Microbiological water testing

Transcription

1 Microbiological water testing New edition! More information! New products!

2 Scharlau microbiology History Scharlau Chemie S.A. has been manufacturing laboratory chemicals since In 1979 ADSA-MICRO started the manufacture of dehydrated media. This company was acquired by Scharlau Chemie S.A. in 1992 and the brand changed to Scharlau. A new facility for the manufacture of dehydrated media was set up in 2002, which allowed us to increase capacity and to further improve the quality of our media. ISO 9001:2000 quality assurance was put in place since ISO 14001:1996 for environmental care was obtained in In 2002 Scharlau Chemie S.A. started offering also a wide range of prepared media and supplements. Microbiological culture media Scharlau manufactures two separate lines of culture media, dehydrated and prepared media; also stains in solution, reagents, supplements and rapid confirmation tests (see more information at the end of this leaflet). Please ask for the specific catalogues and brochures. NEW Certificates New certificate including picture of typical growth. Additives and supplements in vial 1 2 Prepared culture media New sterilized presentation: fast, easy and less risk of contamination. Click, shake and dispense. Selection of the most common media in different presentations. Dehydrated culture media Scharlau Chemie S.A. manufactures dehydrated culture media for microbiology. Available in 100 g and 500 g bottles Each bottle comes in an individual bag to avoid dust and a COA (certificate of analysis) is included with each bottle Description in 5 languages Tamper-proof cap No inside aluminium foil Perfectly tight-closing cap insures long shelf life NEW Packaging PAC-O-VAC is the novel airtight packing from Scharlau that provides an extraordinary protection to microbiology culture media. Vacuum, followed by replacement of residual air with nitrogen, guarantees that our products are protected from contamination by moisture, dust particles or microorganisms. This assures optimum storage conditions and prevent the products from altering their organoleptic or physical properties. 90 mm Petri plates. Available also with single and double flow pack bag and gamma irradiated for use in clean room. 55 mm filtration plates. Prepared plates of solid medium for the membrane filtration technique. Packed in blister. Rodac plates for surfaces control. Packaging into blisters inside clean room to avoid contamination and dehydration. Agars in flasks. Long shelf life in different volumes for remelting. Broths in flasks. Enrichment media and diluents. Sterility control. Agars in tubes for remelting. For mass inoculation technique. Broths in tubes. Differents volumes depending on working protocol. Index Escherichia coli

3 Prepared solid media used for water analysis Filtration plates Prepared 55 mm filtration plates in blister packs Each blister pack comes in a sealed flowpack bag. This kind of packaging increases the shelf life to over 6 months, avoiding fast dehydration and contamination. The latest directives, relative to the analysis of water for human consumption, indicate the use of media with agar. Such media allow more precise enumeration, since without an excess of water, the colonies can grow perfectly separated and with the typical morphology. Formulations follow specifications of the various Pharmacopoeiae and ISO regulations. Packaging into blisters inside the clean room to avoid contaminations. Advantages Shelf life of 6 months Storage at room temperature Individual packing Double wrap Empty blister works as work tray Bubble-free surface that allows perfect contact with the filtration membrane Blister packaging guarantees very slow dehydration of the medium Thanks to a minimum of 10 ml of medium per plate, correct results can be obtained even after long incubation times Additional cellophane bag Conditioning The plates are packed in a blister with 6 compartments. Each compartment carries 1 plate. So every time you open a compartment, you use 1 plate. In a box you will find 5 blisters with a total of 30 plates. Each blister is covered with 1 additional cellophane bag. Prepared liquid media in 2 ml plastic ampoules Liquid media continue to be useful in the microbiological analysis of water (not for human consumption), beverages and liquids in general. Our presentation in 2 ml mono-dose plastic ampoules with a shelf life of over 12 months allows its use with any kind of membrane that follows the relevant directive. Our design allows even distribution on the filter pad. Plastic ampoules of 2 ml mono-dose Advantages Long shelf life Even distribution on filter pad One ampoule per membrane Tel Fax

4 Water analysis according to EN-ISO directives Membrane filtration technique The water sample is passed through a 0,45 μm membrane filter. Microorganisms are retained on the surface of the membrane. Membrane with microorganisms is placed on a Petri dish of 55 mm that contains the appropriate agar. After incubation at right temperature and time, colonies that have grown on the membrane surface can be directly counted. This MF technique offers a series of advantages over the traditional MPN technique (Most Probable Number): Much more representative sampling. The membrane allows reco very of very low concentration of microorganims in large volumes of liquid. Therefore this technique is suitable for very low contaminated water. Definitive results in less time. More precise and direct enumeration of the colonies. Indicated for strongly mineralized waters that give wrong reactions in the colimetry in liquid media. Membranes can be kept for a long period of time for testimonial, medical or legal purposes. Considerable savings in space, labour and laboratory material as compared to the MPN technique. This technique has been adopted also for the control of water not destined to human consumption, e.g. process water in manufacturing. Also used for microbiological control of other liquid samples like juices, milk, etc. Analytical funnels, WHATMAN 100 ml Ready-to-use fiiltration units for microbiology with removable membrane. After filration, the membrane of the analytical funnel can be used for a wide range of biological analyses. Save time with no flaming and sterlization required. Minimizes the risk of cross contamination. Easy release of membrane. Individually packaged. Cat. no. Description Pore size (μm) (mm) Units White/Balck Grid 0, White/Balck Grid 0, Balck Grid/White 0, Microbiological filtration system MBS I, WHATMAN Membranes for microbiological control Made from cellulose nitrate developed for microbiological control Sterile, individually packed Pore size 0,45 μm With a high contrast grid (3,1 mm) Cat. no. Description Pore (μm) Color/Grid (mm) Units ES ,45 White/Black Scharlau Microplus-21 STL 0,45 White/Black 47 4x100 Whatman Microplus-21 ST 0,45 White/Black 47 1x100 Whatman Microplus-21 STL 0,45 White/Black 47 4x100 Whatman Microplus-21 ST 0,45 White/Black 50 1x100 Whatman Microplus-21 STL 0,45 White/Black 47 4x100 Whatman 1 Special MicroPlus membrane filters developed for microbiological quality control with high mechanical strength and high flow rate. Ideal for samples with a high particle content and viscous samples. 2 MicroPlus type STL in dispenser boxes with 100 numbered filters for easy removal and safe handling with the Membrane Dispenser. Electrical membrane filter dispenser for microbiological control, WHATMAN The membrane dispenser (Membrane-Butler E) offers optimal handling for MicroPlus STL membrane filters. The dispenser bos is placed in a Membrane-Butler, the sterile packaging is inserted into the roller system and the system is ready. Pressing the push button or the foot pedal a membrane filter is ejected from sterile packing and can be removed easily with forceps. Filtration system for optimal microbiological control using membranes. The overwall procedure time is reduced to a minimum. The special sealiling tecnhnique guarantees easy handling and a good integrity of the funnle and membrane during filtration. This reduces any cross contamination to a minimum. Simple to tuse Save sealing mechanism Shorter preparation time High reproducibilty Less waste an better economny done to 50 autoclave cycles Easier to validate Risk of cross confirmation is minimized The design o f the system, consist of: An electrical membrane dispenser A funnel dispenser A vacuum Cat. no. Description Units place Vacuum Filtration Manifold A Dispenser for funnels for 100 ml and 350 ml ml plastic funnel of PP (autoclavable) ml plastic funnel of ABS (autoclavable) ml plastic funnel of PP (autoclavable) Autoclaving bags for funnels Dispenser for funnels for 100 ml and 350 ml 1 Cat. no. Description Manual dispenser (Membrane-Buttler) Whatman Electrical dispenser (Membrane-Buttler-E) Whatman Tower for two Buttler-E Whatman Foot pedal for automatic dispensing Whatman

5 Water sampling Sampling Sampling has to be done with sterile containers. Picture 1 A B C J Option 1: are borosilicate glass or non toxic plastic (PP) bottles that are heat resistant. Option 2: are non toxic sterile plastic bags of single use. Option 3: are non toxic plastic bottles (PE) that are not heat resistant, but have been sterilized by gamma irradiation. Chlorinated water samples should be collected in a bottle that contains sodium thiosulphate. Ionized square PE bottles Transluced polyethylene (PE). Good visibility Excellent shock resistance Excellent tightness Cat. no. Size (ml) Mouth (mm) Cap Units 064-BA0000 (Picture 1) J 25 Scharlau 630FC A 100 P. Gosselin 630LC A 175 P. Gosselin 064BA00001 (Picture 1) J 15 Scharlau 00LC A 90 P. Gosselin Bottles for chlorinated samples with sodium thiosulphate (20 mg/l*) Cat. no. Size (ml) Mouth (mm) Cap Units 064-BA2140 (Picture 1) J 25 Scharlau 00HLC C 140 P. Gosselin 064BA21401 (Picture 1) J 15 Scharlau 0HLC102001* C 85 P. Gosselin HFC B 84 P. Gosselin *Special for Legionella, according ISO Whirl-Pak bags with sodium thiosulfate For chlorinated water sampling. 300 ml of capacity (10 mg). Whirl-Pak a sampling big overture that facilitates samples. The sodium thiosulfate inhibits the effects of chlorine, bromine and ozone present in the water when the sample is taken. Little volume comparing with usual bottles. Tight closure, white label to identify sample. Cat. no. Cap. (ml) Dimensions (mm) Units x230 With long of sodium thiosulfate 100 Coliforms and Escherichia coli NF EN ISO :2000 Chapman TTC Agar (Tergitol 7 Agar) Enterococci NF EN ISO :2001 Slanetz - Bartley Agar Enumeration of culturable microorganisms NF EN ISO 6222:1999 Tryptone Yeast Extract Agar Clostridium perfringens (inclusive of spores) EUROPEAN DIRECTIVE 98/83/CE m-cp Agar Tests will be performed by the membrane filtration method Tel Fax

6 Water analysis according to EN-ISO directives Coliforms and Escherichia coli Chapman TTC Agar (Tergitol 7 Agar) (Lactose TTC Sodium Heptadecylsulfate Agar) Intended use Selective medium for the detection and enumeration of coliforms, heat-tolerant coliforms and Escherichia coli following the membrane filtration method. This medium is mentioned in the EN ISO :2000 directives. Comments Tergitol 7 inhibits the growth of Gram positive bacteria. TTC solution added to the medium serves as bacterial growth indicator and for an earlier recognition of Escherichia coli and Enterobacter aerogenes. Instructions Filtrate 100 ml of sample water through membrane of pore size 0,45μm. Use sterile forceps to carefully place the membrane, filtered side up, on top of the agar of the filtration plate. E. coli Incubation Incubate for (21 ± 3) hours at (36 ± 2) C and avoid excessive dehydration of the plate. Interpretation Colonies capable of fermenting the lactose and the consequent production of acid that leads to a colour change of the medium from green to yellow are to be considered suspicious. Supplements TTC Other bacteria Confirmation All suspicious colonies have to be confirmed with Indole and Oxidase reagents. Count as coliforms all characteristic colonies that are oxidase-negative. Colonies with oxidase-negative and indole-positive reations should be considered E. coli. Ordering information Chapman TTC Agar Base * * Chapman TTC Agar 064-PA PF0003 TTC Sterile Solution 1% *Needs adition of TTC

7 Water analysis according to EN-ISO directives Enterococci Slanetz & Bartley Agar Intended use Selective medium for enumeration and detection of Enterococci in drinking water, waste water and beverages following the membrane filtration method. According to EN ISO :2000 directives. Comments The medium contains sodium azide to inhibit the growth of Gram negative bacteria. TTC solution added to the medium allows better counting. Instructions Filtrate 100 ml of sample water through membrane of pore size 0,45 micron. Use forceps to carefully place the membrane, filtered side up, on top of the agar of the filtration plate. Slanetz-Bartley no growth Enterococcus sp. Incubation Incubate for (44 ± 4) hours at (36 ± 2) C and avoid excessive dehydration of the plate. Interpretation Colonies capable of growing in presence of sodium azide and of reducing TTC to form red, brown or pink colour. Confirmation Carry out confirmation of suspicious colonies using Bile Esculin Azide Agar (01-592) observing those colonies that are capable of hydrolizing the esculin within one to two hours forming colours that may range from brown to black. Supplements TTC Ordering information Slanetz-Bartley Agar Base * * TTC Sterile Solution 1% Slanetz-Bartley Agar ** ** 064-PA PF0004 *Needs adition of TTC **TTC is included in formulation Tel Fax

8 Water analysis according to EN-ISO directives Enumeration of culturable microorganisms Tryptone Yeast Extract Agar Intended use Solid medium for the enumeration of culturable microorganisms by colony count in water. According to EN ISO 6222:1999. Over 300 colonies Comments The nutrients of the Tryptone and the vitamins of the yeast extract favour the growth of most bacteria. Instructions Mass inoculation technique. 2 sets each of sterile Petri dishes. In series 1 we will place 1 ml of water. In series 2 we will place 0,1 ml of water. 10 to 15 ml of medium, once melted and kept at (45 ± 1) C, must be poured over the samples avoiding bubbles colonies Incubation One set of inoculated plates at (36 ± 2) C for (44 ± 4) hours and the other set at (22 ± 2) C for (68 ± 4) hours. Interpretation All colonies are counted, expressing the result in amount of colonies per ml. Only plates containing colonies are used for enumeration. If there are over 300 colonies per ml, test will have to be repeated with higher dilutions. Ordering information Tryptone Yeast Extrract Agar Base Tryptone Yeast Extract Agar 064-TA PF0013* *30 plates

9 Water analysis according to EN-ISO directives Clostridium perfringens (inclusive of spores) m-cp Agar Intended use Solid medium for enumeration and isolation of Clostridium perfringens in water for human consumption by membrane filtration method.according to European Directive 98/83/CE. Comments Clostridia hydrolyse the saccharose forming yellow colonies. After exposure to ammonia vapours, the colonies change to red colour. Instructions Filtrate 100 ml of sample water through membrane of pore size 0,45 micron. Use forceps to carefully place the membrane, filtered side up, on top of the agar of the filtration plate. Incubation Incubate under anaerobic conditions for (21 ± 3) hours at (44 ± 1) C. Clostridium perfringens before development Interpretation Colonies of opaque yellow colour are to be considered suspicious. Confirmation Plate has to be submitted to the effects of vapours of ammonia for seconds. Those colonies that change from yellow to pink or red will be counted. Filtration plate support, ref Q7080 Clostridium perfringens after development m-cp developer: Ammonia, solution 25% r.g., AM0257 Supplements Selective and chromogenic mixture, ref CASE. Ordering information Development process Filtration plate support, ref Q7080. m-cp Agar Base * * m-cp Agar m-cp Selective Supplement Ammonia solution *Needs adition of supplement **12 plates AM PF0030C**/064-PF CASE Tel Fax

10 Water analysis according to EN-ISO directives Filtrate Sodium Thiosulphate Transport filters to plates Chapman TTC Agar (Tergitol 7 Agar) (36 ± 2) C - (21 ± 3) H. Slanetz-Bartley Agar (36 ± 2) C - (44 ± 4) H. m-cp Agar (44 ± 1) C - (21 ± 3) H. Anaerobic Oxidase + -- Discard Indole + -- E. coli Discard Black colony = Enterococcus sp. BEA (44 ± 0,5) C - 2 H. Colony without change = Discard Change to red = Clostridium perfringens Ammonia No change = Discard

11 Dehydrated and prepared media used for water analysis (2) (2) (1) Detection of total and fecal coliforms Chapman TTC Agar (Tergitol 7 Agar) ISO PA PF TA PF0003V E. coli Direct Agar (ECD Agar) Endo Agar Base (4) (4) Endo LES Agar Base (4) (4) Endo MF Broth Base (4) (4) Fecal Coliforms Agar (FC Agar, m-fc) (6) (6) 064-PF PF0024V Lauryl Sulphate Broth ISO 064-TA0451 (3) Tryptone Bile Agar ISO PF0014 Detection of fecal enterococci Bile Esculin Azide Agar ISO / FDA PF0008 Kenner Fecal Agar (KF Agar) (4) (4) Kenner Fecal Broth (KF Broth) (4) (4) Slanetz-Bartley Agar ISO (5) (5) 064-PA PF PF0004V Slanetz-Bartley Agar Base ISO (4) (4) Total enumeration of aerobic microorganisms m-hpc (Heterotrophic plate count) Nutrient Agar ISO / FDA PF PF0001V Plate Count Agar (PCA) ISO / FDA/ USP PA PF PF0010V R2A EP PA0072I 064-PF0072 Tryptic Soy Agar (TSA) EP / USP PA PA0031I 064-PF0026 Tryptone Yeast Extract Agar ISO PF0013 Detection of Clostridia m-cp (Clostridium Perfringens) Agar Base CE Directive PF0030/064PF0030C (7) Iron Sulphite Agar SPS Agar (Clostridium Selective Agar) PA PF PF0002V TSC Agar (8) (8) 064-TA6021 (9) Detection of Pseudomonas Cetrimide Agar (Pseudomonas Selective Agar) EP / USP PA PF0005V CN Selective Agar Base for Pseudomonas ISO PF0005 Detection of Staphylococci Mannitol Salt Agar (Chapman Agar) FDA / USP PA PF PF0025V Detection of moulds and yeasts m-green Agar 064-PF0089 Sabouraud Chloramphenicol Agar PA PA0026I 064-PF0022 Sabouraud Dextrose Agar PA PA0025I 064-PF0006 Detection of Vibrio TCBS Medium PA plates 90 mm 20 plates 90 mm 30 plates 55 mm 20 tubes 20 vials (1) Liquid media in 2 ml vials for use with pads (2) Ask for availability of prepared 90 mm plates in your country (3) 9 ml tubes with Durham tubes (4) Needs addition of CASE (5) Needs addition of CASE (6) Needs addition of CASE (7) 12 plates 55 mm (8) Needs addition of CASE (9) 9 ml tubes with paraffin Tel Fax

12 Chromogenic Media Chromogenic media offer distinct advantages. Reliable detection in less time Simple simultaneous isolation and differentiation of pathogens thanks to the discernible colours No need to perform the complex and expensive detection protocols aswith traditional media Well defined colours of the colonies avoiding colour diffusion of traditional media Faster results allow quicker release of manufactured products Short analytical times mean lower labour costs Chromogenic detection leads to reliable results No need to change standard working procedures Chromogenic Media Scharlau Colinstant Chromogenic Medium Intended use Chromogenic selective and differential medium for direct identification of Escherichia coli and coliforms present in water and food. Suitable for membrane filtration method. Principle Based on the detection of two enzyme activities: ß-D-Glucuronidase (ß gluc) ß-D-Galactosidase (ß gal) Improved formula with better inhibition of the Gram positive accompanying flora, using bile salts instead of Tergitol. After incubation at 37 C for 24 hours, E. coli colonies appear in blue colour and the balance coliforms in salmon colour. The remaining enterobacteria do no present any coloration. E. coli Coliforms: Citrobacter freundii Coliforms: Klebsiella pneumoniae Tryptone bile -glucuronide Agar (TBX) Intended use Chromogenic selective and differential medium for enumeration and direct detection of E. coli ß-glucuronidase positive in water and food. According ISO and Can be used mass inoculation method or membrane filtration method. Principle Inhibition of accompanying flora using bile salts and high temperature incubation. After incubation at 44 C for hours, E. coli colonies ß-glucuronidase positives appear in green-blue colour. Fast: TBX: Direct identification and enumeration of Escherichia coli in 24 hours. Direct identification and enumeration of E. coli and coliforms in 24 hours. Easy to use: Easy dissolution Ready-to-use: Medium requires no addition Easy interpretation: Better colour contrast Other Enterobacteria: Salmonella enterica Other Enterobacteria X-Glu (+): Shigella sonnei Ordering information Colinstant Chromogenic Medium PA PF6015 TBX Agar BA PA0945 Chromogenic Media CHROMagar Scharlab S.L. distributes now the CHROMagar Chromogenic Media. These are the media used in water testing. Coliforms and E. coli E. coli bacteria is a fecal contamination indicator. Traditional methods for detecting E. coli are extremely tedious and usually require a heavy workload since many suspect colonies have to be tested. CHROMagar E. coli. Is a culture medium which directly marks E. coli colonies in blue colour EC EC169 5 litres* 25 litres* CHROMagar ECC. Will additionally show the other coliforms as red colonies. This is another useful indicator of questionable hygiene conditions EF EF323 5 litres* 25 litres*

13 Chromogenic Media Chromogenic Media CHROMagar CHROMagar Liquid ECC. Is used as a broth for the pad technique for the detection of E. coli and coliforms in water samples. The inoculated filtration membrane is put on top of a pad presoaked with CHROMagar Liquid ECC EL382 5 litres* CHROMagar Vibrio. V. parahaemolyticus, V. vulnificus & V. cholerae are pathogenic bacteria which can cause serious seafood poisoning. For the detection of those bacteria, traditional methods (TCBS) are time consuming, require heavy workload and are not very sensitive. On the contrary, CHROMagar Vibrio medium helps to easily differentiate V. parahaemolyticus, V. vulnificus & V. cholerae, from other Vibrio directly during the isolation step by colony colour with a sensitivity higher than conventional methods VB912 5 litres* CHROMagar Pseudomonas. For the simultaneous detection and enumeration of Pseudomonas aeruginosa with markedly different colouring (blue colonies) PS822 5 litres* Presence/Absence test Coliforms Presence/Absence Broth Chromogenic Medium Intended use Direct and easy detection of presence or absence of total coliforms and of Escherichia coli in water samples. Comments Chromogenic and fluorogenic broth that combines the test of the enzymes ß-D-Glucuronidase and ß-D-Galactosidase and the indole test* in a single flask, ready for use. No growth Positive growth for coliforms Instructions Add 100 ml of sample water to the flask that contains the presence/absence medium. Incubation Incubate sample for hours at C. E. coli Confirmation Total coliforms will change the medium to green colour. Escherichia coli will emit fluorescence under UV light and be positive with indole test*. *To perform Indole Test, please add 2-3 ml of Kovacs Reagent (Cat. no ). Ordering Information Chromogenic P/A Broth 064-BACOLI Kovacs Indole Reagent Tel Fax

14 Diagnostic tests Oxidase Test For detection of cytochrome oxidase in oxidase producing microorganisms like Pseudomonas sp. Sterile swabs drenched with Oxidase reagent, ready for use. Oxidase test positive Description Cat. no. Presentation Oxidase test sterile swabs units Reagent in powder form. 1% aqueous solution has to be prepared for consumption within one week. Storage at 4 C protected from light. Description Cat. no. Presentation Oxidase reagent in powder g ONPG + ONPG - E. coli Coliforms ONPG-FDA-MUG-INDOLE Test Selective broth for the simultaneous detection of total coliforms and E. coli in water and food. This detection has been improved with a new mixture of chromogenic substrates. Presence of coliforms gives a blue-greenish colour, and presence of E. coli also gives fluorescence under UV light; and it can also been confirmed with the adition of Kovacs reagent (indole test). Description Cat. no. Presentation ONPG-FDA-MUG-INDOLE Test 064-TA tubes of 2 ml Indole Test Indole Tryptone Water Tryptone water, specially supplemented with tryptophan for Indole tests. Kovacs Indole Reagent Intended use Classical test to differentiate Escherichia and Enterobacteria. Also used for the differentiation of other non enteric microorganisms. Comments Many microorganisms can produce Indole from Tryptophan thanks to a Tryptophanase, in a process favoured by oxygen and inhibited by glucose. Media used in this test should contain no glucose, should present a high Tryptophan content and should be incubated aerobically. Instructions The pure culture to be verified has to be inoculated in the medium. Weak positive Negative Positive Incubation Incubate sample for hours at 35 C. This incubation can be reduced to 4 hours, if you part from a massive inoculum in solid medium and if you inoculate in a small volume of culture medium (Indole Test Cat. no. 064-TA0132). Confirmation After incubation you proceed to the identification with Kovacs Indole Reagent. Ordering information Description Cat. no. Presentation Kovacs Indole Reagent ml flask Kovacs Indole Reagent ml flask Tryptone Water-Indole Test 064-TA tubes of 2 ml Tryptone Water 064-TA tubes of 9 ml

15 Diagnostic tests Bile Esculin Azide Agar Solid medium for the enterococci confirmative test in water by membrane filtration method. According to EN ISO :2000. Ordering information Bile Esculin Azide Agar Bile Esculin Azide Agar PF0008 Other related products 1 Petri Plates, PLASTIQUES GOSSELIN All plates are ISO 5 (Class100) aseptic or esterile R. 2 Cat. no. (mm) Alture Description Presentation Units NA (Picture 1) 90 14,2 Aseptic, 3 vents Bags of 33 units NE (Picture 1) 90 14,2 Sterile, 3 vents Bags of 33 units A (Picture 2) 55 14,2 Aseptic, 3 vents Bags of 15 units E (Picture 2) 55 14,2 Sterile, 3 vents Bags of 15 units 1620 With vents: Specially designed to ensure that cultures are well ventilated. Petri Plates with sterile pads, WHATMAN Cat. no. (mm) Units Anaerobic jarr, SCHARLAU For incubation in anaerobic conditions. Cat. no. Volume (l) Capacity 064-AG0R25 2,5 12 plates Tel Fax

16 Technical documentation Culture Media Handbook Edition no. 10 The latest edition no. 10 is ready. Over 250 pages with helpful information and photos of our media. Spiral bound for easy and space-saving manipulation on the work bench. International edition written in English language. Cross reference charts available separately in Excel format. New MSDS can be downloaded from our website: Contains over 350 different products. Microbiology catalogues 1 2 NEW CAT-WATER9 (7-2009) 8 9 NEW 10 NEW 11 NEW 1: Dehydrated culture media handbook 2: NEW Culture media for microbiology catalogue 3: Microbiological water testing leaflet 4: Clinical microbiology leaflet 5: Food analysis leaflet 6: RPF leaflet 7: CHROMagar leaflet 8: Colinstant leaflet 9: NEW Easy kits for sampling surfaces 10: NEW Cryoinstant 11: NEW Selective supplements for culture media leaflet Scharlab S.L. Tel Fax