Chem 321 Lecture 23 - Liquid Chromatography 11/19/13
|
|
- Vanessa Gallagher
- 6 years ago
- Views:
Transcription
1 Chem 321 Lecture 23 - Liquid Chromatography 11/19/13 Student Learning Objectives High Performance Liquid Chromatography With the advent of relatively inexpensive and reliable pumps, the development of a wide range of detectors and very efficient columns, and the ability to analyze nonvolatile materials, high performance liquid chromatography (HPLC) is now as widely used as gas chromatography. Although the principles of chromatographic separations apply to liquid chromatography, the components of a basic HPLC system differ in significant ways from those in a gas chromatograph. In HPLC both the mobile phase and the stationary phase are liquids. This means that they both interact with the sample components, and in fact compete for these components. In our HPLC experiment, the stationary phase consists of a nonpolar, 18- carbon alkane (C 18 ) covalently bonded to small (5 μm diam.) particles of silica. This is known as a reversed-phase column since the stationary phase is less polar than the mobile phase (just the reverse of the liquid chromatography columns used in organic chemistry which have a polar stationary phase like alumina or silica gel). This is also a bonded stationary phase (one that is covalently bonded to the support particles). This stabilizes the stationary phase to a greater extent than when the liquid is simply mechanically coated onto the support. Bonded GC columns are also available. Silica is chemically attacked by basic solutions so these columns are used with liquids having a ph below 8. The small support particles are generally packed into a metal column (a typical size is 4.6 mm inside diameter x 25 cm in length). We are using cartridge columns in our system. These are very short columns (3.5 cm) sealed at each end with a metal frit. Two cartridges are piggy-backed to serve as the stationary phase. Check for Understanding What is the advantage of using two cartridges in succession? When the separation efficiency of these cartridge columns starts to degrade, they are simply discarded because they cost only a fraction of the cost ($100-$300) for a typical 30-cm analytical column. In order to protect the more expensive columns, a guard column is generally used. This is a very short column placed before the analytical column and consists of the same type of stationary phase as the main column (except the support particles are usually larger). This column serves to trap any sample components that might otherwise enter the main column and adhere. As the guard
2 page 2 column gets contaminated, it is replaced at a very modest cost, or perhaps the packing at the inlet end is refreshed. The small size of the packing particles in a HPLC column requires that the liquid mobile phase be pumped through the column using very high pressures (1000's of psi). This requires a pump capable of delivering a very smooth flow of the mobile phase at these high pressures. This component contributes about half the HPLC hardware costs. Flow rates are typically close to 1 ml/min. The mobile phase (and samples) used for HPLC are filtered through micron-size filters and degassed. Removal of dissolved air minimizes the formation of bubbles that can interfere with a flow-through spectrophotometer cell such as we are using in the detector, or the bubbles can create voids in the column that degrade column efficiency. HPLC-grade reagents are available for use in the mobile phase. In our experiment, the sample components are separated using a 55/45 (v/v) methanol/water mobile phase throughout the elution. This is known as an isocratic elution. A very powerful way to enhance the efficiency of separation is to carry out a gradient elution. In this case, the mobile phase composition changes during the elution. You will use a step-wise gradient elution for the separation of cobalt ion in the ion-exchange chromatography experiment. Since the mobile phase is competing with the stationary phase for the sample components, the composition is changed so that the mobile phase becomes more like the stationary phase during the elution. Check for Understanding For a reversed-phase column, how should a methanol/water mobile phase composition be changed for a gradient elution? Variations in the column temperature during elution (temperature programming) is the corresponding approach used in gas chromatography to improve the efficiency of separation.
3 page 3 Injection of the liquid sample onto the HPLC column usually occurs through a valve similar to the one diagramed in Figure W. H. Freeman and Company Figure 16.1 Diagram of a typical injection valve for HPLC A key feature to this injection port is the sample loop. This is a small section of metal capillary tubing that is filled to capacity during the loading process and then flushed into the column when the sample is injected. Thus, a known and very reproducible volume of sample gets injected. Notice that in the load position (Fig. 16.1a), the plumbing connects the syringe port to the sample loop so that the loop can be flushed and filled with the sample. At this point, the mobile phase flow bypasses the loop. Several (3-4) syringe volumes are generally applied to ensure complete filling of the loop. When the valve is moved to the inject position (Fig. 16.1b) the mobile phase flow is re-directed to flush the contents of the loop into the column. Sample loop sizes generally vary from 10s to 100s of microliters. Note that the very small section of tubing between the syringe port and the waste port in the inject mode (Fig. 16.1b) contains the last sample applied to the injection valve. This can be flushed out by rinsing while in the inject position or by using multiple syringe volumes when in the load position.
4 page 4 Detection of the eluted materials is done continuously with a variety of detectors in HPLC. We are using a variable wavelength spectrophotometer cell in our system. This flow-through cell is diagramed in Figure Although the flow-through cell volume is generally only a few microliters this design results in a pathlength of cm. A deuterium lamp serves as the source of the 275-nm radiation that is used to detect the caffeine in our experiment. This detector is also nondestructive, so the separated components could be collected for use if desired W. H. Freeman and Company Figure 16.2 Diagram of a flow-through cell for HPLC Check for Understanding What criterion will you use to identify the caffeine peak in the chromatogram of your unknown? Since you are able to inject the same volume of sample for each HPLC separation, a simple comparison of component peak areas for a standard and sample
5 page 5 can be done to get the concentration in the unknown. For any component A, where C unk and C std are the concentrations of A in the unknown and standard, respectively (same concentration units). However, rather than compare your unknown with a single standard, several caffeine standards will be analyzed and plot of peak area versus standard concentration will be prepared. The caffeine concentration in the sample will be determined from the equation for the linear least-squares line for the calibration curve and the caffeine peak area for the unknown. In our experiment, each standard will be run once and the unknown sample will be run several times. Calculate the concentration of caffeine in the unknown for each run and average the results. Exercises for Liquid Chromatography
Solutions Guide. MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications
Solutions Guide MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications Page 1 of 12 Table of Contents Sample Injection... 3 Two- Selection... 4
More informationHichrom Limited 1 The Markham Centre, Station Road, Theale, Reading, Berkshire, RG7 4PE, UK Tel: +44 (0) Fax: +44 (0)
Hichrom Limited 1 The Markham Centre, Station Road, Theale, Reading, Berkshire, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: www.hichrom.co.uk Table of Contents Sample Injection...
More informationExperiment 11. DNA Composition by HPLC 1,2
Experiment 11 DNA Composition by HPLC 1,2 Introduction This experiment illustrates quantitative analysis by high performance liquid chromatography and biochemical methods of sample preparation. The sample
More information( ) IONPAC ATC-HC ANION TRAP COLUMN
PRODUCT MANUAL IONPAC ATC-3 2-mm ANION TRAP COLUMN (4 x 35 mm, P/N 059661) IONPAC ATC-3 4-mm ANION TRAP COLUMN (9 x 24 mm, P/N 059660) IONPAC ATC-HC ANION TRAP COLUMN (9 x 75 mm, P/N 059604) QUICKSTART
More informationProtein-Pak Hi Res HIC Column and HIC Protein Standard
Protein-Pak Hi Res HIC Column and HIC Protein Standard CONTENTS I. INTRODUCTION II. a. Mobile Phase b. Flow Direction CONNECTING COLUMN TO LC SYSTEM I. INTRODUCTION This offering contains non-porous, polymethacrylate-based
More informationAgilent MetaCarb Ca PLUS Carbohydrate Column Ca ++ Form User Manual Part No. A5205
Agilent MetaCarb Ca PLUS Carbohydrate Column Ca ++ Form User Manual Part No. A5205 WARNING: The Agilent MetaCarb Ca PLUS calcium column is packed with a polymeric material that requires special care. Introduction
More informationAgilent MetaCarb 87H Organic Acids Column H + Form User Manual Part No. A5214
Agilent MetaCarb 87H Organic Acids Column H + Form User Manual Part No. A5214 Warning: The Agilent MetaCarb 87H organic acids column is packed with a polymeric material that requires special care. Introduction
More informationA Simple Rapid and Sensitive Method Development for Quantification of Quetiapine Fumarate in Bulk and Dosage Forms Using RP-HPLC
Human Journals Research Article February 2018 Vol.:11, Issue:3 All rights are reserved by Priyanka Teepoju et al. A Simple Rapid and Sensitive Method Development for Quantification of Quetiapine Fumarate
More information10. Validated Normal Phase HPLC Method for the Determination. Fulvestrant is primarily used in the treatment of hormone receptor
229 10. Validated Normal Phase HPLC Method for the Determination of Fulvestrant in Pharmaceutical Dosage Forms 10.1 Introduction Fulvestrant is primarily used in the treatment of hormone receptor positive
More informationfor Acclaim Carbamate Column
for Acclaim Carbamate Column Product Manual for Acclaim Carbamate Page 1 of 13 Product Manual for Acclaim Carbamate Analytical Columns 5 µm, 4.6 x 250 mm, P/N 072924 3 µm, 4.6 x 150 mm, P/N 072925 3 µm,
More informationA method to pack Agilent Prep C18 Media on Load & Lock Column
A method to pack Agilent Prep C18 Media on Load & Lock Column Application Note Author Liqin Chen, Rong An Life Science and Chemical Analysis Agilent Technologies Shanghai, China Abstract The successful
More informationOptimizing Purified Sample Recovery on Reverse-Phase HPLC Systems
Optimizing Purified Sample Recovery on Reverse-Phase HPLC Systems Application Note 209 Joan Stevens, PhD, Alan Hamstra, Luke Roenneburg, Tim Hegeman (Gilson, Inc.) Craig Esser, PhD, Regina Black, Derek
More informationorder:
HPLC Guard Column Cartridges (Prepacked) ES Industries guard column cartridges offer excellent protection for your analytical column. Adding a guard column to your HPLC system extends the life of your
More informationPreparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions
Preparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions Gary Scharrer and Joan Stevens, Ph.D. Gilson, Inc Middleton, WI www.gilson.com Abstract Purification of synthetic,
More informationPhysical Stability of a Silica- Based Size Exclusion Column for Antibody Analysis
Physical Stability of a Silica- Based Size Exclusion Column for Antibody Analysis Atis Chakrabarti* and Roy Eksteen + Tosoh Bioscience LLC, King of Prussia, PA 19406 *Corresponding Author. + Current address:
More informationTechnical Overview. Author. Abstract. Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany
New Features of the Agilent Method Scouting Wizard for Automated Method Development of Complex Samples Analysis of Large Data Sets by Method Scouting Reports and Automated Adjustment of Flow Rates and
More informationPrefractionator for Reliable Analysis of the Light Ends of Crude Oil and other Petroleum Fractions
Prefractionator for Reliable Analysis of the Light Ends of Crude Oil and other Petroleum Fractions Application Note Hydrocarbon Processing Author Roger L Firor Agilent Technologies, Inc. 2850 Centerville
More informationHigh Throughput Sub-4 Minute Separation of Antibodies using Size Exclusion Chromatography
High Throughput Sub-4 Minute Separation of Antibodies using Size Exclusion Chromatography TSKgel APPLICATION NOTE Introduction Gel Filtration Chromatography (GFC) is a powerful analytical tool in the separation
More informationDetermination of Inorganic Anions in Acid Rain Using a Dedicated High-Pressure Capillary Ion Chromatography System
Determination of Inorganic Anions in Acid Rain Using a Dedicated High-Pressure Capillary Ion Chromatography System Terri Christison and Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA Technical
More informationApplication Note. Authors. Abstract. Introduction. Pharmaceutical
Scalability of Agilent s Across HPLC and UHPLC Instruments Application Note Pharmaceutical Authors Anne E. Mack, William J. Long Agilent Technologies, Inc. 2850 Centerville Road, Wilmington, DE 19808 USA
More informationIntroduction Ron Majors is a Senior Scientist at Agilent. Bill Champion is a chemist in Agilent s HPLC Columns tech support group.
Video Notes LC Troubleshooting Series Ghost Peaks Introduction Ron Majors is a Senior Scientist at Agilent. Bill Champion is a chemist in Agilent s HPLC Columns tech support group. Ghost peaks can come
More informationImproving Resolution and Column Loading Systematically in Preparative Liquid Chromatography for Isolating a Minor Component from Peppermint Extract
Improving Resolution and Column Loading Systematically in Preparative Liquid Chromatography for Isolating a Minor Component from Peppermint Extract Jo-Ann M. Jablonski and Rui Chen Waters Corporation,
More informationINTERNATIONAL PHARMACOPOEIA MONOGRAPH ON ARTEMETHER AND LUMEFANTRINE CAPSULES REVISED DRAFT FOR DISCUSSION
October 2007 RESTRICTED ` INTERNATIONAL PHARMACOPOEIA MONOGRAPH ON ARTEMETHER AND LUMEFANTRINE CAPSULES REVISED DRAFT FOR DISCUSSION World Health Organization 2007 All rights reserved. This draft is intended
More informationAutomated On-Line SDS Removal from Diminutive Protein Samples Prior to Capillary HPLC Application Note 221
Automated On-Line SDS Removal from Diminutive Protein Samples Prior to Capillary HPLC Application Note 221 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman (Gilson, Inc.) Introduction Removing detergent
More informationAPPENDIX III SAMPLE LAB REPORT. Experiment 1. High Performance Liquid Chromatography. Alfred E. Neuman
APPENDIX III SAMPLE LAB REPORT Experiment 1 High Performance Liquid Chromatography Alfred E. Neuman Chemistry 436 September 15, 1999 INTRODUCTION In this experiment, high performance liquid chromatography
More informationAnalysis of Atrazine in Drinking Water at the ppb Level Using New Agilent Reversed Phase LC Columns. Application. Author. Abstract.
Analysis of Atrazine in Drinking Water at the ppb Level Using ew Agilent Reversed Phase LC Columns Application Environmental Author Rongjie Fu Agilent Technologies, Inc. 412 Ying Lun Road Pu Dong, Shanghai
More informationFast Preparative Column Liquid Chromatography (PCLC)
Fast Preparative Column Liquid Chromatography (PCLC) Application Note 224 Joan Stevens, Ph.D. and Gary Scharrer Introduction Preparative HPLC is recognized as a prime method for obtaining pure compounds
More informationDetermination of Oxalate and Other Anions in Bayer Liquor Using Ion Chromatography
Application Note 206 Determination of Oxalate and Other Anions in Bayer Liquor Using Ion Chromatography Introduction The Bayer Process is used to isolate alumina (Al 2 O ) from bauxite. In this process,
More informationInertsil ODS-3 2 µm. Base Silica Physical Properties and Chemical Modification. Base Silica: High Purity Silica Gel % Surface Area: 450 m 2 /g
0 High-Speed Separation Inertsil ODS-3 2 µm Base Silica Physical Properties and Chemical Modification Base Silica: High Purity Silica Gel 99.999% Surface Area: 450 m 2 /g Pore Size: 100 Å Pore Volume:
More informationAutomated 2-D HPLC Using Trap Columns for the Fractionation, Isolation and Screening of Natural Products
Automated 2-D HPLC Using Trap Columns for the Fractionation, Isolation and Screening of Natural Products Application Note 227 Joan Stevens, Ph.D., Alan Hamstra, Tim Hegeman and Luke Roenneburg (Gilson,
More informationENVIRONMENTAL ANALYSIS. Solutions for Your Analytical Business Markets and Applications Programs. Solution Note
ENVIRONMENTAL ANALYSIS A TURN-KEY GUARANTEED ANALYSER FOR THE ROUTINE MEASUREMENT OF GLYPHOSATE AND AMPA IN DRINKING WATER (EUROPEAN DRINKING WATER DIRECTIVE 98/83/EC) AND OTHER ENVIRONMENTAL WATER SAMPLES
More informationPerformance characteristics of the 1260 Infinity Quaternary LC system
Performance characteristics of the 1260 Infinity Quaternary LC system The new standard in HPLC Technical Overview Introduction The Agilent 1260 Infinity LC system consists of modular units that operate
More informationBÜCHI Labortechnik AG
BÜCHI Labortechnik AG Purification BUCHI Chromatography History 1977: BUCHI Fraction Collector 1978: 1 st preparative chromatography system 2008: automated chromatography system 2014: automated high performance
More informationProduct Guide for LudgerSep TM N2 High Resolution Amide HPLC Columns for Glycan Analysis
Product Guide for LudgerSep TM N2 High Resolution Amide HPLC Columns for Glycan Analysis (Ludger Product Codes: LS-N2-4.6x150 and LS-N2-2.0x150) Ludger Document # LS-N2-Guide-v4.0 Ludger Ltd Culham Science
More informationMethod Translation in Liquid Chromatography
Method Translation in Liquid Chromatography Technical Overview Abstract Ronald E. Majors Agilent Technologies, Inc. 2850 Centerville Rd Wilmington, DE 19808 USA With the recent emphasis on high performance
More informationSAFETY AND QUALITY CONTROL
Note: this protocol is optimized for the Breeze software and for purifying peptides with a high concentration >30 mg/ml To purify peptides with high performance liquid chromatography. Samples can be vortexed
More informationION EXCHANGE KIT FOR MAB SEPARATIONS
ION EXCHANGE KIT FOR MAB SEPARATIONS Sepax Technologies, Inc. 5 Innovation Way Newark, Delaware, USA Tel: (32) 366-111 Fax: (32) 366-1151 Toll free: www.sepax-tech.com Content Introduction... 1 Technical
More informationPreparative Purification of Corticosteroids by HPLC; Scalability and Loadability Using Agilent Prep C18 HPLC Columns Application
Preparative Purification of Corticosteroids by PLC; Scalability and Loadability Using Agilent Prep C18 PLC Columns Application Pharmaceuticals Authors Cliff Woodward and Ronald Majors Agilent Technologies,
More informationCORESTA RECOMMENDED METHOD N 61
CORESTA RECOMMENDED METHOD N 61 DETERMINATION OF 1,2-PROPYLENE GLYCOL, GLYCEROL AND SORBITOL IN TOBACCO AND TOBACCO PRODUCTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) (July 2015) 0. INTRODUCTION
More informationAgilent AdvanceBio SEC Columns for Aggregate Analysis: Instrument Compatibility
Agilent AdvanceBio SEC Columns for Aggregate Analysis: Instrument Compatibility Technical Overview Introduction Agilent AdvanceBio SEC columns are a new family of size exclusion chromatography (SEC) columns
More informationOverview of preparative HPLC. Analytical Technologies Limited
Overview of preparative HPLC Analytical Technologies Limited 1.Review of liquid phase chromatography origin develop prospect Mobile phase Tewett 1903 Stationa ry phase origin Separation result Liquid-stationary
More informationHPLC Columns. HILICpak VN-50 2D, 4D MANUAL. Shodex HPLC Columns Europe, Middle East, Africa, Russia
HPLC Columns MANUAL HILICpak VN-50 2D, 4D Shodex HPLC Columns Europe, Middle East, Africa, Russia For technical support please use contact details shown below: SHOWA DENKO EUROPE GmbH Shodex Business Konrad-Zuse-Platz
More informationCORESTA Recommended Method No. 63
Cooperation Centre for Scientific Research Relative to Tobacco Smoke Analytes Sub-Group CORESTA Recommended Method No. 63 DETERMINATION OF TOBACCO SPECIFIC NITROSAMINES IN CIGARETTE MAINSTREAM SMOKE GC-TEA
More informationTechniques for Improving the Efficiency of Large Volume Loading in Preparative Liquid Chromatography
Techniques for Improving the Efficiency of Large Volume Loading in Preparative Liquid Chromatography Jo-Ann M. Jablonski, Andrew J. Aubin, and Wendy Harrop Waters Corporation, Milford, MA, USA APPLICATION
More informationApplication of Agilent AdvanceBio Desalting-RP Cartridges for LC/MS Analysis of mabs A One- and Two-dimensional LC/MS Study
Application of Agilent AdvanceBio Desalting-RP Cartridges for LC/MS Analysis of mabs A One- and Two-dimensional LC/MS Study Application note Biotherapeutics and Biologics Authors Suresh Babu C.V., Anne
More informationINTERNATIONAL RESEARCH JOURNAL OF PHARMACY
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF DOXAZOSIN FOR THE PRESENCE OF DEGRADATION PRODUCTS AND RELATED
More informationRobustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety
Technical Overview Robustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety Authors Mark Sartain, Theresa Sosienski, and Dan Hui Dorothy Yang Agilent Technologies, Inc.
More informationHigh-Throughput LC/MS Purification of Pharmaceutical Impurities
High-Throughput LC/MS Purification of Pharmaceutical Impurities Application Note Small Molecule Pharmaceuticals Author Florian Rieck Agilent Technologies, Inc. Waldbronn, Germany Abstract Legal regulations
More informationHPLC Columns HIC PH-814 MANUAL. Shodex HPLC Columns Europe, Middle East, Africa, Russia. For technical support please use contact details shown below:
HPLC Columns MANUAL HIC PH-814 Shodex HPLC Columns Europe, Middle East, Africa, Russia For technical support please use contact details shown below: SHOWA DENKO EUROPE GmbH Shodex Business Konrad-Zuse-Platz
More informationAgilent SD-1 Purification System. Purify your way SD-1
Agilent SD-1 Purification System Purify your way SD-1 AGILENT SD-1 PURIFICATION SYSTEM PURIFY YOUR WAY WITH HIGH QUALITY SEPARATIONS AT ANY SCALE The Agilent SD-1 Purification System achieves better gradient
More informationFundamentals and Techniques of Preparative HPLC. Parto Zist Behboud Tel: 42108
Fundamentals and Techniques of Preparative HPLC Parto Zist Behboud www.partoz.com Tel: 42108 Today s Overview 1 Basic know-how for up-scaling 2 Practical examples of up-scaling 3 Recycle preparation 4
More informationDionex IonPac CS16-4µm and Dionex IonPac CS16-Fast-4µm
User Manual Dionex IonPac CS16-4µm and Dionex IonPac CS16-Fast-4µm 065623 Revision 01 September 2015 Product Manual for Dionex IonPac CS16-4µm Analytical Column 088584 IP CS16-4µm 4 250 mm 088582 IP CS16-4µm
More informationAutomated alternating column regeneration on the Agilent 1290 Infinity LC
Automated alternating column regeneration on the Agilent 1290 Infinity LC Increasing throughput using two columns alternatively via an ultra-high pressure 2-position/10-port valve Application Note Environmental
More informationImproved Gas Chromatograph Method for the Analysis of Trace Hydrocarbon Impurities in 1, 3-Butadiene
Improved Gas Chromatograph Method for the Analysis of Trace Hydrocarbon Impurities in 1, 3-Butadiene Application Note Hydrocarbon processing industry Author Chunxiao Wang Agilent Technologies (Shanghai)
More informationPreparative, Pilot & Process Columns
Preparative, Pilot & Process Columns 321 Preparative, Pilot & Process Columns Contents Prep Columns... 322-323 Dynamic Axial Compression Columns (DAC)... 324-325 Pilot Columns... 326-327 Chromatography
More informationColumn Watch. Are You Getting the Most Out of Your HPLC Column?
1124 LCGC NORTH AMERICA VOLUME 21 NUMBER 12 DECEMBER 2003 www.chromatographyonline.com Column Watch This month s Column Watch discusses lowdispersion columns, describes how to modify high performance liquid
More informationAutomated Scouting of Stationary and Mobile Phases Using the Agilent 1290 Infinity II Method Development Solution
Automated Scouting of Stationary and Mobile Phases Using the Agilent 129 Infinity II Method Development Solution Technical Overview Authors Edgar Naegele and Sonja Schneider Agilent Technologies, Inc.
More informationRAM DIRECT INJECTION (RESTRICTED ACCESS MEDIA)
RAM CHROMATOGRAPHY RAM DIRECT INJECTION (RESTRICTED ACCESS MEDIA) A Tool for the Separation of Small Molecules in the Presence of Large Biomolecules Conventional Sample Precipitate proteins Centrifuge
More informationthe power of discovery USER GUIDE FOR ORGANIC ACIDS ANALYSIS COLUMNS
the power of discovery USER GUIDE FOR ORGANIC ACIDS ANALYSIS COLUMNS USER GUIDE FOR ORGANIC ACIDS ANALYSIS COLUMNS This manual describes the use of following Organic Acids Analysis Columns Product Name
More informationHPLC 940-LC ANALY TIC AL TO PREPARATIVE LIQUID CHROMATOGRAPHY SOLUTIONS.
HPLC varian, inc. 940-LC ANALY TIC AL TO PREPARATIVE LIQUID CHROMATOGRAPHY SOLUTIONS www.varianinc.com VARIAN, INC. Scale Up to Superior Performance and Reliability The Ultimate in Analytical to Preparative
More informationBioTrap 500 C18/C8 INSTRUCTION MANUAL. for direct injection of up to 500 µl plasma in HPLC. Illustration of a BioTrap 500 particle
BioTrap 500 C18/C8 for direct injection of up to 500 µl plasma in HPLC INSTRUCTION MANUAL Illustration of a BioTrap 500 particle May. 2000 Instruction manual BioTrap 500 C18/C8 1 CONTENTS Page What is
More informationSimultaneous Preparative HPLC Fraction Collection and Column Regeneration
Simultaneous Preparative HPLC Fraction Collection and Column Regeneration Application Note 234 Gary Scharrer and Joan Stevens, Ph.D. (Gilson, Inc.) Introduction Improving separation efficiency and accelerating
More informationREVIEW ON FLASH CHROMATOGRAPHY
International Journal of Pharmacy Review & Research www.ijprr.com REVIEW ON FLASH CHROMATOGRAPHY Dane Ganesh D *, Raka KC, Honde BS, Bhawal GS, Tajane PJ Department of Pharmaceutical Chemistry, S.V.N.H.T
More informationGenotoxicity is the property of a compound
Impurities Analysis in Pharmaceuticals: Genotoxicity is the property of a compound known to have irreversible effects on the structure and functionality of the DNA in cells and cause DNA loss, DNA replication
More informationEtG / EtS Analysis UCT INNOVATION THROUGH CHEMISTRY FORENSICS
FORENSICS UCT EtG / EtS nalysis INNOVTION THROUGH CHEISTRY EtG and EtS nalysis UCT has developed a rapid solution to combat the significant ion suppression EtG/EtS urine analysis typically suffers from
More informationFOOD PESTICIDE RESIDUES LABORATORY REFERENCE NO INSPECTOR DATE
FOOD PESTICIDE RESIDUES LABORATORY REFERENCE NO INSPECTOR DATE TYPES OF PESTICIDE ANALYSES: OrganoChlorine Pesticides OrganoPhosphorus Pesticides Nitrogen Containing Pesticides Carbamates Herbicides Standard
More informationPuriTox Aflatoxin Product Code: P25
PuriTox Aflatoxin Product Code: P25 Solid phase clean-up columns for use in conjunction with ELISAs or HPLC. For in vitro use only. P25/V9/01.04.16 www.r-biopharm.com 2 Contents Page Test Principle...4
More informationMaximizing Sample Recovery on the ACCQPrep HP125
Maximizing Sample Recovery on the ACCQPrep HP125 Chromatography Technical Note TN42 Overview This technical note is to investigate the sample recovery and injection efficiency of different methods of injection
More informationFast and Comprehensive 2-Dimensional Liquid Chromatography of Block Copolymers through Temperature Gradient Driven Trapping
Fast and Comprehensive 2-Dimensional Liquid Chromatography of Block Copolymers through Temperature Gradient Driven Trapping International Symposium on GPC/SEC and related techniques Bastiaan Staal & Joshua
More informationErstellt mit einer Testversion von PDF Annotator - FLUIDIC & OPTICAL PRODUCTS AND INFORMATION
Erstellt mit einer Testversion von PDF Annotator - www.pdfannotator.de FLUIDIC & OPTICAL PRODUCTS AND INFORMATION 156 Column Hardware We offer an extensive line of HPLC and UHPLC Column Hardware that has
More informationGas Chromatography Assignment Chem 543/443
Gas Chromatography Assignment Chem 543/443 1. Introduction Capillary gas chromatography (GC) is one of the most popular analytical techniques used in today s research. Its popularity is mainly due to efficient
More informationAutomatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures
Automatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures Thomas E. Wheat, Amanda B. Dlugasch, and Patricia R. McConville Waters Corporation IFPAC January 26, 2016 2016 Waters
More informationMassPREP On-Line Desalting Cartridge
CONTENTS I. INTRODUCTION II. INSTALLING THE MASSPREP ON-LINE DESALTING CARTRIDGE INTO THE SENTRY.1 X 1 MM GUARD COLUMN HOLDER III. RECOMMENDED LC/MS SYSTEM CONFIGURATION TO MINIMIZE MS SOURCE CONTAMINATION
More informationDIDANOSINE ORAL POWDER Final text for addition to The International Pharmacopoeia
Document QAS/06.175(A)FINAL August 2007 DIDANOSINE ORAL POWDER Final text for addition to The International Pharmacopoeia This monograph was adopted at the 41 st WHO Expert Committee on Specifications
More informationGas Filtration Catalogue
Gas Filtration Catalogue 2 SHIMADZU GAS FILTRATION CATALOGUE ABOUT SUPER CLEAN GAS FILTERS Cartridge systems make changing gas filters quick and easy. A base plate allows cartridges to be exchanged without
More informationTHE REGIS PINKERTON ISRP HPLC COLUMN. Its Nature, Care, and Use
THE REGIS PINKERTON ISRP HPLC COLUMN Its Nature, Care, and Use The Regis Pinkerton ISRP HPLC Column Its Nature, Care, and Use TABLE OF CONTENTS Page I. The Nature of the Pinkerton Internal-Surface Reverse
More informationEnhanced biodegradation of carbamazepine after UV/H 2 O 2 advanced. oxidation
Supporting Information for the Environmental Science and Technology article: Enhanced biodegradation of carbamazepine after UV/H 2 O 2 advanced oxidation Prepared for submission to Environmental Science
More informationCorn Syrup Analysis E-1a- 1. ACETALDEHYDE and ISOVALERALDEHYDE (Gas Chromatography)
Corn Syrup Analysis E-1a- 1 ACETALDEHYDE and ISOVALERALDEHYDE (Gas Chromatography) PRINCIPLE Isovaleraldehyde (IVA) and acetaldehyde are released from the syrup with the aid of dilute phosphoric acid and
More informationEASY-Spray Columns. Guidance for column set up and installation Tips to maximize column lifetime
EASY-Spray Columns Guidance for column set up and installation Tips to maximize column lifetime EASY-Spray Column Tips and Tricks This document provides guidance for Thermo Scientific EASY-Spray column
More informationColumn Packing of Cellufine Sulfate
Column Packing of Cellufine Sulfate Cellufine Sulfate is an affinity chromatography resin designed for the concentration, purification and depyrogenation of virus, viral coat, virus like particles (VLP)
More informationBio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml. Instruction Manual. Catalog # ,
Bio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml Instruction Manual Catalog # 732-4600, 732-4602 Table of Contents Section 1...Introduction...1 Section 2 Section 3 Section 4 Connecting to Bio-Rad
More informationAnalytical Instrument Qualification According to USP <1058>: Requirements and Examples for the Agilent 1290 Infinity LC System
Analytical Instrument Qualification According to USP : Requirements and Examples for the Agilent 1290 Infinity LC System 1) USP : Scope, approach, requirements 2) Qualification Examples for
More informationFINAL REPORT. Participants in the reported tasks 1. Ravi Subramanian 2. Manoranjan Misra 3. Kent Hoekman
FINAL REPORT Subtask 2.2.1: Solar thermal hydrogen production Duration of the Project: October 01 2009 - Dec 31, 2010 Date of Final Report Submission: January 24, 2011 Primary Lead contact Vaidyanathan
More informationUHPLC Separation of Triazine Herbicides at Elevated Temperature
Application ote: 453 UPLC Separation of Triazine erbicides at Elevated Temperature Dave Thomas, Thermo Fisher Scientific, San Jose, CA USA Key Words Accela UPLC Environmental Analysis erbicides igh Temperature
More informationSeparate and Quantify Rituximab Aggregates and Fragments with High-Resolution SEC
Separate and Quantify Rituximab Aggregates and Fragments with High-Resolution SEC The Agilent 126 Infinity Bio-Inert Quaternary LC System and the AdvanceBio SEC 3Å, 2.7 µm Column Application Note Biologics
More informationGas Chromatography SSI-GC-003
Gas Chromatography SSI-GC-003 Trace Oxygenated Hydrocarbons in Liquid Hydrocarbon Streams by Multidimensional GC Introduction It has been widely accepted that oxygenates are related to corrosion and fouling
More informationInstructions for Capillary Electrophoresis Peptide Analysis Kit
Instructions for Capillary Electrophoresis Peptide Analysis Kit Catalog Number 148-4110 For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) Table of
More informationEffective Scaling of Preparative Separations Using Axial Compression Columns
Effective Scaling of Preparative Separations Using Axial Compression Columns Peter Rahn, Gareth Friedlander, Graham Osborn, and Emmet Welch Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA Introduction
More informationCapillary GC Troubleshooting: a Practical Approach
Capillary GC Troubleshooting: a Practical Approach Katherine Stenerson Gas Separations R&D Supelco, Supelco Park, Bellefonte, PA 16823 1999 Sigma-Aldrich Co. Outline Basic Troubleshooting Strategy Preventing
More informationDetermination of Diesel Range Organics (DROs) Using SPE and GC/FID by Method 8015D*
Determination of Diesel Range Organics (DROs) Using SPE and GC/FID by Method 8015D* UCT Products: ECUNIPAH (ENVIRO-CLEAN Universal PAH/DRO 2000 mg/83 ml cartridge) Method Summary Method 8015D may be used
More informationDACLATASVIR TABLETS (DACLATASVIRI COMPRESSI) Proposal for The International Pharmacopoeia. (May 2018)
May 2018 Draft for comment 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 DACLATASVIR TABLETS (DACLATASVIRI COMPRESSI) Proposal for The International
More informationMACHEREY-NAGEL. CHROMABOND Flash cartridges. Chromatography. Solutions for preparative purifications. n Resolution n Quality n Safety.
MACHEREY-NAGEL CHROMABOND Flash cartridges Chromatography Solutions for preparative purifications n Resolution n Quality n Safety Table of contents MACHEREY-NAGEL products for flash chromatography Contents
More informationMPLC. Simply Efficient! Watchers Flash Flash Chromatography by ISU. Ver
MPLC Simply Efficient! Watchers Flash Flash Chromatography by ISU Ver. 05-11 MPLC Watchers Flash Cartridges - For performance and simplicity of use - Fast flash technology - Direct dry loading feature
More informationColumn Packing of Cellufine A-500 ion exchange chromatography resin
Column Packing A-500 Column Packing of Cellufine A-500 ion exchange chromatography resin the column inlet. For large columns with a slurry Cellufine A-500 is a conventional weak anion exchange resin which
More informationModule 16: Gel filtration: Principle, Methodology & applications. Dr. Savita Yadav Professor Department of Biophysics AIIMS, New Delhi
PAPER 9: TECHNIQUES USED IN MOLECULAR BIOPHYSICS I Module 16: Gel filtration: Principle, Methodology & applications Dr. Savita Yadav Professor Department of Biophysics AIIMS, New Delhi Module 16 Gel filtration:
More informationPLC Purification Systems
PLC Purification Systems Preparative HPLC, flash, and CPC in one compact purification system LARGE CAPACITY HIGH VERSATILITY COMPACT FOOTPRINT One system for many columns All the features you need in one
More informationcolumnsi o n P a c C S 1 6 C a t i o n - E x c h a n g e C o l u m n
columnsi o n P a c C S 6 C a t i o n - E x c h a n g e C o l u m n The IonPac CS6 cation-exchange column is designed for the determination of disparate concentration ratios of sodium and ammonium (up to,:)
More informationSTANDARD OPERATING PROCEDURES
PAGE: 1 of 5 CONTENTS 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING, AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationNew Developments in Capillary Ion Chromatography Systems with Electrochemical Detection and Their Applications
New Developments in Capillary Ion Chromatography Systems with Electrochemical Detection and Their Applications Jun Cheng, Petr Jandik, Chris Pohl, and Jason Wood Thermo Fisher Scientific, Sunnyvale, CA,
More information