Animal Care and Resource Center (ACRC) Facility infrastructure.
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1 Animal Care and Resource Center (ACRC) Facility infrastructure
2 Animal Care and Resource Center (ACRC) Facility infrastructure The ACRC currently houses four species of laboratory animals: Mice (around 140 strains which include inbred, out bred and transgenic lines), Rats (around 10 strains including out bred stocks like Wistar, Sprague Dawley, Long Evans and several transgenic rat strains), Xenopus laevis and Zebra fish. All the animal rooms have a controlled environment with 20-22'c temperature, relative humidity, 0.3 micron HEPA filtered air supply at ACPH and 14/10 light/dark cycle maintained. Most of the mouse colonies and some rat colonies are housed in Individually Ventilated Caging (IVC) systems. The ACRC maintains most rodent rooms under Specific Pathogen Free (SPF) conditions. Health monitoring of the animal colonies is done every quarter. Within the ACRC, The Mouse Genome Engineering Facility (MGEF) provides services and training to generate genetically modified mouse models, and access to cryopreservation and in vitro fertilization technologies.
3 ACRC- Animal Facility Vivarium Layout NCBS campus map Animal Facility Vivarium Vivarium 1: old facility Rats (SPF level1 and SPF level2), future ZebraFish, current Xenopus Vivarium 2: New facility High Barrier Mouse Facility (SPF.1& SPF.2), Vivarium 3: ground floor of SLC - future ABSL-3 mouse holding room for TB infected animals Vivarium 4: current Mouse Behavior Room / mice in transit room in B38 lab low barrier animal holding room
4 Animal Facility Vivarium Layout Vivarium -2 (May 2016) Zebrafish V2 = 2093 m 2 Max 5932 mouse IVCs As of May 2016: 2100 SPF1 IVC cages (including 280 quarantine IVCs) 1960 SPF2 IVC cages 100 V4 conventional cages 200 mouse ABSL2 small IVC cages 4
5 Vivarium -2 : High Barrier SPF facility Air Shower Barrier segregation Example of mice SPF animal holding room with IVC system Procedure Room
6 Vivarium -2 : High Barrier SPF facility
7 Renovated Vivarium 1 (V 1)
8 Vivarium 1 (V1): Total floor area of 1494 Sqm.(3 floors 2590 rat cages) 7 rooms - Highest Barrier (SPF) rat colonies (1465 cages). 9 rooms Medium Barrier rat colonies (1125 cages). 8 rooms Experimental rat rooms. 2 rooms Quarantine facility. 2 rooms Rat procedures. 2 rooms Rat ABSL2 facility (60 cages). 3 rooms future Zebrafish colonies 1 room Xenopus laevis colony (8 tanks).
9 The Mouse Genome Engineering Facility Facility equipment: Different types of glass capillary pullers / grinders / forges to make all types of required needles Two Microinjections setups with teaching camera and monitor - Olympus Modulation Contrast microscope / Narishige micromanipulators / Eppendorf injectors - Nikon Modulation Contrast microscope / Eppendorf motorized micromanipulators, Piezzo drill, and injectors.
10 The Mouse Genome Engineering Facility Facility equipment: Different types of glass capillary pullers / grinders / forges to make all types of required needles Two Microinjections setups with teaching camera and monitor - Olympus Modulation Contrast microscope / Narishige micromanipulators / Eppendorf injectors - Nikon Modulation Contrast microscope / Eppendorf motorized micromanipulators, Piezzo drill, and injectors. Embryo transfer / live microsurgery platform with teaching camera and monitor
11 The Mouse Genome Engineering Facility Facility equipment: Different types of glass capillary pullers / grinders / forges to make all types of required needles Two Microinjections setups with teaching camera and monitor - Olympus Modulation Contrast microscope / Narishige micromanipulators / Eppendorf injectors - Nikon Modulation Contrast microscope / Eppendorf motorized micromanipulators, Piezzo drill, and injectors. Embryo transfer / live microsurgery platform with teaching camera and monitor C02 incubators / trigaz incubators 200 SPF1 (specific pathogen free environment) IVC cages to house oocyte producing animals of different genetic backgrounds, pseudo-pregnant female production, male studs, vasectomized males, recovery of engineered animals.
12 The Mouse Genome Engineering Facility Facility equipment: Different types of glass capillary pullers / grinders / forges to make all types of required micro-needles
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