polymerase?
What is PCR? PCR the swiss army knife Claudia Stäubert, Institute for biochemistry PCR is an exponentially progressing synthesis of the defined target DNA sequences in vitro. It was invented in 1983 by
Direct PCR Amplification of Blood Samples on Copan NUCLEIC-CARD TM Systems Using the AmpFlSTR Identifiler Direct PCR Amplification Kit
Technical Focus Direct PCR Amplification of Blood Samples on Copan NUCLEIC-CARD TM Systems Using the AmpFlSTR Identifiler Direct PCR Amplification Kit Nicola Oldroyd, Jennifer Schroeder, Dennis Wang, Alessandro
Mohammed Sanaullah Qaseem, Syed Abdul Wahab Asif, Zeeshan Fatima Armeen, Israr Ahmed Qureshi
.Advancement In DNA As Source Of Biometric Authentication Mohammed Sanaullah Qaseem, Syed Abdul Wahab Asif, Zeeshan Fatima Armeen, Israr Ahmed Qureshi Associate Professor, Nizam Institute of Engineering
The Real CSI: Using DNA to Identify Criminals and Missing Persons
The Real CSI: Using DNA to Identify Criminals and Missing Persons San Jose State University May 2, 2012 Overview Forensic DNA in the media perceptions and reality The power and limitations of nuclear (STR)
PowerPlex ESX & ESI Fast Systems
PowerPlex ESX & ESI Fast Systems PowerPlex ESX & ESI Fast Systems The PowerPlex ESX and ESI Fast Systems meet recommendations of the European Network of Forensic Science Institutes (ENFSI) for DNA profile
Analyzing Y-STR mixtures and calculating inclusion statistics
Analyzing Y-STR mixtures and calculating inclusion statistics Rick W. Staub, Ph.D. and Cassie Johnson, M.S. Orchid Cellmark Inc. Dallas, TX ABSTRACT Forensic evidence samples analyzed with Y-STR multiplexes
Application of Biotechnology in DNA Fingerprinting and Forensic Analysis. Copyright 2009 Pearson Education, Inc.
Application of Biotechnology in DNA Fingerprinting and Forensic Analysis Introduction to DNA Fingerprinting and Forensics Forensic science intersection of law and science Historic examples Early 1900s
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR Ref. PCR1 1. OBJECTIVE OF THE EXPERIMENT The objective of this experiment is to introduce students to the principles and practice of Polymerase Chain Reaction (PCR)