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1 Tubes Simport, 0.2ml Amplitube Tubes With Attached Caps Designed for oil-free operation, these 0.2ml tubes are made of transparent, superior quality grade polypropylene. Their ultra-thin walls ensure rapid thermal transfer and a significant reduction in cycle and reaction time. The attached, hinged caps are either dome or pierceable flat-topped and can be used with thermal cycler heated lids. The tubes have a frosted writing area for sample identification and are packaged in tamperproof, safety-lock bags. Certified RNase, DNase and DNA-free. Amplitube Tubes With Integral Shield The caps on these tubes have an integral shield that prevents contamination with the surface of the lid to Cat. No. Cap Colour Pack Price Cat. No. Cap Colour Pack Price Dome Natural 1000 POA Flat Natural 1000 POA Dome Blue 1000 POA Flat Blue 1000 POA Dome Green 1000 POA Flat Green 1000 POA Dome Red 1000 POA Flat Red 1000 POA Dome Yellow 1000 POA Flat Yellow 1000 POA Amplitube Tubes Without Integral Shield The ideal tube design when centrifugation is necessary to Cat. No. Cap Colour Pack Price Cat. No. Cap Colour Pack Price Dome Natural 1000 POA Flat Natural 1000 POA Dome Blue 1000 POA Flat Blue 1000 POA Dome Green 1000 POA Flat Green 1000 POA Dome Red 1000 POA Flat Red 1000 POA Dome Yellow 1000 POA Flat Yellow 1000 POA Simport, 0.5ml Amplitube Tubes With Attached Caps Designed for oil-free operation, these 0.5ml tubes are made of polypropylene with a polished surface, a conical shape and a round bottom for maximum sample recovery. They have an etched writing surface and are graduated in 0.1ml increments. The attached hinged caps are either dome or pierceable flattopped and can be used with thermal cycler heated lids. Packaged in tamperproof, resealable bags to Cat. No. Cap Colour Pack Price Cat. No. Cap Colour Pack Price Dome Natural 1000 POA Flat Natural 1000 POA Dome Blue 1000 POA Flat Blue 1000 POA Dome Green 1000 POA Flat Green 1000 POA Dome Red 1000 POA Flat Red 1000 POA Dome Yellow 1000 POA Flat Yellow 1000 POA Simport, 0.2ml Amplitube Tubes With Separate Caps This thin wall 0.2ml tube is very useful when processing smaller volumes. It offers optimum contact with thermal cycler blocks and the ultra-thin wall ensures rapid thermal transfer. Manufactured from polypropylene with a highly polished surface and a round bottom for maximum sample recovery. Sealing can be achieved using 8-cap strips, in either a dome or pierceable flat top design, which can be cut to length (see page 718). Packaged in tamperproof, resealable bags. Certified RNase, DNase and DNA-free to Natural 1000 POA Red 1000 POA Blue 1000 POA Yellow 1000 POA Green 1000 POA 717

2 Tubes to Simport, 0.2ml Amplitube Strips With Separate Caps Amplitube reaction strips include 8 or 12 integral polypropylene 0.2ml tubes, which are more easily handled than single tubes. They precisely fit standard well spacing and can be used with 8- and 12- channel hand-held pipettors. They can also be cut to any length and used as individual tubes if required to Non-attached dome and flat-top cap strips are available. Packaged in tamperproof, resealable bags. Certified RNase, DNase and DNA-free. Amplitube Reaction Tube Strips of Natural 125 POA Red 125 POA Blue 125 POA Yellow 125 POA Green 125 POA Amplitube Reaction Tube Strips of Natural 125 POA Red 125 POA Blue 125 POA Yellow 125 POA Green 125 POA Simport, Cap Strips for Amplitube Tubes Polypropylene cap strips available in a dome or pierceable flat-top design. They provide a perfect closure during the whole thermal cycle, are autoclavable and can be cut to any length. Certified RNase, DNase and DNA-free. Dome Cap Strip Cat. No. Strip Colour Pack Price Cat. No. Strip Colour Pack Price Natural 125 POA Red 125 POA Blue 125 POA Yellow 125 POA Green 125 POA Natural 125 POA Flat Cap Strip Cat. No. Strip Colour Pack Price Cat. No. Strip Colour Pack Price Natural 125 POA Red 125 POA Blue 125 POA Yellow 125 POA Green 125 POA Simport, 0.2ml Amplitube Strips With Individual Caps Convenient 0.2ml 8-tube strips with individually attached caps. The caps have integral seals preventing contamination with the surface of the lids. The polypropylene strips precisely fit standard well spacing and can be used with 8-channel hand-held pipettors. They can also be cut to any length and used as individual tubes. Packaged in tamperproof, resealable bags. Certified RNase, DNase and DNA-free. Cat. No. Cap Colour Pack Price Cat. No. Cap Colour Pack Price Flat Natural 125 POA Dome Natural 125 POA Flat Blue 125 POA Dome Blue 125 POA Flat Green 125 POA Dome Green 125 POA Flat Red 125 POA Dome Red 125 POA Flat Yellow 125 POA Dome Yellow 125 POA Simport, 0.2ml Amplitube Strips With Attached Strip Caps These polypropylene 0.2ml 8-tube strips have attached dome cap strip. The strips will precisely fit standard well spacing and can be used with 8-channel hand-held pipettors. Packaged in tamperproof, resealable bags. Certified RNase, DNase and DNA-free Natural 125 POA Red 125 POA Blue 125 POA Yellow 125 POA Green 125 POA 718

3 Plates Greiner, ThermoQuick Microplates The ThermoQuick 96-well microplates for molecular biology applications are designed to fit the well profiles of all major thermal cyclers. These plates offer increased capacity and convenience when processing multiple samples. Manufactured from polycarbonate to withstand 135 C. Lids, sealing film and foil are available (see page 722). DNAse and RNAse free. Cat. No. Description Suitable for Pack Price Type 1 microplates Biometra/Ericomp 50 POA Type 2 microplates Hybaid 50 POA Type 3 microplates MJ Research 50 POA Type 4 microplates Perkin Elmer 50 POA Type 5 microplates Techne POA Type 6 microplates Techne POA Lid for plate types 1 4 as above 50 POA Lid for plate type 5 only as above 50 POA Simport, Amplate -96, -48, -24 Thin Wall Plates These 96-well plates are thin-walled for rapid thermal transfer and suitable for use with all 96-well cyclers. Each well has a capacity of 0.2ml. They are precision-moulded from polypropylene to ensure well-to-well and plate-to-plate uniformity, with smooth, inert surfaces on which enzymes and nucleic acids do not bind. Domed and flat cap strips, SecureSeal thermal sealing film and foil and the Amplate mat can all be used for sealing. Their flexible design allows them to be easily cut into sections of 24, 32 or 48 tubes and precut plates are available for 48 tubes (6x8) and 24 tubes (3x8). On the 96-well plate an alphanumeric grid helps sample identification and the bottom right corner is cut away to aid orientation. The Amplate is easy to seal since no cylindrical walls extend above the plate. They are autoclavable and packaged in tamperproof, resealable bags of 10 plates. Certified RNase, DNase and DNA-free to to Amplate -96 plate (8x12 wells) Natural 100 POA Red 100 POA Blue 100 POA Yellow 100 POA Green 100 POA Amplate -48 plate (6x8 wells) Natural 50 POA Red 50 POA Blue 50 POA Yellow 50 POA Green 50 POA Amplate -24 plate (3x8 wells) Natural 50 POA Red 50 POA Blue 50 POA Yellow 50 POA Green 50 POA Simport, Low Profile Amplate -96 Thin Wall Plates These low profile 96-well plates are similar to the regular Simport Amplate series (above) but each of the 96 tubes has a smaller volume, only 100µl, thereby reducing the dead space between sample and cover Natural 100 POA Red 100 POA Blue 100 POA Yellow 100 POA Green 100 POA 719

4 Plates Simport, Skirted Amplate -96 Thin Wall Plates These 100µl 96-well plates have a skirt round the plate, providing a bar coding and labelling area. They can be handled by robotic handling equipment, are ideal for automated pipetting systems and are suitable for use with all 96-well cyclers. They are thin-walled and designed for rapid thermal transfer and are precision-moulded from polypropylene to ensure well-to-well and plate-to-plate uniformity. Quite superior to polycarbonate plates, they are impermeable to water vapour. Domed and flat cap strips, SecureSeal thermal sealing film and foil and the Amplate mat can all be used for sealing. An alphanumeric grid helps sample identification. To facilitate orientation the top left corner of the plate is cut away. The Amplate is easy to seal since no cylindrical walls extend above the plate. These plates are available in a choice of natural and four colours and also opaque black or white for chemiluminescent and fluorescent procedures. They are autoclavable and packaged in tamperproof, resealable bags of 10 plates. Certified RNase, DNase and DNA-free. Standard Skirted Amplate -96 plates Natural 100 POA Red 100 POA Blue 100 POA Yellow 100 POA Green 100 POA Opaque Skirted Amplate -96 plates Opaque black 100 POA Opaque white 100 POA Simport, Amplate Raised Rim Thin Wall Plates Offering just the right rigidity for automation, these three 96-well plates, made in a standard 8x12 configuration, are perfectly suited for high performance thermal cycling. These plates offer an alternative to existing Robbins, Corning and Perkin-Elmer models. The Robbins and Corning style plates have a 3mm raised rim around each tube well and the Perkin-Elmer style plate has a 1mm rim. The Corning and Perkin-Elmer style plates are skirted, providing a surface on which a barcode can be affixed. Plates on all models can be cut into strips of 16 wells (2x8). Domed and flat cap strips, SecureSeal thermal sealing film and foil and the Amplate mat can be used for sealing. The Perkin-Elmer style plates accommodate micromats only. An alphanumeric grid helps sample identification and the top left corner of the plate is cut away to facilitate orientation. These plates are autoclavable and packaged in tamperproof, resealable bags of 10 plates. Certified RNase, DNase and DNA-free. Cat. No. Alternative To Well Capacity, µl Colour Pack Price Robbins plate 250 Natural 100 POA Robbins plate 250 Blue 100 POA Robbins plate 250 Green 100 POA Robbins plate 250 Red 100 POA Robbins plate 250 Yellow 100 POA Corning plate 250 Natural 100 POA Corning plate 250 Blue 100 POA Corning plate 250 Green 100 POA Corning plate 250 Red 100 POA Corning plate 250 Yellow 100 POA Perkin-Elmer plate 200 Natural 100 POA Perkin-Elmer plate 200 Blue 100 POA Perkin-Elmer plate 200 Green 100 POA Perkin-Elmer plate 200 Red 100 POA Perkin-Elmer plate 200 Yellow 100 POA 720

5 Plates Simport, Skirted Amplate -384 Thin Wall Plates This plate has been developed for high volume laboratory work. It is precision-moulded from polypropylene to ensure well-to-well and plate-to-plate uniformity. The Amplate -384 wells have a volume of 40µl and can be used with reaction volumes from 2 to 30µl. All wells on the plate are thin-walled to ensure efficient and fast heat transfer. Although it has 384 wells it can still be filled using automated fluid handling systems or standard multichannel pipettors. In order to offer more surface contact between the plate and the sealing medium, such as thermal foil and adhesive sealing films, there are no cylindrical walls extending above the plate. The plates are skirted to allow barcoding and labelling on the sides and also to make them compatible with automated pipetting systems. Holes on the sides allow for precise and accurate plate positioning and removal. An alphanumeric grid helps sample identification and one corner of the plate is cut away to facilitate orientation. These plates are available in a choice of natural and four colours and also opaque black or white for chemiluminescent and fluorescent procedures. They are autoclavable and packaged in tamperproof, resealable bags of 10 plates. Certified RNase, DNase and DNA-free Standard Skirted Amplate -384 plates Standard skirted Amplate-384 plate Natural 100 POA Standard skirted Amplate-384 plate Blue 100 POA Standard skirted Amplate-384 plate Green 100 POA Standard skirted Amplate-384 plate Red 100 POA Standard skirted Amplate-384 plate Yellow 100 POA Opaque Skirted Amplate -384 plates Opaque skirted Amplate-384 plate Opaque black 100 POA Opaque skirted Amplate-384 plate Opaque white 100 POA 96-Tube Disposable Matrix Plate These polypropylene matrix plates comprise of 96x0.2ml thin wall tubes joined to form a microplate complex. They have a rigid top surface to the plate making the matrix easier to handle and may be cut to 2x48 or 4x24 sections if required. Plates have extended wings for easier labelling and can be sealed with caps or sealing mats (see below). Both deep well and shallow well versions are available, sterile and non-sterile. The shallow well plate eliminates much of the dead air space, reducing evaporation during the heating cycles tube matrix plate, deep well 10 POA tube matrix plate, deep well (sterile) 10 POA tube matrix plate, shallow well 10 POA tube matrix plate, shallow well (sterile) 10 POA Caps and Sealing Mats for Matrix Plates The 96 tube matrix plate can be sealed using domed 8-cap strips, pierceable 8-cap strips or the double sided, re-usable sealing mats, which can be autoclaved at least 50 times Domed 8 cap strip 120 Strips POA Pierceable 8 cap strip 120 Strips POA Reusable Sealing Mats 5 POA

6 Plate Sealing Simport, SecureSeal Thermal Adhesive Tape for This transparent sealing tape consists of a 2.0mil polyolefin film coated on one side with a pressure sensitive acrylate adhesive which does not interfere with cycle reactions. It is ideal for reducing well-towell contamination and/or spill over in sensitive applications where the minimisation of evaporation and vapour loss is critical. SecureSeal thermal film was developed with the assistance of a major cycler manufacturer for applications. Not only does it offer low-autofluorescence but it will prevent vapour loss and is thermostable and functional from -70 to +100 C. Manufactured RNase, DNase and DNA-free. DMSO resistant. Note: Performance may depend on the specific collection/sample vessel used as well as the specific conditions subjected to Cat. No. Description Sterile Pack Price SecureSeal thermal film No 1000 POA SecureSeal thermal film Yes 1000 POA Simport, SecureSeal Aluminium Sealing Foil SecureSeal aluminium sealing foil is ideal for manual sealing of microplates and plates and also for high throughput applications. Its adhesive backing makes it easy to apply and no heat sealer is required. It will resist temperatures from -86 to +130 C. The foil can be pierced with a pipette tip for easy access to samples. It is recommended that the Amplate roller is used to ensure a perfect seal to eliminate the risk of evaporation, see below. DMSO resistant SecureSeal aluminium foil 100 POA Simport, Amplate Roller This roller is designed for use with SecureSeal sealing film and aluminium foil for ensuring a secure seal on microplates, deep well plates and plates. The roller is made of medium hard rubber and the heavy duty handle has a comfort grip. 100mm long Amplate roller 1 POA Simport, Amplate Sealing Mat This flexible sealing cover is used on 96-well plates along with clip down and screw top thermal cyclers and has been proven to be a secure and effective way of sealing. It is re-usable and therefore cost effective. Dimples on one side of the mat ensure correct location over the tubes. The mat is autoclavable Amplate mat Blue 5 POA Greiner, ThermoQuick Microplate Seals This sealing film or metal foil is designed for use with the ThermoQuick plates Sealing tape, film 100 POA Sealing tape, metal foil 100 POA

7 Racks 8-Channel Pipette Reservoirs Combine and connect reservoirs and tube carriers using different colours to designate liquid, reagent, sample or rinse material Low volume reservoir for use with any 8-channel micropipette 0.2ml tube carrier accepts individual tubes or 8 tube strips All are manufactured in polypropylene 10ml maximum volume Reservoirs are graduated to 8ml Cat. No. Description Dimensions (lxwxh), mm Pack Price Polypropylene Reservoir Natural reservoir 90x20x20 50 POA Blue reservoir 90x20x20 50 POA Yellow reservoir 90x20x20 50 POA Polypropylene Reservoir 8000 and tube carrier Natural 90x20x42 50 POA Blue 90x20x42 50 POA Yellow 90x20x42 50 POA (Polymerase Chain Reaction) patents are owned by Hoffman-La Roche, Inc. Racks with Lid and Pipetting Reservoir Option A range of 96 place (8x12) racks, which accept individual 0.2ml tubes in 8 or 12 strips. The racks have alphanumerics for sample identification, and are supplied with an innovative sliding lid for added security and stacking. Each rack has a label/tag holder and connect feature for attaching multi-channel pipette reservoirs, see above. The conical cavity profiles 0.2ml tubes. The rack is available in a choice of clear or blue polystyrene as well as polypropylene racks with conical cavity and lid - clear polystyrene 5 POA racks with conical cavity and lid - blue tint polystyrene 5 POA racks with conical cavity and lid - natural polypropylene 5 POA (Polymerase Chain Reaction) patents are owned by Hoffman La Roche Inc. Simport, Plate Combi-Box and Combi-Rack The Combi-Box can be used as a storage rack and a workstation. The white base will accept all 96- and 384-well plates and an easy to remove transparent cover allows easy viewing of the contents. Being only 40mm high, the stackable Combi-Box saves space on the lab bench and on refrigerator and freezer shelves. The Combi-Rack is an innovative support that can hold up to 96 tubes or 12 strips of 8 tubes with caps, with each hole identified using the alphanumeric grid. This stackable rack stands on 4 legs and can be placed on a lab counter or in a refrigerator or freezer. Made from polypropylene it can withstand temperatures from -80 to +121 C. It is also ideal for carrying and storing, freezing and transporting reagents and specimens. For storage, simply place the Combi-Rack in the Combi-Box and replace the cover Combi-Box White 5 POA Combi-Rack Blue 5 POA shown with

8 Racks Workstation Racking System This novel racking system comprises two racks with lids, either of which may be used individually or combined as needed. Both racks are alpha-numerically labelled for identification. Workstation Rack with Lid This is a multi-tube Workstation for effectively handling a variety of tube sizes i.e. 32x0.2ml thin wall single tubes (not strips), 16x0.5ml thick/thin wall tubes, up to 24x1.5ml Microcentrifuge tubes, up to 24x2.0ml Microcentrifuge tubes or a combination of 1.5/2.0ml tubes. This rack accepts the Satellite 96 place 0.2ml rack which clips onto the Workstation (over the existing 0.2ml holes) when 0.2ml tubes/strips/plates are needed in an 8x12 format. 0.2ml Satellite Rack with Lid This 96 place (8x12) rack accepts 0.2ml tubes, 8 or 12 strips and matrix plates for a variety of pre/post sample processing applications and storage down to -80 C. Cat. No. Description Colour Dimensions Pack Price (wxdxh), mm Complete Workstation System Neon Blue 215x115x50 5 POA Complete Workstation System Neon Green 215x115x50 5 POA Complete Workstation System Neon Orange 215x115x50 5 POA Complete Workstation System Neon Pink 215x115x50 5 POA Complete Workstation System Neon Yellow 215x115x50 5 POA Workstation Racks Neon Blue 215x115x50 5 POA Workstation Racks Neon Green 215x115x50 5 POA Workstation Racks Neon Orange 215x115x50 5 POA Workstation Racks Neon Pink 215x115x50 5 POA Workstation Racks Neon Yellow 215x115x50 5 POA Satellite Racks Neon Blue 127x87x33 5 POA Satellite Racks Neon Green 127x87x33 5 POA Satellite Racks Neon Orange 127x87x33 5 POA Satellite Racks Neon Pink 127x87x33 5 POA Satellite Racks Neon Yellow 127x87x33 5 POA Mini-Workstation/storage Container for Transparent polypropylene, stackable storage box and workstation for 0.2ml tubes and strips. When removed from the storage box, the insert becomes a free-standing workstation for Storage Box with insert 10 POA 96-Well Rack with Hinged Lid Sturdy polypropylene rack for working with 0.2ml tubes. Accommodates the standard 8 or 12 tube strips. This rack is ideal for pre- or post- sampling applications. The tube wells are easily identified with raised and imprinted numbers and letters. The rack features a hinged lid to provide flexibility. Available in assorted colours (blue, green, pink, yellow and orange) or natural. Cat. No. Description Colour Tube size, ml Pack Price well rack Assorted POA well rack Natural POA Transfer Tray This handy transfer tray is a thin sheet of high impact polypropylene designed to rest perfectly over a 96- well tube rack. It allows the simultaneous lifting of multiple, individual 0.2ml tubes from a rack. Available in packs of five clear trays or assorted bright colours transfer trays Assorted 5 POA transfer trays Natural 5 POA is a registered trademark owned by Hoffman-LaRoche Inc. 724