(College / School / Department): College of Natural Sciences, School of Biological Sciences

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(College / School / Department): College of Natural Sciences, School of Biological Sciences RISK ASSESSMENT TITLE: Use of Bio Medical Science Laboratory Location / Building / Area: Brambell Building, C4 Activity (Summary): General use of Bio Medical Sciences Laboratory by staff and. This assessment does not cover the use of hazardous substances or equipment not listed below. Additional assessments may be as indicated on this Risk Assessment Form. Date of Assessment: 14 th October 2009 Name of Assessor: John Latchford / Suzanne Barnes / Louise Thurlow What are the dangers/hazards? Slips / Trips and Falls Electrical Hazards Personal Contamination Who might be harmed and how? What are you already doing to prevent harm? What further action is necessary? Action by whom Floor in good condition Bags and coats stored in lockers Power points desk mounted to remove risk of trailing leads Adequate storage is provided Academic Supervisor regularly undertakes inspections All equipment PAT Tested User checks carried out see Information Sheet LS2 - Electrical Safety COSHH Assessments are undertaken Good laboratory practices are implemented Howie lab coats worn. Lab coats folded inside out for storage Suitable footwear worn Hair tied back Appropriate PPE worn eg gloves as directed No eating, drinking or application of cosmetics allowed in Lab Laboratory benches sanitized after use Hands washed in designated sink regularly and prior to leaving Laboratory All spillages to be reported immediately and cleared in accordance with Information Sheet CS5 - Emergency Spill Procedure Regular monitoring is undertaken All defects to be reported to Technical Staff immediately All suspected contamination to be reported immediately to Supervisor. Do not leave the laboratory until authorised to do so Lab coats regularly laundered by specialist contractor

Sharps eg needles, scalpels and broken glass Care taken when using scalpels, especially when changing blades All sharps disposed of in sharps container All breakages reported immediately Sharps containers to be closed when full and disposed of promptly via specialist Waste Contractor Broken glass to be disposed of in designated bins All sharps injuries must be reported Manual Handling Team lifting for heavy items Equipment stored appropriately eg heavy items and glassware stored below shoulder height Identify need for manual handling training and risk assessment and provide if Appropriate access equipment to be provided Emergency procedures eg fire and first aid Spills of hazardous substances Fire Staff, and the Emergency Services All personnel receive Induction before working in Laboratory Action to Take signs displayed in laboratory Appropriate fire fighting equipment eg extinguishers provided Regular fire drills undertaken PEEPs undertaken where All personnel receive Induction before working in Laboratory including emergency spill procedure Spills cleared promptly Appropriate equipment provided eg spill kits Materials used to clean spills placed in specific bins and disposed of by Specialist Waste Contractor Flammable chemicals and activities kept to a minimum Local fire fighting equipment provided Combustible materials kept to a minimum Good level of fire safety awareness amongst staff Fire alarm system installed Fire drills regularly undertaken Fire and smoke detection within the building Liaison with Emergency Services regarding building fire risk takes place Check first aid provision Ensure staff and aware of 333 number All spillages to be reported immediately and cleared in accordance with Information Sheet CS5 - Emergency Spill Procedure Undertake specific Risk Assessment if a process may introduce a heightened fire risk

Exposure to hazardous substances chemical and biological Ensure specific COSHH Assessments are undertaken for the use of all hazardous agents Ensure safe storage of all chemicals see Information Sheet CS3 - Chemical Storage Academic Supervisors Laboratory EQUIPMENT SAFETY refer to Electrical Hazards above Autoclaves burns, manual handling, chemical and biological hazards Microwave Oven burns, chemical and biological hazards Use restricted to authorized trained personnel Autoclave operated in accordance with Information Sheet LS4 Safe Use of Autoclaves Item is regularly inspected for electrical safety Microwave operated in accordance with Information Sheet LS3 Safe Use of Microwaves Autoclave to be serviced annually Vessel to be inspected according to Pressure Systems Safety Regulations 2000 Ensure details of autoclave notified to Insurers Chief Technician Insurance Fumehoods potential exposure to hazardous vapours, gases and particulates and fire Fumehood operated in accordance with Information Sheet LS6 Safe Use of Fume Hoods Fume hoods to be tested annually Estates

Micro and Midi Centrifuges mechanical injuries, chemical and biological hazards Micro Centrifuge operated in accordance with Information Sheet LS5 Safe Use of Micro Centrifuges PCR Machine exposure to hazardous substances and burns Care taken not to touch hot parts of machine Warning notices displayed Microscopes frequent prolonged use can lead to eye strain, headaches and upper limb disorders. Risk of exposure to hazardous substances Heating Blocks and Hot Plates burns, contact with sharps if containers break, fire if incorrect container used or substances heated inappropriately, exposure to hazardous substances Users trained in the correct set up and use of microscopes Height adjustable chairs supplied Regular breaks taken Warning notices displayed Care taken to avoid hot parts of machinery Heat resistant gloves worn as appropriate Appropriate heat and chemical resistant containers used Materials never left unattended on hot plate If liquid spilt on microscope isolate power immediately and notify Consider use of monitor if microscope is to be used regularly for extensive periods of time If a spillage occurs, isolate power immediately and notify Ensure equipment is electrically isolated after use Balances and Ph Meters exposure to hazardous substances Balances cleaned after use If liquid is spilt on a balance, isolate power immediately and notify

UVP Illuminator burns to skin and eyes from UV light, exposure to ethidium bromide Reference made to ethidium bromide COSHH Assessment UV light source interlocked with operation of the door NItrile gloves worn when handling gels containing ethidium bromide Interlock to be checked by regularly All defects to be reported to Laboratory Electrophoresis equipment and power supplies electric shock and exposure to hazardous chemicals, particularly ethidium bromide when handling agarose gel electrophoresis equipment Nitrile gloves worn when handling agarose gel electrophoresis equipment Gel tanks examined for cracks, leaks, exposed wires Gel tank lids firmly closed before switching on the electrical supply If leaks occur during electrophoresis isolate equipment immediately and inform Laboratory Spectrophotometers electrical hazards, exposure to hazardous chemicals, manual handling Care taken when lifting and moving. Team lifting used if Investigate the use of lighter equipment to reduce manual handling issues