ICAR MTL WG - Working Group on Milk Testing Laboratories ANALYTICAL METHODS FOR MILK RECORDING ANALYSIS
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1 ANALYTICAL METHODS FOR MILK RECORDING ANALYSIS This document presents a summary of methods and instruments available for milk recording purposes. It is a part of the annexes of ICAR Guidelines for DHI analysis but for reason of convenience, it is aimed at presenting it separately so as to enable regular updates with the evolution of analytical method standardisation. From the last version of 2001, the parts referring to reference methods and other chemical methods have been updated with the material of the international ISO IDF standardisation whereas the information about the routine methods at first established from the replies to enquiries in ICAR countries has been complemented with new methods validated by national bodies of ICAR members and results published. Methods/instruments not produced or used any longer are given for information only and therefore printed in Italic characters. Disclaimer : Part IV of the following list has only an informative objective by ICAR and in no case can constitute a kind of international approval by ICAR. Analytical methods used in milk recording - Version n 1 13/10/2005 Page 1/6
2 I - INTERNATIONAL REFERENCE METHODS FAT Gravimetric method (Röse-Gottlieb) ISO 1211 IDF 1 AOAC (IDF-ISO-AOAC-Codex) Gravimetric method (modified Mojonnier) AOAC (IDF-ISO-AOAC) PROTEIN: Titrimetric method (Kjeldahl) ISO 8968 IDF 20 AOAC 991:20 (IDF-ISO-AOAC) AOAC 991:21 AOAC 991:22 (IDF-ISO-AOAC) AOAC 991:23 (IDF-ISO-AOAC-Codex) CASEIN: Titrimetric method (Kjeldahl) ISO IDF 29 AOAC AOAC AOAC AOAC LACTOSE: HPLC method is foreseen to provide the reference to routine methods by ISO IDF and its international standardisation is underway (ISO CD IDF 198). In the meantime, standardised methods as referred to in Part II, Other methods can be used. UREA: Differential ph-method (Reference method) ISO IDF 195 SOMATIC CELL COUNT: Microscope method (Reference method) ISO IDF 148 Analytical methods used in milk recording - Version n 1 13/10/2005 Page 2/6
3 II - OTHER METHODS (SECONDARY REFERENCE) FAT: Butyrometric method (Gerber) ISO 2446 AOAC Babcock AOAC PROTEIN: Dye-binding (Amido Black) ISO 5542 IDF 98 AOAC (IDF-ISO-AOAC) Dye-binding (Orange 12) AOAC Dumas method ISO LACTOSE: Enzymatic ISO 5765 IDF 79 AOAC Gravimetric AOAC Polarimetric AOAC Under standardisation: High Performance Liquid Chromatography ISO CD IDF 198 Differential ph-method ISO WD IDF (working draft) Analytical methods used in milk recording - Version n 1 13/10/2005 Page 3/6
4 III - STANDARDIZED ROUTINE METHODS FAT: Automated turbidimetric I AOAC Automated turbidimetric II AOAC PROTEIN: Automated dye-binding (Amido Black) AOAC (FIL-ISO-AOAC) FAT-PROTEIN-LACTOSE: Mid infra red (MIR) spectrometric ISO 9622 IDF 141 AOAC UREA: (taken into account with the underway revision of ISO 9622 IDF 141) SOMATIC CELL COUNT: Electronic particle counter (Coulter Counter) (no longer taken into account with the underway revision of ISO IDF 148) Fluoro-opto-electronic method (Rotating disk) ISO IDF 148 AOAC Fluoro-opto-electronic method (Flow cytometry) (taken into account with the underway revision of ISO IDF 148) Analytical methods used in milk recording - Version n 1 13/10/2005 Page 4/6
5 IV - INSTRUMENTAL ROUTINE METHODS USED IN ICAR COUNTRIES (List drawn up with answers to ICAR questionnaires of 1994 and 1996, and supplemented with new validated analysers) FAT: Turbidimetric method: MilkoTester (Foss Electric, DK) FAT and PROTEIN: Turbidimetric/Dye-binding: MTA-PMA (Foss Electric, DK) FAT, PROTEIN (and LACTOSE): - Mid infra-red spectrometry: * Milkoscan (Foss Electric,DK) : 102, 103, 104, 104 (A/B) 133 A, 133 B, 134 (A/B) 203 A, 203 B, (A or B), 605 (A or B) Series 4000 (A or B) FT 120 (FTIR) FT 6000 (FTIR) * Multispec (Multispec,UK): MK 1 MK 2 Micro-null * Bentley (Bentley, USA): (A or B) * Lactoscope (Delta Instruments): 300, 550, 750 Filter Automatic 200 Filter Automatic 400 FTIR Auto 400 * Aegys (Anadis Instruments, F): MI 600 (FTIR) UREA: - Colorimetric methods: * 1-4 paradimethylaminobenzaldehyde method (DMAB) * Diacetyl monoxime method (DAM) - Automated enzymatic methods: Analytical methods used in milk recording - Version n 1 13/10/2005 Page 5/6
6 - Mid Infra-Red Spectrometry : * Conductimetry : Beckmann, BUN Analyser * Differential ph-metry : Eurochem, CL 10 Hamilton, E.F.A. * UV-photometry : Flow injection analysis (FIA). * Visible-photometry : Chemspec 150 (Bentley, USA) Skalar Segmented flow analysis * Milkoscan (Foss Electric,DK) 4000 FT 120 (FTIR) FT 6000 (FTIR) * Lactoscope (Delta Instruments): FTIR Auto 400 SOMATIC CELL COUNT: - Particle counting : * Coultronic (UK): Coulter Counter - Fluoro-opto-electronic : >Disk cytometry : * Foss Electric (DK): Fossomatic 90, 180, 215, 250, 360, 400 > Flow cytometry : * Anadis (F): Somatic Cell Counter 300, 500 * Bentley (USA): Somacount 150, 300, 500 * Chemunex (D): Partec CA 11 * Delta Instruments (NL): Somascope MKII Manual MKII Auto 200 MKII Auto 400 * Foss Electric (DK): Fossomatic 5000 Analytical methods used in milk recording - Version n 1 13/10/2005 Page 6/6
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