Tendon/Ligament Repair

Transcription

1 Tendon/Ligament Repair Team Collagen Constructs Mario Rossi, Anne Tucker, Brian Ziola Faculty Advisors: Dr. Yawen Li, Dr. Therese Bou-Akl Technical Advisors: Dr. Tristan Maerz, Meagan Salisbury

2 Problem Identification Meetings with Dr. Bou-Akl & Beaumont to determine clinically relevant needs Researched literature for needs and refinement Observed needs: Orthopedic need for tendon/ligament injury repair Cpubs.ePlatform.Service.FreeContent.I mageservice.svc/imageservice/articlei mage/2012/gc/c2gc35608b/c2gc3560 8b-f6.gif 2

3 Market Analysis Market Analysis: Orthopaedic soft tissue market size- $5B Growth: $9.39B by 2024 Stakeholder Analysis: Influential stakeholders: Patients, athletes, orthopaedic surgeons, insurance providers Decision makers: Surgeons and insurance providers 3

4 Current Solutions Non-surgical Surgical Icing Pharmaceuticals Physical therapy Sutures Grafts Limitations Non-surgical: Temporary, cannot repair complex tendon injuries Surgical: Does not distribute tensile load evenly, risk for immune rejection, and harvest site morbidity 4

5 Tissue Engineering Current studies use cells (usually stem cells) seeded on scaffold with or without mechanical stimulation Few stem cell-based tendon/ligament tissue engineering strategies translated to clinical trials Primarily due to: Significant cost Technical challenges Regulatory hurdles Time-intensive nature of autologous and allogeneic stem cell harvest 5

6 Need Statement There is a need for a similar biomechanical property, anatomically correct solution in tendon/ligament injury repair 6

7 Design Concept Absence of cells in design 7

8 Design Novelty / Design Parameters Novelty: Biomimetic bioactive scaffold Incorporation of alginate containing SDF-1 into scaffold to recruit MSCs Design Parameters: Biocompatibility of materials Biomechanical Properties Chemokine release Degradation rates 8

9 Hypothesis The created biocompatible collagen scaffold will be have similar biomechanical properties as native tendons/ligaments. Also, the scaffold will recruit stem cells and promote growth with the incorporation of SDF-1 9

10 Estimated Deliverables Scaffold that has similar biomechanical properties to that of anatomical tendons Scaffold recruits MSCs in vitro Scaffold promotes cell growth Can be implanted in lieu of grafting 10

11 Methods Test and Experiments to be done: Collagen fiber spinning and scaffold assembly Scaffold characterization Biocompatibility testing Mechanical testing Degradation study Release study Equipment to be used: MTS machine Cell culture equipment Laboratory equipment Pipettes, hoods, centrifuge, vortexer Confocal Microscope Micro CT/ESEM 11

12 Design Validation Scaffold characterization: Use ESEM and uct to characterize scaffold microstructure Biocompatibility testing: Evaluate cell attachment and proliferation using alamar blue assay and confocal fluorescence microscopy Mechanical testing: Compare biomechanical properties with those reported in literature 12

13 Design Validation Release study: Determine SDF-1 release profile, burst release desired Degradation study: Determine the rate at which the collagen structure breaks down MSC migration study: Use boyden chamber assay to assess the MSC migration in close proximity to the collagen scaffold containing SDF-1 13

14 Budget Leftover LESA funding from previous years work Beaumont and Providence providing free access to equipment 14

15 Timeline 15

16 Team Structure 16

17 References By Procedure (Rotator Cuff Repair, Epicondylitis, Achilles Tendinosis Repair, Pelvic Organ Prolapsed, Gluteal Tendon, Cruciate Ligaments Repair, Hip Arthroscopy, Biceps Tenodesis), By Injury Location (Knee, Shoulder, Hip, Small Joints), And Trend Analysis From 2013 To "Orthopedic Soft Tissue Repair Market Size, Share Report, 2024." Orthopedic Soft Tissue Repair Market Size, Share Report, N.p., Oct Web. 27 Oct Shearn, Jason T. et al. Tendon Tissue Engineering: Progress, Challenges, and Translation to the Clinic. Journal of musculoskeletal & neuronal interactions 11.2 (2011): Print. "Research and Markets Adds Report: Orthopedic Soft Tissue Repair Market - Global Industry Analysis, Size, Share, Growth, Trends and Forecast, " PRNewswire. N.p., 25 Mar Web. 27 Oct Kirkendall, DT and Garrett, WE. Function and Biomechanics of Tendons. Scandinavian Journal of Medicine and Science in Sports. 2(1997): Web. 24 Oct Tendon. Encyclopaedia Britannica. Encyclopaedia Britannica, Inc. 2 Feb Web. 25 Oct Renstrom, Per A.F.H. and Lynch, Scott A. Ankle Ligament Injuries. Brazilian Journal of Sports Medicine. 4.3 (1998). Web. 27 Oct Erickson, Brandon J. et al. Trends in the Management of Achilles Tendon Ruptures in the United States Medicare Population, Orthopedic Journal of Sports Medicine. 2.9 (2014). Web. 27 Oct Schulze-Tanzil, Gundala et al. Decellularized Tendon Extracellular Matrix - A Valuable Approach for Tendon Reconstruction? Cells 1.4 (2012): PMC Web. 7 Nov Noyes, Frank R and Grood, Edward S. The Strength of the Anterior Cruciate Ligament in Humans and Rhesus Monkeys J of Bone and Joint Surgery. (1977): 58: ResearchGate Web. 1 Dec

18 Questions? 18