LIFE SCIENCES NEWS VOL. 4 SEPTEMBER 2017

Transcription

1 Dear customer We are delighted to send you the third edition of our «Life Sciences News» Vol. 4 including some of the latest updates of our Life Sciences product portfolio. Our Marketing and Sales Team will be more than happy to support and assist you for any further information. Your sincerely Chemie Brunschwig AG Team LIFE SCIENCES NEWS VOL. 4 SEPTEMBER 2017 Contents jetcrispr RNP transfection reagent from Polyplus, incl. Promotion page 2 7 tips for optimal DNA Transfection from Polyplus page 3 Pancoll separating solutions from PAN-Biotech, incl. Promotion page 4 Antibiotics from PAN-Biotech, incl. Promotion page 5 Drop-n-Stain Secondary Antibodies from Biotium, incl. Promotion page 6 Antibody purification from ABT, incl. Promotion page 7 D-DiGit Gel Scanner from LI-COR page 8 Horizontal Gel boxes from Axygen, incl. Promotion page 9 Invisorb Fragment CleanUp kit from Stratec Molecular, incl. Promotion page 10 qmaxsen qpcr Mastermixes from Canvax, incl. Promotion page 11 TruePure dntps from Canvax, incl. Promotion page 12

2 jetcrisp RNP transfection reagent for genome editing from Polyplus transfection The leading technology for CRISPR/Cas9 RNP delivery! Specifically designed for Cas9 protein and guide RNA delivery High genome editing efficiency Excellent cell viability and morphology Fast and reliable gene editing Easy to use: reverse and forward protocols Technical protocol of use and free samples (0.1ml size) are available, please ask us. SPECIAL OFFER: 35% discount on 0.75 ml pack size, order it now! jetcrispr RNP transfection reagent ml CHF Also available in 0.1 ml (Cat-No ) and 1.5 ml (Cat-No ) * 1.5 ml of jetcrispr transfection reagent is sufficient to perform ca. 300 transfections in 6-well plates. *Price is net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until

3 7 Tips for optimal DNA Transfection from Polyplus Transfection Follow this guide to ensure that you obtain reproducible and reliable results. DNA transfection is a main tool for current genetics as well as cell and molecular biology studies. Understanding the underlying mechanisms and the different parameters affecting transfection is crucial for optimal, reproducible, and trustable results. Following these seven tips will ensure reliable results. Adapt the transfection conditions to your experiment. Transfection conditions should be optimized according to the cells, the size of the plasmid, the number of plasmids, the expression pattern of the gene of interest, the culture vessel, and the transfection reagent. Reoptimize the conditions for each new cell line. Transfect healthy cells. Passage cells at least twice after thawing to allow recovery before transfection, and use cells at low passage number (< 20 passages). Discard overconfluent cells. Regularly check for mycoplasma contaminations. Seed cells the day before transfection accordingly to the confluency recommended by the transfection reagent provider. Follow the transfection reagent protocol. Some reagents are inhibited by serum or antibiotics, while others may be used in serum- and antibiotic-containing medium, hence reducing the risks of toxicity and the number of steps in the protocol. Check the recommended cell confluency, as well as the recommended DNA amount and reagent volume. Work with high-quality DNA preparation. Check for RNA contamination by agarose gel electrophoresis and ethidium bromide staining. Measure UV absorbance at 280 nm. OD260/280 ratio should reach at least 1.8. Resuspend the plasmid in deionized water or TE buffer at a concentration of ca. 1 µg/µl. Aliquot the plasmid preparation and store at -20 C to avoid freeze/thaw cycles. Check serum quality. Some serum lots may inhibit drastically transfection efficiency, hence resulting in lower silencing efficiency. Check transfection efficiency of different serum lots before purchasing a new batch of serum. Also ensure that the medium used allows for efficient transfection, as some media compound may decrease transfection efficiency. Minimize cytotoxicity by using low DNA amount and low transfection reagent volume. Check that the target gene does not affect cell viability. Analyze transfection at an earlier time point (24 h after transfection instead of 48 h for instance). Use appropriate controls. Use a reporter gene to set up and optimize transfection conditions, as those may vary depending on the cells to transfect. Various reporter systems are commercially available: Renilla Luciferase and GFP (Green Fluorescent Protein) are the most commonly used. Polyplus-transfection created a database where scientists can find optimal conditions to transfect their cells: Search in the Polyplus-Transfection Database ( The reagent of choice: jetprime (Cat-No , & ). Free samples are available. Enjoy the product video on our website (Suppliers/ Polyplus transfection) 3

4 Pancoll, Separating Solutions from PAN-Biotech Description In many cases, the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Physical separation methods are most commonly used with physical differences such as size and weight of the particles to be separated. For this purpose so-called separating solutions are used. Pancoll separating solutions contain a polysaccharide with a molecular weight of 400'000 daltons; this hydrophilic polymer allows for production of aqueous solutions for cell separation with a density of up to 1.2g/ml. PAN-Biotech offers a variety of ready-to-use products with a density of g/ml up to g/ml for a very wide range of cell separation applications. The ready-to-use solutions are available in 500ml bottles as well as in pre-filled ready-to-use tubes with a separating membrane. Product catalog or product flyers are available, please ask. SPECIAL OFFER: 20% discount on all products listed below! Ready-to-use solutions Pancoll human, density g/ml P ml CHF Pancoll mouse, density g/ml P ml CHF Pancoll rat, density g/ml P ml CHF Pancoll monocytes, density g/ml P ml CHF Pancoll platelets, density g/ml P ml CHF Pre-filled ready-to-use tubes with separating membrane Pancoll human, density g/ml P P x 50ml 50 x 10ml CHF CHF *Price is net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until

5 Antibiotics from PAN-Biotech PAN-Biotech produces and supplies a wide range of high quality antibiotics and antifungal drugs for the use in cell culture. All PAN-Biotech reagents are tested under highest quality standards to provide you with high quality products that are consistent from batch to batch. All liquid reagents are sterilized add filtered at 0.2um into gamma irradiated PET bottles. Complete Antibiotics & Antifungal Drugs list is available, please ask us. SPECIAL OFFER: benefit from attractive prices on all products listed below! Benefits of PAN-Biotech Antibiotics: easy to use reliable effective in their application Amphotericin B, 250 ug/ml P ml CHF Gentamycin sulphate, 50 mg/ml P ml CHF Gentamicin sulfate, 10 mg/ml P ml CHF Geneticine (Paneticin G 418) 50 mg/ml P ml CHF Hygromycin B (50 mg/ml) P ml CHF Hygromycin B, 50 mg/ml P ml CHF Kanamycin sulfate, 5 mg/ml P ml CHF Neomycin sulfate, 10 mg/ml P ml CHF Neomycin sulfate, Powder P P 10 g CHF Paneticin G 418, Powder P P 5 g CHF Paneticin G 418, Powder P P 10 g CHF Penicillin-Streptomycin, 10'000U/ml Penicillin, 10 mg/ml Streptomycin P ml CHF Zeocin (100 mg/ml) P ml CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until Products are also listed on our GoodDeal Promotion

6 Drop-n-Stain Secondary Antibodies: fast & easy, no pipetting from Biotium Drop-n-Stain Secondary Antibodies are provided in a convenient dropper bottle for easy dispensing without pipetting. Drop-n-Stain secondaries feature affinity-purified, highly cross-adsorbed secondary antibodies conjugated to Biotium s CF dyes. CF dyes are superior to Alexa Fluor, DyLight, and Cy dyes for antibody labeling by having combined advantages in brightness, photostability, and specificity. Drop-n-Stain secondaries come in four reactivities: Donkey anti-mouse IgG (H+L), highly cross-adsorbed Donkey anti-rabbit IgG (H+L), highly cross-adsorbed Goat anti-mouse IgG (H+L), highly cross-adsorbed Goat anti-rabbit IgG (H+L), highly cross-adsorbed Drop-n-Stain secondaries are offered in four fluorescent dye conjugates: Product Information Sheet, Product Selection Guide and Quick Dye Reference Table are available please ask. SPECIAL OFFER: 20% discount on all products listed below! CF 488A is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. CF 488A has brightness and photostability comparable to Alexa Fluor 488. CF 543 is an orange fluorescent dye with an excitation spectrum optimally matching the 543 laser line. Antibody conjugates of the dye are much brighter than those of Alexa Fluor 546. CF 594 is spectrally similar to Alexa Fluor 594 and Texas Red dye but yields much brighter protein conjugates due to its high quantum yield and exceptional water solubility. CF 594 also has excellent photostability ideal for demanding applications, such as confocal microscopy and single molecule imaging. CF 640R is a novel rhodamine-based dye spectrally very similar to Cy 5 and Alexa Fluor 647. CF 640R is much brighter than Cy 5 and at least as bright as Alexa Fluor 647 when excited at 633 or 635 nm. However, the most important advantage of CF 640R is its exceptional photostability. CF 640R is far more photostable than the cyanine-based dyes Cy 5, Alexa Fluor 647 and Atto 647N. Product Description + Cat-No Size Special Price* Drop-n-Stain Secondary Antibodies Cat-No , 20951, 20952, 20953, 20954, 20955, 20956, 20957, 20962, 20963, 20964, 20965, 20966, 20967, and ml CHF *Price is net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until

7 Antibody purification from ABT Excellent performance at a great price High binding: 25 mg IgG / ml resin High stability binding capacity rprotein A No leakage and is reusable BEAD GEO METRY & SIZE CROSSLINKED Spherical, Standard: ~ μm Yes AGAROSE % 4% COUPLING METHOD STATIC BINDING CAPACITY ANTIMICROBIAL AGENT Covalent binding by reductive amination ~25 mg human IgG / ml resin 20% Ethanol STORAGE TEMPERATURE 2-8 C High binding: 20 mg human IgG / ml resin High stability binding r Protein G Resin is reusable with no significant loss of binding capacity BEAD GEO METRY & SIZE CROSSLINKED AGAROSE % 4% COUPLING METHOD STATIC BINDING CAPACITY ANTIMICROBIAL AGENT Spherical, Standard: ~ μm Highly crosslinked Coupling binding by reductive amination ~20 mg human IgG / ml resin 20% Ethanol STORAGE TEMPERATURE 2-8 C Product catalog and product specifictions are available. SPECIAL OFFER: 25% discount on products listed below! Protein A Agarose Resin PA ml CHF Protein Agarose Resin 4 Rapid Run 4RRPG-5 5 ml CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until Products are also listed on our GoodDeal Promotion

8 D-DiGit a fully integrated DNA Gel Scanner that is simple and affordable - from LI-COR Stained with GelRed D-DiGit 12X exposure Stained with SYBR Safe D-DiGit 4X exposure Make your nucleic acid gel imaging safe and worry-free The D-DiGit Gel Scanner is an all-inclusive system for detection and gel extraction of DNA and RNA stained with fluorescent safe stains. Now you can visualize sample migration and conduct gel excision without contamination or the need to transfer gels between viewing and cutting surfaces. This economical, benchtop gel scanner lets you: Safeguard your samples from strand nicking caused by UV irradiation Illumination provided by blue LED light source Compatible with most nucleic acid safe stains including SYBR Safe DNA Gel Stain, GelGreen Nucleic Acid Stain, and GelRed Nucleic Acid Stain Strengthen your data with excellent detection sensitivity Detects as little as 0.04 ng nucleic acid per band Detection sensitivity is comparable to UV-based detection using ethidium bromide Simplify your workflow on an easy-to-use imaging platform Large scan surface (17.8 cm x 12.7 cm/7 x 5 ) for imaging most gel sizes, and band extraction Acquisition software that produces data images compatible with Image StudioTM Software for analysis, quantitation, and archiving. Product Manual and Quick Start Guide are available. Please contact us if you would like to receive a demonstration. 8

9 Horizontal Gel boxes from Axygen Axygen horizontal gel boxes are high quality, full featured units designed for exceptional durability and efficiency. The gel boxes feature leak-free external gel casting, which allows you to cast one gel outside of the box while another gel is running inside. The units are available in four different sizes, and each unit comes with two sizes of gel trays. In addition, they have easy to replace electrodes that are recessed to reduce potential damage. These gel boxes are an excellent value that will provide years of reliable service. Product Selection Guide and Manual are available, please ask us. SPECIAL OFFER: 26% discount on all products listed below! Features: Flexibility each unit comes with 2 sizes of gel trays to optimize applications. External gel casting to gel box allows for running gels while casting the next one. Ease of use the Axygen contrast enhancer makes loading gels easier. Rugged injection molding is supported by a 3-year warranty. Larger units come with recirculation ports allowing for buffer exchange. Four sizes of horizontal tanks. Each comes with 2 sizes of gel trays which allow for external gel casting. Three larger sizes come with recirculation ports for use as needed. Axygen horizontal gel box, 7 cm CNEHGB-7 1 each CHF Axygen horizontal gel box, 10 cm CNEHGB-10 1 each CHF Axygen horizontal gel box, 15 cm CNEHGB-15 1 each CHF Axygen horizontal gel box, 20 cm CNEHGB-20 1 each CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until

10 Invisorb Fragment CleanUp kit from Stratec Molecular Gel and PCR Cleanup system (2 kits in 1) The Invisorb Fragment Cleanup kit from Stratec Molecular provides a convenient tool for fast and efficient DNA fragment purifications from agarose gels or liquid solutions (PCR reactions, cdna synthesis, restriction digestion mixtures, etc ) Main benefits: Optimized buffer solutions provided to meet the best results. PCR cleanup ready in 7 minutes - only 2 steps: binding and elution (MSB technology) High quality (DNA suitable for cloning, PCR, digestion, sequencing, hybridization, labeling..) High recovery (up to 95%) and reproducibility Elution volume as little as 10 μl, also suitable for sample concentration Purification of DNA fragments from 80bp up to 30kbp Dye removal from Cycle-Sequencing reactions For more information, please ask us. SPECIAL OFFER: benefit from attractive prices on these 2 products below. Starting Material Average yield Preparation time up to 100 μl PCR-, cycle sequencingcdna syntesis-, ligation- and restriction digestion reaction mixture Product characteristics up to 95 %, depending on fragment length and kind of agarose gel 7 min for PCR cleanup 20 min for gel extraction up to 300 mg gel slices from TAE or TBE agarose gels Invisorb Fragment CleanUp 50 purifications kit CHF Invisorb Fragment CleanUp 250 purifications kit CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until

11 qmaxsen qpcr Mastermixes from Canvax Canvax qmaxsen qpcr MasterMixes offers next Benefits & Advantages: qmaxsen qpcr MasterMixes offers an exceptional Sensitivity and Performance for the two most used qpcr methods: intercalating Green Dyes based or Probe-based assays. Also shows a great versatility due its compability with the most used real-time PCR instruments and includes the option of ROX as the internal passive reference dye which the reporter-dye signal can be normalized during data analysis. Exceptional Sensitivity High Specificity Versatile: compatible with most used real-time PCR instruments Convenient: includes internal passive reference dye Great signal-to-noise ratio Safe: minimize contamination risk due its optimized protocol that reduces pipetting steps. Accurate Quantification Price-Advantage Longer Shelf life: 24 months from the date of purchase due a novel manufacturing process Results or Refunds: product covered by Canvax Risk-free Warranty qmaxsen Green qpcr MasterMix Supplied as stabilized 2x reaction mixture, is a convenient and easy, ready-to-use premix to perform qpcr using an analogue Green fluorescent dye to SYBR Green. The optimized MasterMix includes all the components required for running PCR reactions, except PCR Primers and template. qmaxsen Green qpcr MasterMix (w/o ROX ) E x 1.25ml (250 rxn) CHF qmaxsen Green qpcr MasterMix (w/o ROX ) E x 1.25ml (500 rxn) CHF qmaxsen Green qpcr MasterMix (Low ROX ) E x 1.25ml (250 rxn) CHF qmaxsen Green qpcr MasterMix (Low ROX ) E x 1.25ml (500 rxn) CHF qmaxsen Green qpcr MasterMix (High ROX ) E x 1.25ml (250 rxn) CHF qmaxsen Green qpcr MasterMix (High ROX ) E x 1.25ml (500 rxn) CHF qmaxsen Probe qpcr Mastermix, offers exceptional Sensitivity, Performance & signal-to-noise ratio in real-time PCR protocols. It has been formulated specifically for TaqMan Probe-based real-time PCR analysis of DNA samples. The optimized MasterMix includes all the components required for real-time PCR, except sample DNA and Primers, to ensure fast and easy preparation with a minimum of pipetting steps. qmaxsen Probe qpcr Mastermix (ROX ) E x 1.25ml (250 rxn) CHF qmaxsen Probe qpcr Mastermix (ROX ) E x 1.25ml (500 rxn) CHF qmaxsen Probe qpcr Mastermix (Low ROX ) E x 1.25ml (250 rxn) CHF qmaxsen Probe qpcr Mastermix (Low ROX ) E x 1.25ml (500 rxn) CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until Larger sizes (up to 50ml) are available, please contact us. 11

12 TruePure dntps from Canvax Highest Purity (>99%) & Stability for High-End TruePure dntps The need of ultrapure deoxynucleotide triphosphates (dntps) in long PCRs, RT-PCRs and proofreading amplifications is critical. Canvax offers TruePure dntps, manufactured by a Certified Quality Management System where each dntp lot is assayed by the most stringent criteria regarding Purity and Functionality. Canvax TruePure dntps has the following Advantages and Features: Complete product catalogue is available, please ask us. Highest Purity: greater than 99% confirmed by HPLC Highly stable Proven Performance for PCR and most common Molecular Biology applications Time-saving: ready-to-use format that eliminates experiment preparation time High Quality: free of DNAse, Protease, Phosphatase, Nuclease, Human or E. coli DNA contamination Longer Shelf life: 24 months from the date of purchase due to a novel Treatment Results or Refunds: product covered by Canvax Risk-free Quality 100% Guaranteed SPECIAL OFFER: benefit from more than 40% discount on products listed below. TruePure dntp Mix, Concentration: 40mM N0033-S 1ml, 10 nm each nt CHF TruePure dntp Mix, Concentration: 40mM N0033 5x1ml, 10 nm each nt, CHF TruePure dntp Set, Gel Pack N0098-S 4 x 0.2 ml of 100 mm CHF TruePure dntp Set, Gel Pack N x 0.4 ml of 100 mm CHF * Prices are net, without any cumulative discount, ex works and excl. V.A.T. Offer valid until Chemie Brunschwig AG Auf dem Wolf 10 CH-4052 Basel T F info@brunschwig-ch.com 12