Curriculum for the Bachelor Degree in Biotechnology

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1 The curriculum for the B.S.c. degree in is (15) credit hours are distributed as follows:- No Requirements University Requirements Compulsory Elective Faculty Requirements Specialization Requirements Compulsory Elective Supportive Specialization Requirements Total Credit Hours

2 First : University Requirement (24 Cr.H.) A: Compulsory Requirements (21 Cr.H.): Course No Course Title Arabic (1) Arabic (2) English (1) English (2) Computer Skills (1) Military Sciences National Education Cr.H. Weekly Hours Lecture Lab Prerequisite B: Elective University Requirements : (6 Cr.H.) The student is allowed to select (6 Cr.H.) from the university elective courses offered by faculties other than the faculty of Agricultural Technology. Course No. Course Title Cr.H Communication skills Introduction to Psychology Jordanian Society Sport for All Islamic Culture Administration and Economic Concepts Agriculture in Jordan Environment and Society The Orthodox Caliphs History

3 Second: Faculty Requirements: (26 Cr.H.) Course No. Course Title Cr.H Calculus (1) General Biology (1) General Biology (2) General Biology Lab (1) General Biology Lab (2) General Chemistry (1) General Chemistry (2) General Chemistry - Lab 1 (1) General Chemistry Lab 1 (2) Physics for Agricultural students Physics for Agricultural 1 students Lab Computer Skills (2) * Prerequisite Weekly Hours Lecture Lab Prerequisite * * * * * Third: Specialization Requirements: (70Cr.H. ) A- Compulsory Requirements: (64 Cr.H.) Course No. Course Title Cr. H. Weekly Hours Prerequisite Lecture Lab Microbiology Plant Physiology Plant Tissue Culture Plant General Genetics Biostatistics Cell Biology Molecular Biology Animal Physiology Field Training Seminar Graduation Project Food

4 Course No. Course Title Cr. H. Weekly Hours Prerequisite Lecture Lab Animal Microbial Genetics Molecular Genetics Genetic Engineering Bioinformatics Immunology Virology Microbial Every three credit hours equal (140 hours) field training B: Elective Specialization Requirements: (6 Cr. H.) to be selected from the following list: Course No. Course Title Cr. H. Weekly Hours Prerequisite Lecture Lab Principles of Environmental Science Principles of Plant Protection Principles of Agricultural Extension Environmental Biosafety Special Topics Fourth: Supportive Specialization Requirements : (12 Cr. H. ) Course No. Course Title Cr. H. Weekly Hours Lecture Lab Prerequisite Principles of Plant Production Organic Chemistry Analytical Chemistry Biochemistry

5 First semester First Year Second Semester Course No. Course title Cr. H. Course No. Course title Cr. H Arabic Arabic English English Computer Skills (1) Calculus (1) General Biology (1) General Biology (2) General Biology -Lab (1) General Biology -Lab 1 (2) General Chemistry (1) General Chemistry (2) General Chemistry-Lab (1) General Chemistry-Lab 1 (2) Total 17 Total 17 First semester Second Year Second Semester Course No. Course title Cr. H. Course No. Course title Cr. H Computer Skills (2) Principles of Plant Production Physics for Agricultural Microbiology Students Physics for Agricultural National Education Students-Lab Analytical Chemistry - Elective Specialization Requirement Organic Chemistry - Compulsory University Military Sciences Requirement Total 16 Total 15

6 First semester Third Year Second Semester Course No. Course title Cr. H. Course No. Course title Cr. H Biochemistry Plant Biostatics Molecular Biology Cell Biology Animal Physiology Plant Physiology Microbial Genetics Genetics Microbial Food Elective Specialization Requirement Total 18 Total 18 First semester Fourth Year Second Semester Course No. Course title Cr. H. Course No. Course title Cr. H Plant Tissue Culture Animal Virology Immunology Molecular Genetics Genetic Engineering Compulsory University Bioinformatics Requirement Field Training Graduation Project Seminar 1 Total 18 Total 16

7 Al-Balqa Applied University Course Description Arabic Language 1 (-0) This course contains a set of linguistic skills in different levels such as the sounds of the Arabic language, its morphology, syntax, rhetoric, lexicography and writing. It includes as well as on applications on the use of Arabic dictionaries and applications on some writing skills indispensable for scholars in their working lives. It also contains a refined set of Quranic, poetry and short stories of Arabic texts to give the learners the opportunity to be in contact with and to appreciate these texts. Pre-req. Arabic 99 or passing Arabic Level Exam Arabic Language 2 (-0) This course aims to develop student's skills in writing and expression and to provide him with the necessary and basic ones in order to employ them in his daily use, and enable him to stand on the origins of various Arabic scripts, and access to various forms of technical and functional old and new writing. Pre-req English 1 (-0) Is a complete graded course for foreign learners of English; it covers the skills of listening, speaking, reading, and writing; as well as improving pronunciation and building vocabulary; particular emphasis is placed on listening and speaking; the language of the course is British English. Pre-req. English 99 or passing English Level Exam English 2 (-0) Is a continuation of English I Course in terms of approach and methodology However, it covers a new range of interesting topics, and provides a more advanced grammar syllabus in a communicative context as well, therefore, this topic tends to be a continuation of ENGL 101 goals. Pre-req Computer Skills 1 (0-6) An introduction to computing and the broad field of information technology. Topics covered include the basic structure of digital computer systems; operating systems; application software; data communication and networks; and the Internet hands-on learning emphasizes windows (latest operating systems); MS- Office; and the Internet Military Science (-0) This course aims at providing university students with general military culture and illustrating the overall military capabilities of the armed forces to defend the homeland and its role in development and service of the Jordanian society through lectures and field visits National Education (-0) The course of National Education is one of the compulsory requirements of all students in Jordanian universities, national and private. The general intent is to provide students with the theoretical and practical knowledge of the homeland (Jordan), land and people, and to consolidate the love of the homeland and national brotherhood in them, to motivate them to serve the nation and the people consciously and sincerely, to be innovative and competitive by exhortations to achieve the desired progress in various fields of life Calculus (1) (-0) Functions: domain, operations on functions, graphs of functions, trigonometric functions; limits and continuity; the derivative: techniques of differentiation, the chain rule, implicit differentiation; differentials; Roll's theorem; the main value theorem; L'Hôpital's rule; increasing and decreasing functions; concavity; maximum and minimum values of a function, graphs of including rational functions; the indefinite integral; the fundamental theorem of calculus; the area between two curves; inverse functions; logarithmic and exponential functions; hyperbolic functions.

8

9 Al-Balqa Applied University Course Description Computer Skills (2) (2-) Basics of programming, algorithm development using top-down design with syntax and semantics of the C++ programming language, Creating, compiling and executing C++ programs, Primitive data types, operations, control structures, procedures and functions, arrays and classes. Pre- requisite: General Chemistry (1) (-0) Fundamentals of chemical changes, The periodic table and some properties of the elements, Stoichiometry, quantitative chemical relationships, Reactions between ions in aqueous solutions, Oxidation-reduction reactions, Atomic and electronic structure, Chemical Bonding, Chemical bonding and molecular structure General Chemistry Lab (1) 1(0-) Laboratory techniques, Chemical observations, Measurements of volume and mass, Limiting reactant in a salt mixture, Empirical formula for magnesium oxide, Standardization of sodium hydroxide solution, Vinegar analysis, metathesis reactions, Separation and identification of common ions. Determination of Na and K by flame photometer General Chemistry (2) Co- requisite: (-0) States of matter and intermolecular forces, Properties of solutions, Chemical thermodynamics, Chemical kinetics, Chemical equilibrium in gaseous systems, Electrochemistry, Acid-Base equilibrium, Solubility and complex ion equilibrium General Chemistry Lab (2) Pre- requisite: (0-) Molar mass of a volatile liquid and a solid from freezing point depression, Calorimetry, Thermo chemistry and Hess s law, Studies on some factors affecting chemical reaction, Le Chatelier s principle, Equilibrium constant of a chemical reaction using visible spectrophotometer. Oxidation-reduction reactions, Galvanic cells, Electrolytic cells, Faradays laws, Molar solubility and common ion effect. Co- requisite: General Biology (1) (-0) Water (importance for environment and living organism), Macromolecules (structure and function). Structure and function of prokaryote and eukaryotes, cell division (mitosis meiosis). Molecular genetics and DNA technology, Genetics of virus and bacteria. respiration, photosynthesis, fungi, plants (structure, growth, reproduction and development) General Biology (2) (-0) Metabolism and Enzyme, cell membrane (structure and function), Mendel and Gene idea, chromosomal bases of inheritance, origin of species, vertebrate and invertebrate, animal (structure, function, nutrition, reproduction, development, and nervous system), ecology of organisms. Pre- requisite: General Biology Lab (1) 1 (0-) Microscope, cell of plants and animals, (structure and division), chemical and physical properties of cell, enzymes, photosynthesis, cell division (mitosis meiosis). plant tissues, plant organs (Stems, roots, leaves, flowers and fruits).

10 Co- requisite:

11 Al-Balqa Applied University Course Description General Biology Lab. (2) 1 (0-) Diversity of organisms, kingdom of Bacteria, Fungi, Plant, protozoa, and Animals (development and tissue), genetics, molecule genetics, plant tissue culture, animal tissues, animal anatomy I & II. Co- requisite: Microbiology (2-) Discovery of microorganisms - General features - Morphological and structural organization - microbial nutrition - Culture media - Growth curve - Measurement of microbial growth. Ultra structure of bacteria, fungi, algae, protozoa and viruses - Classification of microbes (molecular taxonomy) - Current methods of microbial identification - Plant microbe interactions - Human pathogens - Disinfectants and antibiotics - Environmental microbiology - Metabolism in prokaryotes. Pre-requisite: Plant Physiology (2-) Water and mineral absorption, Translocation and transpiration, photosynthesis, mineral nutrition, plant hormones and plant growth regulators, growth development, germination and dormancy, stress physiology, tension and pressure. Pre- requisite: Plant (-0) Plant genome specialties- genetic engineering of plants, methodology- plant transformation with Ti plasmid of Agrobacterium tumifacians- Ti plasmid derived vector systems- physical methods of transferring genes to plants microprojectile bombardment, electroporation- use of reporter genes in transformed plant cellsmanipulation of gene expression in plants- production of marker free transgenic plants- application of plant genetic engineering- marker aided selection in plant breeding RFLP, RAPD and AFLP- ethics and issues regarding genetically modified plants. Pre-requisite: Plant Tissue Culture (2-) Laboratory organization- sterile techniques- media composition- tissue and organ culture- establishment and maintenance of callus and suspension cultures- somatic embryogenesis- Control of organogenesis and embryogenesishaploid production - embryo culture and rescue in agricultural and horticultural corps- protoplast isolation, culture and regeneration of somatic hybrid- somatic embryogenesis and artificial seeds- mutants for salts, disease, cold, drought, herbicide and other stress conditions- plant micropropagation- meristem culture and virus elimination- shoot tip culture- cryopreservation and germplasm conservation- tissue culture applications. Pre-requisite: General Genetics (2-) Madelian genetics, inheritance of chromosomes, multiple alleles, linkage and crossing over, genetic variation, principles of molecular genetics, principles of population genetics. Pre-requisite Biostatistics (-0) General statistical concepts, measurements of central tendency, measurements of variation, statistical graphs, Chi-square test, t-test, regression, complete randomized design, randomized complete block design. Pre-requisite: Cell Biology (-0) Biological macromolecules - prokaryotic and eukaryotic cells - cell cycle and cell division - detailed structure of the cell membranes - cellular organelles- transport across cell membrane receptors - signal transduction - cell differentiation. Pre-requisite:

12 Al-Balqa Applied University Course Description Molecular Biology (2-) Structure, function and analysis of proteins - structure and analysis of nucleic acids - gene cloninggenetic library - operon and gene regulation - the genetic code and protein synthesis - DNA replication - methods used in molecular biology research - DNA cloning methodologies- recombinant DNA clone selection and characterization- polymerase chain reaction, restriction mapping, DNA sequencing and hybridization- applications of recombinant DNA technology in medicine, industry, agriculture, aquaculture and environmental protection. DNA protein interaction- eukaryotic RNA polymerase and promoters- general transcription factors of eukaryotes- transcription activators and repressors of eukaryotes. Pre-requisite: Animal Physiology (2-) Structure and function of the nervous system - cardiovascular and respiratory systems and their responses to drugs - organization and structure of the endocrine system and the functions of hormones - male and female reproductive physiology. Pre-requisite: Seminar 1 (1,0) Self-study the objective of the course is to impart training to the student to collect literature from various sources on a given topic in the field of biotechnology. The student will learn to make written and oral presentations of the information obtained by self-study. The topics for this course will cover all aspects of biotechnology Graduation Project (0-0) A theoretical and/or experimental investigation of a fundamental or practical problem in applied biology and biotechnology Field Training 6 (0-0) Training the students in research centers and laboratories that related to biotechnology in order to give them scientific and practical experience Food (-0) Principles of biochemical engineering- alcoholic beverages production- genetics and food biotechnologymicroorganisms-based processes and products- modern advances in food biotechnology- biosensors for biological monitoring- novel biotechnology and food safety. Pre-requisite: Animal (-0) Genetic Engineering in animals- transformation of animal cells- cloning vectors and expression vectorsanimal viral vectors and yeast vectors- vaccines and hormones- production of useful proteins in transgenic animals- AIDS- oncogenes and anti-oncogenes- phage display technology- signal transduction- baculoviruses in biocontrol and foreign gene expression- biotechnology of aquaculture- transgenic animals -in vitro fertilization and embryo transfer- use of nucleic acid probes and antibodies in clinical diagnosis and tissue typing- mapping of human genome- RFLP and applications- gene therapy- ethical issues in animal biotechnology. Pre-requisite: Microbial Genetics (-0) The evolution of microbial genetics- Early concept of bacterial variation- Adaptation, mutation and selection- Genetics of Bacteria- Genetics of fungi- Genetics of Viruses.

13 Pre-requisite:

14 Al-Balqa Applied University Course Description Molecular Genetics (-0) Identification of DNA as the genetic material- Gene as the unit of mutation and recombination- mutations (molecular nature)- recombination control (models and mechanisms) - gene as the unit of expression- co linearity of gene and polypeptide- elucidation of the genetic code- regulation of gene expression- promoter, operator, terminator and alternator - DNA damage and repair - extrachormosomal heredity- organization of chromosomes- chromosome abnormalities - quantitative inheritance and population genetics - developmental genetics using Drosophila as model system - somatic cell genetics. Pre-Requisite: Genetic Engineering (2-) Gene cloning vectors - Isolation and manipulation of DNA - Characterization of DNA and RNA - Construction of DNA libraries - Preparation of DNA and RNA probes - Screening of DNA libraries for identification of desired clones - Polymerase chain reaction - Expression of cloned genes - Construction of transgenic organisms - Applications of genetic engineering - Recombinant DNA technology - cloning vectors- gene library - cloning of foreign genes - DNA delivery - genetic transformation of prokaryotes - marker genes- gene expression in prokaryotes - recombinant protein production in yeast - mammalian cell expression vectors - directed mutagenesis - transposon mutagenesis - sitespecific recombination Gene targeting. Pre-requisite: Bioinformatics (-0) This course provides an introduction to bioinformatics with a focus on fundamental bioinformatics problems, the tools used to compute solutions to those problems: Bioinformatic databases, pairwise sequence alignment, multiple sequence alignment, gene prediction, gene expression data analysis, phylogenetics, RNA structure prediction, protein structure prediction, comparative genomics, proteomics Pre-requisite: Immunology (-0) Cells and tissues of immune system- types of immunity: innate, acquired, passive and active- antibody structure and function- antibody diversity- major histo compatibility complex- antigen processing and presentation to T cells - antigen recognition and activation of immune response- regulation of immune response- autoimmunity- hyper sensitive reactions- complement system- techniques exploiting antigen antibody reactions- generation of monoclonal and polyclonal antibodies- immunoassays and their relevance to modern living Pre-requisite: , Virology (2-) History - nomenclature system - replication strategies - the major families of viruses infected animals, humans, and plants transmission - isolation and purification - physical properties serology - control measures. Pre-requisite: Microbial (-0) Microbial production of therapeutic agents- vaccines- synthesis of commercial products by recombinant microorganisms- microbial degradation of xenobiotics- production of single cell protein from carbohydratesfermentation - microbial food products- production of industrially important chemicals- biomass utilization of food, fuels and chemicals- role of microbes in dairy products, flavors- protein secretion in bacteriabiological insect control- pollution and waste control- environmental release and monitoring of genetically modified/engineered organisms.

15 Pre-requisite:

16 Al-Balqa Applied University Course Description Principle of Environmental Science (-0) Introduction to environmental science; ecosystem, nutrient cycle, pollution, impact. Environmental pollution; water, soil, air, and noise. Sustainable development Principles of Plant Protection (-0) The importance and history of plant protection with emphasis on the role of plant protection in worldwide food security. To study the basics of Entomology, Plant Pathology, Apiculture and Weed Science.To study the different methods of pest control and the ecological basis of pest management. Pre-requisite: Principles of Agricultural Extension (-0) The concepts about agricultural extension, importance, philosophy, principle, objectives and the role integrated rural development, in addition to rural leadership and its important in agricultural extension, communication process and its role in adoption the agricultural innovation, planning, implementation and evaluation extensional programs by using extension methods and means and giving an idea about actual extensional work in Jordan Environmental (-0) This course discusses the principles and commercial applications of biotechnology to environmental problems. The course is structured to promote self-learning and students' development of Biotech solutions to real-life problems. Individual subject areas include the use of biotechnology to clean up or 'bioremediate' polluted environments as well as the economic recovery of important minerals and precious metals through 'phytomining'. Furthermore, the application of biological systems in preventing pollution by supporting our planet's sustainable development is also explored in subject areas such as 'biofuels' and 'bioplastics' Pre-requisite: Biosafety (-0) Describe current and future issues and concerns associated with biological hazards and its control in diagnostic, treatment, research facilities and decontamination. Describe the statistical and technical problems involved with microbial sampling and current methods for detecting, counting, and identifying microbes in the environment. Discuss the relevant policies, guidelines, and standards that apply to work involving potential exposures to infections microorganisms. Pre-requisite: Special Topics (-0) Any subject that is not included in the curriculum will be submitted under this course if required by the students. Pre-requisite: Principles of Plant Production (-0) Principles of growth and development of cultivated species.methods of propagation. Environmental factors affecting crop production. Culture and use of fruit, vegetable, flower, ornamental, forest and agronomic crops. Pre-requisite: Organic Chemistry (2-) Introduction to organic chemistry. Nomenclature, structure, physical properties, reactions and synthesis of major organic functional groups. Intended for health science majors. Topics include structure and reactions of alkanes, alkenes, alkynes, alkyl halides, and aromatic molecules; nomenclature of organic compounds; stereochemistry; reaction mechanisms and dynamics

17 Prerequisite:020610

18 Al-Balqa Applied University Course Description Analytical Chemistry (2-) survey of the methods of inorganic quantitative analysis, including the methods of gravimetric and volumetric analysis with the use of simple instrumental methods included. Laboratory experiences involving the qualitative and quantitative analysis of chemical compounds including gravimetric, volumetric and spectrophotometric methods. Prerequisite: Biochemistry (2-) An introduction to the chemistry of life processes, the composition of living matter and the changes associated with biological processes. A laboratory course in which the properties of biochemical molecules are explored and common biochemical reactions are examined. Prerequisite:

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