from different sources

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1 Thermo Scientific GeneJET Nucleic Acid Purification Kits high yields of pure & RNA from different sources High Yields Exceptional Purity Incredible Value

2 Reliable and RNA purification The Thermo Scientific GeneJET product line contains essential nucleic acid purification tools to support and facilitate your experimental workflow. With more than years of experience in developing and manufacturing molecular biology products, we see a growing demand for simple and reliable tools for and RNA sample preparation. The GeneJET and RNA purification kits address the need for high-purity nucleic acids ready to use in downstream applications and offer detailed protocols for routine and difficult sample types. Thermo Scientific GeneJET nucleic acid isolation products Trusted silica membrane technology in the form of a convenient spin column for purification of and RNA Isolate nucleic acids with superior purity and yields compared to other kits on the market Purified nucleic acids are ready to use in common downstream applications GeneJET kits have been extensively tested on a wide variety of sample types and include specialized protocols Nucleic Acid Purification Plasmid Genomic Total RNA Fragments Cleanup and Concentration Viral and RNA Maxiprep Plant Plant From PCR RNA From Serum & Plasma Midiprep Blood Blood From Gels Miniprep from Cells & Tissues RNA from Cells & Tissues For further information visit thermoscientific.com/genejet

3 Rapid isolation of high-quality plasmid GeneJET Plasmid Purification Kits GeneJET Plasmid Miniprep Kit Small-scale isolation of up to μg of high-quality plasmid in minutes GeneJET Plasmid Midiprep Kit Medium-scale isolation of up to μg of high-quality plasmid in 6 minutes GeneJET Plasmid Maxiprep Kit Large-scale isolation of up to 7 μg of high-quality plasmid in 6 minutes GeneJET Plasmid Miniprep Kit GeneJET Plasmid Midiprep Kit GeneJET Plasmid Maxiprep Kit K K K8 K8 K9 K9 Silica-based membrane technology save time and improve yields compared to gravity flow columns Isolated plasmid can be used directly in Thermo Scientific FastDigest or conventional restriction digestion, sequencing, PCR, in vitro transcription and transfection of robust cell lines Specifications for GeneJET Plasmid Purification Kits Plasmid Purification Kits Mini Midi Maxi Bacterial culture volume ml ml ml Yield µg µg 7 µg concentration ng/µl ng/µl ng/µl Purity A 6 /A Column binding capacity µg µg µg Procedural time min 6 min 6 min Format and protocol types Spin columns, capped, preassembled with collection tubes. Centrifugation or vacuum protocol. High yields of high concentration plasmid Consistent and reproducible miniprep yields, µg Thermo Scientific vendor A vendor B vendor C st elution nd elution vendor D Yields of high-copy plasmid obtained using GeneJET plasmid midiprep kit and other kits for midscale plasmid purification at maximal recommended bacterial cell culture volumes., ng/µg Thermo Scientific vendor A vendor B vendor C st elution nd elution vendor D Concentrations of eluted obtained after the purification of high-copy plasmid using different kits for midscale plasmid purification at maximal recommended bacterial cell culture volumes. M 6 Relaxed Supercoiled A pbluescript-based high copy number plasmid was purified from E.coli culture with 6 different GeneJET Spin Columns. M - Thermo Scientific FastRuler High Range Ladder -6 - plasmid isolated with 6 different GeneJET Spin Columns.

4 Efficient genomic purification Spin-columns based on silica membrane technology Isolated can be used directly in PCR, qpcr, and other common enzymatic reactions GeneJET Genomic Purification Kits GeneJET Genomic Purification Kit Purify RNA-free g from a wide variety of tissues or cells in only minutes GeneJET Whole Blood Genomic Purification Mini Kit Rapidly purify RNA-free g from whole blood and body fluids with no separate cell lysis step GeneJET Plant Genomic Purification Mini Kit Purify without clogging-prone lysate filtration step Material-specific protocols provided Recover g greater than kb in size, including nuclear, mitochondrial and chloroplast GeneJET Genomic Purification Kit GeneJET Whole Blood Genomic Purification Mini Kit GeneJET Plant Genomic Purification Mini Kit K7 K7 K78 K78 K79 K79 Specifications for GeneJET Genomic Purification Kits Genomic Purification Kits Cells & tissues Whole Blood Plant Amount of sample material Varies µl mg Yield Varies Up to µg Up to µg Purity A 6 /A Column binding capacity µg µg µg Procedural time min min min Spin columns, capped, preassembled with collection Format and protocol tubes and individually packed. Centrifugation or vacuum protocol. Isolate genomic from a variety of samples: Mammalian cells Mammalian tissues: Muscle Heart Liver Whole blood Fresh or frozen Anticoagulated blood Buffy coat Bone marrow Body fluids (urine) Plants Multiple plant species Various plant tissues (leaves, roots, seed, needles) Bacteria Yeasts Filamentous fungi High-integrity genomic purified from various sources High yields of genomic purified from various sources M M Genomic, µg rat liver mg rat spleen mg mouse heart mg HeLa x 8 cells E. coli x 9 cells Thermo Scientific vendor A vendor B vendor C mammalian blood µl Yields of genomic purified using the GeneJET Genomic Purification Kit and kits from other vendors. Genomic from different plant material samples purified using the GeneJET Plant Genomic Purification Mini Kit. - Arabidopsis, - Tobacco leaves, - Tomato leaves, - Spinach leaves, - Wheat leaves, 6- Grape leaves, 7- Onion leaves, 8- Rice leaves, 9- Corn seeds, - Sunflower stalk, - Corn roots, - Beef roots, - Cucumber fruit, - Sugar cane.

5 Isolate high-purity total RNA from routine and challenging sample types GeneJET Total RNA Purification Kits GeneJET RNA Purification Kit Purify total RNA from a wide variety of sources GeneJET Whole Blood RNA Purification Mini Kit Purify RNA from whole blood and body fluids without DNase treatment GeneJET Plant RNA Purification Mini Kit Purify RNA from various plants and plant tissues GeneJET RNA Purification Kit K7 K7 GeneJET Whole Blood RNA Purification Mini Kit K76 GeneJET Plant RNA Purification Mini Kit K8 K8 Related Thermo Scientific RapidOut Removal Kit K98 Specifications for GeneJET RNA Purification Kits RNA Purification Kits Cells & tissues Whole Blood Plants Amount of sample material Varies µl mg Yield Up to 7 µg Up to µg Up to 7 µg Purity A 6 /A Column binding capacity > µg > µg > µg Procedural time min min min Format and protocol types Spin columns, capped, preassembled with collection tubes and individually packed. Centrifugation protocol. Total RNA purified from various sources Isolate RNA from a variety of samples: Mammalian cells Mammalian tissues: Muscle Heart Liver Whole blood Anticoagulated blood Buffy coat Bone marrow Plants Multiple plant species Various plant tissues (leaves, roots, seed, needles) Pathogens (virus) Bacteria Yeasts Filamentous fungi Spin-columns based on silica membrane technology Specialized and detailed protocols for different sample types M Total RNA purified from mg of tomato leaves using the GeneJET Plant RNA Purification Kit and kits from other vendors. M- Thermo Scientific RiboRuler High Range RNA Ladder. - Thermo Scientific, - other vendors. M 6 Total RNA purified from various sources using the GeneJET RNA Purification Kit. M- RiboRuler High Range RNA Ladder (#SM8) - mouse liver, - mouse spleen, - HeLa cells, - Cos-7 cells, - Saccharomyces cerevisiae, 6- Escherichia coli DHα. Purified RNA can be used in a wide range of downstream applications

6 Effective fragment recovery from reaction mixtures or agarose gels GeneJET PCR Purification and Gel Extraction Kits GeneJET PCR Purification Kit recovery up to % ( bp - kb) Effective removal of primers, unincorporated nucleotides, enzymes and salts from PCR and other enzymatic reaction mixtures GeneJET Gel Extraction Kit recovery up to 9% ( bp - kb) Purify fragments from standard or low-melt agarose gels run with TAE or TBE buffer. GeneJET PCR Purification Kit GeneJET Gel Extraction Kit K7 K7 K69 K69 Purified can be used in downstream applications such as sequencing, restriction digestion, ligation, cloning, and in vitro transcription Spin-columns based on silica membrane technology PCR Purification and Gel Extraction Kits PCR Purification and Gel Extraction Kits Kit types PCR Purification Gel Extraction Amount of sample material.8 ml g agarose gel Recovery rate 9-% 7-9% fragment range bp- kb bp- kb Purity A 6 /A Column binding capacity µg µg Procedural time min min Format and protocol types Spin columns, capped, preassembled with collection tubes. Centrifugation or vacuum protocol. Superior extraction of large and small fragments from PCR mixtures Superior extraction of large and small fragments from agarose gels Recovery rate, % 8 6 Thermo Scientific vendor A vendor B Recovery rate, % 8 6 Thermo Scientific vendor A vendor B bp bp kb Size. kb kb bp bp kb Size. kb kb 6

7 Outstanding recovery of pure and concentrated RNA and GeneJET & RNA Cleanup and Concentration Micro Kits GeneJET Cleanup and Gel Extraction Micro Kit 9-% recovery in the -, bp fragment range Efficient removal of reaction components including enzymes, proteins, primers, primer dimers, dntps and salts Flexible does not require precise gel slice weighing Convenient three-in-one format gel extraction, concentration and removal of reaction components GeneJET RNA Cleanup and Concentration Micro Kit 8-% recovery of a high-integrity RNA Exceptional purity efficiently removes enzyme inhibitors as well as DNase I and other enzymes Fast and simple procedure takes less than five minutes Convenient two-in-one format RNA concentration and removal of reaction components GeneJET Cleanup and Gel Extraction Micro Kit GeneJET RNA Cleanup and Concentraction Micro Kit K8 K8 K8 K8 Advanced Column Design Easy and fast to load The proprietary funnel-shaped column design ensures every sample droplet is directed to the filter membrane. This allows convenient and fast sample loading without risk of filter damage or sample loss. Maximized yields Elution volumes, as low as 6 µl, allow for purification of highly concentrated ready-to-use and RNA. Risk-free results Minimized sample retention prevents wash buffer residue carryover contamination. Specification for GeneJET & RNA Cleanup and Concentration Micro Kits & RNA Cleanup and Concentration Micro Kits RNA Recovery rate 9 - % 8 - % Elution volume 6 - µl 6 - µl Column binding capacity µg µg Procedural time min min Unmatched efficiency in primer removal from PCR mixtures Concentrate dilute pre-purified RNA sample without damage to RNA integrity Thermo Scientific Vendor Vendor Vendor Vendor Thermo Scientific Vendor Vendor Vendor PCR product M C M Primerdimers The GeneJET Gel Extraction and Cleanup Micro Kit is ideal for cleanup of PCR reaction mixtures. The kit combines efficient removal of primer dimers and nucleotides without loss of the initial PCR product. A 9 bp PCR product was purified using the recommended primer-dimer removal protocols from different vendor kits. M Thermo Scientific O'GeneRuler bp Ladder (#SM) C Unpurified PCR product, - Thermo Scientific, - Vendor, -6 Vendor. M C M Pre-purified low concentration total RNA from human blood samples was concentrated using recommended protocols from different vendor kits. Purified RNA was analyzed on a % agarose gel. M RiboRuler High Range RNA Ladder (#SM8), C RNA sample before concentration. - Thermo Scientific, - Vendor, -6 Vendor, 7-8 Vendor, 9- Vendor.

8 Fast and efficient viral nucleic acid purification Viral and RNA Purification Kits GeneJET Viral and RNA Purification Kit Linear yields from a wide range of viral titers Suitable for serum, plasma and cell-free liquids Tested with a variety of and RNA viruses (HIV, HBV, HCV, HPV, CMV, EBV) Compatible with sensitive downstream assays such as (RT)- PCR, (RT)-qPCR Viral /RNA Purification Kit (IVD) Efficient purification of viral and RNA from a wide range of virus concentrations Compatible with fresh or frozen serum and plasma perserved with EDTA or citrate Minimized risk of cross contamination CE-marked for compliance with EU Directive 98/79/ECr. GeneJET Viral and RNA Purification Kit K8 IVD Viral /RNA Purification Kit* for in vitro diagnostic use DK *Sales limited within EU. Contact your local provider for product availability in your country. Linear performance across a wide range of viral nucleic acid concentrations Highly efficient nucleic acid purification from various viruses Log copies/ml HBV HCV CMV EBV Thermo Scientific Vendor A Vendor B Vendor C HBV-, HCV-, CMV-, and EBVinfected human plasma or blood samples were purified using the GeneJET Viral and RNA Purification kit and kits from other suppliers. Isolated viral nucleic acids were quantified using a real-time PCR detection assay. Detection of viral RNA A. and B. purified with the GeneJET Viral and RNA Purification Kit in a realtime PCR assay. Samples of various HBV and HCV titers were produced by diluting infected plasma of known viral titer with non-infected plasma. A. Detection of RNA from various HCV sample titers. B. Detection of from various HBV sample titers R² =.998 R² = A Input HCV, log IU/ml Input HBV, log IU/ml Input HCV, log IU/ml B Input HBV, log IU/ml Result HCV, log log IU/ml Fluorescence (norm) A Cycle number Result HBV, log IU/ml Fluorescence (norm) B Result HBV, log IU/ml Cycle number For further information visit thermoscientific.com/ genejet Thermo Fisher Scientific Inc. All rights reserved. pbluescript is a trademark of Agilient Technologies, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. MB9CCG Austria: + () 8 88 Belgique/België: + () Denmark: España: Finland: +8 () France: + () Germany: +9 () 8 8 Ireland: + () 88 8 Italia: Netherlands: + () 87 7 Norway: +7 () Portugal: + / Suisse/Switzerland: + () 6 68 Sweden: +6 () UK: + ()9 66