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1 Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Supporting Information Synthesis and optical properties of covalently bound Nile Red in mesoporous silica hybrids - Comparison of dye distribution of materials prepared by facile grafting and by co-condensation routes. Markus Börgardts, a Kathrin Verlinden, b Manuel Neidhardt, c Tobias Wöhrle, c Annika Herbst, b Sabine Laschat, c Christoph Janiak, b and Thomas J.J. Müller*,a a b c Institut für Organische Chemie und Makromolekulare Chemie, Heinrich-Heine- Universität Düsseldorf, Universitätsstraße 1, Düsseldorf, Germany. Institut für Anorganische Chemie und Strukturchemie, Abteilung für Metallorganische Chemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, Düsseldorf, Germany. Institut für Organische Chemie, Universität Stuttgart, Pfaffenwaldring 55, Stuttgart, Germany. Table of Contents 1. Structural characterization of grafted and cocondensed materials 6 and Nitrogen sorption measurements Small angle x-ray scattering (SAXS) Transmission electron microscopy (TEM) Spectroscopic properties of precursor 5, and hybrid materials 6 and Solvatochromism of precursor Determination of dye concentration inside materials 6 and Solvatochromism of hybrid materials 6 and Excitation and emission spectra of hybrid materials 6 and 7 in the solid state Red-edge excitation shift (REES) of hybrid materials 6b and 7e Fluorescence quenching of hybrid materials 6b and 7e... 24
2 1. Structural characterization of grafted and cocondensed materials 6 and Nitrogen sorption measurements volume / cm³g ,0 relative pressure / p/p 0 6a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 1: Nitrogen sorption isotherms of grafted samples 6. The deep adsorption increase of hybrid 6h at relative pressures close to the saturation pressure is attributed to nitrogen condensation in interparticle voids volume / cm³g ,0 relative pressure / p/p 0 7a 7b 7c 7d 7e 7f 7g 7h Figure S 2: Nitrogen sorption isotherms of co-condensed samples 7.
3 dv(r) / cm³g -1 nm -1 0,14 0,13 0,12 0,11 0, ,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5 5,0 pore diameter / nm 6a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 3: pore size distribution curves of grafted samples 6. 0,18 0,16 0,14 dv(r) / cm³g -1 nm -1 0,12 0, ,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5 5,0 pore diameter / nm 7a 7b 7c 7d 7e 7f 7g 7h Figure S 4: pore size distribution curves of co-condensed samples 7.
4 1.2. Small angle x-ray scattering (SAXS) i 6h 6g log intensity / a.u. 6f 6e 6d 6c 6b 6a / Figure S 5: SAXS pattern of grafted samples 6.
5 h 7g 7f log intensity / a.u. 7e 7d 7c 7b 7a / Figure S 6: SAXS pattern of co-condensed samples 7.
6 1.3. Transmission electron microscopy (TEM) Figure S 7: TEM images of 6a a) along the channel direction and b) perpendicular to the channel direction. Figure S 8: : TEM images of 6b a) along the channel direction and b) perpendicular to the channel direction.
7 Figure S 9: TEM images of 6c a) along the channel direction, b) perpendicular to the channel direction and c) large scale. Figure S 10: TEM images of 6d a) along the channel direction and b) perpendicular to the channel direction.
8 Figure S 11: TEM images of 6e a) along the channel direction and b) perpendicular to the channel direction. Figure S 12: TEM images of 6f a) along the channel direction and b) perpendicular to the channel direction.
9 Figure S 13: TEM images of 6g a) along the channel direction, b) perpendicular to the channel direction and c) mixed phase. Figure S 14: TEM images of 6h a) along the channel direction and b) perpendicular to the channel direction.
10 Figure S 15: TEM images of 6i a) along the channel direction and b) perpendicular to the channel direction. Figure S 16: TEM images of 7a a) along the channel direction and b) perpendicular to the channel direction.
11 Figure S 17: TEM images of 7b a) along the channel direction and b) perpendicular to the channel direction. Figure S 18: TEM images of 7c a) along the channel direction and b) perpendicular to the channel direction.
12 Figure S 19: TEM images of 7d a) along the channel direction and b) perpendicular to the channel direction. Figure S 20: TEM images of 7e a) along the channel direction and b) perpendicular to the channel direction.
13 Figure S 21: TEM images of 7f a) along the channel direction and b) perpendicular to the channel direction. Figure S 22: TEM images of 7g a) along the channel direction and b) perpendicular to the channel direction.
14 Figure S 23: TEM images of 7h a) along the channel direction and b) perpendicular to the channel direction.
15 2. Spectroscopic properties of precursor 5, and hybrid materials 6 and Solvatochromism of precursor 5 1,0 normalized absorption Hexane CHCl 3 1,4-Dioxane ipr 2 O DCM Et 2 O DCE THF EtOH EtOAc MeOH DMSO MeCN Acetone Figure S 24: Absorption spectra of 5 in different solvents. 1,0 normalized emission Hexane CHCl 3 1,4-Dioxane ipr 2 O DCM Et 2 O DCE THF EtOH EtOAc MeOH DMSO MeCN Acetone Figure S 25: Emission spectra of 5 in different solvents. The molar extinction coefficient of precursor 5 in DMSO was determined to be 539nm = M -1 cm -1.
16 2.2. Determination of dye concentration inside materials 6 and 7 0,14 0,12 0,10 absorption a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 26: UV/Vis-spectra of grafted hybrid materials suspended in DMSO with c(6a) = 3.0 g/l, c(6b-6g) = 0.3 g/l, c(6h) = 0.15 g/l, c(6i) = 0.12 g/l. 0,12 0,10 8 absorption a 7b 7c 7d 7e 7f 7h 7g Figure S 27: UV/Vis-spectra of co-condensed hybrid materials suspended in DMSO with c(7a) = 10 g/l, c(7b - 7e) = 3.0 g/l, c(7f) = 0.30 g/l, c(7g) = g/l, c(7h) = g/l.
17 2.3. Solvatochromism of hybrid materials 6 and 7 1,0 normalized emission Et 2 O 1,4-Dioxane Hexane H 2 O Figure S 28: normalized emission spectra of 6b in different solvents. 1,0 normalized emission intensity Et 2 O 1,4-Dioxane Hexane H 2 O Figure S 29: normalized excitation spectra of 6b in different solvents ( emission = 660 nm).
18 1,0 normalized emission Hexane CHCl 3 1,4-Dioxane ipr 2 O DCM Et 2 O DCE THF EtOH EtOAc MeOH H 2 O DMSO MeCN Acetone Figure S 30: normalized emission spectra of 7e in different solvents. 1,0 normalized intensity Hexane CHCl 3 1,4-Dioxane ipr 2 O DCM Et 2 O DCE THF EtOH EtOAc MeOH H 2 O DMSO MeCN Acetone Figure S 31: normalized excitation spectra of 7e in different solvents ( emission = 650 nm).
19 2.4. Excitation and emission spectra of hybrid materials 6 and 7 in the solid state 1,0 wavenumber / cm ,0 normalized intensity normalized intensity a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 32: normalized excitation spectra of grafted hybrid materials 6 ( emission = 700 nm). wavenumber / cm intensity b 6c 6d 6e 6f 6h 6i intensity Figure S 33: excitation spectra of grafted hybrid materials 6 ( emission = 700 nm). (Hybrid materials 6a and 6g not shown as they were measured in another setup.)
20 1,0 wavenumber / cm ,0 normalized intensity normalized intensity a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 34: normalized emission spectra of grafted hybrid materials fluorecence intensity fluorecence intensity a 6b 6c 6d 6e 6f 6g 6h 6i Figure S 35: emission spectra of grafted hybrid materials 6.
21 1,0 wavenumber / cm ,0 normalized intensity normalized intensity a 7b 7c 7d 7e 7f 7g 7h Figure S 36: normalized excitation spectra of co-condensed hybrid materials 7 ( emission = 700 nm). wavenumber / cm intensity intensity a 7b 7c 7d 7e 7f 7g 7h Figure S 37: excitation spectra of co-condensed hybrid materials 7 ( emission = 700 nm).
22 1,0 wavenumber / cm ,0 normalized intensity normalized intensity a 7b 7c 7d 7e 7f 7g 7h Figure S 38: normalized emission spectra of co-condensed hybrid materials wavenumber / cm fluorescence intensity fluorescence intensity a 7b 7c 7d 7e 7f 7g 7h Figure S 39: emission spectra of co-condensed hybrid materials 7.
23 2.5. Red-edge excitation shift (REES) of hybrid materials 6b and 7e 1,0 normalized intensity nm 500 nm 520 nm 530 nm 547 nm 570 nm 580 nm 590 nm 600 nm 610 nm 620 nm 630 nm 640 nm 650 nm Figure S 40: Fluorescence spectra of 6b at designated excitation wavelengths showing REES. 1,0 normalized intensity nm 500 nm 520 nm 541 nm 560 nm 570 nm 580 nm 590 nm 600 nm 610 nm 620 nm Figure S 41: Fluorescence spectra of 7e at designated excitation wavelengths showing REES.
24 2.6. Fluorescence quenching of hybrid materials 6b and 7e fluorescence intensity c(hcl) / mmoll Figure S 42: fluorescence quenching of 6b in water upon addition of designated amounts of HCl. fluorescence intensity c(hcl) / mmoll Figure S 43: excitation spectra of fluorescence quenching of 6b in water upon addition of designated amounts of HCl ( emission = 720 nm).
25 fluorescence c(hcl) / mmoll Figure S 44: fluorescence quenching of 7e in water upon addition of designated amounts of HCl. excitation intensity c(hcl) / mmoll Figure S 45: excitation spectra of fluorescence quenching of 7e in water upon addition of designated amounts of HCl ( emission = 720 nm).
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