9EUROGENTEC 2008 Catalogue > > Electrophoresis

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1 159 9EUROGENTEC 2008 Catalogue > > Electrophoresis Apparatus and accessories 160 Mupid -One electrophoresis system 160 Mupid -exu 162 Agarose 164 AgaTabs 164 Molecular weight markers 164 Regular DNA ladders 164 Genotyping 168 Irregular DNA ladders 169

2 Electrophoresis 9 Apparatus and accessories Mupid -One electrophoresis system Performance and safety The Mupid mini gel system has continuously improved throughout the years, always incorporating new technical innovations in terms of conveniency or safety, to best fit your needs. The electronic components and the materials of Mupid -One have been modified and improved to reach the gold standard in electrophoresis. These improvements lead to higher, safety and longer term usage. The Mupid -One is CE marked. CE marking Intelligent power supply Heat resistant materials Multichannel pipette compatible Safety lid with switch slits Compatible with gel casting sets of previous Mupid versions Gel maker set included Intelligent power supply (with soft touch keys) Timer Function The timer can be user-set from 1 to 99 minutes (includes alarm). The continuous and pause modes are available. Voltage Variation Range 7 kinds of output voltages are available. Peak voltage is constant (140 V) and output level changes through pulse control. Very Simple Operation Operations are very simple, pushing Timer, Voltage Selector, Run/Pause/Stop buttons. Current regulation Rather than performing a forced shutdown when peak current is exceeded the Mupid -One controls the voltage so that it can maintain the current at or below peak value for continuous operation. Safe The lid is made of a robust material and has been designed so that it can be removed with only one hand. The water guide will prevent water from directly dripping while opening the cover and will allow good drainage when washing. The cover is directly connected to the power supply; the main power cannot be operated without the lid. Convenient to use With this very convenient electrophoresis system you will be able to make variable sizes of gel. The combs can be changed very easily and the tank can be washed easily thanks to the removable power supply. Up to 104 samples (96 samples + 8 markers) can be easily loaded with a multichannel pipette in 130 x 122 mm gel. Compatible with gel casting set of previous Mupid. Heat resistant gel casting set. > Gel solution up to 100 can be pourd into the Gel Tray. This makes gel casting time shorter. > Clean up the used Gel Tray easily by boiling water. Buffer Drains Buffer drains are at two corners so that buffer can drain away easily. Storage of Set Values Set voltage and timer values used in the last experiment are automatically retained in memory (supports pausing). Washable The electrophoresis cell can be separated from the power supply and is washable. Global Input Voltage Compatibility Variable input voltage of 100 to 240 V supports used virtually anywhere in the world. Individual country power supply cable may differ Eurogentec s.a.s.u.

3 161 Compact Tank size: 183 x 56 x 162 mm Lid size: 197 x 38 x 170 mm Power supply size: 170 x 62 x 75 mm Only 270 to 320 ml of buffer and 50 to 120 ml of agarose will be required to run a gel. Accessories All the accessories are included with the electrophoresis system: gel maker set (3 trays - 4 combs stand). Heat resistant gel casting set > Gel solution up to 100 can be pourd into the Gel Tray. This makes gel casting time shorter. > Clean up the used Gel Tray easily by boiling water. Multichannel-pipette compatible Comb theeth pitch matches the multichannel-pipette and multi*- sample applicable. * The large gel tray can make up to 4 rowes of wells (running length of 2.7 cm), so can handle up to 104 samples (26 x 4 = 104). Description Mupid -One electrophoresis system complete apparatus Mupid -One Reference MU-0041 Separate accessories for Mupid -One Small tray 4 MU-0041-ST Large tray 2 MU-0041-LT Gel Maker Set 2 ST, 1 LT, 4 Combs Gel Maker Stand MU-0041-MS Standard comb 2 MU-0041-SC Gel casting stand 1 MU-0041-SD Tank & lid 1 MU-0041-TK Power supply 1 MU-0041-PS Separate accessories for Mupid -One are also available One-Gel Casting Stand-HR (1) Many combs setting grooves are provided in the stand. Supports a maximum of 4 combs per gel with easy position change. 2. One-Gel Tray-L-HR (1) It can make up to 4 rows of wells (running length of 2.7 cm), so can handle up to 104 samples (26 x 4 = 104). 3. One-Gel Tray-S-HR (2) and Center Partition (1) It can make up a gel for running 26 or 13 samples. Install at the center of Gel Casting Stand for using the Gel Tray-S. 4. One-Comb-HR (4) 4 pieces of comb can be used for L Tray. 1 piece of comb can be used for S Tray (0) (0) Eurogentec s.a.s.u.

4 Electrophoresis 9 Mupid -ex The only original complete mini gel system! Intelligent power supply UV transmittant tank Multichannel pipette compatible Safety lid with switch slits Compatible with gel casting sets of previous Mupid versions Gel maker set included The Mupid mini gel system has continuously improved throughout the years, always incorporating new technical innovations in terms of conveniency or safety, to best fit your needs. The Mupid family has broken with existing trends and has become the standard for many researchers. The Mupid -ex is an ultra-compact electrophoresis system, which is convenient to use, safe and has new technical advantages as detailed hereafter. Intelligent power supply (with soft touch keys) Small-integrated power supply, which is removable before washing the tank and incorpore a main switch. 7 different voltages: V (constant) Timer: 0 to 99 min (with incorporated alarm) Storage of set values: the voltages and timer set values used in the last experiment are automatically kept into memory. Safety cover: for prevention of an electric shock, without the lid, the main power cannot be operated. Convenient to use The see-through lid gives the possibility to observe the gel while it is running. The detection of the bands can be done directly through the tank as it is made of a special material with a high rate of ultra violet ray transmittance. With this very convenient electrophoresis system you will be able to make variable sizes of gel. The combs can be changed very easily and the tank can be washed easily thanks to the removable power supply. Up to 104 samples (96 samples + 8 markers) can be easily loaded with a multichannel pipette in 130 x 122 mm gel. Compatible with gel casting set of previous Mupid. Power supply Safe The lid is made of a robust material and has been designed so that it can be removed with only one hand. The water guide will prevent water from directly dripping while opening the cover and will allow good drainage when washing. The cover is directly connected to the power supply; the main power cannot be operated without the lid. No need to touch the ethidium bromide containing gel. Compact Tank size: 183 x 56 x 164 mm Lid size: 197 x 38 x 169 mm Power supply size: 170 x 62 x 75 mm Only 270 to 320 ml of buffer and 50 to 120 ml of agarose will be required to run a gel Eurogentec s.a.s.u.

5 163 Accessories All the accessories are included with the electrophoresis system: gel maker set (3 trays - 4 combs stand). Separate accessories for Mupid -ex are also available Mupid -exu Description Mupid -exu electrophoresis system Power supply for Mupid -exu Tank & lid for Mupid -exu Reference MU MU-0040-PS MU-0040-TK 1 large tray (130 x 13 x 122 mm) 4 multichannel combs (13 wells of 9 mm or 26 wells of 4.5 mm lanes) Mupid -exu accessories Small tray 4 MU-0040-ST Large tray 2 MU-0040-LT 2 small trays (130 x 13 x 59 mm) Gel maker stand (149 x 20 x 125 mm) Gel Maker Set 2 ST, 1 LT, 4 Combs Gel Maker Stand MU-0040-MS Standard comb (multichannel comb) 2 MU-0040-SC Separate accessories for i-mupid are available and compatible with Mupid -ex /Mupid -One SmartComb: the SmartComb consists of two combs which fit into the regular Gel Maker Set (for i-mupid ). These combs allow you to make slots of 4 different sizes. Gel maker stand 1 MU-0040-SD i-mupid accessories compatible with Mupid -exu & Mupid -One Gel Maker Set 4 ST, 2 LT, 2 Standard Combs 1 stand MU-000S Standard comb L 2 MU-0001-SC Gel Maker Set for i-mupid : 1 Gel Maker Stand, 2 SmartCombs, 4 small trays (55 x 60 mm) and 2 large trays (109 x 61 mm) (0) (0) Eurogentec s.a.s.u.

6 Electrophoresis 9 Agarose Molecular Biology Grade agarose is the ideal multipurpose agarose for any DNA and RNA application. Small Fragment Agarose provide optimal separation of nucleic acid smaller than 1 kb. Description Agarose Molecular Biology Grade Small Fragments AgaTabs Nucleic acid length range 1000 bp 1000 bp 1000 bp Gelling temperature* 37 ~ 39 C 29 ~ 33 C 37 ~ 39 C Melting temperature 88 ~ 90 C 85 C 88 ~ 90 C Gel strength* 1500 g/cm g/cm g/cm 2 DNase or RNase activity ND ND ND DNA binding ND ND ND Electroendosmosis 0.1 ~ ~ ~ 0.2 Sulfate 0.1 % 0.10 % 0.1 % * measured in 1.5 % agarose solution Molecular Biology Grade Description Weight Reference Molecular Biology Grade Agarose 100 g EP g EP kg EP Molecular weight markers Regular DNA ladders SmartLadder The SmartLadder is a ready-to-use molecular weight marker, specially designed for easy quantification as well as size determination. Sizing 14 regularly spaced bands from 200 to bp. The 1000 and bp bands have a higher intensity than the others to allow quick and easy identification. The size of each band is an exact multiple of 100 bp. Small Fragments Description Weight Reference Small Fragments Agarose 50 g EP g EP Quantification Using a standard loading of 5 µl, each band corresponds to an exact quantity of DNA, from 20 to 100 ng. AgaTabs Agatabs is a Molecular Biology Grade agarose provided as a tablet format 300 tablets (0.5 g / tablet) No more weighing Accurate portion amount You avoid tiresome weighing and moreover contamination during weighing. Storage At 4 C for 6 months. Long term storage at 20 C. Avoid multiple freeze-thaw cycles. SmartLadder Description Size Quantity Lanes Reference SmartLadder 200 to bp 750 µg 1000 MW AgaTabs Description Amount Reference AgaTabs Agarose 300 tablets EP Eurogentec s.a.s.u.

7 165 SmartLadder SF Sizing 9 regularly spaced bands from 100 to 1000 bp. The 1000 bp band has a higher intensity than the others to allow quick and easy identification. The size of each band is an exact multiple of 100 bp. Quantification Using a standard loading of 5 µl, the 200, 400, 600, 800 and 1000 bp bands correspond to an exact quantity of DNA from 20 to 100 ng. Storage At 4 C for 6 months. Long term storage at 20 C. Avoid multiple freeze-thaw cycles. SuperLadders SuperLadders come in seven different types ranging from 20 bp all the way to 15 kb. The SuperLadder markers are all derived from cloned and purified DNA fragments, with no overloaded plasmid DNA band to partially obscure or distort DNA bands in adjacent lanes. Every SuperLadder band is composed of multimers of a single subunit, and each band has a perfect 50 % GC contents. Supplied with 6 x gel loading buffer and useful instructions for obtaining high resolution and precise sizing of DNA fragments. SuperLadder-low 1 kb SuperLadder-low 100 bp (lane 1) 10 sharp bands every 100 bp SuperLadder-low 20 bp (lane 2) 50 sharp bands every 20 bp SmartLadder SF Description Size Quantity Lanes Reference SmartLadder SF 100 to 1000 bp 190 µg 400 MW CloneCheck Markers for testing the insertion efficiency in all plasmid vectors image. The CloneCheck contains bands of equal intensity and clearly separated ranging from 1000 to 4361 bp. SuperLadder-low SL-low 20 bp 40 µg MW µg MW SL-low 100 bp 40 µg MW µg MW Fragment size 1000, 1500, 2000, 2500, 3000, 4361 bp Storage at 4 C Supplied with 6 x concentrated loading buffer CloneCheck CloneCheck 50 assays MW-0210-CC (0) (0) Eurogentec s.a.s.u.

8 Electrophoresis 9 SuperLadder-mid1 4 kb DNA Quantitation Standard Kit SuperLadder-mid1 500 bp (lane 1) 8 sharp bands every 500 bp SuperLadder-mid1 100 bp (lane 2) 40 sharp bands every 100 bp Accurately quantified fragments from 100 to 5000 bp. Kits contain 40 µg each of highly purified x bp DNA fragments. DNA Quantitation Standard Kit 5000 bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW bp fragment 40 µg MW SuperLadder-mid2 15 kb SuperLadder-mid1 SL-mid1 100 bp 60 µg MW µg MW SL-mid1 500 bp 75 µg MW µg MW SuperLadder-mid2 1 kb (lane 1) High-intensity bands every 1000 bp SuperLadder-mid2 200 bp (lane 2) High-intensity bands every 200 bp to ± 8 kb, and then every 1000 bp to 15 kb SuperLadder-High 2.5 kb High-intensity bands every 2500 bp 200 bp fragment 40 µg MW bp fragment 40 µg MW Biomarkers Expanding The Frontiers of Double Stranded DNA Sizing Accuracy BioMarkers provide accurate sizing of dsdna bands over different ranges with nine bands of 100 or 500 bp steps with equal intensities. BioMarkers are optimal for determining size standards for gel electrophoresis, capillary electrophoresis, gel filtration chromatography and HPLC. Biomarkers are optimal for reading DNA fragments ranging from 50 to 10 Kb. They are stable at room temperature and are supplied dephosphorylated in order to allow labelling with polynucleotide kinase onto free 5 -termini. Biotin and Rhodamine labelled biomarkers are available. Biomarkers are supplied with 6 x gel loading buffer. These markers are evaluated for resolution, intensity and background on 7.5 % acrylamide and 3:1 NuSieve: Agarose gels with ethidium bromide staining. Store at 4 C. SuperLadder-mid2 SL-mid2 200 bp 60 µg MW µg MW SL-mid2 1 kb 100 µg MW µg MW SL-High 2.5 kb 100 µg MW Biomarkers Low Biomarker Low contains linear doublestranded DNA bands of 50, 100, 200, 300, 400, 500, 525, 700 and 1000 bp. The concentration of each band is 50 ng/ 5 µl of Biomarker Low applied. Biotin and Rhodamine labelled biomarkers are available Eurogentec s.a.s.u.

9 167 Biomarkers Low Description Lanes Quantity Reference Biomarker Low 50 assays 22.5 µg MW-0200-L Biomarker Low biotinylated Biomarker Low Rhodamine 50 assays 22.5 µg MW-0200-LB 50 assays 22.5 µg MW-0200-LR Biomarkers EXT PLUS Description Lanes Quantity Reference Biomarker EXT Plus 50 assays 32.5 µg MW-0200-EPLUS Biomarker EXT Plus biotinylated Biomarker EXT Plus Rhodamine 50 assays 32.5 µg MW-0200-EPLUSB 50 assays 32.5 µg MW-0200-EPLUSR Biomarkers EXT Biomarkers EXT PLUS Biomarker EXT contains linear doublestranded DNA bands of 50, 100, 200, 300, 400, 500, 525, 700, 1000, 1500 and 2000 bp. The concentration of each band is 50 ng/5 µl of Biomarker EXT applied. Biotin and Rhodamine labelled biomarkers are also available. Biomarkers EXT Description Lanes Quantity Reference Biomarker EXT 50 assays 27.5 µg MW-0200-E Biomarker EXT biotinylated Biomarker EXT Rhodamine 50 assays 27.5 µg MW-0200-EB 50 assays 27.5 µg MW-0200-ER BioMarker EXT PLUS contains linear double stranded DNA bands of 50, 100, 200, 300, 400, 500, 525, 700, 1000, 1500, 2000 and 2500 base pairs. The concentration of each band is 50 ng/5 µl of BioMarker EXT PLUS applied except for 1000 bp which is at 100 ng/5 µl applied. Biomarkers High Biomarker High contains linear double-stranded DNA bands of 500, 1000, 1500, 2000, 2500, 3000 bp. The concentration of each band is 50 ng/5 µl of Biomarker High applied. Biotin and Rhodamine labelled biomarkers are available. BioMarker 10 Kb Biomarkers High Description Lanes Quantity Reference Biomarker High 50 assays 15 µg MW-0200-H Biomarker High biotinylated Biomarker High Rhodamine 50 assays 15 µg MW-0200-HB 50 assays 15 µg MW-0200-HR BioMarker 10 kb contains linear doublestranded DNA bands of 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 7.0, and 10.0 kilo bases. The concentration of each band is 50 ng / 5 µl of BioMarker 10 kb applied. Biomarker 10 kb Description Lanes Quantity Reference Biomarker 10 kb 50 assays µg MW Biomarker 10 kb biotinylated Biomarker 10 kb Rhodamine 50 assays µg MW B 50 assays µg MW R (0) (0) Eurogentec s.a.s.u.

10 Electrophoresis 9 Genotyping The markers are fully compatible with all current methodology, equipment and software. It can be used as well on gels as on capillary-based systems. All MapMarkers are at 8 fmoles/band/µl. MapMarker The Next Generation in Single-Stranded Fluorescent DNA Sizing Standards MapMarker are sets of high quality fluorescently labelled DNA fragments designed to provide consistent intensities and migration patterns for DNA sizing standards and are compatible with all fluorescent-based separation instruments systems. MapMarker DNA fragments are uniformly spaced to provide accurate base calling and precision sizing of samples. MapMarker contains singular DNA strands with a single fluorophore. Only single strands are labeled with a single dye; the products are available with all of the most commonly used fluorescent dyes, including rhodamine, tetramethylrhodamine, x-rhodamine and fluorescein. Custom configurations and dyes are also available. All MapMarker are at 8 fmoles/band/µl. MapMarker 400 Description #Lanes Reference MapMarker 400 labelled with FAM MapMarker 400 labelled with TAMRA MapMarker 400 labelled with ROX MapMarker MW FAM 800 MW TMR 800 MW ROX Specially designed for microsatellite sizing standards Made up of single DNA strands of exact size Regular spaced bands from 50 up to 1000 Labelled with FAM, TAMRA, ROX May be used under either denaturing or non denaturing conditions Stable for a minimum of 18 months if stored at 4 C in dark Intensity MapMarker are evaluated for resolution, intensity and background on denaturing polyacrylamide sequencing gels using laser excitation. MapMarkers are free of extraneous peaks over their entire sizing range and are free of pull up signal in other channels. MapMarkers produce consistent within run and between run sizing within their size range and reproducible runs in gels or capillary-based systems. MapMarkers may be used under either denaturing or nondenaturing conditions. Mapmarkers are stable for a minimum of 18 months when stored in the dark at 4 C. MapMarker 400 Specially designed for microsatellite sizing standards Made up of single DNA strands of exact size Regular spaced bands from 70 up to 400 Labelled with FAM, TAMRA, ROX May be used under either denaturing or non denaturing conditions Stable for a minimum of 18 months if stored at 4 C in dark Size (bp) MapMarker 1000 Description #Lanes Reference MapMarker 1000 labelled with ROX MapMarker 1000 labelled with FAM MapMarker 1000 labelled with TAMRA 800 MW ROX 800 MW FAM 800 MW TMR Intensity Size (bp) Eurogentec s.a.s.u.

11 169 Irregular DNA ladders Marker 1: l x Hind III digest Marker 3: l x Hind III + l DNA x EcoR I digest 23.13/ 9.42/ 6.56/ 4.36/ 2.32/ µg/ml / 21.23/ 9.42/ 7.42/ 6.56/ 5.81/ 5.65/ 4.88/ 4.36/ 3.52/ 2.32/ 2.02/ 0.56/ µg/ml Marker 1 Marker 3 Marker µg MW µg MW Marker 3 80 µg MW Marker 2: l x Hind III/EcoR I double digest Marker 4: FX174 x Hae III digest / 5.15/ 4.98/ 4.27/ 3.52/ 2.02/ 1.91/ 1.58/ 1.38/ 0.95/ 0.83/ 0.56/ µg/ml Fragments (bp) : 1353/ 1078/ 872/ 603/ 310/ 281/ 271/ 234/ 194/ 118/ µg/ml Marker 2 Marker 4 Marker 2 80 µg MW µg MW Marker 4 15 µg MW µg MW (0) (0) Eurogentec s.a.s.u.

12 Electrophoresis 9 Marker 5: FX174 x Hinc II digest Marker 7: T4GT7 + T4GT7 x Bgl I digest (1:3) Fragments (bp) : 1057/ 770/ 612/ 495/ 392/ 345/ 341/ 335/ 297/ 291/ 210/ 162/ µg/ml / 49.31/ 40.88/ 23.08/ 21.00/ 17.80/ 12.67/ µg/ml Marker 5 Marker 7 Marker 5 15 µg MW µg MW Marker 7 10 µg MW µg MW Marker 6: l x Sty I digest Marker 8: T4GT7 + T4GT7 x Bgl II digest (1:3) / 7.74/ 6.22/ 4.26/ 3.47/ 2.69/ 1.88/ 1.49/ 0.93/ 0.42/ µg/ml / 56.50/ 18.01/ 17.40/ 17.39/ 14.21/ 10.60/ 8.10/ 5.50/ 5.30/ 4.20/ 3.30/ 1.19/ µg/ml Marker 6 Marker 8 Marker 6 80 µg MW µg MW Marker 8 10 µg MW µg MW Eurogentec s.a.s.u.

13 171 Marker 9: FX174 x Hinf I digest / 713/ 553/ 500/ 427/ 417/ 413/ 311/ 249/ 200/ 151/ 140/ 118/ 100/ 82/ 66/ 48/42/ 40/ µg/ml Marker 9 Marker 9 15 µg MW µg MW Marker 10: pbr322 x Msp I digest / 527/ 404/ 309/ 242/ 238/ 217/ 201/ 190/ 180/ 160/ 147/ 123/ 110/ 90/ 76/ 67/ 34/ 26/ 15/ µg/ml Marker 10 Marker µg MW µg MW (0) (0) Eurogentec s.a.s.u.

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