Phospho-Specific Antibodies for Stem Cell Signaling...

Transcription

1 Phospho-Specific Antibodies for Stem Cell Signaling. from Cell Signaling Technology Monitor the major signaling pathways in pluripotent and adult stem cells using phospho-specific antibodies from Cell Signaling Technology. All antibodies produced in-house and rigorously tested in applications including IF, IHC and flow cytometry. Technical support provided by the scientists who make the antibodies and know them best.

2 Embryonic and adult stem cells share many important signaling networks that define their resistance to apoptosis and their potential for proliferation and differentiation. As stem cells progress from totipotent to terminally differentiated cells, there are corresponding changes to their signal transduction pathways that regulate all aspects of cell biology. Major signaling mechanisms implicated in stem cell maintenance and differentiation include the Wnt/β-Catenin, Akt, Stat, TGF/BMP and Notch signaling pathways. Recent results suggest that some cancers may contain small populations of quiescent cancer stem cells that may give rise to drug resistance and recurrence of the disease following therapy. These results have important implications for the development and use of targeted cancer therapeutics. Cell Signaling Technology, the innovator in activation-state antibodies and related technologies, provides the highest quality reagents necessary to identify stem cells and to elucidate signal transduction pathways involved in stem cell maintenance and differentiation.

3 Wnt/β-Catenin Signaling Phospho-β-Catenin (Ser33/37/Thr41) Antibody H, M, R, Mk W, IHC, IC 9561 Phospho-β-Catenin (Thr41/Ser45) Antibody H, M, Mk W, IHC, IC 9565 Phospho-β-Catenin (Ser45) Antibody H, (M, R) W 9564 β-catenin Antibody H, M, R, Mk W, IP, IHC 9562 Phospho-GSK-3α/β (Ser21/9) Antibody H, M, R W, IHC 9331 Phospho-GSK-3β (Ser9) Antibody H, M, R, Mk W, IHC, IC 9336 GSK-3β Antibody H, M, R, X W, IHC, IC 9332 Phospho-CREB (Ser133) Antibody H, M, R, X W, IP, IHC, IC 9191 Phospho-CREB (Ser133) (87G3) Rabbit mab H, M, R W, IHC, F 9198 Phospho-CREB (Ser133) (1B6) Mouse mab H, M, R W 9196 CREB Antibody H, M, R, C W, IP, IHC, IC 9192 CREB (48H2) Rabbit mab H, M, R, Mk W, IP, IHC 9197 Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody H, M, R, Mk W, IP 4771 β-catenin Antibody #9562 and Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 488 Conjugate) #9708 Confocal immunofluorescence analysis of normal mouse colon, using β-catenin Antibody #9562 (red) and Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 488 Conjugate) #9708 (blue). Note specific colocalization in mitotic cell within the crypt. Application References Sinha, D. et al. (2005) Lithium activates the Wnt and phosphatidylinositol 3-kinase Akt signaling pathways to promote cell survival in the absence of soluble survival factors. Am. J. Physiol. Renal Physiol. 288, F703 F713. (#9561, #9336, #9332) Etheridge, S.L. et al. (2004) Expression profiling and functional analysis of Wnt signaling mechanisms in mesenchymal stem cells. Stem Cells 22, (#9561) Choi, J.H. et al. (2004) Augmentation of therapeutic angiogenesis using genetically modified endothelial progenitor cells with altered glycogen synthase kinase-3β activity. J. Biol. Chem. 279, (#9561, #9336) Kim, H.S. et al. (2002) Regulation of angiogenesis by glycogen synthase kinase-3β. J. Biol. Chem. 277, (#9561, #9336) Species Cross-Reactivity Key: H Human M Mouse R Rat Hm Hamster Mk Monkey C Chicken X Xenopus All All species expected ( ) 100% sequence homology Applications Key: W Western IP Immunoprecipitation IHC Immunohistochemistry IC Immunocytochemistry F Flow cytometry E ELISA D DELFIA

4 Akt Signaling Pathways Phospho-(Ser/Thr) Akt Substrate Antibody All W, IP, IHC, E, D 9611 Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mab All W, IP, IHC, E 9614 Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody All IP 9619 Phospho-Akt (Thr308) Antibody H, M, R, Hm, (C), X W, IP, IHC, F 9275 Phospho-Akt (Thr308) (244F9) Rabbit mab H, M, R W, IHC, F 4056 Phospho-Akt (Ser473) Antibody H, M, R, C, Hm W, IP, F 9271 Phospho-Akt (Ser473) Antibody (IHC Specific) H, M, R IHC, F 9277 Phospho-Akt (Ser473) (193H12) Rabbit mab H, M, R W, IP, F 4058 Phospho-Akt (Ser473) (736E11) Rabbit mab (IHC Specific) H, (M, R) IHC 3787 Phospho-Akt (Ser473) (587F11) Mouse mab H, M, R, Hm W, IP, IHC 4051 Akt Antibody H, M, R, C, Hm W, IP, F 9272 Immobilized Akt (1G1) Mouse mab H, M, R, Hm IP 9279 Akt (5G3) Mouse mab H, M, R, Hm IP, IC 2966 SignalSilence Akt sirna Kit H, Hm W 6210 Akt1 (2H10) Mouse mab H, M, R W, IP, IHC, F 2967 PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Kit H, M, R E 7160 PathScan Total Akt1 Sandwich ELISA Kit H, M E 7170 SignalSilence Akt1 sirna Kit (Human Specific) H W 6220 Akt2 Antibody H, M, R W, IP 2962 Akt2 (5B5) Rabbit mab H, M, R, Mk W, IP 2964 Akt2 (54G8) Rabbit mab (IHC Specific) H, (M, R) IHC 4057 SignalSilence Akt2 sirna Kit H W 6395 Akt3 Antibody H, M, R W, IP, IHC 4059 Akt3 (62A8) Rabbit mab H, M, R W, IP, IHC 3788 Phospho-(Tyr) p85 PI3 Kinase Binding Motif Antibody All W, IP, E, D 3821 PI3 Kinase p85 Antibody H, M, R W 4292 PI3 Kinase p110 α Antibody H, M, R W, IHC 4254 PI3 Kinase p110 γ Antibody H, M W, IP 4252 Phospho-PTEN (Ser380) Antibody H, M, R W, IP, IHC, IC 9551 Phospho-PTEN (Ser380/Thr382/383) Antibody H, M, R W, IP, IC 9554 PTEN Antibody H, M, R, Hm, Mk W, IP 9552 PTEN (138G6) Rabbit mab H, M, R, Mk W, IHC, F 9559 PTEN (26H9) Mouse mab H, M, R, Hm, Mk W, IP 9556 SignalSilence PTEN sirna Kit (Human Specific) H W 6250 Phospho-p70 S6 Kinase (Ser371) Antibody H, M, R W 9208 Phospho-p70 S6 Kinase (Thr389) Antibody H, M, R W 9205 Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mab H, M, R, Mk W, IHC, IC 9206 Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody H, M R W, IP, IHC 9204 p70 S6 Kinase Antibody H, M, R, Mk W, IP, IC, F 9202 p70 S6 Kinase (49D7) Rabbit mab H, M, R, Mk W, IHC 2708 Phospho-PTEN (Ser380) Antibody #9551 Confocal immunofluorescence analysis of normal mouse colon, using Phospho-PTEN (Ser380) Antibody #9551 (green). Note select cytoplasmic staining of a single presumptive stem cell.

5 Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mab #9614 Confocal immunofluorescence analysis of normal mouse colon, using Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mab #9614 (red). Note select staining of cells deep in the crypts. Application References: Sinor, A.D. and Lillien, L. (2004) Akt-1 expression level regulates CNS precursors. J. Neurosci. 24, (#9275, #9272) Paling, N.R. et al. (2004) Regulation of embryonic stem cell self-renewal by phosphoinositide 3-kinase-dependent signaling. J. Biol. Chem. 279, (#9611, #9271, #9272) Borowiak, M. et al. (2004) Met provides essential signals for liver regeneration. Proc. Natl. Acad. Sci. USA 101, (#9271, #9272) Jak/Stat Signaling GP130 Antibody H, M, R, Mk W 3732 Phospho-Jak1 (Tyr1022/1023) Antibody H, M W 3331 Jak1 Antibody H, M W, IP, IHC 3332 Phospho-Jak2 (Tyr221) Antibody H, M, (R) W 3774 Phospho-Jak2 (Tyr1007/1008) Antibody H, M, (R) W 3771 Phospho-Stat1 (Tyr701) Antibody H, M, R W, IHC, IC, F 9171 Phospho-Stat1 (Ser727) Antibody H, M, R W, IP, IC 9177 Stat1 (42H3) Rabbit mab (Human Specific) H, Mk W, IHC 9175 Stat1 Antibody H, M, R W, IP, F 9172 Stat1 (9H2) Mouse mab H, M W, IP, IHC, IC 9176 SignalSilence Stat1 sirna Kit H W 6330 Phospho-Stat3 (Tyr705) Antibody H, M, R, C W, IP, IHC, IC, F 9131 Phospho-Stat3 (Tyr705) (58E12) Rabbit mab H, M, (R) W, IP, IHC, IC 9135 Phospho-Stat3 (Tyr705) (3E2) Mouse mab H, M, (R) W, IP, IHC, F 9138 Phospho-Stat3 (Ser727) Antibody H, M, R W, IHC, IC 9134 Phospho-Stat3 (Ser727) (6E4) Mouse mab H, M W, IP, IHC, IC 9136 Stat3 Antibody H, M, R W, IP, IHC, IC, F 9132 Stat3 (79D7) Rabbit mab H, M, R, Mk W, IP, IHC, F 4904 Stat3 (124H6) Mouse mab H, M, R, Mk W, IP, IHC, IC, F 9139 PathScan Phospho-Stat3 (Tyr705) Sandwich ELISA Kit H E 7300 Phospho-Stat5 (Tyr694) Antibody H, M, R W, IC, F 9351 Phospho-Stat5 (Tyr694) (14H2) Mouse mab H, (M, R) W, IP 9356 Stat5 Antibody H, M W, IP 9352 Phospho-Tyk2 (Tyr1054/1055) Antibody H, (M, R) W 9321 Application References: Lannutti, B.J. and Drachman, J.G. (2004) Lyn tyrosine kinase regulates thrombopoietin-induced proliferation of hematopoietic cell lines and primary megakaryocytic progenitors. Blood 103, (#9131, #9132, #9351, #9352) Hsieh, J. et al. (2004) Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells. Proc. Natl. Acad. Sci. USA 101, (#9131, #9132) Sato, N. et al. (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat. Med. 10, (#9131, #9132) Species Cross-Reactivity Key: H Human M Mouse R Rat Hm Hamster Mk Monkey C Chicken X Xenopus All All species expected ( ) 100% sequence homology Applications Key: W Western IP Immunoprecipitation IHC Immunohistochemistry IC Immunocytochemistry F Flow cytometry E ELISA D DELFIA

6 TGF-β/Smad Signaling Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody H, M, R, X W, IHC, IC, F 9511 Smad1 Antibody H, Mk W 9512 Phospho-Smad2 (Ser245/250/255) Antibody H, M, (R) W, IC 3104 Phospho-Smad2 (Ser465/467) Antibody H, M, (R, C) W, IHC, IC 3101 Smad2 (34G6) Mouse mab H, M, (R, C, X) W 3107 Phospho-Smad3 (Ser433/435)/Smad1(Ser463/465) Antibody H, R W, IP 9514 Smad4 Antibody H, M, R, Mk W 9515 Smad5 Antibody H, M, R, Mk W 9517 TGF-β Receptor I Antibody H, R W 3712 TGF-β Receptor II Antibody H, M, R W 2518 TGF-β Receptor III Antibody H, M, (R) W, IP 2519 Phospho-Smad1 (Ser463/465) /Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody #9511 Confocal immunofluorescence analysis of normal mouse colon, using Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody #9511 (red) and Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 488 Conjugate) #9708 (blue). Phospho-Smad colocalizes with phosphohistone H3 in presumptive stem cells. Application References: Xu, R.H. et al. (2005) Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat. Methods 2, (#9511, #9512) He, X.C. et al. (2004) BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-β-catenin signaling. Nat. Genet. 36, (#9511) Species Cross-Reactivity Key: H Human M Mouse R Rat Hm Hamster Mk Monkey C Chicken X Xenopus All All species expected ( ) 100% sequence homology Applications Key: W Western IP Immunoprecipitation IHC Immunohistochemistry IC Immunocytochemistry F Flow cytometry E ELISA D DELFIA

7 Apoptosis/Caspase Pathways Phospho-Bcl-2 (Thr56) Antibody (Human Specific) H W 2875 Phospho-Bcl-2 (Ser70) Antibody (Human Specific) H W, F 2871 Bcl-2 Antibody H, M, R W 2876 Bcl-2 Antibody (Human Specific) H W, IP 2872 SignalSilence Bcl-2 sirna Kit (Human Specific) H, (M, R) W 6440 Cleaved Caspase-3 (Asp175) Antibody H, M, R W, IHC, IC, F 9661 Cleaved Caspase-3 (Asp175) (5A1) Rabbit mab H, M, R W, IP, IHC, IC, F 9664 Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor 488 Conjugate) H IC, F 9669 Caspase-3 Antibody H, M, R W, IP, IHC, IC 9662 Caspase-3 (8G10) Rabbit mab H, M, R W, IP, IC 9665 Caspase-3 (3G2) Mouse mab H W 9668 Apoptosis Marker: SignalStain Cleaved Caspase-3 (Asp175) IHC Detection Kit H, M IHC 8120 PathScan Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit H E 7190 Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) H, M, R IHC, IC 9667 SignalSilence Caspase-3 sirna Kit H W 6465 Cleaved Notch 1 (Val1744) Antibody H, M, R, Mk W, IP, IHC, IC, E 2421 Survivin (6E4) Mouse mab H, Mk W 2802 SignalSilence Survivin sirna Kit H W 6350 Tyrosine Kinase Signaling Phospho-FGF Receptor (Tyr653/654) Antibody H, (M, R) W, IHC 3471 Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mab H, (M, R) W 3476 FGF Receptor 1 Antibody H, (M, R) W, IP 3472 Phospho-c-Kit (Tyr719) Antibody H, M W, IP, IHC 3391 c-kit Antibody H, M W, IP 3392 Non-phospho-Src (Tyr416) (7G9) Mouse mab H, M, (R, C) W, IP 2102 Non-phospho-Src (Tyr527) Antibody H, (M, R, C) W 2107 Phospho-Src (Tyr527) Antibody H, M, R W, IHC 2105 Phospho-Src Family (Tyr416) Antibody H, M, R, C, (X) W, IHC, IC 2101 Src Antibody H, M, R, Mk W, IP, IHC 2108 Src (36D10) Rabbit mab H, M, R, Hm, Mk W, IP, IHC, IC 2109 Phospho-Tie2 (Tyr992) Antibody H, (M) W 4221 Phospho-Tie2 (Ser1119) Antibody H W 4226 Tie2 (AB33) Mouse mab H W, IP 4224 Phospho-VEGF Receptor 2 (Tyr951) Antibody H, (M) W, IP 2471 Phospho-VEGF Receptor 2 (Tyr996) Antibody H, (M) W, IP 2474 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mab H, M W, IHC 2478 Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mab M, (H) W 2477 VEGF Receptor 2 (55B11) Rabbit mab H, M W, IP, IHC, IC 2479 PathScan Phospho-VEGF Receptor 2 (Tyr1175) Sandwich ELISA Kit H E 7335 PathScan Total VEGF Receptor 2 Sandwich ELISA Kit H E 7340 Phospho-Tyrosine Mouse mab (P-Tyr-100) All W, IP, IHC, E, D, F 9411 Phospho-Tyrosine Mouse mab (P-Tyr-100) (Alexa Fluor 488 Conjugate) H F 9414 Phospho-Tyrosine Mouse mab (P-Tyr-100) (Alexa Fluor 647 Conjugate) H F 9415 Phospho-Tyrosine Mouse mab (P-Tyr-100) (Biotinylated) All W IP 9417 Immobilized Phospho-Tyrosine Mouse mab (P-Tyr-100) All IP 9419 Application References: Lennartsson, J. et al. (2004) Synergistic growth of stem cell factor and granulocyte macrophage colony-stimulating factor involves kinase-dependent and independent contributions from c-kit. J. Biol. Chem. 279, (#3391) Anneren, C. et al. (2004) The Src family of tyrosine kinases is important for embryonic stem cell self-renewal. J. Biol. Chem. 279, (#2101)

8 MAPK Signaling Phospho-Histone H3 (Ser10) Antibody H, M, R, Mk W, IP, IHC, IC, F 9701 Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 488 Conjugate) H, M IC, F 9708 Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 647 Conjugate) H, M F 9716 Phospho-Histone H3 (Ser10) Antibody (Fluorescein Conjugate) H, M, R IC 9704 Phospho-Histone H3 (Ser10) (6G3) Mouse mab H, M, R W, IP, IC, F 9706 Histone H3 Antibody H, M, R, Mk W, IHC, IC 9715 Histone H3 (3H1) Rabbit mab H, M, Hm, Mk W 9717 Mitotic Marker: SignalStain Phospho-Histone H3 (Ser10) IHC Detection Kit H, M IHC 8130 Histone Deacetylase 1 (HDAC1) Antibody H, M, R, Mk W, IP, IHC, IC 2062 Histone Deacetylase 3 (HDAC3) Antibody H, M, R, Mk W, IHC, IC 2632 Histone Deacetylase 4 (HDAC4) Antibody H, M, R W 2072 Histone Deacetylase 5 (HDAC5) Antibody H, M, R, Mk W, IP, IHC, IC 2082 Histone Deacetylase 6 (HDAC6) Antibody H, M, R, Mk W, IHC, IC 2162 Histone Deacetylase 7 (HDAC7) Antibody H, M, R W, IP, IC 2882 Phospho-HSP27 (Ser15) Antibody H, Mk W 2404 Phospho-HSP27 (Ser78) Antibody H, Mk W, IHC 2405 PathScan Phospho-HSP27 (Ser78) Sandwich ELISA Kit H, Mk E 7290 PathScan Total HSP27 Sandwich ELISA Kit H, Mk E 7295 Phospho-HSP27 (Ser82) Antibody H, Mk W, IHC, IC 2401 HSP27 (G31) Mouse mab H, Mk W, IHC, IC 2402 SignalSilence HSP27 sirna Kit (Human Specific) H W 6355 PathScan Phospho-Histone H3 (Ser10) Sandwich ELISA Kit H, M E 7155 Phospho-MEK1 (Thr286) Antibody H, R, Mk, (M) W, IP, IC 9127 Phospho-MEK1 (Ser298) Antibody H, M, R, Mk W 9128 MEK1 Antibody H, M, R, Mk W 9124 SignalSilence MEK1 sirna Kit (Human Specific) H W 6425 Phospho-MEK1/2 (Ser217/221) Antibody H, M, R W, IP, IC, F 9121 Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit mab H, M, R W, IHC, F 2354 MEK1/2 Antibody H, M, R W, F 9122 MEK1/2 (47E6) Rabbit mab H, M, R, Mk W, IC, F 9126 MEK1/2 (L38C12) Mouse mab H, M, R, Mk W, IHC, F 4694 MEK2 Antibody H, M, R, Mk W, IP 9125 SignalSilence MEK2 sirna Kit (Human Specific) H W 6430 Phospho-p38 MAPK (Thr180/Tyr182) Antibody H, M, R W, IP, IC, F 9211 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mab H, M, R, Mk W, IP, IC, F 9215 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mab (IHC Preferred) H, M, R, Mk W, IP, IHC, IC 4631 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mab H, M, R W, IP, F 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mab (Alexa Fluor 488 Conjugate) H, M F 4551 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mab (Alexa Fluor 647 Conjugate) H, M F 4552 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mab H, M, R IP 9219 p38 MAPK Antibody H, M, R W, IHC, F 9212 p38 MAPK (5F11) Mouse mab H, M, R W, IP, F 9217 p38 MAPK (5F11) Mouse mab (Alexa Fluor 488 Conjugate) H F 4556 p38 MAPK (5F11) Mouse mab (Alexa Fluor 647 Conjugate) H F 4555 p38 MAPK α Antibody H, M, R, Mk W, IP, IHC 9218 p38 MAPK δ Antibody H, M, R, Mk W, IP 9214 PathScan Phospho-p38 MAPK α (Thr180/Tyr182) Sandwich ELISA Kit H, M, R E 7140 SignalSilence Pool p38 MAPK sirna Kit H, (M) W 6385 Phospho-p44/42 MAPK (Thr202/Tyr204) Antibody H, M, R,C, Hm W, IP, IC, F 9101 Immobilized Phospho-p44/42 MAPK (Thr202/Tyr204) Mouse mab H, M, R IP 9109 Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mab H, M, R W, IC, F 4377 Phospho-p44/42 MAPK (Thr202/Tyr204) (20G11) Rabbit mab (IHC Preferred) H, M, R W, IHC, IC 4376 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mab H, M, R, Hm W, IP, IC, F 9106 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mab (Alexa Fluor 488 Conjugate) H, M F 4374 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mab (Alexa Fluor 647 Conjugate) H, M F 4375 p44/42 MAPK Antibody H, M, R, Hm W, IHC, F 9102 p44/42 MAPK (137F5) Rabbit mab H, M, R, Mk W, IP, IHC, F 4695 p44/42 MAPK (L34F12) Mouse mab H, M, R, Mk W, IP, IHC, F 4696 Growth/Proliferation Marker: SignalStain Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit H IHC 8110 PathScan Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit H, M E 7315 PathScan Total p44/42 MAPK Sandwich ELISA Kit H E 7320 Phospho-p90RSK (Thr359/Ser363) Antibody H, M, R, Mk W, IP, IHC, IC 9344 Phospho-p90RSK (Ser380) Antibody H, M, R, Hm W, IP 9341 Phospho-p90RSK (Thr573) Antibody H, M, R, Hm, X, (C) W, IHC 9346

9 Phospho-Histone H3 (Ser10) Antibody #9701 IHC analysis of paraffin-embedded mouse colon, using Phospho-Histone H3 (Ser10) Antibody #9701 showing differential localization in the villi. Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit mab #2354 Confocal immunofluorescence analysis of normal mouse colon, using Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit mab #2354 (red) and Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 488 Conjugate) #9708 (green). Note select staining of cells in the crypt. Phospho-p90RSK (Thr359/Ser363) Antibody #9344 Confocal immunofluorescence analysis of normal mouse colon, using Phospho-p90RSK (Thr359/Ser363) Antibody #9344 (red) and Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor 647 Conjugate) #9708 (blue). Note colocalization in presumptive stem cells. Application References: Kayali, A.G. et al. (2005) Growth factor-induced signaling of the pancreatic epithelium. J. Endocrinol. 185, (#9211, #9212, #9101) Kale, V.P. and Vaidya, A.A. (2004) Molecular mechanisms behind the dose-dependent differential activation of MAPK pathways induced by transforming growth factor-β1 in hematopoietic cells. Stem Cells Dev. 13, (#9211, #9212, #9101, #9102) Lennartsson, J. et al. (2004) Synergistic growth of stem cell factor and granulocyte macrophage colony-stimulating factor involves kinase-dependent and independent contributions from c-kit. J. Biol. Chem. 279, (#9121, #9106) Horiuchi, H. et al. (2004) Chicken leukemia inhibitory factor maintains chicken embryonic stem cells in the undifferentiated state. J. Biol. Chem. 279, (#9121, #9122) Ying, Q.L. et al. (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115, (#9211, #9101) Yoo, J. et al. (2003) Transforming growth factor-β-induced apoptosis is mediated by Smad-dependent expression of GADD45b through p38 activation. J. Biol. Chem. 278, (#9211, #9212, #9101, #9102) Garrington, T.P. et al. (2000) MEKK2 gene disruption causes loss of cytokine production in response to IgE and c-kit ligand stimulation of ES cell-derived mast cells. EMBO J. 19, (#9211, #9101)

10 p53 Signaling Acetylated-p53 (Lys382) Antibody H W 2525 Phospho-p53 (Ser6) Antibody H, M, Mk, (Hm) W, IP, IC 9285 Phospho-p53 (Ser9) Antibody H, Mk W, IP, IC 9288 Phospho-p53 (Ser15) Antibody H, M, R, Mk W, IP, IHC, IC 9284 Phospho-p53 (Ser15) (16G8) Mouse mab H W, IC 9286 PathScan Phospho-p53 (Ser15) Sandwich ELISA Kit H E 7365 PathScan Total p53 Sandwich ELISA Kit H E 7370 Phospho-p53 (Thr18) Antibody H W 2529 Phospho-p53 (Ser20) Antibody H, M, Mk W, IHC, IC 9287 Phospho-p53 (Ser33) Antibody H, (Mk) W, IHC, IC 2526 Phospho-p53 (Ser37) Antibody H, Mk W, IP, IC 9289 Phospho-p53 (Ser46) Antibody H, Mk W, IP, IC 2521 Phospho-p53 (Thr81) Antibody H, Mk W, IHC, IC 2676 Phospho-p53 (Ser315) Antibody H, (Mk) W 2528 Phospho-p53 (Ser392) Antibody H, M, Mk W, IC 9281 p53 Antibody H, R, Mk W, IP, IC 9282 p53 (7F5) Rabbit mab H, Mk W, IHC 2527 p53 (1C12) Mouse mab H, M, R, Mk W, IP, IC 2524 SignalSilence p53 sirna Kit (Human Specific) H W 6230 Application References: Zhang, X. et al. (2005) The ATM/p53/p21 pathway influences cell fate decision between apoptosis and senescence in reoxygenated hematopoietic progenitor cells. J. Biol. Chem. 280, (#9286) Hong, Y. and Stambrook, P.J. (2004) Restoration of an absent G1 arrest and protection from apoptosis in embryonic stem cells after ionizing radiation. Proc. Natl. Acad. Sci. U.S.A. 101, (#9284, #9287) Other Signaling Proteins Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody H, M, R, Mk W, IHC, IC 3141 Ras Antibody H, M, R, Mk, (C, Hm, X) W 3965 R-Ras Antibody H, M, R, Mk W, IP 3962 Phospho-Ras-GRF1 (Ser916) Antibody M, R W 3321 Ras-GRF1 Antibody M W, IHC 3322 Phospho-cdk2 (Thr160) Antibody H, M, R W, IP, E, F 2561 Presenilin 1 Antibody H, R, Mk, (M) W, IP 3622 Presenilin 2 Antibody H, M, R, Mk W, IP, IHC, IC 2192 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141 Phospho-cdk2 (Thr160) Antibody #2561 IHC analysis of paraffin-embedded mouse colon, using Phospho-Ezrin (Thr567)/Radixin (Thr564) /Moesin (Thr558) Antibody #3141, showing differential localization in the crypt. IHC analysis of paraffin-embedded mouse colon, using Phospho-cdk2 (Thr160) Antibody #2561, showing differential localization in the crypt. Species Cross-Reactivity Key: H Human M Mouse R Rat Hm Hamster Mk Monkey C Chicken X Xenopus All All species expected ( ) 100% sequence homology Applications Key: W Western IP Immunoprecipitation IHC Immunohistochemistry IC Immunocytochemistry F Flow cytometry E ELISA D DELFIA

11 Visit our Website The preferred source of innovative tools & information for signal transduction research with over 450 activation-state antibodies, 80 recombinant kinases and 25 ELISA kits. Browse Products by Pathway Our browse products by pathway feature immediately locates and delivers relevant search results with just one click of your mouse. In addition to our extensive print catalog, we are also constantly improving and updating our website. Search capability and site navigation have been improved, and online ordering of our products is now available. We provide our customers with upto-the-minute information on new products. Our website also serves as a valuable technical resource for researchers working in the field of signal transduction. Browse Products by Pathway Pathway Diagrams, Protein Kinases and Protein Domains New Drug Discovery Website New Kinase-Disease Association Table Enhanced e-commerce Capabilities Choose any Product Pathway from our menu, roll your cursor over the pathway to highlight the protein of interest, click and all relevant products are immediately displayed. Rabbit Monoclonal Antibody produced using technology from Epitomics, Inc. under U.S. Patent No. 5,675,063. INNOVATIVE DISCOVERY TOOLS FOR SIGNAL TRANSDUCTION RESEARCH Dr./Mr./Ms. Institution/Company Department PO Box Address City/State Zip Phone I m on your mailing list, but my address has changed (please include previous affiliation): Please send me The Cell Signaling Technology Catalog Product updates by CST does not distribute or exchange mailing lists. Orders (toll free) CELL (2355) / orders@cellsignal.com Fax ordering Human Kinome poster The New England Biolabs Catalog Technical support (toll-free) TECH (8324) / support@cellsignal.com Keep me informed about product developments regarding: Target and Drug Discovery Chromatin Regulation/Acetylation MAP Kinase Signaling Apoptosis/Caspase Signaling Akt Signaling Pathways Translational Control PKC and Phospholipase Signaling Cell Cycle/Checkpoint Control Cytokine Signaling Neuroscience Tyrosine Kinases/Docking Proteins Cytoskeletal Regulation Glucose/Energy Metabolism Lymphocyte Signaling WNT/ Nuclear Signaling Other IMMUNOHISTOCHEMISTRY FLOW CYTOMETRY HIGH THROUGHPUT SCREENING Printed in the USA on 100% recycled (50% post-consumer) waste fiber using soy inks and processed chlorine free. STEMCELL05

12 Wnt Akt Jak/Stat TGF-β MAPK p53 Our New Catalog & Technical Reference. NEW PRODUCTS: Over 50 Rabbit Monoclonal Antibodies Over 100 new Active Kinases Over 100 new Activation-State- Specific Antibodies Fluorescent Conjugated Antibodies for Flow and Image Cytometry NEW KITS: PhosphoScan (P-Tyr-100) Kit Pathscan Sandwich ELISA Kits Kinase Assay and Substrate Screening Kits IHC Detection Kits Antibody Sampler Kits NEW REFERENCE MATERIAL: New and Updated Pathway Diagrams Protein Kinase Dendrograms and Domain Diagrams New Kinase-Disease Association Table IHC and Flow Cytometry Protocols