Mass Detection for the Chromatographer Waters ACQUITY QDa Detector

Transcription

1 Mass Detection for the Chromatographer Waters ACQUITY QDa Detector Warren B Potts III Senior Director Americas Marketing warren_potts@waters.com 2017 Waters Corporation 1

2 Agenda Overview of technology Applying the technology Summary 2017 Waters Corporation 2

3 Technology has changed a lot since the 90s 2017 Waters Corporation 3

4 Is this what you think of when you think mass spectrometry 2017 Waters Corporation 4

5 MS is finally stackable QDa PDA 2017 Waters Corporation 5

6 The power of mass detection. Identify co-elutions Detect non-chromophoric analytes 2017 Waters Corporation 6

7 The Newest Member of ACQUITY Family: ACQUITY QDa Detector. Provides information rich mass detection option to the family of ACQUITY detectors Provides orthogonal detection for the broad range of applications that benefit from the extra information A robust design for the challenges of constant use with wide variety of chromatographic conditions Seamlessly integrates into the ACQUITY family system in fit, form and function. bridges today s challenges to the tomorrow s solutions 2017 Waters Corporation 7

8 2017 Waters Corporation 8

9 Hard to get it wrong PRE-OPTIMIZED ES± AUTOMATED CALIBRATION ZERO ADJUSTMENT EMPOWER 2&3 Detector concept Adjustment-free & pre-optimized Automated calibration Familiar software 2017 Waters Corporation 9

10 ACQUITY QDa Detector Start up Provides Robust, Reproducible Performance.. Automated resolution and calibration occurs with each start-up to assure that the mass information is accurate and precise ESI interface optimized for UPLC and UPC 2 performance to assure that chromatographic resolution, sensitivity and throughput is preserved.providing familiarity and ease of operation for all analysts 2017 Waters Corporation 10

11 Automated Mass Calibration 2017 Waters Corporation 11

12 All Routine Maintenance is Easily Accessable.. Pre-cut ESI interface provides reproducibly optimized performance Easily replaced Sample Aperture simplifies routine maintenance and restores original performance o Similar in time as replacing PDA lamp 2017 Waters Corporation 12

13 Familiar User Interface Provides Fast Incorporation in Lab.. GUI identical to PDA for setup means very little training is needed to begin using immediately in current lab SOP GUI identical to PDA for data viewing and reporting for accelerating routine use of information rich data to increase confidence in results.accelerates adoption with chemists SOPs within days 2017 Waters Corporation 13

14 Integrated System Control for Common Parameters Using Single Method Editor Provides similar information and format as PDA interface Mass range for expected compounds of interest Ability to choose ESI+, ESI- or both for switching Provides information for the number of points per second based on goals and separation 2017 Waters Corporation 14

15 Advanced Menu Provides Flexibility in Application Specific Analysis Provides detector information that is essential for compound specific analysis Allows the capability of making advanced analysis routine Can be developed for a type of analysis and then chosen as routine in future 2017 Waters Corporation 15

16 ACQUITY QDa Detector s Mass Analysis Window (Empower 3 FR3) Provides Fast Peak ID 2017 Waters Corporation 16

17 Optimizes System Specific Performance and Compatibility and Qualification ACQUITY UPLC ACQUITY UPLC H-Class ACQUITY UPLC I-Class ACQUITY UPC 2 ACQUITY Arc LC and SFC Purification Alliance HPLC Empower 2 FR5 to Empower 3 FR4 MassLynx 4.1.accelerates adoption in all phases of business support 2017 Waters Corporation 17

18 Transform your workflows with mass detection... METHOD DEVELOPMENT SAMPLE PROFILING SYNTHETIC CHEMISTRY PURIFICATION 2017 Waters Corporation 18

19 The benefits of ACQUITY QDa mass detection in method development. PURPOSE Develop Method for Related Substances of Metoclopramide Drug Substance using the ACQUITY UPLC H-Class with PDA and ACQUITY QDa Detectors Sample METHOD DEVELOPMENT Metoclopramide API & related compounds in 50/50 Methanol/Water Method Criteria Total # of Peaks # of peaks with Resolution 2.0 Antiemetic used in the treatment of nausea and vomiting # of peaks with Peak Tailing 1.5 Minimum k* Waters Corporation 19

20 Use mass data to quickly and confidently track peaks Low & High ph with CSH C 18 & Acetonitrile 0.60 Mass data linked to UV chromatogram AU 0.30 Low ph High ph AU Minutes 2017 Waters Corporation 20

21 How do I know that I am picking the best conditions.? Empower Software calculates system suitability parameters Resolution, tailing, k etc Custom calculations can be used to further interrogate data Calculations often done in Excel can be done in Empower Custom reporting Allows users to quickly see data to determine what separations are best 2017 Waters Corporation 21

22 Highly similar UV, differentiating mass Metoclopramide m/z = Metoclopramide Impurity G nm m/z = Waters Corporation 22

23 Confidently assess peak purity without running individual standards. My peak looked pure, but the core MS lab found otherwise Apex Check peak purity using both mass and PDA data in Empower Leading Trailing 2017 Waters Corporation 23

24 Expand capability... Profile and quantify more analytes... SAMPLE PROFILING AND QUANTITATION Cetrimonium bromide (CTAB) is a common pharmaceutical raw material Lack of UV chromophore but readily detected by ACQUITY QDa USP methodology involves a time consuming multi-step titrimetic analysis Improved sensitivity and selectivity 2017 Waters Corporation 24

25 Expand capability... Profile and quantify more analytes... Easy to use, robust mass detection allows quick and reproducible CTAB analysis Readily deployable in QC environment 2017 Waters Corporation 25

26 Summary ACQUITY QDa Detector is a mass detector designed for integration with a liquid chromatography system Synergistic system elements to enhance utility, flexibility and robustness in routine and advanced applications Simplify lab analyses Reduce number iterative confirmation in method development runs Can reduce number of specialty detectors (ELSD, FL, RI) Orthogonal detection beneficial over a range of applications Confirm peak identity and homogeneity Analysis of UV transparent analytes Selective detection for complex matrices Confirmation of yield in chemical syntheses Simplification of purification process Peak tracking and quality assessment in separation development Reduce number of specialty detectors 2017 Waters Corporation 26

27 2017 Waters Corporation 27