chemagic STAR New methods for total RNA extraction Standard Solutions Workstation for nucleic acid extraction

Transcription

1 chemagic STAR Standard Solutions Workstation for nucleic acid extraction New methods for total RNA extraction

2 Highest quality chemagen s magnetic bead technology yields in ultrapure nucleic acids Due to chemagen s proprietary Polyvinyl Alcohol particles (M-PVA Magnetic Beads) this extraction technology yields in ultrapure nucleic acids. The hydrophilic surface of these beads results in high affinity to nucleic acids. Once bound to the M-PVA Magnetic Beads, the nucleic acids are transferred with a magnetisable rod to different wash buffers and finally to the elution buffer. This method ensures both: highest purity and reliability. The rod itself is protected by a single disposable sleeve. During the whole isolation process mixing, binding and elution is achieved through the patented rotation of the rods when the magnet is switched off. This technique does not lead to DNA shearing and supports an efficient binding of nucleic acids to the beads. No cross contaminations occur during the isolation process. Ready to Start DNA/RNA extraction protocols for different volumes 34 different chemagic Kits tested Extract from sample volumes from 20µl to 500µl with the chemagic 96 Rod Head Extract from sample volumes from 500µl to 4ml with the chemagic 24 Rod Head Extract from sample volumes of 1ml to 10ml with the chemagic 12 Rod Head chemagen s extraction technology

3 Unique flexibility Using chemagen s proven nucleic acid extraction technology Automating nucleic acid purification is often a trade-off between quality and throughput: good stand-alone purification systems lack liquid handling support for walk-away operation. In addition, liquid handling workstations often require tedious adaptation of manual protocols. The new chemagic STAR combines chemagen s leading NA purification system with the best automated liquid handling technology to offer you stress-free operation in one benchtop system. The chemagic STAR can process samples from 20µl up to 10ml. The system is available with three extraction heads - the low volume chemagic 96 Rod Head for extractions up to 500µl per sample, the chemagic 24 Rod Head for 500µl - 4ml samples and the high volume chemagic 12 Rod Head for extractions in the range from 1 to 10ml. Wide range of sample material like blood, buffy coat, saliva, cells, tissue, buccal swabs (DNA), plasma, other human body fluids (viral/pathogenic DNA/RNA) and PAXGene blood, tissue and cells (totalrna) can be processed with the chemagic STAR. The extracted nucleic acids are ready to be used in the fields of HLA-typing, Human Genetics, Pathogen Diagnostics and Virus screening. Safe contamination free primary sample pipetting with filter tips Additional modules like 2D barcode scanning or LIMS integration available Customized applications possible like PCR setup, concentration measurement or DNA normalization Optional: integrated lot tracking of the used chemagic STAR Kit Technology Traceability/Process safety: Barcode reading from primary sample tubes, storage racks and tip carriers, verifies correct labware positions and loads carriers automatically to the system for greater method security. Sample/Data tracking during the extraction process MAD: by monitoring the air-based pipetting action, the instrument detects clots or empty wells during the aspiration step in real time. Integrated design: Single enclosure ensures minimal transport distances One cockpit for your complete process Minimal space required Accessories: Disposables used in the extraction process (sleeves, elution tubes, 24- or 96 deep well plates) are included in the chemagic STAR Kit Disposable tip consumption reduced to a minimum of 1.4 tips/sample (96 format)

4 ENHANCE WALK-AWAY TIME AND THROUGHPUT 2.5-4h for 2 sample sets (2x12, 2x24, 2x96) depending on volume, quality and number of samples, used protocol and configuration Up to 72 high, 96 medium or 576 low volume samples per working day High throughput

5 Specifications System: Complete nucleic acid extraction from biological samples and creation of report files in a non IVD Intended use environment. Further applications on the STAR liquid handling system are available on request. System dimensions Width: 2360mm, height: 945.5mm, depth: 1006mm Weight 215kg PC (included) Intel Core 2 Duo E8400 (3GHz,1333MHz,6MB), 2048 MB RAM, 2x250GB harddisk RAID 1, Microsoft Windows XP Professional, 6 USB and 2 RS232 circuit points; or better Communication Pipetting Unit: USB / Data transfer: Peer-to-Peer connection: Extractor: RS232 Operating data Voltage Frequency Operating environment Altitude 115V~ / 230V~ 50Hz / 60Hz C, relative humidity 30 to 85% with no condensation < 2000m Pipetting unit: chemagen extraction heads: Work area dimensions Width: 675mm, height: 195mm, depth: 465mm 20μl - 500μl: chemagic 96 Rod Head Pipetting arm Modular arm, 4 x 1000μl pipetting channels, 4 x 5000μl pipetting channels 500μl - 4ml: chemagic 24 Rod Head Positional accuracy X-Y-Z positional accuracy of 0.1mm 1ml - 10ml: chemagic 12 Rod Head Tip Sizes Low volume: 10μl and 50µl, standard volume: 300μl, high volume: 1000μl and 5000µl tips (detailed pipetting specifications can be found in STAR Line brochure Methods: Low volume 96 samples, 20μl - 500μl Medium volume 24 samples, 500μl - 4ml High volume 12 samples, 1-10ml Genomic DNA μl blood (no. 1757) μl blood (no. 1751) 20-50μl blood (no. 1759) μl buffy coat (no. 1790) from 1ml blood max. 2-4ml blood 1-2ml blood 0.5-1ml blood 0.5-1ml buffy coat from 4ml blood max. (no.1756) (no.1767) (no.1793) (no.1791) 7-10ml blood (no.1710) 5-7ml blood (no.1707) 3-5ml blood (no.1705) 1-3ml blood (no.1703) 1-2ml buffy coat (no.1792) from 10ml blood max μl Oragene -saliva (no. 1770) 1-2 x 10 6 cells (no. 1768) Buccal swab (no. 1758) 10mg tissue (no. 1760) 2-4ml Oragene -saliva (no. 1755) 5-12 x 10 6 cells (no. 1769) 2-4ml Oragene -saliva (no. 1794) 5 x 10 7 cells (no. 1750) Viral NA and pathogenic NA 300μl plasma (no. 1774) 200μl plasma (no. 1773) 100μl plasma (no. 1772) 2.4ml plasma (no. 1776) 1.2ml plasma (no. 1775) 9.6ml plasma (no. 1780) 4.8ml plasma (no. 1779) 2.4ml plsama (no. 1778) 1.2ml plasma (no. 1777) Total RNA 2.5 ml PAXgene TM (no. 1797) 2.5 ml PAXgene TM (no. 1766) blood blood mg tissue (no. 1796) mg tissue (no. 1795) Disclaimer: the chemagic STAR system is classified as a product for general laboratory use and not as an IVD device defined by EU directive 98/79/EC ( in vitro diagnostics ). The system components are conform with EU directives 2004/108/EC ( EMC ), 2006/95/EC ( low voltage ) and Canada&USA directive CAN/CSA-C22.2 (Safety Requirements for Electrical Equipment).

6 2014 Hamilton Bonaduz AG. All rights reserved. All trademarks are owned and/or registered by Hamilton Bonaduz AG in Switzerland and/or other countries. Lit. No. B /2014 Printed in Germany. Web: USA: United States Tel: United Kingdom & Ireland Tel: +44 (0) Brazil Tel: +55 (11) China Tel: France Tel: +33 (01) Italy Tel: To find a subsidiary or distributor in your area, please visit hamiltonrobotics.com/contacts. Denmark, Norway, Sweden, Finland Tel: +46 (0) Germany, Switzerland, Austria, Benelux Tel: +49 (0)