Biosafety Level Host Range Propagation Comments

Transcription

1 Guidelines BSL for Commonly used Viral Vectors Version 1.0 Office of Animal Care and Institutional Biosafety (OACIB) 1737 West Polk Street (MC 672) 206 Administrative Office Building Chicago, IL Phone: Fax: Biosafety levels indicated below are the default levels based on the criteria listed. Depending on gene inserted or manipulations, IBC may determine that project requires increased level based on risk assessment Viral Vector Adeno- Associated Virus (all serotypes) Risk Group Biosafety Level Host Range Propagation Comments In vitro In vivo Humans and Humans and 1 2 2, then 1 Humans and Murine cell line, helper free Human cell line, helper free Any cell line 2) Cannot be used for transfection of human or nonhuman primate cells other BSL2 agent or BSL2 must be used 3) can be 3) are initially housed at only if proven that replication. If not, then animals are housed at ABSL2 for the

2 Adeno (all serotypes) Herpes Simplex Virus without helper helper free 3) are initially housed at 3) are initially housed at only if proven that replication 3) housed at ABSL2 for 3) are initially housed at 3) are initially housed at only if proven that Page 2 of 5 Document #0365

3 Listeria Retro- Lenti Retro- Murine 2 or , then 1 Humans and 1 1 Mice and Rats (ecotropic) 2 1 Mice and Rats (ecotropic) 2 2, then 1 Humans and (amphotropic) 2, 3, or 4 plasmid systems 2 Murine cell line Human cell line Any cell line replication. 3) housed at ABSL2 for 3) are initially housed at 3) housed at ABSL2 for 3) are initially housed at 1) Virus must be replication defective 2) 3) 3) are initially housed at Page 3 of 5 Document #0365

4 Vaccinia 3) housed at ABSL2 for 4) Vaccination required prior to initiation of work, but highly attenuated 3) housed at ABSL2 for 4) Vaccination requirement will be assessed based on documentation of degree of attenuation and safety risk assessment 1 Risk Group 3 for HIV, Risk Group 2 for other lenties packaged such that tropism includes infection of human cells 3 3 or 4 plasmid systems are preferred as these pose the least risk of potential recombination to generate replication competent References 1. Biosafety Considerations for Research with Lentiviral Vectors. Biosafety Considerations for Research with Lentiviral Vectors. Office of Biotechnology Activities Biosafety in Microbiological and Biomedical Laboratories, 5 th Edition, (2009), NIH and CDC. 3. Lenti Fact Sheet. Alliance with OSHA ABSA NIH Guidelines for Recombinant DNA or Synthetic Nucleic Acid Molecules, Office of Biotechnology Activities. Page 4 of 5 Document #0365

5 5. Pathogen Safety Data Sheets and Risk Assessment. Public Health Agency of Canada Page 5 of 5 Document #0365