IMPLEMENTATION OF PANORAMA INTO DAILY WORKFLOWS AND QUANTITATIVE PROTEIN PK ANALYSIS IN THE PHARMACEUTICAL SETTING
|
|
- Deborah Newton
- 5 years ago
- Views:
Transcription
1 IMPLEMENTATION OF PANORAMA INTO DAILY WORKFLOWS AND QUANTITATIVE PROTEIN PK ANALYSIS IN THE PHARMACEUTICAL SETTING Kristin Geddes Associate Principal Scientist Pharmacokinetics, Pharmacodynamics and Drug Metabolism Discovery Bioanalytics, West Point, PA Skyline Users Group Meeting, San Diego, CA June 3, 2018
2 Outline 1. Overview of workflows and instrumentation 2. Skyline and data processing 3. Panorama AutoQC Standard Curves PK Calculations 2
3 RT: Time (min) LC-MS Protein PK Assay Development NL: 1.58E7 Base Peak MS Relative Abundance Nanoacquity Orbi Velos Mapping Peptide selection CE Opt Matrix Check 10 min Methods Agilent 6495 QQQ Target Assay Development/Refinement Sample Prep: ImmunoCapture In-solution Digestion Tissue Homogenization Digestion Optimization Standard curves Automation Sample Preparation 3
4 Thermo Orbitrap Velos Foundational Analytical Platforms Thermo QE-HF Waters Xevo-TQS QQQ Discovery and mapping Agilent 6495 QQQ Discovery, mapping and PRM experiments Thermo TSQ Vantage QQQ High throughput peptide/protein quantitation AB Sciex 6500 Q-Trap High throughput peptide/protein quantitation Quantitation of biomarkers and endogenous proteins High throughput protein/peptide quantitation 4
5 Skyline: The Foundational Software Tool With the wide variety of instrumentation in our laboratories, data analysis using Skyline is critical. Method building and refinement CE Optimization Spectral Libraries Calibration Curves Peak Areas Retention times 5
6 From Skyline Data to Reports for Collaborators SKYLINE Excel PKLIMS reporting tool Watson LIMS 6
7 How do we efficiently go from Skyline to a PK report? 7
8 Panorama and P3 Panorama is a freely-available, open-source repository server application for targeted mass spectrometry assays that integrates into a Skyline mass spec workflow. It has been implemented as a module within LabKey Server, an open-source bioinformatics data management platform with extensive support for proteomics and small molecule data and a security model rich enough to support clinical studies AutoQC uploader Organize projects across the group Allows for multiple projects to be set up on the server 8
9 Tracking Instrument and Assay Performance in Panorama: AutoQC We use instrument QC s for things such as instrument life-cycle management and knowing the instrument status before starting a run. AutoQC is great because it automatically sweeps system suitability files up to Panorama and adds the replicates to a Skyline document Allows for quick and easy view of the history of each instrument s system suitability (process control) Customizable views for date range, metric type, plot type, etc. 9
10 Assay History and Document Summary in Panorama Gives an overview of the file: Version of Skyline, Proteins, Peptides, precursors, transitions, replicates, calibration curves Under the Calibration curve sub divider, you can see the peptides in the run, navigate to the Figures of merit (FOM) and perform pharmacokinetic (PK) calculations. Gather information about the calibration curve 10
11 Interacting with Quantitative Data in Panorama: Details By selecting the details of the peptide of interest, you are able to drill down to see the individual peptide data 11
12 Interacting with Quantitative Data in Panorama: Calibration Curves Panorama reads the information from the Skyline file Summarizes by peptide Ability to export a PDF or image of the standard curve 12
13 Interacting with Quantitative Data in Panorama : Calibration Curves Set up calibration curves by changing the sample type in Skyline and assigning the analyte concentration Under peptide settings, chose regression fit, normalization method, regression weighting, MS level, units, and newly added in Skyline Daily Figures of merit 13
14 Interacting with Quantitative Data in Panorama : Figures of Merit Quickly view figures of merit by peptide for the entire document Easily obtained from the document summary 14
15 Interacting with Quantitative Data in Panorama : Figures of Merit The Figures of Merit report provides the following calculations: Mean - The mean of all replicates for a given sample. CV(%) - Coefficient of Variation for all replicates for a given sample (the standard deviation divided by the mean). Bias(%) Difference between the average values of the replicates for a given sample and the expected value Export Function n - The Number of samples used for above calculations. LOQ - The limit of quantitation, i.e., lowest concentration at which the %CV is less than 30%. ULOQ - The upper limit of quantitation, i.e., the highest concentration for standards at which the %CV is less than 30% SD - Standard deviation of all replicates for a given sample. 15
16 Interacting with Quantitative Data in Panorama : PK Calculations Requires some up-front input into Skyline Annotate Dose, time point, Dose Group, Subject ID, Dose Units 16
17 Interacting with Quantitative Data in Panorama : PK Calculations LabKey server 18.1 Currently supports Single Dose IV non-iv workflows are still in development Chose both C0 and Terminal time points Both Linear and Log-Linear graphs 17
18 Automated and Integrated Data Analysis Chorus Discovery Proteomics, Cloud Based, Qualitative and Quantitative Data Analysis and Storage Skyline Keystone Software for Targeted Protein Quantitation Assay Development and Data Analysis Panorama Server Software for Aggregating and Publishing Targeted Protein Quantitation Data Licensed by PPDM in 2015 (Merck is a Panorama Partner) Study Request Seamless Integration of Discovery Data Analysis, Targeted Method Development, Statistical Analysis, Method Sharing, and Report Generation Electronic notebook 18
19 Acknowledgements Merck PPDM Bioanalytical Group Dan Spellman Lisa O Callaghan BaoJen Shyong Kevin Bateman James Marr II LabKey Josh Eckels Marty Pradere Ron Dashwood Cory Nathe Skyline Brendan MacLean Nick Shulman Vagisha Sharma Michael MacCoss Panorama Partners Program 19
20 THANK YOU!
Skyline & Panorama: Key Tools for Establishing a Targeted LC/MS Workflow
Skyline & Panorama: Key Tools for Establishing a Targeted LC/MS Workflow Kristin Wildsmith Scientist, Biomarker Development LabKey User Conference October 6, 2016 Biomarkers enable drug development Drug
More informationMultiQuant Software 2.0 with the SignalFinder Algorithm
MultiQuant Software 2.0 with the SignalFinder Algorithm The Next Generation in Quantitative Data Processing Quantitative analysis using LC/MS/MS has benefited from a number of advancements that have led
More informationImportant Information for MCP Authors
Guidelines to Authors for Publication of Manuscripts Describing Development and Application of Targeted Mass Spectrometry Measurements of Peptides and Proteins and Submission Checklist The following Guidelines
More informationPushing the Leading Edge in Protein Quantitation: Integrated, Precise, and Reproducible Protein Quantitation Workflow Solutions
2017 Metabolomics Seminars Pushing the Leading Edge in Protein Quantitation: Integrated, Precise, and Reproducible Protein Quantitation Workflow Solutions The world leader in serving science 2 3 Cancer
More informationTools and workflows to simplify method development for targeted MRM methods
Tools and workflows to simplify method development for targeted MRM methods Nikunj Tanna Senior Scientist, Scientific Operations Waters Corporation, Milford, MA 2016 Waters Corporation 1 Journey of a large
More informationSpectroDive NEXT GENERATION TARGETED PROTEOMICS. Integration of Ready-Made Panels Improved Workflow for Custom Panels
SpectroDive NEXT GENERATION TARGETED PROTEOMICS Integration of Ready-Made Panels Improved Workflow for Custom Panels IMPROVING MULTIPLEXING FOR TARGETED PROTEOMICS Novel targeted proteomics workflows can
More informationPharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study
Pharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study Featuring the SCIEX BioBA Solution Xi Qu 1, Shuyu Hou 1, Meghan McCann 1, Caroline Becker 1, Xun Wang 1,
More informationXevo G2-S QTof and TransOmics: A Multi-Omics System for the Differential LC/MS Analysis of Proteins, Metabolites, and Lipids
Xevo G2-S QTof and TransOmics: A Multi-Omics System for the Differential LC/MS Analysis of Proteins, Metabolites, and Lipids Ian Edwards, Jayne Kirk, and Joanne Williams Waters Corporation, Manchester,
More informationIncreasing Throughput and Efficiency with Exactive LC/MS and Triple Quadrupole LC/MS/MS
Increasing Throughput and Efficiency with Exactive LC/MS and Triple Quadrupole LC/MS/MS Nicholas Duczak Thermo Scientific Annual Mass Spectrometry Users Meeting Somerset, NJ October 12th, 2011 Lead Finding
More informationUNIFI: The user environment Ken Eglinton Nordic User Training, September 2013
UNIFI: The user environment Ken Eglinton Nordic User Training, September 2013 2013 Waters Corporation 1 What is UNIFI? Single Platform for Chromatography, Mass Spectrometry, Data Management and Laboratory
More informationDISCOVERY AND VALIDATION OF TARGETS AND BIOMARKERS BY MASS SPECTROMETRY-BASED PROTEOMICS. September, 2011
DISCOVERY AND VALIDATION OF TARGETS AND BIOMARKERS BY MASS SPECTROMETRY-BASED PROTEOMICS September, 2011 1 CAPRION PROTEOMICS Leading proteomics-based service provider - Biomarker and target discovery
More informationSimultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MS E Data Analysis
Simultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MS E Data Analysis Mark Wrona, 1 Paul Rainville, 1 Eric Langlois, 2 Nigel Ewing,
More informationSpectronaut Pulsar X. Maximize proteome coverage and data completeness by utilizing the power of Hybrid Libraries
Spectronaut Pulsar X Maximize proteome coverage and data completeness by utilizing the power of Hybrid Libraries More versatility in proteomics research Spectronaut has delivered highest performance in
More informationMRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics
Product Bulletin MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP System Technology Overview Targeted peptide quantitation is a rapidly growing
More informationAgilent Software Tools for Mass Spectrometry Based Multi-omics Studies
Agilent Software Tools for Mass Spectrometry Based Multi-omics Studies Technical Overview Introduction The central dogma for biological information flow is expressed as a series of chemical conversions
More informationTargeted Proteomics Environment
Targeted Proteomics Environment Signal processing for quantitative proteomics Brendan MacLean MacCoss Lab Spectrum-based Quantification Uses peptide spectrum matches already calculated Spectral counting
More informationInformatics and High Resolution QTof MS. How can we ask better questions and get better answers in DMPK? Mark D. Wrona
Informatics and High Resolution QTof MS How can we ask better questions and get better answers in DMPK? Mark D. Wrona Principal Scientist Pharmaceutical & Life Sciences, Waters Corporation Pacific Northwest
More informationNext Generation Technology for Reproducible and Precise Proteome Profiling
7 th Czech Mass Spectrometry Conference April 11th, 2018 Next Generation Technology for Reproducible and Precise Proteome Profiling Lars Kristensen, Ph.D. Application and training specialist The world
More informationThermo Scientific Mass Spectrometric Immunoassay (MSIA) Pipette Tips. Next generation immunoaffinity. Robust quantitative platform
Thermo Scientific Mass Spectrometric Immunoassay (MSIA) Pipette Tips Next generation immunoaffinity Robust quantitative platform Immunoaffinity sample preparation Thermo Scientific Mass Spectrometric Immunoassay
More informationStreamlined and Complete LC-MS Workflow for MAM
Streamlined and Complete LC-MS Workflow for MAM Multiple Attribute Methodology (MAM) for Biotherapeutic Attribute Monitoring and Purity Testing BV MAM WORKFLOW Define Track Quantify Purity New Peaks Report
More informationAGILENT S BIOINFORMATICS ANALYSIS SOFTWARE
ACCELERATING PROGRESS IS IN OUR GENES AGILENT S BIOINFORMATICS ANALYSIS SOFTWARE GENESPRING GENE EXPRESSION (GX) MASS PROFILER PROFESSIONAL (MPP) PATHWAY ARCHITECT (PA) See Deeper. Reach Further. BIOINFORMATICS
More informationA Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System
A Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in
More informationAppendix. Table of contents
Appendix Table of contents 1. Appendix figures 2. Legends of Appendix figures 3. References Appendix Figure S1 -Tub STIL (41) DVFFYQADDEHYIPR (55) (64) AVLLDLEPR (72) 1 451aa (1071) YLNENQLSQLSVTR (1084)
More informationA Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System
A Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in human
More informationSample Preparation Automation and QQQ Workflows for Peptide Quantitation in DMPK
Sample Preparation Automation and QQQ Workflows for Peptide Quantitation in DMPK Alex Zhu & Yanan Yang LCMS Applications Scientist Agilent Technologies Why is Agilent Workflow a Good Choice for DMPK? AssayMAP
More informationProteinPilot Report for ProteinPilot Software
ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Powerful mass spectrometers like
More informationSCIEX OS Software One Touch Productivity
SCIEX OS Software One Touch Productivity Accuracy, Simplicity and Efficiency What SCIEX OS Software is all About Mass spectrometry and the data challenge Mass spectrometry continues to provide more comprehensive
More informationIncrease throughput: spend less time cutting and. any CDS into Agilent's ELN
Increase throughput: spend less time cutting and pasting. Smart Import your results directly from any CDS into Agilent's ELN e-seminar, November 18 th, 2009 Agenda Introduction on Agilent Software and
More informationHigh Resolution Accurate Mass Peptide Quantitation on Thermo Scientific Q Exactive Mass Spectrometers. The world leader in serving science
High Resolution Accurate Mass Peptide Quantitation on Thermo Scientific Q Exactive Mass Spectrometers The world leader in serving science Goals Explore the capabilities of High Resolution Accurate Mass
More informationCollaboration to Insight
Collaboration to Insight November 2016 HUMAN HEALTH ENVIRONMENTAL HEALTH 2015 PerkinElmer Safe Harbor Statement This document and/or presentation shows current intentions regarding product features, behavior,
More informationThermo Scientific Insight 2 Software for Evolution 200 Series UV-Vis Spectrophotometers. Comprehensive solutions Simplified workflows
Thermo Scientific Insight 2 Software for Evolution 200 Series UV-Vis Spectrophotometers Comprehensive solutions Simplified workflows Accelerate your analysis No matter how you use your UV-Visible spectrophotometer,
More informationAccelerating Throughput for Targeted Quantitation of Proteins/Peptides in Biological Samples
Technical Note Accelerating Throughput for Targeted Quantitation of Proteins/Peptides in Biological Samples Biomarker Verification Studies using the QTRAP 5500 Systems and the Reagents Triplex Sahana Mollah,
More informationreverse transcription! RT 1! RT 2! RT 3!
Supplementary Figure! Entire workflow repeated 3 times! mirna! stock! -fold dilution series! to yield - copies! per µl in PCR reaction! reverse transcription! RT! pre-pcr! mastermix!!!! 3!!! 3! ddpcr!
More informationProudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment API 3000 LC/MS/MS Unprecedented Performance: An advanced LC/MS/MS system
More informationUNIFI: an innovative enabling platform Ken Eglinton Nordic User Training, September 2013
UNIFI: an innovative enabling platform Ken Eglinton Nordic User Training, September 2013 2013 Waters Corporation 1 Waters Laboratory Informatics Product Portfolio MassLynx Mass Spectrometry Software NuGenesis
More informationfaster, confident more path to all the information in your samples. Agilent MassHunter Workstation Software Our measure is your success.
Agilent MassHunter Workstation Software faster, A confident more path to all the information in your samples. Our measure is your success. products applications soft ware services MassHunter Workstation
More informationPAVING THE WAY FOR ASSESSING IN VIVO DYNAMICS OF MULTIPLE QUALITY ATTRIBUTES FOR PROTEIN THERAPEUTICS
PAVING THE WAY FOR ASSESSING IN VIVO DYNAMICS OF MULTIPLE QUALITY ATTRIBUTES FOR PROTEIN THERAPEUTICS CASSS Mass Spec September 21, 2017 Haihong Zhou Principal Scientist, Biologics, Vaccines & Bioanalytics
More informationLC/MS Based Quantitation of Intact Proteins for Bioanalytical Applications
LC/MS Based Quantitation of Intact Proteins for Bioanalytical Applications Alex Zhu, Ph.D. Agilent Technologies Wilmington, DE ASMS,2017-06 1 Outline Introduction on intact protein quantitation Agilent
More informationJohn Mehl, Bogdan Sleczka, Eugene Ciccimaro, Christian Caporuscio, Ekaterina Deyanova, Richard Huang, Timothy Olah, Celia D Arienzo
Bioanalytical strategies for the quantitation of in-vivo site specific modifications of therapeutic antibodies in early discovery cyno studies using IP-LC-MS John Mehl, Bogdan Sleczka, Eugene Ciccimaro,
More informationApplication Note. Authors. Abstract
Automated, High Precision Tryptic Digestion and SISCAPA-MS Quantification of Human Plasma Proteins Using the Agilent Bravo Automated Liquid Handling Platform Application Note Authors Morteza Razavi, N.
More informationGary A. Schultz, Advion BioServices, Inc. Ithaca, NY 14850
Validation of an immunoprecipitation, digestion and immunoaffinity LC/LC/nanoLC-MS/MS assay for human b-nerve growth factor (NGF) and implementation in support of clinical trials Gary A. Schultz, schultzg@advion.com
More informationSWATH to MRM Builder App
SWATH to MRM Builder App OneOmics Project RUO-MKT-11-3837-A OneOmics Project Workflow Overview Proteomics Facility Samples SWATH-MS Spectral Reference Libraries Protein Expression Workflow Analytics *.wiff
More informationThermo Scientific TSQ Quantiva Triple-Stage Quadrupole Mass Spectrometer. Extreme quantitative performance. with unprecedented ease
Thermo Scientific TSQ Quantiva Triple-Stage Quadrupole Mass Spectrometer Extreme quantitative performance with unprecedented ease Unsurpassed quantitation Continual changes in research and regulatory requirements
More informationOptiFlow Quant Solution for High-Sensitivity Quantitation
OptiFlow Quant Solution for High-Sensitivity Quantitation The Intelligent Union of Sensitivity, Ease-of-Use, and Robustness MicroFlow Sensitivity Analytical Flow Usability Transform Your Quantitation Microflow
More informationApplication Note LCMS-87 Automated Acquisition and Analysis of Data for Monitoring Protein Conjugation by LC-MS Using BioPharma Compass
Application Note LCMS-87 Automated Acquisition and Analysis of Data for Monitoring Protein Conjugation by LC-MS Using BioPharma Compass Abstract Here we describe the development and use of an automated
More informationMetabolomics: Sifting Through Complex Samples
Metabolomics: Sifting Through Complex Samples Thursday, March 14, 2013 Print Jeffrey M. Perkel Look between the covers of any biochemistry textbook and you ll likely find an illustration outlining the
More informationImproving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS
Improving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest and Lei Xiong SCIEX, Redwood City,
More informationA Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery Using HPLC-Chip/ MS-Enabled ESI-TOF MS
Application Note PROTEOMICS METABOLOMICS GENOMICS INFORMATICS GLYILEVALCYSGLUGLNALASERLEUASPARG CYSVALLYSPROLYSPHETYRTHRLEUHISLYS A Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery
More informationSee More, Much More Clearly
TRIPLETOF 6600 SYSTEM WITH SWATH ACQUISITION 2.0 See More, Much More Clearly NEXT-GENERATION PROTEOMICS PLATFORM Every Peak. Every Run. Every Time. The Next-generation Proteomics Platform is here: The
More informationCLSI C60: Assay Validation & Post-Validation Monitoring
CLSI C60: Assay Validation & Post-Validation Monitoring Ross J. Molinaro, MT(ASCP), PhD, DABCC, FACB Medical Director Core Laboratory, Emory University Hospital Midtown Emory Clinical Translational Research
More informationEnabling Systems Biology Driven Proteome Wide Quantitation of Mycobacterium Tuberculosis
Enabling Systems Biology Driven Proteome Wide Quantitation of Mycobacterium Tuberculosis SWATH Acquisition on the TripleTOF 5600+ System Samuel L. Bader, Robert L. Moritz Institute of Systems Biology,
More informationWorld-leading LC/MS/MS technology for quantitation and identification API LC/MS/MS System
World-leading LC/MS/MS technology for quantitation and identification API 3000 LC/MS/MS System rel Accelerate your drug development cycle The API 3000 LC/MS/MS System leads the field in sensitivity, specificity,
More informationProudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment API 150 EX LC/MS System The API 150EX LC/MS System is the most rugged and reliable
More informationLECTURE 8. Biologics and Future Technologies for the Clinical Laboratory
LECTURE 8 Biologics and Future Technologies for the Clinical Laboratory Jack Henion Quintiles Bioanalytical and ADME Labs Lecture 8, Page 1 New and Developing LC/MS Bioanalytical Capabilities: A Representative
More informationAnalysis of Large Scale MRM Studies Using Skyline
Analysis of Large Scale MRM Studies Using Skyline Michael Schirm, PhD Associate Director, R&D Proteomics Skyline User Meeting June 04, 2017 Overview CRO providing mass spec based proteomics services since
More informationZipChip TM. Microfluidic CE-ESI Utility Life Sciences CE-ESI-MS Applications. Please visit us at
ZipChip TM Microfluidic CE-ESI Utility Life Sciences CE-ESI-MS Applications Please visit us at http://www.908devices.com/ // Actionable Intelligence HPMS & On-board Analytics / Slide 1 June 2016 // Presentation
More informationTable 1: CE-MS: Unexploited Opportunities in Process Development?
Table 1: CE-MS: Unexploited Opportunities in Process Development? Session 1 Facilitator: Mei Han, Amgen Inc. Scribe: Yunan Wang, Amgen Inc. Session 2 Facilitator: Göran Hübner, Boehringer Ingelheim Pharma
More informationMETABOLOMICS. Biomarker and Omics Solutions FOR METABOLOMICS
METABOLOMICS Biomarker and Omics Solutions FOR METABOLOMICS The metabolome consists of the entirety of small molecules consumed or created in the metabolic processes that sustain life. Metabolomics is
More informationBioBA High Capacity Enrichment Sample Preparation Kit:
BioBA High Capacity Enrichment Sample Preparation Kit: Biologics Bioanalysis Made Easy Ian Moore 1, Chang Liu 1, Gary Impey 1, Suma Ramagiri 1 1 Sciex, Concord, Canada Common Immunocapture Sample Extraction
More informationThermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer. Extreme quantitative value. with unprecedented ease
Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer Extreme quantitative value with unprecedented ease Unsurpassed value From forensic toxicology to environmental analysis to pharmaceutical
More informationSMCxPRO Immunoassay System Sensitivity You Can Count On
SMCxPRO Immunoassay System Sensitivity You Can Count On The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. Fit for Your Purpose Protein detection at every level. Your
More informationAntibody Discovery at Evotec
Antibody Discovery at Evotec - Overview - Evotec Antibodies Adding value to our partners research Innovative and flexible solutions from target ID to pre-clinical candidate The people A wide therapeutic
More informationImproving Sensitivity for an Immunocapture LC-MS Assay of Infliximab in Rat Plasma Using Trap-and-Elute MicroLC-MS
Improving Sensitivity for an Immunocapture LC-MS Assay of in Rat Plasma Using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest
More informationYour bridge to. better medicines
Your bridge to better medicines At a Glance Anapharm Bioanalytics is a client-oriented, GLP-certified, FDA-inspected, GCP-compliant and ANVISA-certified bioanalytical contract research organization (CRO)
More informationPharmaceutical LC/MS Solutions from Agilent Technologies
Pharmaceutical LC/MS Solutions from Agilent Technologies Application Compendium Overview LC/MS plays a key role in the drug discovery and drug development process. Since the introduction of electrospray
More informationSimplifying ImmunoAffinity Capture Workflow
CAE TUDY MART Digest ImmunoAffinity (IA) Kit implifying ImmunoAffinity Capture Workflow Rapid, ensitive, LC-RM Quantitative Analysis of Proteins in Plasma As pharmaceuticals grow more efficacious, reporting
More informationUsing WinNonlin with UNIFI: Enhanced Export for the Metabolite Identification Application Solution
Using WinNonlin with UNIFI: Enhanced Export for the Metabolite Identification Application Solution Yun Alelyunas, Paul Rainville, and Mark Wrona; Waters Corporation GOAL To demonstrate a simple workflow
More informationExpand Your Research with Metabolomics and Proteomics. Christine Miller Omics Market Manager ASMS 2017
Expand Your Research with Metabolomics and Proteomics Christine Miller Omics Market Manager ASMS 2017 New Additions to Agilent Omics Workflows Acquisition Ion Mobility Q-TOF IM All Ions MS/MS Find Features
More informationAPPLICATION NOTE. Library. ProteinPilot RESULTS. Spectronaut
PPLICTION NOTE Fast Proteome Quantification by Micro Flow SWTH cquisition and Targeted nalysis with Spectronaut Reveal Deep Insights into Liver Cancer iology DD ProteinPilot RESULTS HELTHY CNCER Panhuman
More informationQuantification of Host Cell Protein Impurities Using the Agilent 1290 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System
Application Note Biotherapeutics Quantification of Host Cell Protein Impurities Using the Agilent 9 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System Authors Linfeng Wu and Yanan Yang
More informationFusion Analytical Method Validation
Fusion QbD Software System Fusion Analytical Method Validation The Only Software That Has It All! 100% aligned with FDA/ICH Quality by Design (QbD) guidances! Can be used for LC and Non-LC methods (e.g.
More informationHigh-Throughput Glycan Screening to Remove Biopharmaceutical Development Bottlenecks
High-Throughput Glycan Screening to Remove Biopharmaceutical Development Bottlenecks Mark Lies, Marcia Santos, and Tingting Li SCIEX Separations, Brea, CA Introduction Development of biopharmaceuticals
More informationZipChip TM. Microfluidic CE-ESI Biotherapeutics CE-ESI-MS Applications. Please visit us at
ZipChip TM Microfluidic CE-ESI Biotherapeutics CE-ESI-MS Applications Please visit us at http://www.908devices.com/ // Actionable Intelligence HPMS & On-board Analytics / Slide 1 June 2016 // ZipChip CE-ESI
More informationThermo Scientific Q Exactive HF Orbitrap LC-MS/MS System. Higher-Quality Data, Faster Than Ever. Speed Productivity Confidence
Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS System Higher-Quality Data, Faster Than Ever Speed Productivity Confidence Transforming discovery quantitation The Thermo Scientific Q Exactive HF hybrid
More informationMetabolite Identification Workflows: A Flexible Approach to Data Analysis and Reporting
Metabolite Identification Workflows: A Flexible Approach to Data Analysis and Reporting Stephen McDonald and Mark D. Wrona Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S The flexibility
More informationNanoLC-Ultra system. data sheet. Introducing the NanoLC-Ultra family of high pressure HPLCs for proteomics research
data sheet NanoLC-Ultra system The new NanoLC-Ultra is Eksigent s third generation system, delivering superior gradient precision at pressures up to 10,000 psi. Introducing the NanoLC-Ultra family of high
More informationMRM Analysis of a Parkinson s Disease Protein Signature
MRM Analysis of a Parkinson s Disease Protein Signature Tiziana Alberio, 1,2 Kelly McMahon, 3 Manuela Cuccurullo, 4 Lee A Gethings, 3 Craig Lawless, 5 Maurizio Zibetti, 6 Johannes PC Vissers, 3 Leonardo
More informationA highly sensitive and robust 150 µm column to enable high-throughput proteomics
APPLICATION NOTE 21744 Robust LC Separation Optimized MS Acquisition Comprehensive Data Informatics A highly sensitive and robust 15 µm column to enable high-throughput proteomics Authors Xin Zhang, 1
More informationThermo Scientific EASY-nLC 1000 System. Effortless, split-free nanoflow UHPLC Top performance in LC/MS
Thermo Scientific EASY-nLC 1000 System Effortless, split-free nanoflow UHPLC Top performance in LC/MS Effortless split-free, nanoflow UHPLC The Thermo Scientific EASY-nLC 1000 is a fully integrated, split-free,
More informationthermo scientific Find out more at thermofisher.com/lims TECHNICAL INFORMATION BULLETIN
thermo scientific TECHNICAL INFORMATION BULLETIN Thermo Scientific SampleManager LIMS and Thermo Scientific Chromeleon CDS Link Key Words Integrated LIMS and CDS, Secure & Compliant Interface, Workflows
More informationQuality Control Measures for Routine, High-Throughput Targeted Protein Quantitation Using Tandem Capillary Column Separation
Quality Control Measures for Routine, High-Throughput Targeted Protein Quantitation Using Tandem Capillary Column Separation Sebastien Gallien 1, Elodie Duriez 1, Amol Prakash, Scott Peterman, Andreas
More informationSimultaneous Quantitation of a Monoclonal Antibody and Two Proteins in Human Plasma by High Resolution and Accurate Mass Measurements
Simultaneous Quantitation of a Monoclonal Antibody and Two Proteins in Human Plasma by High Resolution and Accurate Mass Measurements Paul-Gerhard Lassahn 1, Kai Scheffler 2, Myriam Demant 3, Nathanael
More informationThermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer. Extreme quantitative value. with unprecedented ease
Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer Extreme quantitative value with unprecedented ease Unsurpassed value From forensic toxicology to environmental analysis to pharmaceutical
More informationReady, Set, Test! AACC Conference Mass Spectrometry in the Clinical Lab: Best Practice and Current Applications September 17-18, 2013 St.
Ready, Set, Test! Ross Molinaro, PhD, MLS(ASCP) CM, DABCC, FACB Medical Director, Clinical Laboratories Emory University Hospital Midtown Emory Clinical Translational Research Laboratory AACC Conference
More informationWAIT! Ready, Set, Test! Financial Disclosure. Research/Educational grants/consulting/salary support
Ready, Set, Test! Ross Molinaro, PhD, MLS(ASCP) CM, DABCC, FACB Medical Director, Clinical Laboratories Emory University Hospital Midtown Emory Clinical Translational Research Laboratory AACC Conference
More informationSupplementary Figure 1. Limit of quantitation (LOQ) and limit of detection (LOD) were
Supplementary Figure 1. Limit of quantitation (LOQ) and limit of detection (LOD) were determined for SIL peptides in buffer solution. Presented in this figure are the regression analysis to demonstrate
More informationValidation of a concentration assay using Biacore C
GE Healthcare Application Note 48 Biacore systems Validation of a concentration assay using Biacore C Guideline for development of a GxP - compliant concentration assay Support for informed decision-making
More informationFusion Analytical Method Validation
Fusion QbD Software Platform Fusion Analytical Method Validation The Only Software That Has It All! 100% aligned with FDA/ICH Quality by Design (QbD) guidances! Can be used for LC and Non-LC methods (e.g.
More informationAgilent 6000 Series LC/MS Solutions. Confidence in Quantitative and Qualitative Analysis
Agilent 6000 Series LC/MS Solutions Confidence in Quantitative and Qualitative Analysis Agilent 6000 Series LC/MS Solutions SOLUTIONS THAT SET THE STANDARD Your work with Agilent instruments benefits from
More informationImprove the performance of your Proteomics LC-MS facility with Quic
APPLICATION NOTE Improve the performance of your Proteomics LC-MS facility with Quic FEATURES An active queue of per-run QC analyses that checking supports real-time folder monitoring A multitude of readouts
More informationEffectively Dealing with Transition Selection and Data Analysis for Multiplexed Quantitative SRM-MS Assays across Multiple Vendor Instruments
Effectively Dealing with Transition Selection and Data Analysis for Multiplexed Quantitative SRM-MS Assays across Multiple Vendor Instruments Susan Abbatiello, Ph.D. Skyline User s Meeting May 20, 2012
More informationThermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer. Extreme quantitative value. with unprecedented ease
Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer Extreme quantitative value with unprecedented ease Unsurpassed value From forensic toxicology to environmental analysis to pharmaceutical
More informationImproving Productivity with Applied Biosystems GPS Explorer
Product Bulletin TOF MS Improving Productivity with Applied Biosystems GPS Explorer Software Purpose GPS Explorer Software is the application layer software for the Applied Biosystems 4700 Proteomics Discovery
More informationExperimental Design and MS Workflows for Omics Applications. David A. Weil, Ph.D. Senior Application Scientist Schaumburg, IL
Experimental Design and MS Workflows for Omics Applications David A. Weil, Ph.D. Senior Application Scientist Schaumburg, IL David_Weil@agilent.com 1 Acknowledgements: Support Team AFO Metabolomics Team
More informationfor water and beverage analysis
Thermo Scientific EQuan MAX Plus Systems Automated, high-throughput LC-MS solutions for water and beverage analysis Pesticides Pharmaceuticals Personal care products Endocrine disruptors Perfluorinated
More informationAPI 3200 LC/MS/MS system
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment API 3200 LC/MS/MS system Reliability, Reproducibility, and Confidence in Your
More informationIntroduction to Kalabie Electronic Lab Notebook. May 2009
Introduction to Kalabie Electronic Lab Notebook May 2009 Kalabie ELN: part of the integrated Agilent solution Multiple inputs Data coming from instruments and other documents/ information used to document
More informationAgilent AssayMAP Bravo Platform AUTOMATED PROTEIN AND PEPTIDE SAMPLE PREPARATION FOR MASS SPEC ANALYSIS
Agilent AssayMAP Bravo Platform AUTOMATED PROTEIN AND PEPTIDE SAMPLE PREPARATION FOR MASS SPEC ANALYSIS GET FAST, ACCURATE, REPRODUCIBLE RESULTS The Agilent AssayMAP platform is an easy to use yet flexible
More informationVyužití cílené proteomiky pro kontrolu falšování potravin: identifikace peptidových markerů v mase pomocí LC- Q Exactive MS/MS
Využití cílené proteomiky pro kontrolu falšování potravin: identifikace peptidových markerů v mase pomocí LC- Q Exactive MS/MS Michal Godula Ph.D. Thermo Fisher Scientific The world leader in serving science
More informationMassHunter Profinder: Batch Processing Software for High Quality Feature Extraction of Mass Spectrometry Data
MassHunter Profinder: Batch Processing Software for High Quality Feature Extraction of Mass Spectrometry Data Technical Overview Introduction LC/MS metabolomics workflows typically involve the following
More information