Reagents for ES cell culture and ips cell induction!!

Transcription

1 Reagents for ES cell culture and ips cell induction!! Cell Research Maintain ES & ips cells in an undifferentiated state Improve reprogramming efficiency A TGF-β RI Kinase Inhibitor IV ALK4, ALK5, ALK7 selective inhibitor Used to culture rat ips cells uniformly in an undifferentiated state for a long period of time. [Li, W.,et al.: Cell Stem Cell, 4, 16 (2009)] Adrenocorticotropic Hormone (1-24) (Human) ACTH Used to form single and undifferentiated mouse ES cell colonies. [Ogawa, K.,et al.: Genes Cells, 9, 471 (2004)] (±) BayK8644 L type Ca²+ Channel Agonist When used with Oct3/4 and Klf4, BayK8644 is able to culture ips cells more efficiently. [Shi, Y., et al.: Cell Stem Cell, 3, 568 (2008)] BIX01294 Histone Methyltransferase inhibitor BIX01294 enhances reprogramming efficiency of neural progenitor cells to the same levels as when Oct4, Klf4 were introduced to somatic cells for generation of ips Cells. [Shi, Y., et al.: Cell Stem Cell, 2, 525 (2008)] [Shi, Y., et al.: Cell Stem Cell, 3, 568 (2008)] Bromoindirubin-3 -oxime BIO/GSK-3 Inhibitor IX Can maintain human and mouse ES cells in an undifferentiated state without feeder cells 1). Assay: 97.6% (HPLC: Data of first lot) Soluble in DMSO (1mg/ml confirmed) C16H10BrN302= ) [Sato, N., et al.: Nature Med., 10, 55 (2004)] CHIR99021 (CT99021) High selective GSK-3 Inhibitor CHIR99021 is a GSK-3 b (Glycogen Synthase Kinase3b) inhibitor and is currently known as the most selective inhibitor. A recent study has shown that mouse ES cells can be cultured efficiently in a medium containing PD184352, SU5402 and this product, CHIR ). Another study has also shown that CHIR99021 increases the reprogramming efficiency from mouse neural stem cells to ips cells 3). 2) [Ying, QL., et al.: Nature, 453, 519 (2008)] 3) [Silva J, et al.: PLoS Biol, 6, 2237 (2008)] For CellbIology CAS CAS CAS CAS CAS C 16 H 10 BrN 302 = CAS C 22 H 18 Cl 2 N 8 = mG 10mG 2mG Page 1/6

2 DNA Methyltransferase Inhibitor Controls in cell proliferation through low cytotoxicity. [Tsumura, A., et al.: Genes Cells, 11, 805 (2006)] RG108 For Genetic Research CAS mG Kenpaullone GSK-3, CDKs Inhibitor Can be a substitute for Klf4 when manufacturing ips cell 4). Can be able to grow efficiency reprogramming when used combination with Yamanaka factors (Oct3/4, Sox2, Klf4, c-myc) 4). Assay: 100.0% (HPLC: Data of first lot) Soluble in DMSO (1mg/ml confirmed) 4) [Lyssiotis, CA., et.al.: Proc. Natl. Acad. Sci. USA., 106, 8912 (2009)] CAS C 16 H 11 BrN 2 O = PD MAPK Inhibitor Used to maintain self-propagation efficiency of ES Cell with CHIR ). Can be able to grow dramatically efficiency reprogramming to Human ips Cells when used with SB It also promotes the speed 5). Assay: 97.0% (HPLC) Soluble in DMSO (about 20mg/ml) [Related Products] 2) [Ying, QL., et al.: Nature, 453, 519 (2008)] 5) [Lin, T.,et al.: Nature Methods., 6, 2237 (2009)] CAS C 16 H 14 F 3 IN 204 = ug: micrograms PD MAPK Inhibitor Promotes self propagation of embryonic stem cells. [Burdon, T., et.al.: Dev. Biol., 210, 30 (1999)] [Hamazaki, T., et.al.: Mol. Cell. Biol., 26, 7539 (2006)] CAS SB MAPK Inhibitor Used to isolate and maintain embryonic stem cells. [Qi, X., et al.: Proc. Natl. Acad. Sci. USA., 101, 6027 (2004)] CAS SC-1 (Pluripotin) RasGAP, ERK1 Inhibitor Able to maintain mouse ES cells in an undifferentiated state even in a medium without LIF, feeder cells, and sera 6). Assay: 98.0% (HPLC) Soluble in DMSO (1mg/ml confirmed) 6) [Chen, S., et al.: Proc. Natl. Acad. Sci. USA., 103, (2006)] C 27 H 25 F 3 N 8 O 2 = CAS Page 2/6

3 SU5402 FGFR1 Inhibitor Efficiently cultures ES cell within its undifferentiated state when used along with CHIR99021 and PD [Ying, QL., et.al.: Nature, 453, 519 (2008)] CAS Sodium Butyrate Histone deacetylase Inhibitor N/A 25G Promotes self-propagation of embryonic stem cells. [Ying, QL., et.al.: Nature, 453, 519 (2008)] CAS G Valproic Acid Histone deacetylase Inhibitor 5G Enhances efficiency of ips cell induction by Oct4, Klf4 and Sox2. [Huangfu, D., et al.: Nature Biotechnol., 25, 1269 (2008)] CAS G Y ROCK Inhibitor Enhances survival rate after cryopreservation and cloning efficiency of human ES and ips cells. [Claassen, DA., et al.: Mol. Reprod. Dev., 76, 722 (2009)] [Martin-lbanez, R., et al.: Hum. Reprod., 23, 2744 (2008)] [Watanabe, K., et al.: Nature Biotechnol., 25, 681 (2007)] [Sakamoto, K., et al.: J. Pharamacol. Sci., 92, 56 (2003)] [Nishimaru, K., et al.: J. Pharamacol. Sci., 92, 424 (2003)] [Uehata, M., et al.: Nature, 389, 990 (1997)] <This is sold under license from Mitsubishi Tanabe Pharma Corporation.> Differentiation Induction Dedifferentiation of ES and ips cells AICAR AMPK Inhibitor 5-Amino-4-imidazolecarboxamide-1-beta-Dibofuranoside AICAAR inhibits differentiation to adipocyte and induces neural stem cell to astroglia. [Giri, S., et al.: Nutr. Metab (Lond), (2006)] [Zang, Y., et al.: J. Biol. Chem., 283, 6201 (2008)] Ciglitazone PPAR gamma Agonist In human mesenchymal stem cells, Ciglitazone induces differentiation to adipocytes and inhibit differentiation to osteoblasts [Benvenuti, S., et al.: J. Endocrinol. Invest., 30, RC )] CAS CAS mG Page 3/6

4 Differentiation Induction Dedifferentiation of ES and ips cells DAPT gamma-secretase Inhibitor IX Inhibits notch signaling pathway and induces differentiation of embryonic stem cell from embryoid body to neuron. [Nelson, BR., et al.: Dev. Biol., 304, 479 (2007)] [Crawford, TQ., et al.: Dev. Dyn., 236, 886 (2007)] Dexamethazone Regulates bone differentiation of mesenchymal stem cells. [Jaiswal, N., et al.: J. Cell. Biochem., 64, 295 (1997) LY phosphatidylinositol 3-kinase Inhibitor Induces differentiation of mouse ES cells to insulin-producing cells. Purmorphamine Hedgehog Signal Agonist Induces differentiation of mesenchymal stem cells and preosteoblastic cells to osteoblasts. [Wu, X., et al.: Chem. Biol., 11, 1229 (2004)] all-trans-retinoic Acid Induces differentiation to neuron. CAS CAS CAS CAS CAS mG 10mG 2 50mG 100mG 250mG Reversine Human A3 adenosine receptor Agonist Dedifferentiate myoblast to stem cells. [Chen, S., et al.: J. Am. Chem. Soc., 126, 410 (2004)] CAS SB n-hydrate ALK4, ALK5, ALK7 Inhibitor Can be able to grow dramatically efficiency reprogramming to Human ips Cells when used with PD It also promotes the speed 5). Promotes proliferation, differentiation and sheet formation of endothelial cells of ES cell origin 6). Assay: 97.0% (HPLC) Soluble in DMSO (1mg/ml ) 5) [Lin, T.,et al.: Nature Methods., 6, 2237 (2009)] 7) [Ogawa, K., et al.: J. Cell. Sci., 120, 55 (2007)] CAS C 22 H 16 N 4 O 3 = Page 4/4 Page 4/6

5 Differentiation Induction Dedifferentiation of ES and ips cells Spermine Induce differentiation to cardiac muscle cells CAS mG Trichostatin A Histone deacetylase Inhibitor Promotes differentiation to cardiac muscle cells. Oyama, T., et al.: J. Cell. Biol., 176, 329 (2007)] CAS Troglitazone PPAR gamma Agonist In human mesenchymal stem cells, Troglitazone induces differentiation to adipocytes and inhibit s differentiation to osteoblasts. [Benvenuti, S., et al.: J. Endocrinol. Invest., 30, RC26 (2007)] For Pharmacology CAS mG TWS119 PGSK-3 β Inhibitor XII In mouse ES cells, TWS119 induces neural differentiation. [Ding, S., et al.: Proc. Natl. Acad. Sci. USA., 100, 7632 (2003)] CAS Related Products G-MEM with L-Glutamine and Phenol Red For Cell Culture 500mG LIF, Human, recombinant, Culture Supernatant For Cell Culture 1mL 1mLx mmol/l L-Glutamine Solution (x100) For Cell Culture 100mL mmol/l L-Alanyl-L-Glutamine Solution (x100) For Cell Culture 100mL MEM Non-essential Amino Acids Solution (x100) For Cell Culture 100mL Fibroblast Growth Factor (basic) Human, recombinant bfgf / FGF2 There are NO signal peptide sequence (147a.a.) 25µG Fibroblast Growth Factor (basic) Human, recombinant bfgf/fgf2 There are signal peptide sequence (156a.a.) [Takahashi, K., et al.: Cell, 131,861 (2007)] 50µG 100µG Page 5/6

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