Food and Environmental Analysis. A Quicker. and Easier. Sample Preparation Choice.

Size: px

Start display at page:

Download "Food and Environmental Analysis. A Quicker. and Easier. Sample Preparation Choice."

Hester Gregory
5 years ago
Views:

1 A Quicker and Easier Sample Preparation Choice Food and Environmental Analysis /roq

2 QuEChERS 1 What is QuEChERS? QuEChERS is a Sample Preparation Technique for: Complex sample matrices Wide range of compounds (typically pesticides) Food and environmental samples What does it stand for? Quick Easy Cheap Effective Rugged Safe (QuEChERS) 3 How does it help me? QuEChERS is a simplified step sample preparation process that: Significantly reduces sample preparation time Is simple, inexpensive, and easy to use And is effective and rugged at extracting and cleaning samples for a wide range of compounds For more details visit /roq If roq QuEChERS Kits do not perform as well or better than your current QuEChERS product, return the product with comparative data within 45 days for a FULL REFUND. 16 Phenomenex, Inc. All rights reserved.

Table of Contents How Does roq QuEChERS Work?...p. 4 Choose Your roq QuEChERS Kits...p. 5 Fast and Easy Food Applications...pp. 6-9 Reliable Environmental Applications...pp. 1-11 Ordering Information.

3 Table of Contents How Does roq QuEChERS Work?...p. 4 Choose Your roq QuEChERS Kits...p. 5 Fast and Easy Food Applications...pp. 6-9 Reliable Environmental Applications...pp Ordering Information...pp roq QuEChERS...p. 1 HPLC/UHPLC Columns...p. 13 roq QuEChERS Resources and Tools...p. 14 GC Column Selection and Resources...p. 15 Phenomenex l WEB: 3

How Does roq QuEChERS Work? STEP 1 Extraction* Easily Weigh Out Samples Original Packet hodsulfate Met g Magnesium 4. Chloride 1.

g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe x.com Originald Packet Methoum Sulfate mene pheno www. 4. g Magnesi Chloride 1.

c henom www.p Originald Packet thoium Sulfate Meg Magnes 4. Chloride 1. g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe x.com mene Blend fruits or vegetables to be analyzed.

4 How Does roq QuEChERS Work? STEP 1 Extraction* Easily Weigh Out Samples Original Packet hodsulfate Met g Magnesium 4. Chloride 1. g Sodium No Mess Salt Packets p/n: KS-891 AH-94 & Tube Packet p/n: Packet ex.com enomen Originald Packet thoium Sulfate Meg Magnes 4. Chloride 1. g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe x.com Originald Packet Methoum Sulfate mene pheno www. 4. g Magnesi Chloride 1. g Sodium Originald Packet Methoum Sulfate 1 p/n: KS-89 AH-94 & Tube Packet p/n: Packet 4. g Magnesi Chloride 1. g Sodium & Tube Packet p/n: Packet 1 p/n: KS-89 AH-94 om enex.c henom om enex.c henom Originald Packet thoium Sulfate Meg Magnes 4. Chloride 1. g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe x.com mene Blend fruits or vegetables to be analyzed. Weigh blended sample. Add salts and acetonitrile. Shake tube for 1 minute. Centrifuge tube for 5 minutes. Original Packet hodm Sulfate Met g Magnesiu 4. Chloride 1. g Sodium 1 p/n: KS-89 AH-94 & Tube Packet p/n: Packet STEP om enex.c henom Clean Up/dSPE** Avoid Leaky Tubes Our tubes are designed to seal completely Originald Packet thoium Sulfate Meg Magnes Originald Packet thoium Sulfate Meg Magnes 4. Chloride 1. g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe 4. Chloride 1. g Sodium 91 p/n: KS-8 4 AH-9 t & Tube Packe t p/n: Packe x.com mene Add supernatant from extraction procedure into a roq dspe tube. Shake dspe tube for 3 seconds. STEP 3 Analysis by GC or HPLC Centrifuge dspe tube for 5 minutes. Cleaner Extracts.8e5.6e5.4e5.e5.e5 1.8e5 1.6e5 1.4e5 1.e5 1.e5 8.e4 6.e4 4.e4.e4 roq kits contain low extractable centrifuge tubes reducing background noise in chromatograms App ID 759 pheno www. x.com mene pheno www. Intensity, cps pheno www Salts and Sorbents used in roq Kits Extraction: Magnesium Sulfate(MgSO4) Sodium Acetate(NaOAc) Sodium Chloride(NaCI) Sodium Citrate Tribasic Dihydrate(SCTD) Sodium Citrate Dibasic Sesquihydrate(SCDS) 4 Phenomenex l WEB: Clean Up/dSPE: Magnesium Sulfate (MgSO4) Primary/Secondary Amine (PSA) Endcapped C18 Sorbent (C18E) Graphitized Carbon Black (GCB) min

Choose Your Kit STEP 1 Extraction * AOAC AOAC 7.1 Method 6. g MgSO 4,1.5 g NaOAc KS-8911 STEP ORIGINAL Non-Buffered Method 4. g MgSO 4, 1. g NaCl KS-891 6. g MgSO 4, 1.5 g NaCl KS-891 EN EN 1566 Method 4.

1 EN 1566 1 ml 8 ml 1 ml 6 ml General 15 mg MgSO 4 5 mg PSA KS-89 1 mg MgSO 4 4 mg PSA KS-898 15 mg MgSO 4 5 mg PSA KS-8916 9 mg MgSO 4 15 mg PSA KS-894 Fats and Waxes 15 mg MgSO 4 5 mg PSA 5 mg C18E

5 Choose Your Kit STEP 1 Extraction * AOAC AOAC 7.1 Method 6. g MgSO 4,1.5 g NaOAc KS-8911 STEP ORIGINAL Non-Buffered Method 4. g MgSO 4, 1. g NaCl KS g MgSO 4, 1.5 g NaCl KS-891 EN EN 1566 Method 4. g MgSO 4, 1. g NaCl, 1. g SCTD,.5 g SCDS KS-899 Clean Up/dSPE ** AOAC 7.1 EN ml 8 ml 1 ml 6 ml General 15 mg MgSO 4 5 mg PSA KS-89 1 mg MgSO 4 4 mg PSA KS mg MgSO 4 5 mg PSA KS mg MgSO 4 15 mg PSA KS-894 Fats and Waxes 15 mg MgSO 4 5 mg PSA 5 mg C18E KS mg MgSO 4 4 mg PSA 4 mg C18E KS mg MgSO 4 5 mg PSA 5 mg C18E KS mg MgSO 4 15 mg PSA 15 mg C18E KS-891 Pigmented 15 mg MgSO 4 5 mg PSA 5 mg GCB KS mg MgSO 4 4 mg PSA 4 mg GCB KS mg MgSO 4 5 mg PSA.5 mg GCB KS mg MgSO 4 15 mg PSA 15 mg GCB KS-89 Highly Pigmented 15 mg MgSO 4 5 mg PSA 7.5 mg GCB KS mg MgSO 4 15 mg PSA 45 mg GCB KS-893 Pigments and Fats 15 mg MgSO 4 5 mg PSA 5 mg GCB 5 mg C18E KS mg MgSO 4 4 mg PSA 4 mg GCB 4 mg C18E KS-895 GC LC Core-Shell Technology GC Columns *All roq Extraction kits contain fifty easy-pour salt packets and fifty 5 ml stand-alone centrifuge tubes. **All roq dspe kits contain pre-weighed sorbents/salts inside ml or 15 ml centrifuge tubes. 5

6 Food Applications Multi-Residue Pesticides Screening: Fruits and Vegetables Easily Prepare Samples with roq QuEChERS Kits STEP 1 STEP Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Extraction EN 1566 Method 4. g MgSO 4, 1. g NaCl, 1. g SCTD,.5 g SCDS KS-899 Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Fully Porous LC/MS/MS Option Clean up/dspe EN 1566 Method 15 ml dspe Kits 9 mg MgSO 4, 15 mg PSA KS-894 Column: Synergi.5 µm Fusion-RP 1 Å Dimensions: 5 x. mm Part No.: B-443-B SecurityGuard : AJ-486 Mobile Phase: A: Water/Methanol (9:1) + 5 mm Ammonium formate B: Methanol/Water (9:1) + 5 mm Ammonium formate Gradient: Time (min) % B 1 1 Flow Rate: 4 µl/min Temperature: 4 C Detection: Tandem Mass Spectrometer (MS/MS) (4 C) Detector: SCIEX API 3 System Sample: Pesticide mix 1.5e6 1.e6 Intensity, cps 9.5e5 9.e5 8.5e5 8.e5 7.5e5 7.e5 6.5e5 6.e5 5.5e5 5.e5 4.5e5 4.e5 3.5e5 3.e5.5e5.e5 1.5e5 1.e5 5.e4 SCREEN FOR 535 PESTICIDES IN UNDER 18 MINUTES Full Range Selectivity LC Columns App ID min 6

Food Applications STEP 1 STEP Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Extraction EN 1566 Method 4. g MgSO4, 1. g NaCl, 1.

7 Food Applications STEP 1 STEP Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Extraction EN 1566 Method 4. g MgSO4, 1. g NaCl, 1. g SCTD,.5 g SCDS KS-899 Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Clean up/dspe EN 1566 Method 15 ml dspe Kits 9 mg MgSO4, 15 mg PSA KS-894 Column: Kinetex 5 µm Biphenyl 1 Å Dimensions: 1 x.1 mm Part No.: D-467-AN Mobile Phase: A: 5 mm Ammonium formate in Water B: 5 mm Ammonium formate in Methanol Gradient: Time (min) % B Flow Rate:.5 ml/min Temperature: 35 C Detection: Tandem Mass Spectrometer (MS/MS) ( C) Detector: SCIEX 45 QTRAP Sample: 175+ Pesticide mix Core-Shell LC/MS/MS Option 5.4e4 5.e4 4.5e4 Core-Shell Technology 4.e4 3.5e4 Intensity, cps 3.e4.5e4.e4 ENHANCED POLAR PESTICIDES RETENTION 1.5e4 1.e min App ID 618 7

Food Applications Mycotoxins Screening: Grains Easily Prepare Samples with roq QuEChERS Kits STEP 1 STEP Analytical Column: Kinetex Core-Shell.6 µm Biphenyl 1.7e6 Original Method Packet 4.

g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Clean up/dspe EN 1566 Method 15 ml dspe Kits 9 mg MgSO4, 15 mg PSA KS-894 Column: Kinetex.

8 Food Applications Mycotoxins Screening: Grains Easily Prepare Samples with roq QuEChERS Kits STEP 1 STEP Analytical Column: Kinetex Core-Shell.6 µm Biphenyl 1.7e6 Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Extraction EN 1566 Method 4. g MgSO 4, 1. g NaCl, 1. g SCTD,.5 g SCDS KS-899 Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Clean up/dspe EN 1566 Method 15 ml dspe Kits 9 mg MgSO4, 15 mg PSA KS-894 Column: Kinetex.6 µm Biphenyl Dimensions: 5 x.1 mm Part No.: B-46-AN Mobile Phase: A: 5 mm Ammonium acetate with.1% Acetic acid B: Methanol with 5 mm Ammonium acetate with.1% Acetic acid Gradient: Time (min) % B Flow Rate:.45 ml/min Temperature: Ambient Detection: MS/MS (SCIEX API 4 ) Sample: Acetyldeoxynivalenol. DAS 3. FB1 4. HT Toxin 5. FB 6. T Toxin 7. Aflatoxin M1 8. Aflatoxin G 9. Ochratoxin A 1. Aflatoxin G1 11. Aflatoxin B 1. Aflatoxin B1 1.5e6 ENHANCED RETENTION AND SENSITIVITY 1 Core-Shell Technology Intensity, cps 1.e ,9 5.e5. 7 5, min App ID

Food Applications Antibiotics Analysis: Meat and Animal Tissues Easily Prepare Samples with roq QuEChERS Kits STEP 1 STEP Original Method Packet 4. g Magnesium Sulfate 1.

g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Clean up/dspe 15 ml dspe Kits 9 mg MgSO 4, 15 mg PSA, 15 mg C18E KS-891 Column: Kinetex.6 µm Biphenyl 1 Å Dimensions: 5 x.1 mm Part No.

9 Food Applications Antibiotics Analysis: Meat and Animal Tissues Easily Prepare Samples with roq QuEChERS Kits STEP 1 STEP Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Extraction AOAC 7.1 Method 6. g MgSO 4, 1.5 g NaOAc KS-8911 Original Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-94 Clean up/dspe 15 ml dspe Kits 9 mg MgSO 4, 15 mg PSA, 15 mg C18E KS-891 Column: Kinetex.6 µm Biphenyl 1 Å Dimensions: 5 x.1 mm Part No.: B-46-AN Mobile Phase: A: Water with.1 % Formic Acid B: Methanol with.1 % Formic Acid Gradient: Time (min) 5 7 % B 1 1 Flow Rate:.5 ml/min Temperature: Ambient Detection: Tandem Mass Spectrometer (MS/MS) ( C) Detector: SCIEX 45 QTRAP Sample: 41 Antibiotics Analytical Column: Kinetex Core-Shell.6 µm Biphenyl.1e6.e6 1.8e6 Core-Shell Technology 1.6e6 1.4e6 Intensity, cps 1.e6 1.e6 8.e5 6.e5 4.e5.e min App ID 161 9

10 HPLC Environmental Products Applications (Fully Porous) Hormones: Marine & Fresh Water Sediment Modified roq QuEChERS Protocol STEP 1 STEP Extraction 1. Weigh g of dry sediment into a 5 ml centrifuge. Spike sample with internal standards 3. Add 1 ml of reagent water and mix 4. Add 1 ml of acidified acetonitrile (.1% acetic acid) and shake for 1 seconds 5. Add sodium acetate (1.5 g) and MgSO 4 (. g), or weigh out 3.5 g of AOAC 7.1 roq extraction packet AH Shake for 1 seconds and vortex for 1 minute 7. Centrifuge at 4 rpm for 5 min 8. Cool sample at ºC for 1.5 hours or until frozen Clean-up 1. Take 8-9 ml of the supernatant from the previous step and transfer to a 15 ml dspe tube (KS-896). Shake for 1 seconds and vortex for 1 minute 3. Centrifuge for 1 minutes at 3 rpm 4. Aspirate 5 ml of supernatant and filter using a Phenex. µm PTFE filter (AF--1) into a test tube suitable for a dry-down station 5. Evaporate sample using a dry-down station at 35 ºC to near dryness 6. Reconstitute by first adding 5 µl of acetone, vortexing, and then adding 95 µl of methanol/water (1:1) 7. Transfer the reconstituted sample to an autosampler vial for analysis Column: Kinetex.6 μm XB-C18 Dimensions: 5 x.1 mm Part No.: B-46-AN Mobile Phase: A: Water B: Acetonitrile Gradient: Time (min) % B Injection: 1 µl Flow Rate:.5 ml/min Temperature: 3 C Detector: SCIEX 55 QTRAP Detection: MS/MS (ESI + and ESI -) Sample: 1. Estriol (E3). 17-b Estradiol (E) 3. Testosterone (TEST) 4. Equilin (EQ) α-Ethnylestradiol (EE) 6. Estrone (E1) 7. Androstenedione (AND) Note: Post column infusion with.5 % NH 4 OH Analytical Column: Kinetex.6 µm XB-C ENHANCED RETENTION AND SENSITIVITY Core-Shell Technology 3 Intensity, cps min App ID

11 HPLC Products Environmental (Core-Shell) Applications Pyrethroids: Marine & Fresh Water Sediment Modified roq QuEChERS Protocol STEP 1 STEP Extraction 1. Weigh g of dry sediment into a 5 ml centrifuge. Spike sample with internal standards 3. Add 1 ml of reagent water and mix 4. Add 1 ml of acidified acetonitrile (.1% acetic acid) and shake for 1 seconds 5. Add sodium acetate (1.5 g) and MgSO 4 (. g), or weigh out 3.5 g of AOAC 7.1 roq extraction packet AH Shake for 1 seconds and vortex for 1 minute 7. Centrifuge at 4 rpm for 5 min 8. Cool sample at ºC for 1.5 hours or until frozen Clean-up 1. Take 8-9 ml of the supernatant from the previous step and transfer to a 15 ml dspe tube (KS-896). Shake for 1 seconds and vortex for 1 minute 3. Centrifuge for 1 minutes at 3 rpm 4. Aspirate 5 ml of supernatant and filter using a Phenex. µm PTFE filter (AF--1) into a test tube suitable for a dry-down station 5. Evaporate sample using a dry-down station at 35 ºC to near dryness 6. Reconstitute by first adding 5 µl of acetone, vortexing, and then adding 95 µl of methanol/water (1:1) 7. Transfer the reconstituted sample to an autosampler vial for analysis Column: Kinetex.6 μm EVO C18 Dimensions: 5 x.1 mm Part No.: B-475-AN Mobile Phase: A: 5mM Ammonium acetate B: Methanol Gradient: Time (min) 1 3 % B Injection: 5 µl/min Flow Rate:.7 ml/min Temperature: C Detector: SCIEX Triple Quad 45 System Detection: MS/MS Sample: 1. Cyfluthrin. Cyhalothrin 3. Cypermethrin 4. Delamethrin 5. Esfenvalerate 6. cis-permethrin 7. trans-permethrin 8. Bifenthrin Analytical Column: Kinetex.6 µm EVO C18 Intensity, cps 4.8e4 4.6e4 4.4e4 4.e4 4.e4 3.8e4 3.6e4 3.4e4 3.e4 3.e4.8e4.6e4.4e4.e4.e4 1.8e4 1.6e4 1.4e4 1.e4 1.e ENHANCED RETENTION AND SENSITIVITY 1 1 3,4, Core-Shell Technology 3 min App ID

12 Ordering Information roq Extraction Kits Extraction kits contain fifty easy-pour salt packets and fifty 5 ml stand-alone centrifuge tubes Description Unit Part No. AOAC 7.1 Method Extraction Kits 6. g MgSO 4, 1.5 g NaOAc 5/pk KS-8911* EN 1566 Method Extraction Kits 4. g MgSO 4, 1. g NaCl, 1. g SCTD,.5 g SCDS 5/pk KS-899* Original Non-buffered Method Extraction Kits 4. g MgSO 4, 1. g NaCl 5/pk KS g MgSO 4, 1.5 g NaCl 5/pk KS-891 *AOAC and EN Extraction Kits also available in traditional non-collared 5 ml centrifuge tubes, Part No.: KS-8911-NC and KS-899-NC roq dspe Kits dspe kits contain pre-weighed sorbents/salts inside ml or 15 ml centrifuge tubes Bulk roq QuEChERS Sorbents Phase 1 g 1 g C18-E 4G-4348 GCB (Graphitized Carbon Black) 4D G-4615 PSA 4G-461 roq Extraction Salt Packets Salt packets only. Centrifuge tubes not included. Description Unit Part No. AOAC 7.1 Method Extraction Packets 6. g MgSO 4, 1.5 g NaOAc 5/pk AH-943 EN 1566 Method Extraction Packets 4. g MgSO 4, 1. g NaCl, 1. g SCTD,.5 g SCDS 5/pk AH-941 Original Non-Buffered Method Extraction Packets 4. g MgSO 4, 1. g NaCl 5/pk AH g MgSO 4, 1.5 g NaCl 5/pk AH-944 Description Unit Part No. ml dspe Kits 15 mg MgSO 4, 5 mg PSA, 5 mg C18E 1/pk KS mg MgSO 4, 5 mg PSA,.5 mg GCB 1/pk KS mg, MgSO 4, 5 mg PSA, 7.5 mg GCB 1/pk KS mg MgSO 4, 5 mg PSA 1/pk KS mg MgSO 4, 5 mg PSA, 5 mg C18E, 5 mg GCB 1/pk KS mg MgSO 4, 5 mg PSA, 5 mg C18E 1/pk KS mg MgSO 4, 5 mg PSA, 5 mg GCB 1/pk KS mg MgSO 4, 5 mg PSA 1/pk KS ml dspe Kits 9 mg MgSO 4, 15 mg PSA, 15 mg C18E 5/pk KS mg MgSO 4, 15 mg PSA, 15 mg GCB 5/pk KS-89 9 mg MgSO 4, 15 mg PSA, 45 mg GCB 5/pk KS mg MgSO 4, 15 mg PSA 5/pk KS mg MgSO 4, 4 mg PSA, 4 mg C18E, 4 mg GCB 5/pk KS mg MgSO 4, 4 mg PSA, 4 mg C18E 5/pk KS mg MgSO 4, 4 mg PSA, 4 mg GCB 5/pk KS mg MgSO 4, 4 mg PSA 5/pk KS-898 If roq QuEChERS Kits do not perform as well or better than your current QuEChERS product, return product with comparative data within 45 days for a FULL REFUND. 1

13 LC Ordering Information Synergi Whether your pesticides are hydrophobic or polar, Synergi Fusion-RP columns provide you with a full range of selectivity ensuring separation of the most challenging and complex mixtures. Synergi.5 µm High Speed Technology (HST) Columns (mm) Phases 3 x. 5 x. 1 x. 5 x 3. 1 x 3. 5 x 4.6 Fusion-RP A-443-B B-443-B D-443-B B-443-Y B-443-Y B-443-E SecurityGuard Synergi 4 µm Microbore and Minibore Columns (mm) Phases 5 x x 1. 3 x. 5 x. 75 x. 15 x. 5 x. 4 x.* 1/pk Fusion-RP B-444-A F-444-A A-444-B B-444-B C-444-B F-444-B G-444-B AJ-7556 for ID:.-3. mm SecurityGuard Synergi 4 µm MidBore Columns (mm) Phases 5 x x 3. 5 x 3. 4 x.* 1/pk Fusion-RP B-444-Y F-444-Y G-444-Y AJ-7557 for ID:.-3. mm SecurityGuard Synergi 4 µm Analytical Columns (mm) Phases 5 x x x x x.* 1/pk Fusion-RP B-444-E C-444-E F-444-E G-444-E AJ-7557 for ID: mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-48 Learn more about Synergi columns at /synergi If Synergi analytical columns do not provide at least an equivalent separation as compared to a competing column of similar particle size, similar phase and dimensions, send in your comparative data within 45 days and for a FULL REFUND. Kinetex Get the most out of every HPLC/UHPLC analysis. Kinetex Core-Shell Technology produces increased efficiencies over traditional fully porous columns, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity. Kinetex 5 µm Columns (mm) SecurityGuard ULTRA SecurityGuard ULTRA SecurityGuard ULTRA Phases 5 x.1 3/pk 5 x 3. 3/pk 5 x x x x 4.6 3/pk EVO C18 B-4633-AN AJ-998 B-4633-Y AJ-997 B-4633-E D-4633-E F-4633-E G-4633-E AJ-996 Biphenyl B-467-AN AJ-99 B-467-Y AJ-98 B-467-E D-467-E F-467-E G-467-E AJ-97 XB-C18 B-465-AN AJ-878 B-465-Y AJ-8775 B-465-E D-465-E F-465-E G-465-E AJ-8768 for.1 mm ID for 3. mm ID for 4.6 mm ID Kinetex.6 µm Columns (mm) SecurityGuard ULTRA SecurityGuard ULTRA SecurityGuard ULTRA Phases 5 x.1 15 x.1 3/pk 5 x 3. 3/pk 5 x x x 4.6 3/pk EVO C18 B-475-AN F-475-AN AJ-998 B-475-Y AJ-997 B-475-E D-475-E F-475-E AJ-996 Biphenyl B-46-AN F-46-AN AJ-99 B-46-Y AJ-98 B-46-E D-46-E F-46-E AJ-97 XB-C18 B-4496-AN F-4496-AN AJ-878 B-4496-Y AJ-8775 B-4496-E D-4496-E F-4496-E AJ-8768 for.1 mm ID for 3. mm ID for 4.6 mm ID SecurityGuard ULTRA Kinetex 1.7 µm Columns (mm) Phases 5 x.1 1 x.1 15 x.1 3/pk EVO C18 B-476-AN D-476-AN F-476-AN AJ-998 Biphenyl B-468-AN D-468-AN F-468-AN AJ-99 XB-C18 B-4498-AN D-4498-AN AJ-878 for.1 mm ID *SecurityGuard ULTRA cartridges require holder, Part No.: AJ-9. For additional Kinetex dimensions and phases, please visit /kinetex If you are not completely satisfied with Kinetex core-shell columns, return th column with comparative data within 45 days for a FULL REFUND. 13

An aliquot of the organic layer from the extraction step is further cleaned up by dispersive SPE. This step helps selectively remove unwanted interferences such as lipids and pigments.

14 Pesticides and analytes of interest are first extracted from the sample. The process relies on the combination for organic solvent and various salts to partition the analytes from samples into an organic layer. An aliquot of the organic layer from the extraction step is further cleaned up by dispersive SPE. This step helps selectively remove unwanted interferences such as lipids and pigments. Extracted and cleaned sample is injected into a gas or liquid chromatography system for analysis..8e5.6e5.4e5.e5.e5 1.8e5 1.6e5 1.4e5 1.e5 1.e5 8.e4 6.e4 4.e4.e4 Cleaner Extracts roq kits contain low extractable centrifuge tubes reducing background noise in chromatograms min AOAC 7.1 Method 6. g MgSO4,1.5 g NaOAc General Fats and Waxes Pigmented Higly Pigmented Pigments and Fats Non-Buffered Method 4. g MgSO4, 1. g NaCl 6. g MgSO4, 1.5 g NaCl EN 1566 Method 4. g MgSO4, 1. g NaCl, 1. g SCTD,.5 g SCDS AOAC 7.1 EN ml 8 ml 1 ml 6 ml 15 mg MgSO4 1 mg MgSO4 15 mg MgSO4 9 mg MgSO4 5 mg PSA 4 mg PSA 5 mg PSA 15 mg PSA P/N KS-89 P/N KS-898 P/N KS-8916 P/N KS mg MgSO4 1 mg MgSO4 15 mg MgSO4 9 mg MgSO4 5 mg PSA 4 mg PSA 5 mg PSA 15 mg PSA 5 mg C18E 4 mg C18E 5 mg C18E 15 mg C18E P/N KS-8918 P/N KS-896 P/N KS-8913 P/N KS mg MgSO4 1 mg MgSO4 15 mg MgSO4 9 mg MgSO4 5 mg PSA 4 mg PSA 5 mg PSA 15 mg PSA 5 mg GCB 4 mg GCB.5 mg GCB 15 mg GCB P/N KS-8919 P/N KS-897 P/N KS-8914 P/N KS mg MgSO4 9 mg MgSO4 5 mg PSA 15 mg PSA 7.5 mg GCB 45 mg GCB P/N KS-8915 P/N KS mg MgSO4 1 mg MgSO4 5 mg PSA 4 mg PSA 5 mg GCB 4 mg GCB 5 mg C18E 4 mg C18E P/N KS-8917 P/N KS-895 GC Columns *All roq Extraction kits contain fifty easy-pour salt packets and fifty 5 ml stand-alone centrifuge tubes. AOAC and EN extraction kits are also available with traditional non-collared 5 ml centrifuge tubes. **All roq dspe kits contain pre-weighed sorbents/salts inside ml or 15 ml centrifuge tubes. Trademarks Kinetex is a registered trademark, roq and Zebron are trademarks of Phenomenex. 14 Phenomenex, Inc. All rights reserved. Core-Shell Technology Magnesium Sulfate(MgSO 4 ) Magnesium Sulfate (MgSO 4 ) Sodium Acetate(NaOAc) Primary/Secondary Amine (PSA) Sodium Chloride(NaCI) Endcapped C18 Sorbent (C18E) Sodium Citrate Tribasic Dihydrate(SCTD) Graphitized Carbon Black (GCB) Sodium Citrate Dibasic Sesquihydrate(SCDS) QuEChERS Tutorial Video Applications QuEChERS FAQ roq QuEChERS Resources Technical Notes Additional Part Numbers and Kits And More. roq QuEChERS Resources and Tools Access All of Your roq Resources /roq Applications Technical Notes Tutorials and Webinars Tools And more roq QuEChERs Selection Poster /roq Choose Your QuEChERS Solution in Under 1 Minute Step 1 Extraction * Step Clean Up/dSPE ** Step 3 Analysis by GC or HPLC AOAC P/N KS-8911 GC ORIGINAL P/N KS-891 P/N KS-891 LC EN P/N KS-899 Salts and Sorbents Used in roq Kits Extraction: Clean Up/dSPE: /roq Intensity, cps App ID 759 /GC /kinetex PO176414_W /roq We re here to help! Speak with your Sample Preparation Specialist By Support@Phenomenex.com For Additional Food Resources Visit /food 14

15 GC Column Selection and Resources! Use the NEW GC Column Finder web app for easy column selection in under 1 minute! Find It The Way You Want Simply choose by part number, manufacturer, application, or official method to find the right GC column for your analysis. Column Selection, Simplified Choose a category, choose your criteria, get a recommendation instantly. Simple as that. Get More Than Just Results Get applications and column overviews with your results and save them for easy viewing. Get it all at /UnmatchedGC 15

16 Food and Environmental Analysis A Quicker and Easier Sample Preparation Choice Australia t: +61 () f: +61 () auinfo@phenomenex.com Austria t: +43 () f: +43 () anfrage@phenomenex.com Belgium t: +3 () (French) t: +3 () (Dutch) f: +31 () beinfo@phenomenex.com Canada t: +1 (8) f: +1 (31) info@phenomenex.com China t: +86 () f: +86 () chinainfo@phenomenex.com Denmark t: f: nordicinfo@phenomenex.com Finland t: +358 () f: nordicinfo@phenomenex.com France t: +33 () f: +33 () franceinfo@phenomenex.com Germany t: +49 () f: +49 () anfrage@phenomenex.com India t: +91 () f: +91 () indiainfo@phenomenex.com Ireland t: +353 () f: eireinfo@phenomenex.com Luxembourg t: +31 () f: +31 () nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: +31 () f: +31 () nlinfo@phenomenex.com New Zealand t: +64 () f: +64 () nzinfo@phenomenex.com Norway t: f: nordicinfo@phenomenex.com Puerto Rico t: +1 (8) 541-HPLC f: +1 (31) info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: +46 () f: nordicinfo@phenomenex.com United Kingdom t: +44 () f: +44 () ukinfo@phenomenex.com USA t: +1 (31) f: +1 (31) info@phenomenex.com All other countries Corporate Office USA t: +1 (31) f: +1 (31) info@phenomenex.com Italy t: f: italiainfo@phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com BR _W Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at /TermsAndConditions. Trademarks Kinetex is registered trademark and SecurityGuard, MidBore, roq, Zebron, Phenex, and Synergi are trademarks of Phenomenex. QTRAP is a registered trademark, Triple Quad, API 3, and API 4 are trademarks of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Disclaimer Comparative separations may not be representative of all applications. SecurityGuard is patented by Phenomenex. U.S. Patent No. 6,16,36 CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP, or ULTRA holders, or to any cartridges. Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,38 and foreign counterparts. The opinions stated herein are solely those of the speaker and not necessarily those of any company or organization. 16 Phenomenex, Inc. All rights reserved.

Extraction and Dispersive Kits User Guide

Extraction and Dispersive Kits User Guide QuEChERS Kits Selection Chart... p. 3 AOAC 2007.01 Method Protocol... p. 4 EN I5662 Method Protocol... p. 5 Original Non-Buffered Protocol... p. 6 Ordering Information...