Molecular Imager ChemiDoc XRS+ Systems

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1 Imaging Molecular Imager ChemiDoc XRS+ Systems Easy to Use, Reproducible, and Sensitive Solution for Western Blot and Gel Image Analysis

The Trusted Leader in Easy to Use Systems for Reproducible Results For more than two decades, Molecular Imager systems from Bio-Rad have been widely recognized and trusted high-quality imaging

2 The Trusted Leader in Easy to Use Systems for Reproducible Results For more than two decades, Molecular Imager systems from Bio-Rad have been widely recognized and trusted high-quality imaging instruments. Whether your research includes routine imaging of chemiluminescent western blots from cell lysates or purified proteins, SDS-PAGE or PCR products for diagnostics or therapeutic development the ChemiDoc XRS+ imaging system will meet your needs. Designed for ease of use and ability to support a wide array of applications, it is a perfect fit for individual laboratories or multiuser core facilities. Quality standards of engineering and manufacturing make the system adaptable to academic or biopharmaceutical laboratories. Thousands of researchers over the years have published images acquired with the ChemiDoc XRS system in hundreds of journal articles and grant proposals and established a record of reliability and excellence for the system. Key Benefits of the System: n High sensitivity and quantitative images detection with sensitivity comparable to that of film plus the ability to quantify differential sample expression levels n Reproducible acquisition settings can be stored for quick imaging of like samples and to ensure a constant imaging environment n Flexible and easy to use platform perform simple documentation to check for absence or presence of proteins or sophisticated sample analysis to refine antibody concentration or optimize growth conditions of cell lines n Quality optics beautiful, clean images for your laboratory notebook, presentations, and publications n Trusted name with more than 0 years of award-winning life science products, support, and service, Bio-Rad Laboratories is devoted to helping you achieve your research results Application Chemiluminescent western blots Chemifluorescent western blots Fluorescent western blots Chemiluminescent ELISA Colorimetric gels and blots Fluorescent protein gels Fluorescent and colorimetric colony arrays Fluorescence stained DNA and RNA gels ChemiDoc XRS+ System

Green Fluorescent Protein (GFP) Coomassie Brilliant Blue R-0 stain Immun-Star WesternC

System Applications Guide Application Stain/Label/Dye/Method Light Source Filter Blotting

Epi-illumination, white None (colorimetric) Qdot Transillumination, UV 0DF0 Qdot

Transillumination, white None electrophoresis Copper stain Epi-illumination, white None Flamingo

Transillumination, white Clear SYPRO Orange XcitaBlue screen 0BP0 SYPRO Ruby Transillumination,

UV EtBr/SYBR Green electrophoresis GelStar XcitaBlue screen EtBr/SYBR Green Fluorescein

EtBr/SYBR Green Radiant Red stain Transillumination, UV 0BP0 SYBR Green XcitaBlue screen

Red Transillumination, UV 0BP0 Fluorescence Cy XcitaBlue screen EtBr/SYBR Green detection Pro-Q

3 Green Fluorescent Protein (GFP) Coomassie Brilliant Blue R-0 stain Immun-Star WesternC chemiluminescent detection Qdot blot SYPRO Ruby protein gel stain SYBR Safe stain ChemiDoc XRS+ System Applications Guide Application Stain/Label/Dye/Method Light Source Filter Blotting Chemiluminescence None None Chemifluorescence Transillumination, UV 0DF0 Alkaline phosphatase Epi-illumination, white None (colorimetric) Qdot Transillumination, UV 0DF0 Qdot Transillumination, UV 0BP0 X-ray film Transillumination, white None Protein gel Coomassie Blue Transillumination, white None electrophoresis Copper stain Epi-illumination, white None Flamingo fluorescent XcitaBlue screen 0BP0 gel stain NanoOrange XcitaBlue screen 0BP0 Silver stain Transillumination, white Clear SYPRO Orange XcitaBlue screen 0BP0 SYPRO Ruby Transillumination, UV 0BP0 Zinc stain Epi-illumination, white None Nucleic acid Ethidium bromide Transillumination, UV EtBr/SYBR Green electrophoresis GelStar XcitaBlue screen EtBr/SYBR Green Fluorescein Transillumination, UV 0BP0 OliGreen XcitaBlue screen EtBr/SYBR Green PicoGreen XcitaBlue screen EtBr/SYBR Green Radiant Red stain Transillumination, UV 0BP0 SYBR Green XcitaBlue screen EtBr/SYBR Green SYBR Gold XcitaBlue screen EtBr/SYBR Green SYBR Safe XcitaBlue screen 0DF0 Texas Red Transillumination, UV 0BP0 Fluorescence Cy XcitaBlue screen EtBr/SYBR Green detection Pro-Q Emerald 00 Transillumination, UV EtBr/SYBR Green Pro-Q Emerald XcitaBlue screen EtBr/SYBR Green GFP XcitaBlue screen EtBr/SYBR Green FITC XcitaBlue screen EtBr/SYBR Green

00,000 0,000 Limit of Detection of Film and ChemiDoc XRS+ System ChemiDoc XRS+ system X-ray film A C Chemiluminescence, RU 00,000 0,000 00,000 B D 0,000 0 00 0...... 0. Human serum volume, pl X-ray film (00 sec exposure) Quantitative dynamic range and visualization tools.

A variety of visualization tools are provided for sample verification. B, the -D viewer shows the distinct peaks of the most concentrated band as well as the faintest band.

confirming linear data over the entire range of sample detection.

Blots of twofold serial dilutions of human serum were probed with rabbit anti-human transferrin polyclonal antibodies. A /,000 dilution of human serum was used to make the twofold serial dilutions.

4 00,000 0,000 Limit of Detection of Film and ChemiDoc XRS+ System ChemiDoc XRS+ system X-ray film A C Chemiluminescence, RU 00,000 0,000 00,000 B D 0, Human serum volume, pl X-ray film (00 sec exposure) Quantitative dynamic range and visualization tools. A, the ChemiDoc XRS+ system detects the entire range of sample concentration without saturating the most concentrated band, enabling linear quantitation. A variety of visualization tools are provided for sample verification. B, the -D viewer shows the distinct peaks of the most concentrated band as well as the faintest band. C, the lane profiles provide quick comparison of expression levels from each lane. D, the volume regression curve shows a correlation coefficient R of 0. confirming linear data over the entire range of sample detection. ChemiDoc XRS+ system (0 sec exposure) Comparison of chemiluminescent western blot detection on X-ray film and with the ChemiDoc XRS+ system. Blots of twofold serial dilutions of human serum were probed with rabbit anti-human transferrin polyclonal antibodies. A /,000 dilution of human serum was used to make the twofold serial dilutions. A 00 sec exposure on film does not reach the same limit of detection that is reached by a 0 sec exposure in the ChemiDoc XRS+ system. Chemiluminescence Visible Marker Merged Image Quantitative data. Film reached maximum detection limit at 00 sec, but the first two dilutions are saturated. Using the Quantity One -D analysis software -D viewer shows that the tops of the bands are flattened and are no longer quantitative, as there is no way to visualize their peaks and differentiate the exact volume of each. The 0 sec exposure on the ChemiDoc XRS+ system shows that the first two dilutions are not saturated; the peaks are visible on the -D viewer, enabling differentiation of the protein concentrations. Analysis Chemiluminescent sample detection and visible markers. Visible markers are commonly used to monitor any problems with SDS-PAGE and blot transfer. To determine the molecular weights, users trace the pattern of the visible marker from the gel on the blot or film. This adds additional steps and can possibly introduce errors. The ChemiDoc XRS+ system images the chemiluminescent samples and visible markers to provide a digital record; the images are then merged for molecular weight determination. 0 sec 0 sec 0 sec Live acquisition. The ChemiDoc XRS+ system tracks progressive image development, allowing users to visualize the chemiluminescent reaction in real time and capture the image they want. The total acquisition time and the number of images taken during this time can be customized, and each image can be saved. This removes the tricky exposure bracketing often needed even with a single film exposure. Multifluorescent western blot detection of maltose binding protein (MBP) and Profinity exact fusion-tagged MBP bands using Qdot and Qdot fluorescent conjugated secondary antibodies (Invitrogen Corp.), respectively, imaged with the ChemiDoc XRS+ system. The blot was imaged with excitation via UV, and separate images were taken by switching between the SYBR Green (Qdot ) and SYPRO Ruby stain (Qdot ) filters installed in the ChemiDoc XRS+ imager. Multifluorescent western blotting enables detection of two antibody-specific proteins at once, removing the variability involved with two blots or the loss of protein with stripping. For more information, refer to bulletin.

The Molecular Imager ChemiDoc XRS+ system enables direct digital visualization of chemiluminescent western blots to allow quantitation of differential protein expression.

signals. Quality optics engineered for optimal signal-to-noise ratio ensure antibody-specific detection of low-abundance proteins.

A self-contained lighttight enclosure means you don t need a separate darkroom and won t have to bother with scheduling an experiment around darkroom availability.

Acquisition settings can be stored for repeated use during your experiment or project, such as for efficacy studies over a period of time.

colleagues, and they can be printed for documentation in laboratory notebooks. A Chemiluminescent ELISA arrays.

The attributes of high resolution and sensitivity enable the ChemiDoc XRS+ system to image the entire plate (panel A) while resolving each array spot (panel B) with the

B C D The ChemiDoc XRS+ system is flexible and easy to use and supports other detection methods including fluorescence and colorimetry.

The ChemiDoc XRS+ system is the ideal complement to your electrophoresis, purification, and PCR systems, enabling image analysis and documentation of western blots,

5 The Molecular Imager ChemiDoc XRS+ system enables direct digital visualization of chemiluminescent western blots to allow quantitation of differential protein expression. Live image acquisition records real-time development of the chemiluminescent reaction, removing the guesswork often involved with single-event film capture of blot signals. Quality optics engineered for optimal signal-to-noise ratio ensure antibody-specific detection of low-abundance proteins. Automatic digital capture of chemiluminescent blot signals eliminates the chemical use and disposal problems involved with film developing. A self-contained lighttight enclosure means you don t need a separate darkroom and won t have to bother with scheduling an experiment around darkroom availability. Sophisticated analysis features support a variety of samples, including cell lysates and purified proteins. Acquisition settings can be stored for repeated use during your experiment or project, such as for efficacy studies over a period of time. Quality digital images, numerical data, and sample annotations are easily exported for publication in grant proposals, papers, and presentations or for sharing with colleagues, and they can be printed for documentation in laboratory notebooks. A Chemiluminescent ELISA arrays. The Quansys Biosciences Q-Plex array has distinct capture antibodies bound to each well of a -well plate. The attributes of high resolution and sensitivity enable the ChemiDoc XRS+ system to image the entire plate (panel A) while resolving each array spot (panel B) with the dynamic range (panel C) to provide quantitative data (panel D). B C D The ChemiDoc XRS+ system is flexible and easy to use and supports other detection methods including fluorescence and colorimetry. The versatile optics and large imaging area are useful for many types of samples from large handcast gels to small precast gels and single or multiple blots. The ChemiDoc XRS+ system is the ideal complement to your electrophoresis, purification, and PCR systems, enabling image analysis and documentation of western blots, protein and DNA gels, cell colonies, and other sample types. A B Molecular biology applications. The ChemiDoc XRS+ system can image and analyze a variety of DNA gels and sample sizes. The lighttight enclosure eliminates the need for a darkroom and reduces user exposure to UV rays. Sensitive optics with high resolution and high dynamic range ensure signal detection at safe viewing conditions. The system is sophisticated, easy to use, and provides quick sample analysis results. A, EtBr-stained gel; B, gel stained with SYBR Safe stain. ChemiDoc XRS+ system camera. Emission filter, SYBR Safe stain. XcitaBlue conversion screen.

An alternative to UV illumination to better preserve DNA samples. Left, serial dilution of EtBr stained precision molecular mass ruler (Bio-Rad Laboratories, Inc.

bp band has an initial load of. ng, and the ChemiDoc XRS+ system detects down to 00 pg.

The image of the gel stained with SYBR Safe stain was taken using the XcitaBlue conversion screen and a filter for SYBR Safe stain and GFP.

Documentation of protein gels for lab notebooks or sample analysis, including protein purity and concentration assessment for differential protein expression studies or to monitor gene modulation

Fluorescence, RU,000,000,000,000 0,000,000,000,000 Ethidium Bromide and SYBR Safe DNA Stain Detection SYBR Safe and XcitaBlue screen EtBr and UV radiation Protein gels fluorescence-stained with SYPRO

Resolve closely spaced spots or bands for protein profiling, quantitation, and characterization.,000 0.

A, save and recall image acquisition settings for reproducible imaging conditions; B, use the annotation tools to label images for presentations and publications. Text can be copied between images.

Counting colonies expressing proteins of interest and monitoring protein purification with in-gel GFP fluorescence are performed easily with the analysis and visualization tools available in the

Total protein staining with Coomassie Blue stain and GFP fluorescence can be overlaid using the multichannel viewer, and purity analysis is performed using the band analysis software feature.

6 An alternative to UV illumination to better preserve DNA samples. Left, serial dilution of EtBr stained precision molecular mass ruler (Bio-Rad Laboratories, Inc.) on agarose gel imaged with UV light; right, serially diluted precision molecular mass ruler stained with SYBR Safe stain on agarose gel imaged with the XcitaBlue conversion screen. Lane,. bp band has an initial load of. ng, and the ChemiDoc XRS+ system detects down to 00 pg. There is no loss in sensitivity when a combination of SYBR Safe nucleic acid fluorescent stain and less harmful blue excitation is used instead of UV-excitable EtBr. The image of the gel stained with SYBR Safe stain was taken using the XcitaBlue conversion screen and a filter for SYBR Safe stain and GFP. Less harmful detection methods preserve samples better and more efficiently for downstream uses such as cloning. Protein gels stained with Coomassie Blue stain (left) and silver stain (right). Documentation of protein gels for lab notebooks or sample analysis, including protein purity and concentration assessment for differential protein expression studies or to monitor gene modulation products is supported by the array of analysis tools in the ChemiDoc XRS+ system. Fluorescence, RU,000,000,000,000 0,000,000,000,000 Ethidium Bromide and SYBR Safe DNA Stain Detection SYBR Safe and XcitaBlue screen EtBr and UV radiation Protein gels fluorescence-stained with SYPRO Ruby stain (left) and Flamingo fluorescent gel stain (right). Achieve higher levels of sensitivity with fluorescent stains to differentiate proteins with low expression. Resolve closely spaced spots or bands for protein profiling, quantitation, and characterization., Sample load, ng A B Quantity One software enables molecular weight determination, purity analysis, and lane profile comparison and can be customized by the user. A, save and recall image acquisition settings for reproducible imaging conditions; B, use the annotation tools to label images for presentations and publications. Text can be copied between images. Fluorescence tools. Counting colonies expressing proteins of interest and monitoring protein purification with in-gel GFP fluorescence are performed easily with the analysis and visualization tools available in the ChemiDoc XRS+ system. With the XcitaBlue conversion screen, GFP colonies can be imaged and counted using the colony counting software feature. Total protein staining with Coomassie Blue stain and GFP fluorescence can be overlaid using the multichannel viewer, and purity analysis is performed using the band analysis software feature. Quantity One -D Analysis Software Quantity One software is a powerful and intuitive package for imaging and analyzing -D electrophoretic gels and blots, dot blots, slot blots, and colonies. Quantity One software analyzes a variety of data and automates analysis for fast, high-quality results. User-friendly Interface n Built-in quick guides n Customizable and recallable acquisition settings n Annotation tools n Interactive help guides Analysis Tools n Automatic band and lane detection n Molecular weight determination and purity analysis n Volume analysis for relative differential comparison of samples across different cell lines or against a reference sample n Colony counting to monitor growth of plaques and colonies n Automation manager for archiving and retrieving your most commonly used analysis templates n Viewer for critical -D analysis of closely spaced bands n Multichannel viewer to overlay multiplexed or multifluorescent samples n Lane profile comparison for easy comparison across samples Data Output n Data sharing with unlimited copies of Quantity One Basic software n High DPI TIFF file import, export, and publishing n JPEG file export n Mac and PC compatibility n Networkability Data Security n Tools for compliance with U.S. FDA CFR Part regulations

7 Molecular Imager ChemiDoc XRS+ Systems Overview of ChemiDoc XRS+ System Features High Sensitivity and High Resolution for Improved Quantitation on a Flexible and Easy to Use Platform Feature Benefit Value Motorized zoom lens with numerical feedback, gel alignment templates, and software acquisition presets integrated into an intuitive and easy to use interface Ability to resolve closely spaced bands and detect signals from a wide range of sample intensities Gel alignment templates matched to Sub-Cell trays, Criterion and Ready Gel gels, and precut membranes Lighttight darkroom contains optics engineered with high quantum and photo collection efficiency, high dynamic range, and ultralow dark current Blue illumination integrated into an imaging system with software-controlled illumination User can customize, save, and recall acquisition settings for consistent imaging conditions User can select optimal settings, especially in a photon-starved application such as chemiluminescence, without guesswork Especially useful for western blots with cell lysates and for SDS-PAGE of fractionation samples Enables reproducible positioning and centering of gels on the transilluminator or XcitaBlue conversion screen Ensures signal detection from low-abundance samples and weakly emitting chemiluminescent detection kits; excellent signal-to-noise ratio without saturating highly abundant total protein or control samples Coupled with blue-excitable DNA fluorescent stains such as GelGreen, SYBR Safe, and SYBR Green, image acquisition of DNA electrophoresis samples can be done without exposing samples or users to harmful UV radiation Removes variable imaging conditions so experimental results are minimally affected by the imaging system Reduces learning curve and need for retraining, increases laboratory use of the imaging system, and lessens tendency to revert to film usage Size and quantitate proteins of interest; produce data and publication-quality images Reproducible imaging conditions reduce variability of results between similar samples Enables quantitation of differential expression between cell lines to track and monitor proteins of interest and eliminates the need for a darkroom and the waste and cost involved with film development Preserves samples for downstream use such as molecular cloning and protein production Specifications Application Fluorescence (EtBr, SYBR Green, SYPRO Ruby, Flamingo fluorescent gel stains) Colorimetry (Coomassie Blue, silver stain, blots, prestained markers) Colony plates (GFP, colorimetric) Chemiluminescence Chemifluorescence Fluorescent western blots Dynamic flat fielding*, CV** % Detector CCD resolution (H x V) Pixel density Pixel size (H x V) Resolution Dynamic range Dark current Camera cooling system Camera cooling temperature, x,00 μm, gray levels. x. μm. megapixel orders of magnitude 0.00e-/p/s Peltier 0 o C Quantum efficiency at nm (chemiluminescence) % Quantum efficiency at 00 nm (EtBr) 0% Peak quantum efficiency % Lens Motorized zoom lens Motorized zoom lens numerical feedback Lights Illumination modes Excitation source Software controlled Preparative UV mode for DNA band excision C-mount, f/., mm, f/0. wide angle (optional) on zoom, focus, iris Trans-UV, white, epi-white, trans blue, 0, nm, white light, and blue (XcitaBlue conversion screen) Filters Filter positions Fluorescence: ; chemiluminescence: Emission filiters Darkroom Transillumination area Dimensions (W x D x H) Weight Interface Software Save user customer acquisition settings Acquisition and analysis quick guides included (SYBR Green, EtBr), optional x cm 0 x x cm kg (0. lb) USB (darkroom) and FireWire (camera) -D viewer Multichannel viewer Image stack tool Image background subtraction and filtering methods Image flipping and rotation Volume methods Quantity calibration standards Save analysis settings Colony counting System Operating system compatibility U.S. FDA CFR Part compliance Support Technical support, analysis quick guides different methods methods Band, lane, gel Colonies, plaques, blue/white colonies Windows XP and Windows Vista; Mac OS X, Intel Scientific staff are available to provide unlimited technical support. * U.S. patent,,. ** Coefficient of variation.

8 Ordering Information Catalog # Description Molecular Imager ChemiDoc XRS+ Systems 0- Molecular Imager ChemiDoc XRS+ System, PC 0- Molecular Imager ChemiDoc XRS+ System, Mac Accessories 0- XcitaBlue Conversion Screen and viewing goggles for SYBR Green/SYBR Safe/GFP/Flamingo Fluorescent Gel Stain 0- XcitaBlue Conversion Screen, viewing goggles, and SYBR Safe filter 0- Gel Alignment Templates 0-0 White Light Transilluminator, universal hood 0-00 White Light Conversion Screen 0-0 f/0. mm Wide-Angle Lens 0-0 f/0. mm Wide-Angle Lens 0-0 Filter, 0DF0 mm, for SYBR Safe/SYBR Green/ GFP/SYBR Gold/fluorescein 0-0 Filter, 0DF0 mm, for Cy/rhodamine 0-0 Filter, 0BP0 mm, for SYPRO Ruby/Texas Red 0-0 Filter, 0BP0 mm, for Hoechst/coumarin 0-0 nm UV Lamps, 0- nm UV Lamps, 0-0 Standard 0 nm UV Lamps, 0-0 Mitsubishi PDW Printer 0- Mitsubishi Thermal Printer Paper, rolls 0-0 Sony UPD Printer Paper, rolls Computers Inquire GXP Compliance 0- Quantity One CFR Module 0-0 IQ/OQ, ChemiDoc XRS+ System Analysis Software 0-00 Quantity One -D Analysis Software 0-0 Quantity One -User Network License 0-0 Quantity One -User Network License 0-0 Quantity One -User Network License 0-0 Quantity One -User Network License 0-0 Quantity One -User Network License 0-0 Quantity One 0-User Network License 0-0 Quantity One 0-User Network License 0-0 Quantity One Add User to Network License 0-0 PDQuest Basic -D Analysis Software 0-0 PDQuest Advanced -D Analysis Software 0- PDQuest Advanced -User Network License 0- PDQuest Advanced 0-User Network License 0- PDQuest Advanced 0-User Network License 0- PDQuest Advanced Add User to Network License 0-0 PDQuest Basic to Advanced Software Version Upgrade 0- PDQuest Advanced CFR Module Catalog # Description Stains and Standards 0-00 Immun-Star WesternC Chemiluminescent Kit, includes 0 ml luminol/enhancer, 0 ml peroxide solution -0 Precision Plus Protein WesternC Standards, 0 μl, 0 applications -00 Precision Protein StrepTactin-HRP Conjugate, 00 μl, 0 applications 0-0 Immun-Star Goat Anti-Mouse (GAM)-HRP Detection Kit, 00 ml 0-0 Immun-Star Goat Anti-Rabbit (GAR)-HRP Detection Kit, 00 ml 0-0 Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate, ml 0-0 Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate, ml 0-0 Precision Molecular Mass Ruler, 0 µl, 00 applications -0 Precision Plus Protein Unstained Standards, ml, 00 applications -0 Precision Plus Protein All Blue Standards, 00 μl, 0 applications -0 Precision Plus Protein Dual Color Standards, 00 μl, 0 applications -0 Precision Plus Protein Kaleidoscope Standards, 00 µl, 0 applications -0 Silver Stain Plus Kit -00 Flamingo Fluorescent Gel Stain, 0x solution, 0 ml -0 Ethidium Bromide Solution, 0 mg/ml, 0 ml Blotter Systems -0 Criterion Cell/Plate Blotter System -0 Criterion Cell/Wire Blotter System 0- Trans-Blot SD System and PowerPac HC Power Supply 0- Mini Trans-Blot Cell and PowerPac HC Power Supply -0 Nitrocellulose/Filter Paper Sandwiches, 0. µm,. x. cm, 0 pack -0 Nitrocellulose/Filter Paper Sandwiches, 0. µm,. x. cm, 0 pack -0 Immun-Blot PVDF Membrane, x. cm, 0 sheets -0 Immun-Blot PVDF Membrane, cm x. m, roll -0 Immun-Blot PVDF/Filter Paper Sandwiches, x. cm, 0 pack Coomassie is a trademark of BASF Aktiengesellschaft. Cy is a trademark of GE Healthcare group companies. FireWire, Mac, and Mac OS are trademarks of Apple Inc. GelGreen is a trademark of Biotium, Inc. GelStar is a trademark of FMC Corporation. Hoechst is a trademark of Hoechst GmbH. Mitsubishi is a trademark of Mitsubishi companies. NanoOrange, OliGreen, PicoGreen, Pro-Q, and Qdot, SYBR, SYPRO, and Texas Red are trademarks of Invitrogen Corp. Q-Plex is a trademark of Quansys Biosciences. Sony is a trademark of Sony Corporation. Windows and Windows Vista are trademarks of Microsoft Corporation. Practice of the polymerase chain reaction (PCR) may require a license. Strep-Tactin is covered by German patent application P#.. Bio-Rad Laboratories, Inc. is licensed by Institut für Bioanalytik GmbH to sell these products for research use only. Bio-Rad Laboratories, Inc. Life Science Group Web site USA 00 BIORAD Australia 0 00 Austria 0 0 Belgium 0 Brazil 00 Canada 0 China 00 Czech Republic 0 0 Denmark 0 00 Finland France 0 Germany 0 0 Greece 0 0 Hong Kong 00 Hungary 00 India 000 Israel 0 00 Italy 0 0 Japan Korea 0 Mexico 0 The Netherlands 0 0 New Zealand Norway 0 Poland Portugal 00 Russia 0 Singapore South Africa Spain 0 00 Sweden 0 00 Switzerland 0 Taiwan United Kingdom Bulletin US/EG Rev A Sig 00

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