20 tests; option of manual use or walk-away automation with UNIQUE PLUS. UNIQUE PLUS. UNIQUE PLUS. UNIQUE PLUS.

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1 TECRA UNIQUE PLUS UNIQUE PLUS offers affordable automated pathogen screening with true walk-away convenience and fast results. Tests currently available: Salmonella Listeria Staphylococcal Enterotoxins Campylobacter TECRA UNIQUE UNIQUE tests are simple and extremely fast. Test reagents are provided in a ready-to-use module and an antibodycoated dipstick is used to capture the target organism. The presence of the organism is indicated by a purple coloured zone on the stick. UNIQUE Listeria For the detection of Listeria spp. in food and environmental samples in as little as 32 hours. UNIQUE Salmonella LISUNQ20 LISUNQ40 SALUNQ20 SALUNQ40 UNIQUE Staphylococcal Enterotoxins SETUNQ20 UNIQUE Campylobacter CAMUNQ20 TECRA PLUSLINK Software UNQLINK01 40 tests; option of manual use or walk-away automation with For the detection of Salmonella spp. in food, feed, and environmental samples in less than 22 hours. 40 tests; option of manual use or walk-away automation with For the detection of pre-formed SET in food and food-related samples within 4 hours; or to detect the production of SET after overnight enrichment of samples containing enterotoxigenic Staphylococcus spp. For the detection of Campylobacter spp. in food and environmental samples in 42 hours. Software for the download of data from the UNIQUE PLUS instrument to a computer. 09/05 page 1

2 TECRA VISUAL IMMUNOASSAY (VIA ) KITS These kits provide rapid, convenient, specific screening tests. Results are determined either visually, by assessing the development of a green colour for positive results, or by using a microplate reader. Bacillus Diarrhoeal Enterotoxin BDEVIA48 Campylobacter CAMVIA96 CAMVIA48 For the detection of pre-formed BDE in food and food-related samples within 4 hours; or to detect the production of BDE after overnight enrichment of samples containing enterotoxigenic Bacilllus spp. For the detection of Campylobacter spp. in food and environmental E. coli O157 Listeria ECOVIA96 ECOVIA48 LISVIA96 LISVIA48 LISVIA30 For the detection of E. coli O157 including O157:H7 and other O157 EHEC strains in food. For the detection of Listeria spp. in food and environmental Pseudomonas PSDVIA96 For the detection of Pseudomonas spp. in cosmetics, pharmaceuticals, food, water and environmental Salmonella SALVIA96 SALVIA48 SALVIA30 For the detection of Salmonella spp. in food and environmental Salmonella ULTIMA SALULT96 SALULT30 Staphylococcal enterotoxins SETVIA96 SETVIA48 Staphylococcal enterotoxin I.D. SIDVIA72 Staphylococcus aureus STAVIA96 An advanced Visual Immunoassay for the detection of Salmonella spp. in food and environmental A single enrichment protocol for all foods, with no M-broth or secondary enrichment. For the detection of pre-formed SET in food and food-related samples within 4 hours; or to detect the production of SET after overnight enrichment of samples containing enterotoxigenic Staphylococcus spp. For the identification of the Staphylococcal Enterotoxins A,B,C,D and E in food and food-related For the detection of Staphylococcus aureus in food, cosmetics, pharmaceutical products and environmental 09/05 page 2

3 BIOCHEMICAL ID TEST KITS Gram Negative Biochemical ID Kit GNID30 Used to confirm the identity of Enterobacteriaceae and other non fastidious, oxidase negative, Gram negative bacilli, such as Salmonella spp., isolated from food and environmental sources. Listeria Biochemical ID Kit LISID10 Identification Support Systems TISS01 LISPR/GNPR Used to confirm the identity of Listeria spp., isolated from food and environmental Software (version ) for use with both ID Test Kits (PC only, Windows 95 or later) Profile Registers - Booklets containing condensed information on the most commonly encountered organisms. (The TISS01 software contains a more comprehensive database.) TECRA ALLERGEN VISUAL IMMUNOASSAY (VIA ) KITS Egg Peanut EGQVIA96 PNQVIA96 For the detection and measurement of egg in food and food-related For the detection and measurement of peanut in food and foodrelated SWABBING PRODUCTS* ENVIROSWAB ENVSWB25 ENVSWB500 An environmental swab that provides improved recovery of microorganisms, specifically designed for use in the food industry. Dry ENVIROSWAB ENVSWB25D A non-sterile, dry environmental swab designed for use in the food industry. Ideal for use with the Allergen VIA kits. * For more information about the full Biotrace range of swabbing products, please go to TECRA VITAMIN ASSAYS Folate FOLQCA96 A rapid and specific competitive assay for the quantitative detection of Folate (Folic Acid) in food. 09/05 page 3

4 OTHER TECRA PRODUCTS Filter Sample Bags FSTBAG100 Used for the pre-enrichment of meat samples prior to testing with UNIQUE SALMONELLA, UNIQUE LISTERIA, TECRA E. COLI O157 VIA or TECRA CAMPYLOBACTER VIA. Meat Pack MEATPK20 Used for the pre-enrichment of meat samples prior to testing with UNIQUE SALMONELLA. TECRA TUBES System CSNTTS960 RSNTTSG10 TSNTTS960 Used in conjunction with TECRA VIA kits; 1.1mL tubes presented in a 96-well format to facilitate the processing and transfer of Bulk caps for TECRA TUBES System, 960 caps per bag, sufficient for 10 racks. Complete system of 10 racks, each rack containing 96 tubes. Bulk tubes for TECRA TUBES System, 960 tubes per bag, sufficient for 10 racks. MEDIA & MEDIA SUPPLEMENTS Buffered Peptone Water BPWMED500 BPWMED2000 Used as a pre-enrichment medium with a number of different enrichment protocols when testing samples for Salmonella spp. with the TECRA SALMONELLA VIA and SALMONELLA ULTIMA kits. This medium aids the resuscitation of sub-lethally damaged cells. Fraser Broth FRBMED500 For use, after addition of TECRA Fraser Broth Supplement, as a secondary selective enrichment broth for the isolation of Listeria spp. from food and environmental samples; also used as an enrichment medium when testing for Listeria spp., including L. monocytogenes, with the TECRA LISTERIA VIA kit. Fraser Broth Supplement FRBSUP010 For use with Fraser Broth FRBMED500. Imbentin Supplement IMBSUP50 IMBSUP1000 IMBSUP2000 Iodine/Iodide Solution IISSUP250 For use in the pre-enrichment of meat samples prior to testing with UNIQUE SALMONELLA, TECRA E. COLI O157 VIA or SALMONELLA VIA. For use with Tetrathionate Broth Base TTB(B)MED /05 page 4

5 MEDIA & MEDIA SUPPLEMENTS (cont d) Lactose Broth M Broth TLBMED500 For the detection of coliform bacteria by the MPN procedure and for the pre-enrichment of samples for Salmonella testing; also used as part of a Salmonella enrichment protocol, when testing raw foods and high microbial load products with the TECRA SALMONELLA VIA kit. TMBMED500 Modified Buffered Peptone Water MBPMED500 MBPMED2000 Modified Letheen Broth MLBMED500 Rappaport-Vassiliadis R10 Broth RVRMED500 Rappaport-Vassiliadis Soy Peptone Broth RVSMED500 TECRA Buffered Listeria Enrichment Broth BLEMED500 TECRA Campylobacter Enrichment Broth Base TCE(B)MED500 Used as a post-enrichment broth, as part of a Salmonella enrichment protocol, when testing samples with the TECRA SALMONELLA VIA kit. Used as a pre-enrichment medium when screening samples for Salmonella spp. with the TECRA UNIQUE SALMONELLA. This medium aids the resuscitation of sub-lethally damaged cells. Based on the FDA BAM (7th Edition) formulation and designed to inactivate preservative agents. Used as an enrichment medium when testing water, cosmetics and pharmaceutical products for Pseudomonas spp. and/or Staphylococcus aureus with the TECRA STAPHYLOCOCCUS AUREUS VIA and TECRA PSEUDOMONAS VIA kits. A selective enrichment broth for the isolation and cultivation of Salmonella spp. from food and environmental samples; also used as part of a Salmonella enrichment protocol when testing for Salmonella spp. with the TECRA SALMONELLA VIA and SALMONELLA ULTIMA kits. A selective enrichment broth for the isolation and cultivation of Salmonella spp. from food and environmental samples; also used as part of a Salmonella enrichment protocol when testing for Salmonella spp. with the TECRA SALMONELLA VIA and SALMONELLA ULTIMA [AFNOR] kit. For use, after addition of TECRA Listeria Enrichment Supplement, as a selective enrichment medium when screening for Listeria spp., including L. monocytogenes, with the TECRA UNIQUE LISTERIA. For the selective enrichment of Campylobacter spp. in food and environmental samples prior to testing with the TECRA CAMPLYOBACTER VIA kit. Selective agents are supplied separately as TECRA Campylobacter Selective Supplement. This medium also requires ferrous sulfate heptahydrate to be added separately. 09/05 page 5

6 MEDIA & MEDIA SUPPLEMENTS (cont d) TECRA Campylobacter Selective Supplement TCESUP010 TECRA Listeria Enrichment Broth TLEMED500 TECRA Listeria Enrichment Broth Base TLE(B)MED500 TECRA Listeria Enrichment Supplement LISSUP20 TECRA Listeria Selective Supplement TLESUP010 TECRA Modified EC Broth MECMED500 TECRA Pseudomonas Enrichment Broth Base TPE(B)MED500 TECRA Pseudomonas Selective Supplement TPESUP010 TECRA Salmonella Supplement SALSUP250 SALSUP500 TECRA Staphylococcus Growth Medium TSGMED500 For the selective enrichment of Campylobacter spp. - to be added to the TECRA Campylobacter Enrichment Broth Base. For the selective enrichment of Listeria spp. prior to testing with the TECRA LISTERIA VIA kit. For the selective enrichment of Listeria spp. in food and environmental samples prior to testing with the TECRA LISTERIA VIA kit. Selective agents are supplied separately as TECRA Listeria Selective Supplement. For the selective enrichment of Listeria spp. - to be added to the TECRA Buffered Listeria Enrichment Broth, used with TECRA UNIQUE LISTERIA. For the selective enrichment of Listeria spp. - to be added to the TECRA Listeria Enrichment Broth Base, used with the TECRA LISTERIA VIA kit. Specially formulated for the enrichment of Enterohaemorrhagic E. coli, in particular E.coli O157, in food and food-related samples, prior to testing using the TECRA E. COLI O157 VIA kit. This medium requires Sodium Novobiocin to be added separately. For the selective enrichment of Pseudomonas spp. prior to testing with the TECRA PSEUDOMONAS VIA kit. Selective agents are supplied separately as TECRA Pseudomonas Selective Supplement. For the selective enrichment of Pseudomonas spp. - to be added to the TECRA Pseudomonas Enrichment Broth Base. Added to Modified Buffered Peptone Water prior to testing raw flesh foods, animal feeds and raw whole egg for Salmonella spp. using TECRA UNIQUE SALMONELLA. For the selective enrichment of samples prior to testing for S. aureus or enterotoxin-producing Staphylococci with the TECRA STAPHYLOCOCCUS AUREUS VIA kit or STAPHYLOCOCCAL ENTEROTOXIN VIA and TECRA UNIQUE STAPHYLOCOCCAL ENTEROTOXIN kits respectively. 09/05 page 6

7 MEDIA & MEDIA SUPPLEMENTS (cont d) Tetrathionate Broth Base TTB(B)MED500 Tryptone Soy Broth TSBMED500 University of Vermont Medium Used with added Iodine/Iodide Solution, as a selective enrichment medium for the isolation of Salmonella spp. in clinical specimens and food, dairy and environmental samples; also used as part of a Salmonella enrichment protocol, when testing samples with the TECRA SALMONELLA VIA kit. For some food samples, it is the preferred pre-enrichment media when using the TECRA SALMONELLA VIA kit. A general purpose enrichment medium for the growth of bacteria and fungi from clinical and non-clinical samples; also used (with 4% Tween added) as an enrichment medium for Staphylococcus aureus and Pseudomonas spp. using the TECRA STAPHYLOCOCCUS AUREUS VIA or PSEUDOMONAS VIA kits respectively. UVMMED500 For the selective enrichment of Listeria spp., including L. monocytogenes; also used as an enrichment medium when testing for Listeria spp. in meat and meat products (excluding raw ground chuck) and environmental samples with the TECRA LISTERIA VIA kit. READY-TO- MEDIA* MegaReg 25 Buffered Peptone Water USDA-25BPW TECRA Ready-to-use M Broth Screw cap bottles filled with 25mL Buffered Peptone Water, with a demarcation at 15mL so that USDA recommended procedures for carcass sampling for Salmonella can be performed easily. TMBRTU100 Tubes containing 10mL M Broth, to reduce preparatory work for customers using the TECRA SALMONELLA VIA kit. * For more information about the full Biotrace range of Ready-to-use Media, please go to TECRA International Pty Ltd A member of the Biotrace International Plc Group of Companies 13 Rodborough Road, Frenchs Forest NSW 2086, Australia Tel: Fax: enquiries@tecra.net /05 page 7

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