Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Microbiology Laboratory ltnagelvin Hospital Glenshane Road Londonderry BT47 6SP Northern Ireland Contact: Martin Robinson Tel: (028) ext: Direct dial: (028) Website: below Laboratory locations: s covered by the organisation and their relevant activities details ctivity code ddress Microbiology Laboratory ltnagelvin Hospital Glenshane Road Londonderry BT47 6SP Local contact Martin Robinson Microbiology Site activities performed away from the locations listed above: details ctivity code GUM Clinic nderson House ltnagelvin Hospital Gram staining Examination of wet prep slides B ssessment Manager: B2 Page 1 of 9
2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to DETIL OF CCREDITTION HUMN BODY FLUIDS ND TISSUES clinical diagnosis Whole Blood Isolation, identification and antibiotic susceptibility testing of bacterial isolates of clinical significance Using Biomerieux VirtuO Manual gram stain ntimicrobial susceptibility testing. Identification using oxidase, coagulase and streptococcal grouping tests. Confirmation of identification and susceptibility testing using Vitek MS, Vitek 2, and gradient sensitivity strips MIC/EXT/DOC/039 (VirtuO User Manual) MIC/IDENI/45 (gram stain) MIC/SENS/01 & MIC/SENS/03 (antimicrobial susceptibility testing) MIC/IDENI/63 (Vitek 2) MIC/IDENI/62 (MLDI-TOF Test Procedure) MIC/INV/01 (Inv of blood cultures) ssessment Manager: B2 Page 2 of 9
3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Swabs (xilla, Cervical, Ear, Eye, Groin, Mouth, Nasal, Neonatal screen, Peg tube, Penile swab, PVL screen, Rectal, Skin, Throat, Ulcer, Umbilical, Urethral, Vaginal, VRE/GRE screen, Vulval, Wound), Corneal scrapings, pus, abscess discharge, catheter tip, biliary stent, IUCD, tissue, faeces, CSF, peritoneal fluid, pleural fluid, ascetic fluid, synovial fluid, aqueous/vitreous humour Isolation, identification, and antibiotic susceptibility testing of bacteria of clinical significance Manual culture using prepared manufacturers agar plates including chromogenic media Manual gram stain ntimicrobial susceptibility testing. Identification using oxidase, coagulase and streptococcal grouping tests. Confirmation of identification and susceptibility testing using Vitek MS, Vitek 2, and gradient sensitivity strips MIC/IDENI/32 (Inoculation of Culture Media) MIC/IDENI/45 (gram stain) MIC/SENS/01 & MIC/SENS/03 (antimicrobial susceptibility testing) MIC/IDENI/63 (Vitek 2) MIC/IDENI/62 (MLDI-TOF Test Procedure) MIC/INV/20 (Inv of abscesses and post-surgical wounds) MIC/INV/05 (Inv of eye swabs) MIC/INV/04 (Inv of ear swabs) MIC/INV/17 (Inv of throat swabs) MIC/INV/21 (Inv of vaginal swabs) MIC/INV/11 (Inv of intravascular cannulae & ssoc specimens) MIC/INV/14 (Inv of nose swabs) MIC/INV/18 (Inv of tissues and biopsies) ssessment Manager: B2 Page 3 of 9
4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Swabs (xilla, Cervical, Ear, Eye, Groin, Mouth, Nasal, Neonatal screen, Peg tube, Penile swab, PVL screen, Rectal, Skin, Throat, Ulcer, Umbilical, Urethral, Vaginal, VRE/GRE screen, Vulval, Wound), Corneal scrapings, pus, abscess discharge, catheter tip, biliary stent, IUCD, tissue, faeces, CSF, peritoneal fluid, pleural fluid, ascetic fluid, synovial fluid, aqueous/vitreous humour (cont d) Isolation, identification, and antibiotic susceptibility testing of bacteria of clinical significance (cont d) MIC/INV/12 (Inv of intraocular fluids and corneal scapings) MIC/INV/02 (Inv of CSF) MIC/INV/08 (Inv of faeces for bacterial pathogens) MIC/INV/10 (Inv of fluids from sterile sites) MIC/INV/34 (Inv of Bile) MIC/INV/35 (Inv of mouth swabs) MIC/INV/36 (Inv of Superfical & Non-Surgical Wound Swabs) Swabs: Nasal, xilla, Groin, other body site, e.g. wound swab Isolation and identification of MRS bacteria Manual culture using prepared manufacturers chromogenic media agar plates. MIC/INV/16 (Investigation of specimens for Screening for MRS) Swabs include Nasal, Groin, xilla, other body site e.g wound swab Isolation and identification of Pseudomonas aeruginosa Manual culture using prepared manufacturers Pseudomonas selective agar. MIC/INV/31 (Investigation of specimens for Pseudomonas aeruginosa screening CSF, peritoneal fluid, pleural fluid, ascetic fluid, synovial fluid, aqueous/vitreous humour Detection and quantification of white blood cells, red blood cells and bacteria Manual microscopy using Mod- Fuchs Rosenthal haemocytometer MIC/IDENI/59 ssessment Manager: B2 Page 4 of 9
5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Urine (MSU, CSU, bag, bladder tap, suprapubic aspirate) Detection and quantification of red cells, white cells, epithelial cells, bacteria and yeasts. Culture, identification and sensitivity testing of significant bacteria. Manual microscopy using Kova slide. Manual culture using chromogenic agar. ntimicrobial susceptibility testing. Identification using oxidase, coagulase and streptococcal grouping tests. Confirmation of identification and susceptibility testing using Vitek MS, Vitek 2, and gradient sensitivity strips MSTDISCS ID for ESBL confirmation MIC/SENS/01 & MIC/SENS/03 (antimicrobial susceptibility testing) MIC/IDENI/63 (Vitek 2) MIC/IDENI/62 (MLDI-TOF Test Procedure) MIC/INV/19 (Inv of Urine) Urine, swabs (genital, throat, rectal, cervical, vaginal), smears (cervical, vaginal, urethral) Detection of bacteria of clinical significance for purposes of sexual health screen Manual gram stain Wet prep smears Light microscopy SOP numbers MIC/INV/22 (GUM clinic) MIC/IDENI/45 (gram stain) MIC/INV/21 (vaginal swabs) B ssessment Manager: B2 Page 5 of 9
6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Sputum, bronchoalveloar lavage, bronchial washing, pleural fluids, trap sputum, tracheal secretions, Respiratory samples for bacterial pathogens (not TB) Using manual mucolyse technique. Manual culture using prepared manufacturers agar plates Manual gram stain ntimicrobial susceptibility testing. Identification using oxidase, coagulase and streptococcal grouping tests. Confirmation of identification and susceptibility testing using Vitek MS, Vitek 2, and gradient sensitivity strips MIC/IDENI/45 (gram stain) MIC/INV/26 (Inv of Lower Respiratory Tract Infections) MIC/SENS/01 & MIC/SENS/03 (antimicrobial susceptibility testing) MIC/IDENI/63 (Vitek 2) MIC/IDENI/62 (MLDI-TOF Test Procedure) Swabs (HVS, mouth, ear, throat, swabs from immunocompromised patients), blood cultures, sterile fluids. Isolation and identification of yeasts of clinical significance. Please note: identification of yeasts to species level is carried out on isolates from blood cultures and sterile fluids only Manual culture using prepared manufacturers agar plates including chromogenic media, MIC/IDENI/32 (Inoculation of Culture Media) MIC/IDENI/64 64 (Wet prep procedure for yeasts) MIC/IDENI/65 (identification of spergillus species) ssessment Manager: B2 Page 6 of 9
7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) In-house documented methods using manufacturer s instructions for use Sputum, bronchoalveloar lavage, bronchial washing, pleural fluids, trap sputum, tracheal secretions, EBUS aspirate, pus, urine, gastric aspirate,blood, fluids, swabs, bone marrow, tissues and biopsies Isolation and presumptive identification of Mycobacterium species Using BD MGIT320 Ziehl Neelsen staining See also Molecular Examinations section MIC/INV/27 (Investigation of samples for Mycobacterium spp) Presumptive Mycobacterium species cultured in-house Detection of mycobacterial protein fraction MPT64 secreted from Mycobacterium tuberculosis complex (Mtbc) MGIT MTB identification kit MIC/INV/33 (BD MGIT TBc Identification Test) Faeces Detection of C. difficile toxin & B and glutamate dehydrogenase antigen lere Techlab C diff Quik Chek Complete enzyme immunoassay kit MIC/IDENI/39 Faeces Detection of: Rotavirus denovirus By lateral flow immunochromatographic methodology, using the Rota/denoscreen Cassette Test MIC/IDENI/40 ssessment Manager: B2 Page 7 of 9
8 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Serology examination clinical diagnosis In-house documented methods using manufacturer s instructions for use Urine Detection of Legionella pneumophila Serogroup 1 ntigens Meridian biosciences TRU Legionella rapid Immunoassay MIC/SER/17 Urine Detection of Streptococcus pneumoniae antigen Trinity Biotech Uni-Gold S. pneumonia rapid immunoassay MIC/SER/16 Nasal swab, nasal secretions Detection of respiratory syncytial virus (RSV) lere BINOX NOW immunechromatographic RSV card MIC/SER/10 Serum nti-streptolysin O Titre (SOT) BioKit Rheumajet SO kit MIC/SER/03 Serum Detection of total antibodies to Treponema pallidum BIOKIT Bioelisa syphilis 3.0 immuno-enzymatic assay (IgG & IgM) MIC/SER/02 ssessment Manager: B2 Page 8 of 9
9 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS ND TISSUES (cont d) Molecular examination clinical diagnosis In-house documented methods using manufacturer s instructions for use Endocervical swabs and urine Qualitative detection of Chlamydia trachomatis/neisseria gonorrhoeae Cobas 4800 CT/NG in vitro nucleic amplification test for qualitative detection of CT/NG MIC/SER/04 Endocervical swabs and urine Detection and differentiation of genomic DN from Chlamydia trachomatis/neisseria gonorrhoeae Cepheid Genexpert CT/NG PCR MIC/SER/22 Sputum and bronchoscopy samples Confimatory TB testing of positive ZN sample Cepheid Genexpert PCR SOP number MIC/INV/32 (Gene Xpert Mtb/Rif assay) END ssessment Manager: B2 Page 9 of 9
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