Thermo Scientific Nucleic Acid Technologies

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Emmeline Gaines
5 years ago
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1 Thermo Scientific Nucleic Acid Technologies Phosphoramidites for Oligonucleotide Synthesis High Purity Nucleotides and Polynucleotides Fluorescent Labeling and Detection Custom Chemistries Molecular Biology and Diagnostic Reagents Custom Bioprocessing

About Thermo Scientific Genomics The analysis of gene expression and regulation and technologies used to study the effects of altering gene expression are central to the science of genomics.

We develop and supply innovative technologies that enable rapid advancement of the understanding of genes, their expression, regulation and interactions in complex biological pathways.

2 About Thermo Scientific Genomics The analysis of gene expression and regulation and technologies used to study the effects of altering gene expression are central to the science of genomics. These technologies are employed in discovery research, molecular diagnostics and the development of new RNA- and DNA-based therapeutics. We develop and supply innovative technologies that enable rapid advancement of the understanding of genes, their expression, regulation and interactions in complex biological pathways. The comprehensive Thermo Scientific genomics offering provides innovative solutions for the life sciences and drug discovery markets. Our growing portfolio includes: Nucleic Acid Technologies ABgene PCR Reagents and Plastic Consumables Dharmacon RNAi Technologies Open Biosystems RNAi and Genomics Technologies

3 Thermo Scientific Nucleic Acid Technologies All the Essentials, Every Step of the Way We have served the nucleic acid chemistry needs of research scientists, nucleic acid diagnostic and therapeutic manufacturers for more than 50 years. The unmatched breadth and depth of our expertise enables us to meet or exceed the rigorous demands for quality and purity of therapeutic and diagnostic manufacturers. We provide premium DNA and RNA synthesis chemistries, molecular biology reagents and biomanufacturing that enable the development of innovative research and diagnostic tools and therapeutic compounds. Our customers include the leading innovators in the areas of: Molecular diagnostics RNAi therapeutics and research Gene expression analysis Next-generation sequencing technologies We have all the essentials to ensure that your development and manufacturing projects are a success: Industry leading custom development and manufacturing services, including analytical capabilities. A broad offering including Thermo Scientific PurePeak nucleotides, TheraPure phosphoramidites and DyLight nucleic acid labeling reagents. Quality systems to respond to your evolving requirements with rigorous supply chain, manufacturing process and quality control documentation and process change control. Dedicated account representatives to provide you with world class customer service.

Phosphoramidites for Oligonucleotide Synthesis As the global leader in high quality oligonucleotide synthesis reagents, we bring you unparalleled expertise in: Protection chemistries Custom

4 Phosphoramidites for Oligonucleotide Synthesis As the global leader in high quality oligonucleotide synthesis reagents, we bring you unparalleled expertise in: Protection chemistries Custom development Manufacturing scale up Innovative manufacturing process development You can rely on us at every stage of your project from small batches of materials for evaluation purposes, through full scale manufacturing. Our supply chain controls ensure that the raw materials that enter our processes are consistent from batch to batch. State of the art analytical capabilities provide the assurance that your critical product specifications are being met.

Custom Development and Manufacturing Exceptional Capabilities at Any Scale Clients in diagnostics, therapeutics and basic research value our expertise in new product development and manufacturing

5 Custom Development and Manufacturing Exceptional Capabilities at Any Scale Clients in diagnostics, therapeutics and basic research value our expertise in new product development and manufacturing process optimization. Communication is the key, from important areas of your business to corresponding functions in ours. In addition to development and synthesis, we offer a full range of processing, dispensing, packaging and labeling services. We develop integrative partnerships with our customers, providing the highest quality products and tailored support services from concept through manufacturing scale-up. A thorough understanding of your requirements is fundamental to how we do business. Thermo Scientific TheraPure RNA Phosphoramidites Quality and Purity Essential to Oligonucleotide Therapeutics Strictly controlled manufacturing processes limit the levels of reactive impurities and reduce the risk of undesirable reactions during synthesis Flexible manufacturing that can accommodate any batch size Purity standards anticipating the requirements for batches used in clinical trials Typical NMR profile for TheraPure DNA phosphorandites. Scale expansion reveals no contaminants in critical impurity range.

All Biology Needs a Little Chemistry We have pioneered the manufacturing of deoxy-, dideoxy- and ribonucleotides and molecular biology reagents.

6 All Biology Needs a Little Chemistry We have pioneered the manufacturing of deoxy-, dideoxy- and ribonucleotides and molecular biology reagents. Our manufacturing methods have kept pace with the ever increasing purity required by highly sensitive applications and emerging technologies. Whether you are developing kits for research or clinical diagnostic use our secure supply chain gives you the assurance that we will be able to meet your requirements consistently from batch to batch. In addition to standard products we offer custom production services for nucleotide analogs, synthetic oligo- and polynucleotides, alpha-thiol nucleotides, and RNA cap analogs. Thermo Scientific PurePeak Nucleotides Superior Performance in the Most Demanding Assays Extensive purification and functional testing assures optimal results in challenging applications including qpcr, RT-PCR sequencing and long amplifications. As one of the few original manufacturers of ultra-pure nucleotides in the world, we maintain rigorous control over the supply of critical raw materials, ensuring consistently higher quality product. We have the scale-up capacity, process controls and quality systems to meet the demands of companies manufacturing kits for both life science laboratory research and molecular diagnostics. HPLC demonstrates >99% Purity of PurePeak dtnps Absorbance (mau) Elution Time (min)

Thermo Scientific DyLight Fluorescent Technology Bright Alternatives for Nucleic Acid Detection Freedom from licensing requirements and royalty fees improves the

Bring us your most challenging assay and labeling requirements - our R&D team has extensive expertise developing and optimizing chemistries to meet the needs of the most

Our offering includes labeled phosphoramidites (for chemical oligonucleotide synthesis), nucleoside triphosphates (for enzymatic labeling) and custom-designed products.

7 Thermo Scientific DyLight Fluorescent Technology Bright Alternatives for Nucleic Acid Detection Freedom from licensing requirements and royalty fees improves the economics of your new products. Bring us your most challenging assay and labeling requirements - our R&D team has extensive expertise developing and optimizing chemistries to meet the needs of the most sensitive applications. Our offering includes labeled phosphoramidites (for chemical oligonucleotide synthesis), nucleoside triphosphates (for enzymatic labeling) and custom-designed products. Spectral characteristics and performance are comparable to Cy and Alexa Fluor dyes. Sequence-specific staining of nuclei and chromosomes with DyLight labeled oligonucleotides Photographs courtesy of One Cell Systems Excitation and Emission Spectra of DyLight (DY547) & Cy3 Labeled Oligos Cy3 oligo (excitation) Cy3 oligo (emission) Dy547 oligo (excitation) Dy547 oligo (emission) Fluorescence Intensity Wavelength (nm)

Molecular Biology Reagents and Bioprocessing Our rich tradition in the manufacture of molecular biology products includes Taq polymerase, RNase inhibitors, DNA and RNA polymerases and Coenzyme A and

8 Molecular Biology Reagents and Bioprocessing Our rich tradition in the manufacture of molecular biology products includes Taq polymerase, RNase inhibitors, DNA and RNA polymerases and Coenzyme A and its derivatives. State of the art manufacturing and separation technologies allow us to meet the most exacting requirements for product purity and biological activity. In addition to proteins, we can supply in liquid or dry form: DNA and RNA from bacterial viral, mammalian and other sources purified to your specifications Molecular weight markers and ladders in increments of your choice Digested DNA fragments We offer a full suite of product finishing services that can handle quantities from milliliters to thousands of liters. These include automated fill and transfer, light, moisture resistant and tamper evident packaging, lyophilization, and custom label formatting and bar-coding. Component testing capabilities include NMR, mass spectrometry and enzymatic analysis.

9 The quality you demand. No surprises. Balanced Control and Flexibility Our quality, consistency, and security are critical in your supply chain. We understand that the quality of our suppliers and raw materials is critical to the consistent performance of your products. Quality systems are the cornerstone of our business and include: A formalized change control process to ensure consistency, compliance and conformance of materials. Raw material and vendor qualifications that provide security of supply. Corrective and preventative action systems to address potential customer issues. Rigorous standards of product testing and process controls that include assay development and validation, stability studies, equipment qualification and process verification and validation. ISO registration. Our employees use these systems in their day-to-day activities and take great pride in consistently meeting our customers' requirements and exceeding their expectations for service. We encourage customers to tour our facility. Please contact us at or mke.csm@thermofisher.com if you would like to arrange a visit.

10 Customer Service Customer satisfaction drives our business decisions and daily work flow. Because long-term relationships with our customers are our goal, we focus on delivering the standard of service that only a highly experienced staff can provide. We re committed to communication, are responsive and deliver on our promises. Your account will be assigned a dedicated representative with years of experience across a variety of job functions in our organization. When you call our Customer Service team, you will have direct contact with a representative not an automated system. We provide prompt, accurate responses to questions, documentation requests, and technical inquiries. > 98% on-time delivery performance. Stock items can typically be delivered in 48 hours. Customer Service 2200 N. Bartlett Avenue Milwaukee, WI Phone: Toll Free: FAX: mke.csm@thermofisher.com

About Thermo Fisher Scientific Thermo Fisher Scientific is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer.

universities, research institutions and government agencies, as well as environmental and industrial process control settings.

11 About Thermo Fisher Scientific Thermo Fisher Scientific is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of highend analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike.

12 2008 Thermo Fisher Scientific Inc. All rights reserved. Cy is a trademark of GE Healthcare. Alexa Fluor is a trademark of Invitrogen. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Literature # MK0030E01 Life Science Research 2202 North Bartlett Avenue Milwaukee, WI (877) toll-free (414) fax

Start-Up and Emerging Companies Program. High quality nucleic acid products for research, diagnostics, and therapeutics

Start-Up and Emerging Companies Program High quality nucleic acid products for research, diagnostics, and therapeutics Personalized Support When you partner with TriLink as your trusted supplier, you ll