INTEGRATED DNA TECHNOLOGIES. Products & Services
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1 INTEGRATED DNA TECHNOLOGIES THE CUSTOM BIOLOGY COMPANY Products & Services
2 CUSTOM DNA & RNA Custom DNA Synthesis Synthesis Scale Length (Tubes) (Plates) 25 nmole bases.09/base.08/base 100 nmole bases.13/base.10/base 250 nmole bases.25/base.23/base 1 µmole bases.50/base.45/base 5 µmole 5-50 bases 7.50/base Inquire 10 µmole 5-50 bases 14.50/base Inquire IDT offers many analytical and preparative services for oligos including: All oligos QC tested by mass spectrometry LabReady Oligo Service shipped in IDTE at 100 µm concentration Standard mixed-base sites Custom analytical services Visit for details. SameDay Oligo Service HOTplates.57/base Shipped same business day 2 OD minimum yield guarantee Sufficient for >250 PCR reactions bases Delivered lyophilized in tubes Deprotected and desalted Unmodified.08/base Expedite Fee Shipped next business day Normalized to 2-10 nmoles/well or full synthesis yield bases Shipped in 300 µl V-bottom plates Unmodified Minimum of 48 oligos per plate ULTRAmers TM Long Oligo Synthesis ( bases) Product Purification Guaranteed Yield 4-nmole ULTRAmer DNA Oligo Standard Desalt 4 nmoles.35/base 20-nmole ULTRAmer DNA Oligo Standard Desalt 20 nmoles.70/base PAGE Purification for ULTRAmer PAGE Purification Inquire nmole ULTRAmer Plate Oligo Standard Desalt 4 nmoles/well.18/base See for modifications for Ultramer oligos. Custom RNA Synthesis Product 100 nmole 250 nmole 1 µmole 5 µmole 10 µmole RNA 5-50 bases O-Methyl RNA bases Chimeric DNA bases* Antisense Phosphorothioate bonds Methyl dc bases Na + Salt Exchange
3 MODIFICATIONS & PURIFICATIONS Modifications 100 nmole 250 nmole 1 µmole Phosphorylation 5 Phosphorylation Phosphorylation Attachment Chemistry & Linkers 5 Amino Modifier C Amino Modifier Biotin Biotin Modified Bases DeoxyInosine DeoxyUridine & Int 5-Methyl dc Spacers 5 & Int Spacer & Int Spacer C3 Spacer Fluorophores 5 & 3 6-FAM TM HEX TM TET TM ' TAMRA TM JOE (NHS Ester) Cy Cy Quenchers Iowa Black Black Hole Quencher TM Freedom Dyes IDT has created a class of royalty-free dyes for any purpose, including diagnostic and commercial applications. Our in-house chemistry research and licensing efforts with dye suppliers have enabled us to offer a comprehensive set of Freedom Dyes that span the entire spectrum. Freedom Dye FAM TM MAX TM 550 TYE TM 563 TEX TM 615 TYE TM 665 TYE TM 705 Comparable Dyes FAM TM JOE TM, VIC TM Cy3 TM Texas Red -X Cy5 TM Cy5.5 TM Freedom Dye* 100 nmole 250 nmole 1 µmole 5 MAX TM TYE TM TEX TM TYE TM TYE TM *Most Freedom Dyes are also available for 3 end-labeling. Visit for more information. Additional purification may be required for some modifications. Purification Scale HPLC IE HPLC PAGE RNase-Free HPLC Dual HPLC Dual PAGE & HPLC 100 nmole N/A 250 nmole µmole µmole µmole Rapid HPLC TM Product 100 nmole 250 nmole 100 nmole 250 nmole DNA bases Length bases Length bases.13/base.25/base Rapid HPLC TM Purification Minimum Yield: 1 OD Minimum Yield: 4 ODs Expedited purification service for unmodified oligos. Rapid HPLC oligos are shipped next business day. PAGE obtains extremely high purity, especially for unmodified oligos and Ultramers. PAGE purification is strongly recommended for oligos >60 bases in length. For unmodified oligos, purity of >90% is guaranteed. Reverse-Phase HPLC is well suited to purify any modified or unmodified oligo sequence up to 60 bases in length. Ion-exchange HPLC (IE-HPLC) is available to purify long DNA oligos. For unmodified oligos, purity of >85% is guaranteed.
4 GENES & GENE SILENCING Genes Product Yield Length Delivery minigenes Custom Gene Synthesis 2 µg purified plasmid DNA 2 µg purified plasmid DNA Up to 400 base pairs Over 400 base pairs 4-8 business days 8-12 business days flat price.35 base pair Genes can be used in a number of applications including: Protein expression mirna genes Template for in vitro transcription DNA Vaccines and Vectors Gene variants and SNPs Vectors All genes, unless otherwise specified, will be delivered in IDT s proprietary cloning vector, pidtsmart. This vector has been specifically engineered to remove most common restriction endonuclease cleavage sites and does not contain a promoter within the cloning region. If you require restriction sites or promoters for your application, it is important to specify these when ordering the gene of interest by appending the desired restriction sites or promoters to the ends of the desired gene sequence. The default version of pidtsmart contains a kanamycin resistance cassette. An ampicillin version is also available upon request. IDT also offers a pidtblue vector that does contain restriction sites as well as T7 and T3 promoters. However, it is important to note that this is not an expression vector. Additional subcloning will be required for expression. Custom Vector Request IDT has the ability to sub-clone into almost any customer-supplied vector. To start the process, go to IDT s website and complete the Custom Vector Request Sheet or contact IDT Gene Support at genes@idtdna.com. Dicer-Substrate sirna Screening Duplexes (DsiRNAs) Affinity-Purified Tube Duplexes Affinity-Purified Plate Duplexes HPLC-Purified Tube Duplexes* 2 nmole nmole nmole N/A TriFECTa The TriFECTa Kit contains 3 predesigned DsiRNA duplexes, 3 control sequences, and RNase-free duplex buffer to perform RNAi experiments. Ideal for small-scale in vitro applications Dicer-substrate sirnas (DsiRNAs) are chemically synthesized Dicer-substrate duplex sirnas that have increased potency in RNA interference experiments compared to traditional sirnas. They were developed as a collaborative effort between John Rossi (Beckman Research Institute of the City of Hope) and IDT. Dicer-substrate sirnas are designed to be optimally processed by Dicer and show increased potency by engaging this natural processing pathway. Using this approach, sustained knockdown has been regularly achieved using sub-nanomolar concentrations. New design rules specific to DsiRNAs have been developed by IDT and are available only at
5 GENE EXPRESSION PrimeTime Dual-Labeled Probes for qpcr Synthesis Scale Reporter/Quencher 100 nmole 250 nmole 1 µmole 5 6-FAM / ZEN / 3 Iowa Black FQ FAM TM / 3 Iowa Black FQ FAM TM / 3 TAMRA TM FAM TM / 3 BHQ FAM TM / 3 BHQ TET TM / 3 Iowa Black FQ TET TM / 3 BHQ HEX TM / 3 Iowa Black FQ HEX TM / 3 BHQ MAX TM 550 / 3 Iowa Black FQ TYE TM 563 / 3 Iowa Black RQ Cy3 TM / 3 BHQ TEX TM 615 / 3 Iowa Black RQ TYE TM 665 / 3 Iowa Black RQ Cy5 TM / 3 BHQ PrimeTime Mini Probes The PrimeTime Mini qpcr probes are probes available on a small scale. Mini probes are offered with a 5 FAM and the option of a 3 Iowa Black FQ quencher alone or in combination with the internal ZEN quencher. The delivery amount is 0.5 nmoles and the estimated turnaround time is 3-5 days. 5 Reporter Dye Quencher(s) 0.5 nmoles ZEN /Iowa Black FQ FAM Iowa Black The ZEN internal quencher decreases the length between the fluorophore and quencher resulting in: Less background Reduced Cq values Improved precision For more information on ZEN internal quenchers, go to PrimeTime qpcr Assays PrimeTime qpcr Assays consist of a forward primer, a reverse primer, and a hydrolysis probe delivered in a single tube. With the added capability of selecting from multiple dye-quencher combinations, primer-to-probe ratio, and qpcr design parameters, optimizing qpcr reactions is no longer complicated or expensive. PrimeTime qpcr Assays are offered in three different sizes to meet any qpcr experimental need. In addition, for the Standard and XL sizes, selection of dye-quencher combination and primer-to-probe ratio can be specified to meet unique experimental demands. Each Assay ships in 2 to 4 days from order placement. Each oligo undergoes 100% QC by mass spectrometry, and all QC results are provided free of charge on the IDT website. Reactions (20 µl) (FAM TM /ZEN/Iowa Black FQ) (all other dye/quencher combinations) Probe (nmoles) PrimeTime Mini Not Available Primers (nmoles) PrimeTime Standard PrimeTime XL Dye/Quencher Combinations Dye Quencher Mini Standard XL 5 FAM TM ZEN / 3 Iowa Black FQ 5 FAM TM 3 TAMRA TM 5 HEX TM 3 Iowa Black FQ 5 TET TM 3 Iowa Black FQ 5 Cy5 TM 3 Iowa Black RQ Order qpcr assays through IDT s free RealTime PCR design tool or provide custom sequences.
6 cgmp MANUFACTURING & SCITOOLS cgmp Diagnostic Oligo Manufacturing IDT s Clinical and Commercial Manufacturing (CCM) suite is dedicated to the cgmp manufacture of diagnostic oligos. The CCM suite parallels IDT s proven research line which simplifies the transition of oligos and products from research to clinical applications. The CCM suite is ISO 13485:2003 certified, registered with the US Food & Drug Administration (FDA), and is a contract manufacturer for in vitro diagnostic devices (IVDs), analyte-specific reagents (ASRs) and nucleic acid tests (NATs). The CCM service includes dedicated ordering, production, final-fill, and shipping systems that meet the elevated quality standards required for the product s use in clinical or commercial applications. Benefits of IDT cgmp manufacturing Capability to provide 10 nmole to 1 gram final deliverable utilizing dedicated production equipment Three synthesis platforms to meet all of your scale and modification needs Multiple HPLC purification methods are available including IE-HPLC and RP-HPLC Equipment and Process Validations are transparent to customers Validated electronic batch record capability for each Manufacturing Lot (individual syntheses, HPLC fractions, etc.) SciTools IDT offers a number of free design and analysis tools in the online SciTools suite. The suite is comprised of tools for designing assay components as well as determining the properties of any sequence entered. OligoAnalyzer Analyze the physical properties of any oligo sequence PrimerQuest Design primers and probes for PCR and qpcr reactions RealTime PCR Design custom qpcr assays Pre-designed DsiRNA Pre-designed sirna duplexes and TriFECTa Kits RNAi design Design duplexed RNA oligos for RNA interference experiments 454 FusionPrimers Design primers for 454 Sequencing applications UNAFold Secondary structure prediction tool DilutionCalc Calculate volume needed to dilute down to a lower concentration ResuspensionCalc - Calculate how much volume (ul) to resuspend a dry lyophilized oligo Ordering & Customer Care Information IDT offers convenient online ordering: Contact IDT at +32 (0) or eutechsupport@idtdna.com Please use quote #20711 to obtain this pricing. * Products are not tendered pricing. For more information contact your local sales representative: David Hughes UK Sales Manager +44 (0) dhughes@idtdna.com Caron Wilson Technical Sales Specialist +44 (0) cwilson@idtdna.com Shane O Brien Sales Manager, Ireland & Northern UK +353 (0) sobrien@idtdna.com Web chat is available at
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