Product origin. Solfatara volcanic water. Sulfolobus solfataricus. The product: engineered SsoPox variant. T : about 90 C ph: 1 to 5

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2 Product origin Solfatara volcanic water T : about 90 C ph: 1 to 5 Sulfolobus solfataricus Extremophilic Archeon Thermostable enzymes Produce SsoPox protein The product: engineered SsoPox variant PTE (Phosphotriesterase) Lactonases PTE Lactonase

3 Applications PTE activity allows to degradate toxic organophosphates Nerve agents decontamination Insecticides decontamination Lactonase activity Bacterial virulence inhibitor Biofilm formation limitation

4 Bacterial virulence inhibitor Anti-biofilm

5 Mode of action Enzymatic hydrolysis of molecules involved in inter-bacterial communication (i.e quorum sensing) Quorum sensing Used by many bacteria (including pathogens) Mediated mainly by AHL in gram negative Coordinates gene expression according to cell density Regulates virulence & biofilm formation Efficient and stable enzyme (SsoPox) that prevents Virulence factors expression Biofilm formation AHL Lactonase

6 Medical applications Based on the virulence neutralization ability of the product On military Area of Operation Emergency treatment of wounded soldiers Prevention of nosocomial infections e.g. Pseudomonas aeruginosa or Acinetobacter baumanii (a.k.a Iraquibacter) In hospital Treatment of Burned patients Diabetic foot Intensive Care Units devices Drug for treatment for Otitis Pulmonary infection (cystic fibrosis).

7 Medical applications Based on the biofilm formation inhibition capacity of the product Anti-biofilm medical devices (coated enzyme) Catheters Respiratory assists devices Probes Contact lens cleaning Prevents bacterial infections

8 Other applications: Protection against Fire Blight Based on the virulence neutralization ability of the product Anti-fire blight agent Bacteria such as Erwinia can cause plant diseases such as fire blight or bacterial soft rot. The enzyme blocks the quorum sensing of Erwinia and protects the plant. A bio pesticide (no environmental impact)

9 Antibiofouling agent Based on the biofilm formation inhibition capacity of the product Enzyme included in paints to prevent biofouling on Hulls Pipes Membranes

10 Advantages of the product Efficient on antiobiotic-resistant strains Partially restores antiobiotic sensibility of resistant strains Can be use in combination with antibiotics Non Toxic No environmental impact Easy and long-term storage at room temperature

11 Organophosphate decontamination

12 Mode of action Rapid enzymatic hydrolysis of target molecules: 5 to 15 minutes. Subsequent residual products are non toxic. Chemical Warfare Agent (CWA) Sarin VX (CVX, RVX) Soman Tabun Pesticides Parathion Malathion Diazinon Chlopyfiros

13 Decontamination of agricultural pesticides Polluted fields Water (cartridges) Contaminated fruits and vegetables cleaning Agricultural devices and clothes

14 Military & Civilian security Emergency decontamination in case of Terrorist attack (Tokyo, 1995) CWA exposition in Area of Operation After accidental pollution Degradation of CWA stockpiles Of OPs pesticides traces on vegetable and fruits

15 Advantages Easy to use: spray the enzyme Stable: over time and versus temperature variations Non Toxic: skin, mucosal contact & ingestion No environmental impact No secondary pollution Easy decision making

16 Overall product characteristics Hyper thermostability (T m =105 C) Efficient from 5 to 95 C Not toxic No environnemental impact Stability over time Resistant to solvents and detergents Usable as in paintings Competitive price

17 Contact & Informations Dr Claude Escarguel, CEO Pr Eric Chabrière, CSO Mobile: +(33) eric.chabriere@univ-amu.fr Dr Mikael Elias, Scientific supervisor Mobile: +(33) mikael.elias@gmx.fr Gene and Greentech 135, traverse du Gaou-Le brusc Six-fours