EXOVA (Ireland) Ltd.

Transcription

1 EXOVA (Ireland) Ltd. Glanmire Industrial Estate, Glanmire, Co. Cork. Testing Laboratory Registration number: 183T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 27:02:2007 Date of last renewal of accreditation: 21:02:2012 Expiry date of this certificate of accreditation: 27:02:2017 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Dr Adrienne Duff Chairperson: Mr Tom O Neill Issued on 21 February 2012 Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. The INAB is a signatory of the European co-operation for (EA) Testing Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement. Edition 22, 18/01/ T Page 1 of 33

2 Schedule of (Annex to Certificate) EXOVA (IRELAND) LTD Microbiology and Chemical Testing Laboratory Initial Registration Date : 27-February-2007 Postal Address: (Address of other locations as they apply) Glanmire Industrial Estate Glanmire Co Cork Telephone: +353 (21) Fax: +353 (21) dan.healy@exova.com Contact Name: Mr Dan Healy Facilities: Public testing service Edition 22, 18/01/ T Page 2 of 33

3 Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 22, 18/01/ T Page 3 of 33

4 Microbiological Testing Laboratory Pharmacological Tests on Pharmaceuticals Endotoxin Tests Purified Water Determination of Bacterial Endotoxin (LAL) In house method MT127 based on EP Microbiological Tests In house methods on Pharmaceuticals.12 Microbial Counts Total Aerobic Microbial Count MTG32 based on USP (61)/EP Total Yeast and Mould count MTG32 based on USP(61)/EP Staphylococcus aureus MTG33 based on USP(62)/EP Pseudomonas aeruginosa MTG33 based on USP(62)/EP Salmonella spp MTG33 based on USP(62)/EP Escherichia coli MTG33 based on USP (62)/EP Bile Tolerant Gram negative MTG33 based on USP(62)/EP Bacteria Edition 22, 18/01/ T Page 4 of 33

5 Microbiological Testing Laboratory Microbial Tests on Foods Dairy Products Enumeration of coliforms - MPN technique at 30 C In house methods MT371 based on ISO 4831:2006 Enumeration of coliforms - Colony count technique at 30 C MT374 based on ISO4832:2006 Detection of Salmonella spp Yeasts and Moulds Enumeration of Coagulase Positive Staphylococci - including Staph. aureus GMM001 based on ISO6579:2002 MT023 based on IDF94 ISO 6611:2004 MT384 based on ISO :1999 Micro-organisms - Colony count at 30 C GMM004 based on ISO4833:2013 Enumeration of Cl. Perfringens Enumeration of presumptive E.Coli by MPN MT360 based on ISO 7937:2004 MT128 based on ISO 7251:2005 Edition 22, 18/01/ T Page 5 of 33

6 Microbiological Testing Laboratory Microbial Tests on Foods Dairy Products Enumeration of -glucuronidase positive E. coli - Colony count at 44ºC In house methods GMM007 based on ISO :2001 Detection of Enterobacter sakazakii (C.sakazakii) MTG31 based on ISOTS 22964:2006 Enumeration of Enterobacteriaceae MT388 based on ISO :2004 Enumeration of Listeria spp and L. monocytogenes MTE97 based on ISO :1998 Amd 1:2004 Detection of Listeria spp and L.monocytogenes p MTE96 based on ISO :1996 Amd 1:2004 Detection of Salmonella spp. Documented in-house method CKBGM001 using Solus DS2 ELISA system Detection of Listeria spp and L.moncytogenes Documented in-house method CKBGM002 using Solus DS2 ELISA system Detection of Clostridium perfringens MTG34 in-house method based on ISO 7937:2004 using RMP and Crossley milk Edition 22, 18/01/ T Page 6 of 33

7 Microbiological Testing Laboratory Microbial Tests on Foods Dairy Products Enumeration of Bacillus cereus and other Bacillus spp In house methods MT568 based on ISO7932:2004 Enumeration of anaerobic mesophilic spores CKAM029/IHM/M/BS :1986 Enumeration of lactic acid bacteria CKAM026/IHM/M/ISO 15214:1998 Enumeration of Pseudomonas spp CKAM020/IHM/M/ISO 13720:2010 Detection of Enterobacteriaceae MT389 documented in-house method based on ISO :2014 Edition 22, 18/01/ T Page 7 of 33

8 Microbiological Testing Laboratory Microbial Tests on Foods Cereal and bakery products Colony count at 30 0 C In house methods GMM002 based on ISO 4833: Nuts and nut products Enumeration of coliforms MT374 based on ISO (colony count 37 C ) 4832: Meat and Meat products, game and Enumeration of coliforms MPN MT371 based on ISO poultry technique 4831:2006*.02 Eggs and Egg products Detection of Salmonella spp GMM001 based on ISO 6579: Fish Crustaceans & Enumeration of Coagulase MT384 based on ISO molluscs Positive Staphylococci - 1:1999 including Staph. aureus.08 Vegetables and Enumeration of Cl.perfringens MT360 based on ISO vegetable products 7937:2004 Enumeration of MT388 based on ISO Animal Feeds Enterobacteriaceae 2:2004 Enumeration of Yeasts and GMM012 based on ISO.19 Additives to Food Moulds : Pet Foods Enumeration of presumptive MT128 based on ISO E.coli by MPN 7251:2005*.21 Other Food products *excluding 811,.06,.09, (Pizza).10,.13,.14,.15,.17 Edition 22, 18/01/ T Page 8 of 33

9 Microbiological Testing Laboratory Microbial Tests on Foods contd. Soups and Broths Enumeration of β-glucuronidase positive E.coli colony count at 44º C In house methods GMM007 based on ISO : Herbs and Spices Enumeration of Listeria spp and MTE97 based on ISO L.monocytogenes 2:1998 Amd 1: Non Alcoholic Beverages Detection of Listeria spp and MTE96 based on ISO Ices and Deserts L.monocytogenes 1:1996 Amd 1: Cocoa,Tea,Coffee.15 Confectionary.17 Prepared Dishes Edition 22, 18/01/ T Page 9 of 33

10 Microbiological Testing Laboratory 811 Microbial Tests on Foods contd.. Enumeration of Bacillus cereus and other Bacillus spp In house methods MT568 based on ISO7932:2004 Enumeration of anaerobic mesophilic spores CKAM029/IHM/M/BS :1986* *excluding ,.09,.10,.13,.14,.15,.17 Enumeration of lactic acid bacteria CKAM026/IHM/M/ISO 15214:1998 Enumeration of Pseudomonas spp CKAM020/IHM/M/ISO 13720:2010 Edition 22, 18/01/ T Page 10 of 33

11 Microbiological Testing Laboratory 811 Microbial Tests on Foods contd.. Detection of salmonella spp. Documented in-house method CKBGM001 using Solus DS2 ELISA system Detection of Listeria sp and L.monocytogenes Documented in-house method CKBGM002 using Solus DS2 ELISA system Oils and Fats Detection of Salmonella spp. Detection of Salmonella spp. Detection of Listeria sp and L.monocytogenes Enumeration of Enterobacteriaceae CKBGM001 Documented inhouse method based on Solus DS2 ELISA system GMM001 based on ISO6579:2002 CKBGM002 Documented in-house method based on Solus DS2 ELISA system MT388 based on ISO :2004 Enumeration of Cl. Perfringens MT360 based on ISO 7937:2004 Edition 22, 18/01/ T Page 11 of 33

12 Microbiological Testing Laboratory Microbial tests on other materials Sludge Compost Detection of Salmonella spp. Detection of Salmonella spp. Enumeration of B-glucuronidase +ve E.coli Colony count 44oC CKBGM001 Documented inhouse method based on Solus DS2 ELISA system GMM001 based on ISO6579:2002 GMM007 based on ISO :2001 Edition 22, 18/01/ T Page 12 of 33

13 Microbiological Testing Laboratory Microbial Tests on Foods Foodstuffs intended for special nutritional uses Detection of salmonella spp. Detection of Listeria sp and L.monocytogenes Documented in-house method CKBGM001 using Solus DS2 ELISA system Documented in-house method CKBGM002 using Solus DS2 ELISA system Enumeration of coagulase positive Staphlococci incl.s.aureus MT384 based on ISO6888-1:1999 Enumeration of B-glucuronidase +ve E.coli Colony count 44oC GMM007 based on ISO :2001 Enumeration of Listeria spp. and L.monocytogenes MTE97 based on ISO :1998 Amd1:2004 Enumeration of Enterobacteriaceae MT388 based on ISO :2004 Colony count at 30 0 C GMM002 based on ISO 4833:2013 Enumeration of Yeasts and Moulds GMM012 based on ISO :2008 Edition 22, 18/01/ T Page 13 of 33

14 Microbiological Testing Laboratory Testing of Surfaces in Abattoirs Meat Surfaces Product Contact Surfaces Colony count at 30 0 C Detection of Salmonella spp Detection of Listeria spp and L. monocytogenes In house methods GMM002 based on ISO 4833: 2013 GMM001 based on ISO 6579:2002 MT095 In house validated method. Enumeration of coliforms - colony count technique at 37ºC MT319 method based on ISO 4832:2006 Enumeration of Coagulase Positive Staphylococci - Including Staph. aureus MTA92 based on ISO :1999 Enumeration of presumptive Enterobacteriaceae GMM003 based on ISO :2004 Detection of salmonella spp. Documented in-house method CKBGM001 using Solus DS2 ELISA system Dectection of Listeria sp and L.monocytogenes Documented in-house method CKBGM002 using Solus DS2 ELISA system Edition 22, 18/01/ T Page 14 of 33

15 Microbiological Testing Laboratory Microbiological Tests for Factory Hygiene Purposes Surfaces Colony count at 30 0 C Detection of Salmonella spp Detection of Enterobacter sakazakii (C.sakazakii) In house methods GMM002 based on ISO 4833: 2013 GMM001 based on ISO 6579:2002 MTG31 based on ISO TS 22964:2006 Detection of Listeria spp Enumeration of coliforms by colony count technique at 37ºC MTE96 based on ISO :1996 Amd1:2004 MT319 based on ISO 4832:2006 Enumeration of Coagulase Positive Staphylococci - Including Staph. aureus MTA92 based on ISO :1999 Enumeration of presumptive Enterobacteriaceae GMM003 based on ISO :2004 Edition 22, 18/01/ T Page 15 of 33

16 Microbiological Testing Laboratory Microbiological Tests for Factory Hygiene Purposes Surfaces Enumeration of B-glucuronidase +ve E.coli Colony count 44oC GMM007 based on ISO :2001 Detection of Salmonella spp. CKBGM001 Documented inhouse method based on Solus DS2 ELISA system Detection of Listeria spp. and L.Monocytogenes MT095 In house validated method Detection of Listeria spp. and L.Monocytogenes Documented in-house method CKBGM002 using Solus DS2 ELISA system Detection of Enterobacteriaceae MT389 documented in-house method based on ISO :2014 Edition 22, 18/01/ T Page 16 of 33

17 Microbiological Testing Laboratory Microbiological Tests for Factory Hygeine Purposes Water Heterotrophic plate count pour plate 0 C,@ C MTO50 based on APHA 2012: 9215:B Enumeration of Pseudomonas aeruginosa MTG38 based on The Microbiology of drinking water 2010 part 8 Edition 22, 18/01/ T Page 17 of 33

18 Microbiological Testing Laboratory Water including effluent Bacteriological condition of potable waters Enumeration of coliforms and Escherichia coli by membrane filtration In house methods MT420 based on ISO : Bacteriological Enumeration of total coliforms MTC12 based on condition of sewage & E.coli The Microbiology of Drinking Water 2009 Part 4(d).14 Bacteriological condition of trade Colony count MTC92 based on ISO 22 0 C 37 0 C 6222: Bacteriological Enterococci (intestinal) MT377 based on ISO condition of swimming 2:2000 pools and spas Clostridium perfringens (including spores) MTD73 based on 98/83/EC.16 Bacteriological Directive condition of environmental waters Detection of Sulfite MT378 based on ISO - reducing spores 6461/1:1986 Enumeration of Coagulase MT185 based on APHA 2012: Positive Staphylococci B.6 Including Staph. aureus Edition 22, 18/01/ T Page 18 of 33

19 Microbiological Testing Laboratory 870 Water including Effluent - Contd. Enumeration of Pseudomonas aerginosa Heterotrophic plate count - pour plate C Detection of Salmonella spp. MTG38 based on The Microbiology of Drinking Water 2010 Part 8 MTO50 based on APHA : B CKBGM001 Documented inhouse method based on Solus DS2 ELISA system Detection of Salmonella spp. GMM001 based on ISO6579: Bacteriological Heterotrophic Plate Count - MT050 based on APHA 2012 condition of industrial Pour Plate Method at 35ºC 9215B waters.99 Purified water.13 Bacteriological Enumeration of Total Coliform Documented in house condition of sewage MPN method MT048 based on APHA B.14 Bacteriological condition of trade Enumeration of Faecal Coliform Documented in house wastes MPN method MT049 based on APHA E.1.16 Bacteriological condition of environmental waters Edition 22, 18/01/ T Page 19 of 33

20 Chemical Testing Laboratory 751 Foods In house methods.01 Dairy Products Fat Content Liquid Milk FT242 based on IDF:1D: % - Mojonnier/Rose Gottlieb Fat Content Dried Milk FT242 based on IDF: 9: 2008 Products % - Mojonnier/Rose Gottlieb Water Content - Dried Milk and FT267 based on Dried Cream % IDF:26A: Oven Drying at C Protein Content - Dairy FT001 based on Products (Nx6.38) IDF20-3:2004 Kjeldahl % Vitamin A Content of Milk CT140 HPLC Method based Powders on ISO : ,000 iu/100g Non Protein Nitrogen FT419 based on IDF20 Part % TCA extract and Kjeldahl Edition 22, 18/01/ T Page 20 of 33

21 Chemical Testing Laboratory 751 Foods In house method.03 Meat & Meat Products, Added Water 0-50% FT 577 calculation, based on game and poultry EU Directive 2001/101 Apparent Total Meat Content % Excess fat, 0-20% Connective tissue, 0-20% Apparent fish content,10-100% Edition 22, 18/01/ T Page 21 of 33

22 Chemical Testing Laboratory Foods Dairy Products Meat and Meat Products, game and poultry products Fish Products Soups and Sauces Cereal & bakery products Fruit and vegetables Non-alcoholic beverages (Flavoured Waters) Ices and deserts Confectionary Prepared dishes Foodstuffs intended for special nutritional uses Pet foods Total solids / Moisture % Protein (N x Factor) % Nitrogen % Protein % Ash % Chloride Content and Calculation of NaCl % Carbohydrate Total Content Calculated 0-99% Carbohydrate Available Content (by difference) 0-99% In house methods: FT007 (Oven Dry at 102 C) FT001 Kjeldahl, based on IDF 20-3 : 2004 CKAM081 using Leco Analyser FT117, based on SI incineration in a muffle furnace FT316, based on ISO 5943:2006 Argentometric titration with Potentiometric end point determination FT218, based on EU food labelling Regulation 1996, as amended 2009 FT218, based on EU food labelling Regulations 1996, as amended 2009 Energy (Calculated) FT389, based on EU food labelling Regulations 1996, as amended 2009 Edition 22, 18/01/ T Page 22 of 33

23 Chemical Testing Laboratory 751 Foods contd. All categories Type of test/properties Determination of Nitrate as NO to 5000 mg/kg 3.4 to 6850 mg/kg as NaNO 3 Determination of Nitrite as NO to 5000 mg/kg 0.75 to 7500 mg/kg as NaN0 2 FT438/ISO /IDF189-3 FIA Cadmium Reduction Vitamin A content 30-4,000,000I iu/100g 30-20,000 iu/100g for Dairy CT140: HPLC Method, based on ISO :2009 Products Vitamin C content mg/100g FT110 based on AOAC Fat (total) % GCM 001 (NMR) Total Solids / Moisture 12-92% AM188/IHM/C(microwave), in house validated method Fatty Acid Profile % includes saturates: - Monounsaturates - Polyunsaturates CT671 GLC Method based on ISO and ISO Sugars- lactose, sucrose, fructose, glucose, maltose, total sugars (calculated) Liquid foods ( %) Other sample types (0.1-90%) CT712 based on J.AOAC 62, 1292,1979, HPLC method Edition 22, 18/01/ T Page 23 of 33

24 Chemical Testing Laboratory 751 Foods contd. In house methods All categories Total Phosphorous % FT014 Molybdovanadate Colorimetric method based on SI Determination of Sodium and calculation of salt (Na x 2.5) 3mg/kg - 60% Determination of Potassium 3mg/kg - 60% Determination of Calcium 30mg /kg - 40 % Determination of Magnesium 3mg/kg - 50 % FT022 Atomic absorption method FT022 Atomic Absorption method FT013 Atomic Absorption method based on SI FT013 Atomic Absorption method based on SI Edition 22, 18/01/ T Page 24 of 33

25 Chemical Testing Laboratory Foods contd. Other Brine Powdered Brine Determination of Sodium Nitrate and Sodium Nitrite % In house methods FT438/ISO /IDF189-3 FIA Cadmium Reduction. 761 Agricultural Products Total solids/moisture % FT007 (Oven Dry at 102 C), and Materials based on SI Cereal, Grains and by- Protein (N x Factor) % FT001 Kjeldahl, based on products IDF 20-3 : Oil seeds and By Nitrogen % CKAM081 using Leco Products Protein % Analyser.03 Stockfoods Total Phosphorous % FT014 Molybdovanadate Colorimetric method based.04 Vitamins in on SI stockfoods Determination of Sodium and FT022 Atomic absorption.99 Other agricultural calculation of salt (Na x 2.5) method products 3mg/kg - 60% Premixes Additives Determination of Potassium FT022 Atomic Absorption 3mg/kg - 60% method Determination of Calcium FT013 Atomic Absorption 30mg /kg - 40 % method based on SI Determination of Magnesium FT013 Atomic Absorption 3mg/kg - 50 % method based on SI Edition 22, 18/01/ T Page 25 of 33

26 Chemical Testing Laboratory Fats, oils and waxes Animal sources Insoluble Impurities In house gravimetric method FT182, based on ISO 663:2007 and AOCS Ca 3a-46: Agricultural Products In house methods and Materials.01 Cereal, Grains and by- Ash % FT117, based on SI products incineration in a muffle furnace.03 Stockfoods Chloride Content and Calculation FT316, based on ISO5943: Vitamins in stockfoods of NaCl % Argentometric tit. with Potentiometric end point Carbohydrate Total Content FT218, based on EU food Calculated 0-99% labelling regulations 1996 Carbohydrate Available Content FT218, based on EU food (by difference) 0-99% labelling regulations 1996 Energy (Calculated) FT389, based on EU food labelling regulations 1996 as amended 2009 Fat (total) % GCM001 (NMR) - Meat & Bone Meal Moisture 12-92% AM188/IHM/C (microwave), in house validated method Vitamin A Content CT140 HPLC Method, based on 30-4,000,000 iu/100g IDF Edition 22, 18/01/ T Page 26 of 33

27 Chemical Testing Laboratory 781 Constituents of the Environment In house methods.32 Sediments Potassium mg/kg FT022 Atomic Absorption,.33 Solid waste based on SI Kjeldahl Nitrogen % FT001 based on IDF 20-3 : 2004 Nitrogen % CKAM081 using Leco Protein % Analyser Total Solids % FT007 oven dry at 102º C Fat % GCM001 (NMR) in house validated method Total Phosphorus FT014 based on mg/kg Molybdovanadate Colorimetric Method Edition 22, 18/01/ T Page 27 of 33

28 Chemical Testing Laboratory Waters Waters for potable and domestic purposes Turbidity NTU Electrical Conductivity at 20 0 C µs/cm ph (ph units) 4 10 Total Alkalinity as CaCo mg/l In house methods; ET041 based on APHA B ET056/APHA 2012:2510:B Meter method ET124 based on APHA 2012:4500H:B ET058 based on APHA 2012: 2320B Calcium mg/l Chloride mg/l Magnesium mg/l Nitrate mg/l as N (by calculation) Nitrite mg/l as N Potassium mg/l Fluoride mg/l ET254 based on APHA 2012: 3111B ETC98 based on APHA 2012, 4110B ion chromatography ET254 based on APHA B ET235 based on APHA 2012 :4500:NO 3 :I FIA/Colorimetric ET043 based on CMWT BDH nd Ed ET252 based on APHA 2012: 3111B ETC98 based on APHA 2012 : 4110 B Ion chromatography Edition 22, 18/01/ T Page 28 of 33

29 Chemical Testing Laboratory Waters Waters for potable and domestic purposes Sodium mg/l Sulphate mg/l ET252 based on APHA 2012: 3111B ETC98 based on APHA 2012:4110B ion chromatography Total Anions (by calculation) ET060 based on APHA 2012:2330 Total Cations (by calculation) ET060 based on APHA 2012:2330 Total Dissolved Solids (by calculation) ET060 based on APHA 2012:2330 Total Hardness (by calculation) ET057 based on APHA 2012:2340B Ammonia/Ammonium mg/l as N ET038 based on MEWAM 1981 FIA/Colorimetric Method Edition 22, 18/01/ T Page 29 of 33

30 Chemical Testing Laboratory 766 Surface Water Ammonia/Ammonium ET038 based on MEWAM mg/l as N 1981 FIA/Colorimetric Method Fluoride mg/l ETC98 based on APHA 2012 : 4110 B Ion chromatography Electrical Conductivity ET056/APHA 2012:2510:B 5 1,400 µs/cm Meter method Total Nitrogen 1 30 mg/l as N ETG03 (Ganimede) Based on ISO : Bore Water Electrical Conductivity ET056/APHA 2012:2510:B 5 1,400 µs/cm Meter method 766 Waters Ammonia (Total) In house method.04 Sewage mg/l as N ET038 based on MEWAM.08 Waste water mg/l as N 1981 FIA/Colorimetric treatment effluent Method (WWTP effluent).05 Trade Wastes mg/l as N Biochemical Oxygen Demand ET066 based on APHA ,000 mg/l (Sewage) :5210 :B DO Meter Method 2-40,000 mg/l (Trade Waste) Edition 22, 18/01/ T Page 30 of 33

31 Chemical Testing Laboratory 766 Chemical Oxygen Demand ET068 based on APHA 2012, 5-40,000 mg/l (Sewage) 5220D Colormetric.04 Sewage 5-60,000 mg/l (Trade Waste).05 Trade Waste Chloride 5-1,000 mg/l as Cl ET244 based on APHA 2012 :4500 CL :D Titrimetric.08 Waste water Method treatment effluent Oil Fat & Grease ET084 based on USEPA 1664 (WWTP) mg/l Gravimetric Method Suspended Solids (MLSS) ET042 based on APHA 2012: ( ºC) 2540:D 3-10,000 mg/i Total Phosphorus Gravimetric Method mg/l as P ETG01 (Ganimede) based on ISO 6878:2004 Soluble reactive phosphorus ETGO2 (Ganimede) (MRP) mg/l as P based on ISO 6878:2004 Chloride mg/l (Sewage) 1-5,000 mg/l (Trade Waste) ETC98 based on APHA Sulphate 2012 : 4110 B Ion mg/l (Sewage) chromatography 1-5,000 mg/l (Trade Waste) Edition 22, 18/01/ T Page 31 of 33

32 Chemical Testing Laboratory Waters Sewage Trade Wastes Waste water treatment effluent (WWTP) Electrical 25 0 C 5-1,400 µs/cm (Sewage) 5-10,000 µs/cm (Trade Waste) ph (ph units) 4-10 Turbidity NTU In house method ET056 based on APHA 2012 :2510:B Meter method ET124 based on APHA 2012: 4500H : B Meter method ET041 based on APHA 2012: 2130 : B Total Nitrogen mg/l as N ETG03 (Ganimde) based on ISO :1998 Total Kjeldahl Nitrogen mg/l as N by calculation using results from method ETG03 and ET235 ETG 19, based on APHA 2012:4500 Total Oxidised Nitrogen mg/l Nitrite as N mg/l Nitrate (calculated by difference) mg/l as N ET235 based on APHA 2012: 4500:NO3:I FIA/Colorimetric Edition 22, 18/01/ T Page 32 of 33

33 Chemical Testing Laboratory Sewage WWTP influent Trade Waste Fluoride mg/l ETC98 based on APHA 2012 : 4110 B Ion Chromatography.08 Waste water treatment effluent (WWTP) 766 Waters Conductivity µs/cm In house method ETD13 EP 1927 and USP.99 Other Waters <645> Purified water ph ET462 EP and USP <791> Chloride mg/l Fluoride mg/l Sulphate mg/l ETC98 based on APHA 2012 : 4110 B Ion chromatography Total Organic Carbon (TOC) µg/l ETE and USP <643> Turbidity NTU ET041 based on APHA 2012:2130B Edition 22, 18/01/ T Page 33 of 33