BioXtra Over 75 essential offers and exciting new products

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Clemence Andrews
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1 2015 Over 75 essential offers and exciting new products

Laboratory essentials Sample filtration Whatman syringe filters Syringe filters combining premium quality and economy For quick and efficient filtration of samples and buffers up to 100 ml in volume.

2 Laboratory essentials Sample filtration Whatman syringe filters Syringe filters combining premium quality and economy For quick and efficient filtration of samples and buffers up to 100 ml in volume. Sonically welded to minimize interference with sample. Whatman GD/X with glass microfiber prefilters that have high particulate loading capacity. GD/XP for samples that require inorganic ion analysis. ordering code Total list price price GD/XP 25 mm Syringe Filter, 0.45 μm, nylon, 150/pk 1 pack P $ $ GD/XP 25 mm Syringe Filter, 0.45 μm, PVDF, 150/pk 1 pack P $ $ GD/XP 25 mm Syringe Filter, 0.45 μm, PTFE, 150/pk 1 pack P $ $ GD/XP 25 mm Syringe Filter, 0.45 μm, PP, 150/pk 1 pack P $ $ GD/XP 25 mm Syringe Filter, 0.45 μm, PES, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, nylon, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, PVDF, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, PTFE, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, PTFE, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, PES, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, PES, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, PP, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, PP, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, cellulose acetate, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, cellulose acetate, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.2 μm, regenerated cellulose, nonsterile, 150/pk 1 pack P $ $ GD/X 25 mm Syringe Filter, 0.45 μm, regenerated cellulose, nonsterile, 150/pk 1 pack P $ $

Save up to 30% Sample filtration Whatman Mini-UniPrep products Sample filtration for HPLC/uHPLC analysis Replaces syringe, syringe filter, vial, and cap.

3 Save up to 30% Sample filtration Whatman Mini-UniPrep products Sample filtration for HPLC/uHPLC analysis Replaces syringe, syringe filter, vial, and cap. Load directly in to the autosampler (compatible with any autosampler that accommodates standard 12 mm 32 mm profile vials). Available in plastic (Mini-UniPrep with blue cap) or borosilicate glass (Mini-UniPrep G2** with purple cap). Process multiple samples at the same time (6 or 8) saving time. Minimizes instrument downtime due to unfiltered samples. Reduces laboratory waste. ordering code Total list price price Mini-UniPrep (plastic construction) Mini-UniPrep Clear, Slit Septum Cap, 0.2 μm, Nylon, 100/pk 1 pack P $ $ Mini-UniPrep Clear, Slit Septum Cap, 0.2 μm, PTFE, 100/pk 1 pack P $ $ Mini-UniPrep Clear, Standard Cap, 0.2 μm, Nylon, 100/pk 1 pack P $ $ Mini-UniPrep Clear, Standard Cap, 0.45 μm, Nylon, 100/pk 1 pack P $ $ Mini-UniPrep Clear, Standard Cap, 0.45 μm, PTFE, 100/pk 1 pack P $ $ Mini-UniPrep, Amber Housing, Standard Cap, 0.45 μm, PTFE, 100/pk 1 pack P $ $ Mini-UniPrep, Amber Housing, Standard Cap, 0.2 μm, Nylon, 100/pk 1 pack P $ $ Mini-UniPrep, Amber Housing, Standard Cap, 0.2 μm, PTFE, 100/pk 1 pack P $ $ Mini-UniPrep, Amber Housing, Standard Cap, 0.45 μm, Nylon, 100/pk 1 pack P $ $ Mini-UniPrep G2 (glass construction) Mini-UniPrep G2 Clear, Standard Cap, 0.45 μm, RC, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.45 μm, Glass Fiber, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.2 μm, RC, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.2 μm, PTFE, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.45 μm, PTFE, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.2 μm, PVDF, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.45 μm, PVDF, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.2 μm, Nylon, 100/pk + HC 1 pack P $ $ Mini-UniPrep G2 Clear, Standard Cap, 0.2 μm, PP, 100/pk + HC 1 pack P $ $ HC - Hand compressor 3

Save up to 40% Cell isolation Ficoll-Paque and Percoll density gradient media Ready-to-use medium for isolation of human lymphocytes with high yield from peripheral blood Maintains viability and

Surface protectors Benchkote laboratory surface protectors Highly absorbent, impermeable material designed to protect laboratory surfaces against hazardous spills.

4 Save up to 40% Cell isolation Ficoll-Paque and Percoll density gradient media Ready-to-use medium for isolation of human lymphocytes with high yield from peripheral blood Maintains viability and representative distribution of B and T lymphocytes. Low levels of endotoxin (< 0.12 EU/ml). Surface protectors Benchkote laboratory surface protectors Highly absorbent, impermeable material designed to protect laboratory surfaces against hazardous spills. High-quality, smooth, absorbent Whatman paper. Benchkote Plus is capable of absorbing up to 0.75 liters of water per square meter. Smooth white surface can be written on with ink or pencil and lies flat. ordering code Total list price price Percoll 1 L Density Gradient Media 1 Bottle P $ $ Ficoll-Paque Plus ml Density Gradient Media 1 pack P $ $ Benchkote Plus Surface Protector, 500 x 600 mm, 50 sheets/pk 1 pack P $ $ Benchkote Surface Protector, mm, 50 sheets/pk 1 pack P $ $ Benchkote Surface Protector, 460 mm 50 m, 1 reel/pk 1 pack P $ $

Measure your samples Spectrophotometers SimpliNano spectrophotometer Simple-to-use microvolume spectrophotometer

Conserve precious samples by measuring volumes as low as 1 to 5 μl.

Conserve precious samples by assaying low volumes from 0.5 to 5 μl.

5 Measure your samples Spectrophotometers SimpliNano spectrophotometer Simple-to-use microvolume spectrophotometer Measurement of nucleic acids and proteins. Reliable and easy-to-use with no moving parts. Conserve precious samples by measuring volumes as low as 1 to 5 μl. NanoVue Plus spectrophotometer Low volumes made easy Eliminate the need for cuvettes, capillaries or other sample devices. Conserve precious samples by assaying low volumes from 0.5 to 5 μl. ordering code Total list price price SimpliNano Spectrophotometer 1 unit P $6, $5, SimpliNano Spectrophotometer with Printer 1 unit P $6, $5, NanoVue Plus Spectrophotometer with Printer 1 unit P $11, $9,

Nucleic acids Stock up now: up to 30% off Purification illustra GFX PCR* DNA and Gel Band Purification Kit Isolation and concentration of DNA fragments from PCR mixtures, agarose gel bands, and

Sample preparation illustra RTG GenomiPhi DNA amplification kit Kit offers a highly efficient and representative amplification of the whole genome. Shipping and storage at room temperature.

6 Nucleic acids Stock up now: up to 30% off Purification illustra GFX PCR* DNA and Gel Band Purification Kit Isolation and concentration of DNA fragments from PCR mixtures, agarose gel bands, and enzyme digests in under 10 min. Flexible elution volume for different DNA concentrations. DNA for use in sequencing, PCR, labeling, digestion, and cloning. Sample preparation illustra RTG GenomiPhi DNA amplification kit Kit offers a highly efficient and representative amplification of the whole genome. Shipping and storage at room temperature. 12 to 20 μg yield from nanogram amounts of DNA sample. ordering code Total list price price illustra RTG PCR*, 0.2 ml tube, hinged cap, 96 reactions 1 pack P $ $ illustra RTG PCR, 0.2 ml plate, 96 reactions 1 pack P $ $ illustra GFX PCR DNA and Gel Band Purification Kit, 100 purifications 1 pack P $252,00 $

Protein purification Save up to 20% Desalting and buffer exchange PD-10 columns Rapid and simple desalting, buffer exchange, and removal of low molecular weight compounds.

Columns HiTrap protein purification columns Fast, simple, and convenient purification. Can be used manually with a syringe, or with a pump or chromatography system including ÄKTA start.

40 HiPrep 26/10 Desalting columns 1 pack 95055-814P $726.00 $653.40 HiTrap Protein G HP, 1 5 ml column 1 pack 95055-848P $726.00 $653.40 HiTrap Protein G HP, 5 1 ml column 1 pack 95055-944P $848.

7 Protein purification Save up to 20% Desalting and buffer exchange PD-10 columns Rapid and simple desalting, buffer exchange, and removal of low molecular weight compounds. Disposable PD-10 desalting columns offer a simple alternative to dialysis. HiTrap desalting requires less than 1 min for buffer exchange and desalting. Columns HiTrap protein purification columns Fast, simple, and convenient purification. Can be used manually with a syringe, or with a pump or chromatography system including ÄKTA start. Scale-up is easy by connecting columns in series. ordering code Total list price price PD-10 Desalting columns, disposable, 30/pk 1 pack P $ $ HiPrep 26/10 Desalting columns 1 pack P $ $ HiTrap Protein G HP, 1 5 ml column 1 pack P $ $ HiTrap Protein G HP, 5 1 ml column 1 pack P $ $ PD SpinTrap G-25 column 1 pack P $ $ HiTrap Desalting, 5 5 ml column 1 pack P $ $ HiTrap rprotein A FF, 1 x 5 ml column 1 pack P $ $ HiTrap Protein A HP, 5 1 ml column 1 pack P $ $ HiTrap rprotein A FF, 5 x 1 ml column 1 pack P $ $ HiTrap Protein A HP, 1 5 ml column 1 pack P $ $ PD MultiTrap G-25, 4/pk 1 pack P $ $ illustra NAP-10 column, 50 columns/pk 1 pack P $ $

Western blotting Protein electrophoresis Amersham ECL Gel for protein gel electrophoresis Electrophoresis mini-gels with long shelf life of up to 12 mo Robust gels that allow resolution of highly

Gel electrophoresis Rainbow Molecular Weight Markers Allow you to achieve sharper, more intense bands on gels and blots plus more accurate molecular weight determination Comprised of 10 individually

8 Western blotting Protein electrophoresis Amersham ECL Gel for protein gel electrophoresis Electrophoresis mini-gels with long shelf life of up to 12 mo Robust gels that allow resolution of highly complex protein samples and ensure high electrotransfer efficiency for Western blotting. Everything required to access and cut the gel is included in the Amersham ECL Gel cassette. Gel electrophoresis Rainbow Molecular Weight Markers Allow you to achieve sharper, more intense bands on gels and blots plus more accurate molecular weight determination Comprised of 10 individually colored proteins in the molecular weight range of to Produces 6 different color bands of equal intensity. ordering code Total list price for bundle price 10 Amersham ECL Gel, 12%, 10 wells, up to 12 months shelf life 3 packs P $ $ Amersham ECL Gel, 4-12%, 10 wells, up to 12 months shelf life 3 packs P $ $ Full Range Molecular Weight Marker Rainbow (Mr to ), 250 μl 2 packs P $ $

30% more for your money on rolls Blotting Nitrocellulose (NC) blotting membranes Amersham Protran membranes offer the advantage of low background in Western blotting regardless of detection method

Exceptional handling characteristics of Amersham Protran supported NC membranes allows for stripping and reprobing of the membrane.

Suitable for Western blotting applications with stripping and reprobing. ordering code Total list price for bundle price Amersham Protran Premium 0.

9 30% more for your money on rolls Blotting Nitrocellulose (NC) blotting membranes Amersham Protran membranes offer the advantage of low background in Western blotting regardless of detection method used. Well-suited for applications where X-ray film is used for detection. Exceptional handling characteristics of Amersham Protran supported NC membranes allows for stripping and reprobing of the membrane. Polyvinylidene difluoride (PVDF) blotting membranes Western blotting membranes with greater mechanical strength than NC membranes. Suitable for Western blotting applications with stripping and reprobing. ordering code Total list price for bundle price Amersham Protran Premium 0.2 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran 0.2 μm NC 200 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran Supported 0.45 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran 0.45 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran Premium 0.45 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran Supported 0.45 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran Premium 0.45 μm NC 80 mm 90 mm, 25 sheets/pk 2 packs P $ $ Amersham Hybond 0.45 μm PVDF 254 mm 4 m, 1 roll/pk 2 packs P $ $ Amersham Protran Premium 0.45 μm NC 200 mm 200 mm, 10/pk 2 packs P $ $ Amersham Protran 0.2 μm NC 300 mm 4 m, 1 roll/pk 2 packs P $ $

Longer shelf life: easier storage! Detection Amersham ECL, ECL Select and ECL Prime Western blotting detection reagents High sensitivity reagent for chemiluminescent Western blotting detection.

Amersham ECL Prime: Suitable for detection of low abundance proteins.

Publication-quality images. ordering code Total list price for bundle price Rabbit IgG HRP-Linked Whole Antibody from Donkey, 1 ml 2 packs 95017-556P $618.

10 Longer shelf life: easier storage! Detection Amersham ECL, ECL Select and ECL Prime Western blotting detection reagents High sensitivity reagent for chemiluminescent Western blotting detection. Amersham ECL: Reagent of choice for confirmatory Western blotting applications. Amersham ECL Select: High sensitivity and broad linear dynamic range enables qualitative and quantitative anayses. Amersham ECL Prime: Suitable for detection of low abundance proteins. Imaging Amersham Hyperfilm ECL detection film Sensitive film for the detection of chemiluminescent signals in Western blotting assays. Clear background for excellent contrast and band visibility. Publication-quality images. ordering code Total list price for bundle price Rabbit IgG HRP-Linked Whole Antibody from Donkey, 1 ml 2 packs P $ $432,60 Mouse IgG HRP-Linked Whole Antibody from Sheep, 1 ml 2 packs P $ $ ECL Western Blotting Detection Reagents for 2000 cm 2 2 packs P $ $ ECL Western Blotting Detection Reagents for 4000 cm 2 2 packs P $ $ ECL Prime Western Blotting Detection Reagent for 1000 cm 2 2 packs P $ $ ECL Select Western Blotting Detection Reagent for 1000 cm 2 2 packs P $ $ Hyperfilm ECL cm, 50 sheets/pk 3 packs P $ $ Hyperfilm ECL 5 7 inches, 50 sheets/pk 3 packs P $ $

11 Selection guides Protran Optitran Westran S Hybond GE Healthcare Life Sciences offers a broad selection of nitrocellulose (NC) and polyvinylidine difluoride (PVDF) Western blotting membranes, with pore sizes ranging from 0.1 to 0.45 μm to suit your application requirements. By bringing together the trusted Hybond and Whatman brands, Amersham Western blotting membranes offer sustained high quality and performance. Amersham Western blotting membranes Two great brands join forces Nitrocellulose membranes New names Whatman Protran BA85, BA83 and BA79 Amersham Protran NC Amersham Hybond ECL Amersham Protran NC Premium Whatman Optitran BAS85 and BAS83 Amersham Protran NC Supported PVDF membranes New names Amersham Hybond P 0.2 PVDF Amersham Hybond P and Whatman Westran CS Amersham Hybond P 0.45 PVDF Amersham Hybond LFP Amersham Hybond LFP 0.2 PVDF Whatman Westran S Amersham Hybond SEQ 0.2 PVDF NEW ECL reagents selection guide A comparison table outlining the differences between ECL reagents. Amersham ECL Amersham ECL Prime Amersham ECL Select Amersham ECL Plex Description For routine detection and confirmatory analysis of high to medium protein levels For quantitative and confirmatory analysis of high to low protein levels For quantitative and confirmatory analysis of medium to very low protein levels For multiplex protein analysis and quantitation of high to very low protein levels Detection type Chemiluminescence Chemiluminescence Chemiluminescence Fluorescence Optimal membrane Amersham Protran Premium Amersham Hybond-P Amersham Protran Premium Amersham Hybond-P Amersham Protran Premium Amersham Hybond-LFP Amersham Protran Premium Recommended detection Amersham Hyperfilm (X-ray film) or ImageQuant LAS series ImageQuant LAS series X-ray film is also an option ImageQuant LAS series X-ray film is also an option Typhoon FLA series ImageQuant LAS 4010 Signal duration > 2 h > 24 h > 2 h > 3 mo Typical applications Routine protein detection Protein confirmation Overexpressed proteins Tagged proteins Protein quantitation Protein confirmation Protein regulation and expression analysis Protein quantitation Protein confirmation Protein regulation and expression analysis Multiplex analysis Normalization to housekeeping protein Analysis of posttranslational modifications and isoforms Typical results 5 μg 40 ng 5 μg 20 ng 1.2 μg 5 ng 10 μg 5 ng WB ERK ½; LOD: 40 ng; DR: 2.1 WB ERK ½; LOD: 20 ng; DR: 2.4 WB ERK ½; LOD: 5 ng; DR: 2.4 WB ERK ½ (Cy5 on FLA 9500); LOD 10 ng; DR: 3 11

Selected handbooks GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and

GE handbooks may be downloaded from www.gelifesciences.com/handbooks. The VWR All-You-Need for Proteomics brochure may be downloaded from www.vwr.com/all-you-need-proteomics.

Protein sample preparation handbook (28-9887-41) This 123-page handbook, intended for students and experienced practitioners alike, provides background and important considerations in the preparation

12 Selected handbooks GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. GE handbooks may be downloaded from The VWR All-You-Need for Proteomics brochure may be downloaded from Please contact your local VWR representative to obtain hard copy versions. Protein sample preparation handbook ( ) This 123-page handbook, intended for students and experienced practitioners alike, provides background and important considerations in the preparation of protein samples for further analysis. Western blotting principles and methods handbook ( ) The aim of this 192-page handbook is to guide and inspire beginners as well as experts toward successful Western blotting. It provides expertise and support, taking you through the complete Western blotting workflow, from sample preparation to detection and analysis. VWR All You Need for Proteomics For an overview of the VWR proteomics portfolio, this brochure includes products covering the complete workflow, whatever your needs. GE Healthcare reserves the right subject to any regulatory approval if required, to make changes in specifications and features shown herein, or discontinue the product described at any time without notice or obligation General Electric Company All rights reserved. First published January 2015 GE and GE monogram are trademarks of General Electric Company. ÄKTA, Amersham, Benchkote,, ECL, ECL Plex, ECL Select, Ficoll-Paque, GenomiPhi, GFX, HiPrep, HiTrap, Hyperfilm, illustra, ImageQuant, Mini-UniPrep, MultiTrap, NanoVue, Percoll, Protran, RTG, Rainbow, Simplinano, SpinTrap, Typhoon, Whatman, and Whatman GD/X are trademarks of General Electric Company or one of its subsidiaries. VWR is a registered trademark of VWR International, LLC. All other third party trademarks are the property of their respective owner. Ready-To-Go RT-PCR Beads. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, and 6,127,155. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche.om Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Every effort will be made to give reasonable notice of price changes but we reserve the right to change prices without further notice. All prices exclude value added or sales tax and may be subject to change without notice. These offers cannot be used in conjunction with any previously arranged purchase agreement. * The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F Hoffman-La Roche Ltd. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such as GE Healthcare and affiliates when used in conjunction with an authorised thermal cycler. All offers in this issue are valid until Expiration date June, 30, All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information M Lit. No CN.CA Promo Code 4353

Protein Xtra 2018 Protein Workflow Simplified

Protein Xtra 2018 Protein Workflow Simplified Laboratory Essentials Page 11 SAVE 15% Isolation & Cloning SAVE 10% Page 2 up to 15% Protein Purification Page 6 Sample Preparation SAVE up to 25% Page 3 SAVE