OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : :03:55 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: n swine fever Address of laboratory: Avda Puerta de Hierro s/n Madrid SPAIN Tel.: Fax: address: jmvizcaino@visavet.ucm.es Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof. José Manuel Sánchez-Vizcaíno Name (including Title and Position) of OIE Reference Expert: Prof. José Manuel Sánchez-Vizcaíno Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA indirecto (OIE) Sí ELISA comercial Sí Inmunoblotting Sí 0 50 Inmunocromatografía No Direct diagnostic tests Nationally Internationally Aislamiento y hemoadsorción en macrófagos alveolares porcinos (MAP) Sí 0 2 Inmunofluorescencia directa Sí 0 20 PCR convencional Sí PCR a tiempo real (sonda Taqman) Sí ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2014
3 OIE Reference Laboratory Reports Activities,
4 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Antígeno PPA (vp72) ELISA Suministrado - 1 ml 1 Control positivo, negativo y límite ELISA Suministrado - 0,5 ml 1 Kit extracción DNA PCR Suministrado Master Mix probe KAPA BIOSYSTEM PCR Suministrado Primers PCR Suministrado - 0,5 ml 1 Sonda PCR Suministrado 0,5 ml 1 4 OIE Reference Laboratory Reports Activities, 2014
5 Kit comercial ELISA (detección de anticuerpos) ELISA Suministrado 1 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) ELISA indirecto Sánchez-Vizcaíno, J. M., Tabares, E., Salvador, E., & Ordás, A. (1982). Comparative studies of two antigens for the use in the indirect Elisa test for the detection of ASF antibodies. In EUR 8466 EN, Proceedings of CEC/FAO Research Seminar, Sardinia (pp ). Sánchez Vizcaíno, J.M; Crowther, J; Wardley, R. (1983). A collaborative study on the use of ELISA in the diagnosis of ASF. n swine Fever. CEC. Eur Immunoblotting assay Pastor MJ, Laviada MD, Sanchez-Vizcaino JM, Escribano JM. Detection of n swine fever virus antibodies by immunoblotting assay. Canadian Journal of Veterinary Research 1989;53(1): ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? OIE Reference Laboratory Reports Activities,
6 Name of OIE Member Country seeking assistance Date (month) No. samples received for provision of diagnostic support No. samples received for provision of confirmatory diagnoses RUSSIA Marzo KENYA Abril Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided RUSSIA Curso de formación en diagnóstico de PPA Asistencia in situ (Rusia) y seguimiento a distancia KENYA Curso de formación en diagnóstico PPA Asistencia in situ (Kenia) y seguimiento a distancia ITALY Erradicación de PPA en Cerdeña: aspectos técnicos y estrategia Asistencia in situ (Cerdeña) y a distancia KENYA Formación en manejo de muestras, trazabilidad y otros procedimientos laboratoriales Visita de una semana de dos miembros de Kabete a nuestro laboratorio en Madrid ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) ASFORCE 3 años Esfuerzo de investigación focalizada en PPA Proyecto o con 18 instituciones asociadas Development of n swine fever diagnostic 3 años Desarrollo de técnicas diagnósticas de PPA adaptadas al Iowa State University, Kansas State University 6 OIE Reference Laboratory Reports Activities, 2014
7 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 6 1.Sánchez-Vizcaíno, JM., Mur, L., Penrith, ML. New insights of the role of ticks in n swine fever epidemiology. Aceptada para su publicación en Rev. sci. tech. Off. int. Epiz. 2.Mur L., Igolkin A., Varentsova A., Pershin A., Remyga S., Shevchenko I., Zhukov I., Sánchez-Vizcaíno JM. Detection of n Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control. Transboundary and Emerging Diseases. 3.Sanchez-Vizcaino JM., Mur L., Gomez-Villamandos JC., Carrasco L. "An Update on the Epidemiology and Pathology of n Swine Fever". Journal of Comparative Pathology. In press (Online 11/2014). 4.Mur L., Atzeni M., Martínez-López B., Feliziani F., Rolesu S., Sanchez-Vizcaino JM. "Thirty-Five-Year Presence of n Swine Fever in Sardinia: History, Evolution and Risk Factors for Disease Maintenance". Transbound Emerg Dis. 5.De la Torre A, Bosch J, Iglesias I, Muñoz MJ, Mur L, Martínez-López B, Martínez M, Sánchez-Vizcaíno JM. "Assessing the Risk of n Swine Fever Introduction into the an Union by Wild Boar". Transbound Emerg Dis. 6.Mur L., Martínez-López B., Costard S., de la Torre A., Jones BA., Martínez M., Sánchez-Vizcaíno F., Muñoz MJ., Pfeiffer DU., Sánchez-Vizcaíno JM., Wieland B. "Modular framework to assess the risk of n swine fever virus entry into the an Union". BMC Vet Res. 10(1):145 b) International conferences: 14 1.Mur, L., Martínez-López, B., Sanchez-Vizcaino, JM., Epidemiology of ASF in ern and the risk derived from it, Key note lecture. 8th Annual Meeting EPIZONE, September Copenhague, Dinamarca. 2.Mur, L., Fernández, E., Atzeni, M., Rolesu, S., Feliziani, F., Sánchez-Vizcaíno, JM., Martínez-López, B., Combination of simulation and statistical modeling to identify factors contributing to ASF endemicity in Sardinia. 8th Annual Meeting EPIZONE, September Copenhague, Dinamarca. 3.Feliziani, F., Mur, L., Iscaro, C., Bandino, E., Liciardi, M., Oggiano, A., Ruiu, A., Rolesu, S., Sánchez Vizcaíno, J.M., Indagine sulla presenza di Ornithodoros erraticus in Sardegna: risultati preliminari. V Workshop Nazionale di Virologia Veterinaria June Teramo, Italia. 4.Giménez-Lirola, LG., Mur, L., Rivera, B., Lizano, S., Goodell, C., Rowland, R., Mogler, M., Harris, DL., Gallardo, C., Arias, M., Sánchez-Vizcaíno, JM., Zimmerman, J. (2014). Simultaneous detection of n swine fever virus antibodies in serum and oral fluid using a recombinant p30 antibody ELISA. 57th AAVLD/118th USAHA Meeting. Kansas City, Missouri,16-22/10 5.Sánchez-Vizcaíno, JM (2014). Contingency Policy Making in the EU n swine fever (ASF) Policy Event- Lessons learnt from and policy challenges for Asia. Beijing, PR China. 17/ Sánchez-Vicaíno, JM (2014). Key points for the prevention and control of ASF FP7 LinkTADs WP4 2nd OIE Reference Laboratory Reports Activities,
8 Workshop on Vaccine and Diagnostic Technology Development on Transboundary Animal Diseases and Zoonoses. Shangai, China. 17/10 7.Sánchez-Vizcaíno, JM (2014). Chairman of the Special topic Session. ASF 8th Annual Meeting of EPIZONE. Copenhague, Dinamarca /9. 8.Giménez-Lirola, LG., Mur, L., Rivera, B., Wang C., Rowland, R., Harris, DL., Gallardo, C., Arias, M., Zimmenrman, J., Sánchez-Vizcaíno, JM (2014). Oral presentation: Multiplex Luminex assay for detection of antibodies against three major proteins of ASFV 23rd International Pig Veterinary Society (IPVS) Congress. Cancun, Quintana Roo, Mexico. 8/6 9. Giménez-Lirola LG., Mur L., Rivera B., Lizano S., Goodell C., Rowland R., Mogler M., Harris DL., Gallardo C., Arias M., Sánchez-Vizcaíno JM., Zimmerman J. (2014). Simultaneous detection of n swine fever virus antibodies in serum and oral fluid using a recombinant p30 antibody ELISA. 57th AAVLD/118th USAHA Meeting, Kansas City, Missouri, USA Octubre. 10. Giménez-Lirola LG., Mur L., Rivera B., Wang C., Rowland R., Harris DL., Gallardo C., Arias M., Zimmerman J., Sánchez-Vizcaíno JM. (2014). Multiplex Luminex assay for detection of antibodies against three major proteins of ASFV. 23rd International Pig Veterinary Society (IPVS) Congress. Cancún, México 8-11 Junio. 11.Giménez-Lirola LG., Mur L., Rivera B., Wang C., Lizano S., Goodell C., Rowland R., Harris DL., Gallardo C., Arias M., Sánchez-Vizcaíno JM., Zimmerman J. (2014). The n swine fever virus rp30 ELISA detects antibody in serum and/or oral fluid specimens. 23rd International Pig Veterinary Society (IPVS) Congress. Cancún, México 8-11 Junio. 12.Sánchez-Vizcaíno JM. (2014). n Swine Fever: New challenges and measures to prevent its spred 82nd General Session World Assembly of Delegates of the World Organization for Animal Health (OIE).Paris de Mayo. 13.Sánchez-Vizcaíno, JM (2014). OIE Ad hoc group on n Swine Fever. Paris, Francia Abril. 14.Sánchez-Vizcaíno, JM (2014). Mesa redonda sobre la erradicación de la peste porcina africana. La erradicación de la peste porcina africana. International Veterinary Congress. Russian Veterinary Association. The city of Kazan, Republic of Tatarstan, Russia Abril 15.Sánchez-Vizcaíno, JM (2014). Conferencia internacional Peste Porcina na, SZIE Faculty of Veterinary Science, Budapest, 5 Marzo. 16. Sánchez-Vizcaíno JM. (2014). n Swine fever (ASF); what can we do?. n Swine Fever Session en el Workshop on Laboratory Diagnosis of n and Classical Swine Fever (ASF and CSF) del Centro de Investigación en Sanidad Animal. CISA-INIA. Madrid 2-3 Junio. c) National conferences: 2 1.Sánchez-Vizcaíno JM. (2014). Situación actual y perspectivas de futuro de la Peste Porcina na en Europa. Conferencia en la Jornada de Virología en Sanidad Animal y Salud Pública de la Academia de Veterinaria de la Región de Murcia. Murcia 5 Junio. 2.Sánchez-Vizcaíno JM. (2014). Peste porcina africana Máster en virología de la Universidad Complutense de Madrid. Madrid. 5 de Mayo d) Other: (Provide website address or link to appropriate information) 1 Tesis doctoral(mención a: Nuevas estrategias para la prevención y control de la peste porcina africana. Lina Mur Gil. Facultad de Veterinaria. Universidad Complutense. Octubre OIE Reference Laboratory Reports Activities, 2014
9 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 2 c) Hands-on training courses: 2 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country a Rusia 2 b Rusia 2 c Rusia 2 a Kenia 2 b Kenia 2 c Kenia 2 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Certificado ISO17025.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited 817/LE1410 Accreditation body ENAC OIE Reference Laboratory Reports Activities,
10 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? No 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Workshop: n swine fever in wild boar 3/2014 Uppsala, Suecia Orador What makes ASF different?; Main risk point control General session World Assembly of Delegates of the World Organization for Animal Health 5/2014 Paris, Francia Orador n swine fever: New challenges and measures to prevent its spread Workshop on Laboratory Diagnosis of n and Classical Swine Fever 6/2014 Madrid, España Orador n Swine Fever; what can we do? Workshop on Vaccine and Diagnostic Technology Development on Transboundary Animal Diseases and Zoonosis 10/2014 Shangai, China Orador Key points for the prevention and control of ASF Pig day 10/2014 Sassari, Cerdeña, Italia Orador Implications of widespread diseases on the an pig industry ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 10 OIE Reference Laboratory Reports Activities, 2014
11 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? No 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories ASFORCE Esfuerzo de investigación focalizada en PPA Laboratorio de Referencia de la OIE para PPA (Pirbright) UE REFERENCE LABORATORY Esfuerzo de investigación focalizada en PPA CISA (Madrid) ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 OIE Reference Laboratory Reports Activities,
12 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries Evaluación de pruebas diagnósticas (lateral flow devices) para diagnóstico en paralelo de CSF y ASF en el proyecto europeo RAPIDIA 12 Evaluación de la capacidad diagnóstica de PPA 1 Evaluación de la capacidad diagnóstica de PPA 1 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Twinning training Vladimir, Rusia Diagnóstico PPA Twinning training Kabete, Kenya Diagnóstico PPA 25. Additional comments regarding your report: Nuestros expertos están siempre a disposición de la OIE 12 OIE Reference Laboratory Reports Activities, 2014
OIE Reference Laboratory Reports Activities
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