ph Flexibility Expands Robustness and Reproducibility

Transcription

1 ph Flexibility Expands Robustness and Reproducibility High Efficiency Excellent Lifetime ph Stable -

2 Setting the Standard for ph Method Development Gemini columns are rugged reversed phase HPLC columns that offer extended lifetime at extreme ph conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low ph conditions (ph -) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX technology Phase NX-C C-Phenyl C Description The most rugged Gemini column, offering times the durability of previous generation hybrid columns A low bleed phenyl phase for UV and MS detection, which offers an aromatic selectivity complementary to C phases Selectivity, high structural integrity and increased loadability for preparative and purification applications in pre-packed columns and bulk media USP Classification L L L For analytical and preparative separations of basic and acidic compounds TWIN (Two-In-ne) Technology Gemini C and C-Phenyl During the final stage of silica manufacturing a unique silicaorganic layer is grafted to create a completely new composite particle. nce the internal base silica is unaltered by this manufacturing process, the particle retains its mechanical strength and rigidity along with excellent efficiency, while the silica-organic shell protects the particle from chemical attack. Second-Generation TWIN-NX Technology Gemini NX-C TWIN-NX technology uses an improved patented organosilica grafting process which incorporates highly stabilizing ethane cross-linking. These organic groups are evenly incorporated into the grafted layers on the silica surface while maintaining a pure silica core. This not only provides resistance to high ph attack, but also maintains the high efficiency and mechanical strength of a silica particle. H H H R H R R H R H H R H R R R H R H R R R R H H R H R H R R R R R R R R R R R R R CH CH S CH CH CH CH CH CH CH C CH CH CH CH CH CH CH CH C C CH CH CH CH CH CH CH CH CH Phenomenex, Inc. All rights reserved.

3 How It Works Gemini NX-C Ethane Cross-Linking Resists High ph Attack NX-C ph stable - for durability Consistent performance in both volatile and non-volatile buffers High sample loading capacity for metabolite identification and preparative purification Gemini NX-C USP: L LC/MS Certified ph Stability:.. Particle ze: µm, µm, and μm Phase: C Application: Small molecules, basic compounds Strength: Most durable ph stable particle Pore ze (Å): Surface Area (m /g): Carbon Load %: End Capping: TMS Multi-Point Ligand Attachment Resists Low ph Ligand Cleavage Gemini NX-C Tested for Extreme Durability in Changing Mobile Phase ph Column Efficiencies Maintained in High ph Testing for Cycles Vs. Standard lica lica Dissolution % of Initial Efficiencies Stable Efficiencies After Exposure to, Column Volumes of High and Low ph Buffers Diphenhydramine Cycles of Exposure Tetracaine Retention Times of Four Probes Maintained in Neutral ph Testing for Cycles Ligand Cleavage Retention in Minutes Retention Times Maintained After Exposure to, Column Volumes of High and Low ph Buffers Cycles of Exposure Acetophenone Benzene Toluene Acenaphthene Column Used: Column: Gemini µm NX-C Dimensions: x. mm Part No.: F--E

4 NX-C Polar Bases at Low ph (ph.) Phenomenex Gemini µm NX-C Waters XBridge µm C } } Applications The incredible durability across low and high ph ranges for Gemini NX-C analytical and preparative columns gives scientists the ability to get more performance from their "basic" separations. Polar Bases at High ph (ph.) Phenomenex Gemini µm NX-C Waters XBridge µm C App ID, App ID min min Y-axis normalized for all chromatograms Polar Bases (Beta Blockers) at High ph Dimensions: x. mm Mobile Phase: A: mm Ammonium Bicarbonate ph. B: Acetonitrile Gradient: A/B (:) to (:) in min to (:) in min, Hold for min Flow Rate:. ml/min Detection: nm Sample:. Bisoprolol Contaminant. Sotalol. Atenolol. Labetalol (Diastereoisomeric Pair). Nadolol (Diastereoisomeric Pair). Pindolol. Metoprolol. Bisoprolol. Propranolol. Alprenolol App ID, App ID min min Y-axis normalized for all chromatograms Polar Bases (Antihistamines) in Formic Acid Dimensions: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Gradient: A/B (:) to (:) in min Flow Rate:. ml/min Detection: nm Sample:. Pyrilamine. Tripelennamine. Chlorpheniramine. Brompheniramine. Chloropyramine. Diphenhydramine. Loratadine Comparative separations may not be representative of all applications.

5 NX-C Applications Hydrophobic Basic Drugs at Low ph (ph.) Phenomenex Gemini µm NX -C Waters XBridge µm C App ID App ID min min Y-axis normalized for all chromatograms Agilent Technologies ZRBAX µm Extend-C Thermo Scientific Hypersil GLD µm C App ID App ID min min Y-axis normalized for all chromatograms Hydrophobic Bases (Diltiazem, Promethazine) in Formic Acid Columns: Gemini µm NX-C XBridge µm C ZRBAX µm Extend-C Hypersil GLD µm C Dimensions: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Gradient: A/B (:) to (:) in min Flow Rate:. ml/min Detection: nm Sample:. Diltiazem. Promethazine Comparative separations may not be representative of all applications.

6 NX-C Applications Performance in Volatile Buffers With the widespread adoption of LC/MS and LC/MS/MS techniques, column performance in volatile buffers is critical. Phenomenex Gemini µm NX-C Waters XBridge µm C App ID App ID min min Y-axis normalized for all chromatograms Agilent Technologies ZRBAX µm Extend-C Thermo Scientific Hypersil GLD µm C, App ID App ID min min Y-axis normalized for all chromatograms Mixtures of Acids, Neutrals, and Bases in Formic Acid (ph.) Columns: Gemini µm NX-C XBridge µm C ZRBAX µm Extend-C Hypersil GLD µm C Dimensions: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Gradient: A/B (:) to (:) in min, Hold for min Flow Rate:. ml/min Detection: nm Sample:. Pyridine. Quinidine. Sulfathiazole. Triprolidine. Benzyl alcohol. Nortriptyline. Phenol. -Methyl--nitrobenzoic acid. Methylsalicylaldehyde. Hexanophenone Comparative separations may not be representative of all applications.

7 NX-C Applications Performance in Non-Volatile Buffers Gemini was engineered to be the column of choice for pharmaceutical scientists who work with difficult sample mixtures and harsh mobile phase conditions. The consistent performance and rugged phase will provide simplified method development and long column lifetimes. Phenomenex Gemini µm NX-C Waters XBridge µm C App ID App ID min min Y-axis normalized for all chromatograms Agilent Technologies ZRBAX µm Extend-C Thermo Scientific Hypersil GLD µm C,, App ID App ID min min Sample:. Pyridine. Quinidine. Sulfathiazole. Triprolidine. Benzyl alcohol Y-axis normalized for all chromatograms Mixtures of Acids, Neutrals, and Bases in Potassium Phosphate (ph.) Columns: Gemini µm NX-C XBridge µm C ZRBAX µm Extend-C Hypersil GLD µm C Dimensions: x. mm Mobile Phase: A: mm Potassium Phosphate ph. B: Acetonitrile Gradient: A/B (:) to (:) in min, Hold for min Flow Rate:. ml/min Detection: nm. Phenol. Nortriptyline. -Methyl--nitrobenzoic acid. Methylsalicylaldehyde. Hexanophenone Comparative separations may not be representative of all applications.

8 C Increased loading and retention of basic compounds lica efficiency and mechanical strength ph stable for durability Chromatographic Comparisons Phenomenex Gemini µm C Gemini C USP: L LC/MS Certified ph Stability:.. Particle ze: µm, µm, and μm Phase: C Application: Small molecules, basic compounds Strength: Wide ph stability, high efficiency Pore ze (Å): Surface Area (m /g): Carbon Load %: End Capping: TMS Agilent Technologies ZRBAX µm Extend-C Virtually no silanol interactions! min Tricyclic Antidepressants at Neutral ph Dimensions: x. mm Mobile Phase: mm Phosphate buffer, ph./acetonitrile/ Methanol (::) Flow Rate:. ml/min Detection: nm Sample:. Nortriptyline. Imipramine. Amitriptyline. Clomipramine App ID min Advanced Chromatography Technologies ACE µm C min App ID App ID Batch-to-Batch Reproducibility Extended Column Lifetime The TWIN Technology engineering of Gemini provides stability and increased column lifetime. Whether used under isocratic or gradient conditions, Gemini out-performs and outlasts ph stable columns. This is illustrated below. App ID Lifetime and Efficiency Comparison. times increased column lifetime and still going! Batch Batch Batch Batch min Efficiency (N) Columns fail after % loss in efficiency. Gemini Fail Line XTerra Fail Line Conditions for all separations: Column: Gemini µm C Dimension: x. mm Part No.: F--E Mobile Phase: mm Ammonium Bicarbonate, ph./ Acetonitrile (:) Flow Rate:. ml/min Detection: nm Sample:. Pindolol. Metoprolol. Propranolol Days Efficiency and lifetime comparison based on average of two columns each run in parallel. Columns: Gemini µm C Waters XTerra µm MS C Dimensions: x. mm Mobile Phase: Acetonitrile/ mm Methylpyrrolidine Buffer, ph. (:) Flow Rate: ml/min Detection: nm Sample: Diphenhydramine Gemini C Waters XTerra MS C Comparative separations may not be representative of all applications.

9 C-Phenyl ph stable - for durability Great aromatic selectivity Extremely low UV and MS bleed Gemini C-Phenyl USP: L LC/MS Certified ph Stability:.. Particle ze: µm and µm Phase: Phenyl with C linker Application: Aromatic, polar or basic compounds Strength: High aromatic selectivity with exceptional peak shape even in neutral conditions. Extremely low bleed phenyl column. Pore ze (Å): Surface Area (m /g): Carbon Load %: End Capping: TMS Enhanced Performance for Aromatic Compounds Sulfa Drug Application Pursuit µm Diphenyl Gemini µm C-Phenyl Resolution Rs,.. Rs,.. Dimensions: x. mm Mobile Phase:. % Formic Acid in Water/ Methanol (:) Flow Rate:. ml/min Detection: nm Sample:. Sulfathiazole. Sulfamerazine. Sulfamethoxazole mau mau mau mau Phenomenex Gemini µm C-Phenyl Agilent Technologies Pursuit μm Diphenyl App ID min min App ID min min Reproducible Phenyl Phase Low Bleed Phenyl Phase Aliphatic Acid Application.e.e Popular C Intensity cps.e.e.e.e.e.e.e Gemini C-Phenyl Blank min. min Column: Gemini µm C-Phenyl Dimensions: x. mm Part No.: F--E Mobile Phase: mm Phosphate buffer, ph./methanol (:) Flow Rate:. ml/min Detection: nm Sample:. Tartaric Acid. Acetic Acid. Malic Acid. Citric Acid. Lactic Acid. Propionic Acid Dimensions: x. mm Mobile Phase: A:. % Formic acid in Water B:. % Formic acid in Acetonitrile Gradient: % B to % B in min, then hold % B for min Flow Rate:. ml/min MS Detection: ESI + ion mode, M/Z - Comparative separations may not be representative of all applications.

10 Seamlessly Scale-Up to Gemini Axia Packed Prep Columns Axia Packing Technology Axia packed preparative columns involve a single axial compression step unlike conventional packed preparative columns. The ideal column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time. During the Axia packing process, the packing piston is locked in place, eliminating any decompression and then recompression of the media sorbent, thus maintaining media and column bed integrity. This solves common lifetime and performance problems associated with conventional packing processes for preparative columns. Axia Packing Process Involves: Compression Final Column The packing piston head is integrated into the column and locked by the piston retainers, so the pressure is never released. Media packed under ideal pressure Media is never allowed to relax, eliminating voids and dramatically improving reproducibility, column-to-column. Axia Technology vs. Traditional BD Prep Column Packing Traditional Slurry Packing Traditional slurry packing processes, like the Waters BD (ptimum Bed Density) column packing approach, involve the column being removed from the column packing station once it is packed. Several potential problems with this packing method are: Variability in column performance due to increased number of manual operations required for assembly Potential silica media damage during recompression Level of process control is based on traditional slurry packing technology Conventional Packing Process Involves: Compression Decompression Recompression Final Column Pump Column Media escapes from disconnected column Removal of escaped media Media density disruption and potential media crushing lica media packed under liquid pressure Violent recompression of silica media Diagram from Waters Corporation U.S. Patent No.,,

11 See the Difference with Gemini Axia Packed Prep Columns Axia packed columns produce uniform media bed with intact particles The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed prep columns. *SEM of Axia inlet frit Intact media at frit surface after packing *SEM of Waters BD inlet frit Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles Decompression and then recompression during packing can damage the media and lead to increased columnto-column variability, flow disturbances, and decreased column lifetimes. Crushed media or silica fines at frit surface after packing *The images are believed to be representative, but individual columns may vary. Dramatically improve sample resolution, productivity and performance of any preparative column media with Axia column hardware and packing technology. Axia packed prep column offers the opportunity for longer lifetime, higher loading and increased throughput. Phenomenex Gemini µm NX-C Axia Packed Waters XBridge µm C Prep BD % increase in resolution App ID App ID min min Conditions for both columns: Column: Gemini µm NX-C Axia Packed Waters µm XBridge Dimensions: x. mm (Gemini) x mm (XBridge) Mobile Phase: A: mm Ammonium bicarbonate ph. B: Acetonitrile Gradient: Time (min) % B.... Flow Rate: ml/min Temperature: C Detection: nm Sample:. Reserpine. Unknown Key: Best Suited Very Good Packing Material Particle ze (µm) Pore ze (Å) Surface Area (m /g) Carbon Load (%) ph Range Gemini C,,.-. Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Gemini C-Phenyl,.-. Gemini NX-C,,.-. Comparative separations may not be representative of all applications.

12 ph Flexibility Allows for Increased Purification Performance Separating basic compounds at higher ph levels produces dramatic changes when compared to low ph conditions. At ph., the basic compounds become neutralized and are more hydrophobic. The retention for the uncharged basic compounds increases providing an increase in separation along with superior peak shapes. Gemini NX-C with. % TFA Gemini NX-C with. % NH H Low ph High ph.. mg in µl App ID. mg in µl App ID min min Column: Gemini µm NX-C Axia Packed Dimensions: x. mm Mobile Phase: A:. % TFA in Water B: Acetonitrile Gradient: % to % B in min Flow Rate: ml/min Detection: nm Sample:. Diphenhydramine. Propranolol Column: Gemini µm NX-C Axia Packed Dimensions: x. mm Mobile Phase: A:. % NH H in Water B: Acetonitrile Gradient: % to % B in min Flow Rate: ml/min Detection: nm Sample:. Diphenhydramine. Propranolol Separation shape improvement provides opportunity for increased loading Gemini NX-C with. % TFA Gemini NX-C with. % NH H Low ph App ID High ph. App ID mg in µl min Column: Gemini µm NX-C Axia Packed Dimensions: x. mm Mobile Phase: A:. % TFA in Water B: Acetonitrile Gradient: % to % B in min Flow Rate: ml/min Detection: nm Sample:. Diphenhydramine. xybutynin. Terfenadine mg in µl min Column: Gemini µm NX-C Axia Packed Dimensions: x. mm Mobile Phase: A:. % NH H in Water B: Acetonitrile Gradient: % to % B in min Flow Rate: ml/min Detection: nm Sample:. Diphenhydramine. xybutynin. Terfenadine ur Phenomenex Gemini and Luna Axia packed columns are the workhorses in our lab. These columns exhibit outstanding performance for challenging separations while also handling a high workload for standard separations. Longevity has also been excellent with some columns lasting years or more. Dependability is so important in my line of work and these columns never disappoint!! -Major Pharmaceutical Company, USA Tip: If you want longer Gemini NX-C Axia packed column lifetimes, request technical note: TN- Increase Column Performance and Lifetime in Peptide and Protein Purification using Aggressive Wash Conditions

13 Core-Shell Technology For Far Greater Performance Use Kinetex Core-Shell Technology! Kinetex EV C: Superior Core-Shell Upgrade Higher sensitivity and efficiency without increase in backpressure Superior peak shape for bases Greater cost savings The unique organo-silica layer of ethane cross-linking found within each Kinetex EV C particle creates a highly inert surface which provides the additional benefit of better peak shape for bases. With the combination of rugged ph stability from - and the core-shell performance advantage, you can easily replace old hybrid silica columns and gain immediate method improvements without increasing backpressure! UHPLC HPLC PREP. µm % higher efficiency than fully porous. µm columns. µm Achieve sub- µm performance on HPLC and UHPLC systems µm µm or better efficiencies at µm pressures for HPLC and PREP LC methods When Compared to Waters XBridge C: Phenomenex Kinetex µm EV C Waters XBridge. µm C mau mau Pressure: bar Pressure: bar EV APP ID APP ID min min What an increase in sensitivity and peak shape! Waters XBridge µm C Column: Kinetex µm EV C XBridge µm C XBridge. µm C Dimensions: x. mm Mobile Phase: A:. % Formic acid in Water B:. % Formic acid in Acetonitrile Gradient: % to % B over minutes Flow Rate:. ml/min Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--Nitrobenzoic acid. -Methyl Salicyl Aldehyde. Hexaphenone Pressure: bar APP ID min Comparative separations may not be representative of all applications. For rdering Information and to Learn More about the Kinetex Family visit:

14 HPLC Column Protection Protect your Columns from Contaminants Extends HPLC column lifetime Won t alter chromatography Compatible with virtually all HPLC columns Easy to determine when to change cartridge mple to use How Does It Work? The SecurityGuard analytical cartridge holder (patented) directly fingertightens into virtually any manufacturer s column with a female/inverted endfitting. Contaminants are retained by an inexpensive disposable cartridge instead of damaging your valuable HPLC column investment. Cut-away view showing cartridge can be easily inspected for contaminants Increases HPLC Column Lifetime, Guaranteed! mply replace SecurityGuard cartridges instead of your expensive HPLC columns. In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use. Backpressure (Bar) Cartridge Replaced From Injector HPLC Column to Detector - Column returns to initial backpressure Injections Accelerated lifetime test using endogenous biomolecule matrix on a reversed phase C column, µm, x. mm with SecurityGuard C cartridges. Backpressure values represent additional backpressure contributed by SecurityGuard. Universal fingertight quick connection to HPLC column no wrenches required See it in Action! Visit:

15 rdering Information μm Microbore, Minibore and MidBore Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x. x. x. x. x. x.* /pk C B--A M--B A--B B--B D--B F--B B--Y D--Y F--Y AJ- C-Phenyl B--A A--B B--B D--B F--B B--Y D--Y F--Y AJ- /pk NX-C B--A M--B A--B B--B D--B F--B B--Y D--Y F--Y AJ- for ID:.-. mm μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x. x. x.* /pk C M--D A--E B--E D--E F--E G--E AJ- C-Phenyl A--E B--E D--E F--E G--E AJ- /pk NX-C B--E D--E F--E G--E AJ- for ID:.-. mm μm Minibore and MidBore Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x. x. x. x. x.* /pk C A--B B--B F--B G--B B--Y D--Y F--Y G--Y AJ- C-Phenyl A--B B--B F--B B--Y F--Y G--Y AJ- /pk NX-C A--B B--B F--B B--Y D--Y F--Y G--Y AJ- μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x. x.* /pk C A--E B--E D--E F--E G--E AJ- C-Phenyl B--E D--E F--E G--E AJ- /pk NX-C B--E D--E F--E G--E AJ- for ID:.-. mm μm Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases x x x /pk C F--N G--N AJ- C-Phenyl G--N AJ- /pk NX-C F--N G--N AJ- for ID: - mm Axia Packed Preparative Columns (mm) for ID:.-. mm For Gemini Capillary HPLC Columns, Guards, and Adapter, contact your Phenomenex technical consultant or local distributor. SecurityGuard Cartridges (mm) Phases x. x. x. x. x x x.** x. μm ea ea C B--P-AX D--P-AX F--P-AX G--P-AX B--U-AX C--U-AX AJ- AJ- C-Phenyl D--P-AX F--P-AX G--P-AX C--U-AX AJ- AJ- μm ea ea NX-C B--P-AX D--P-AX F--P-AX G--P-AX B--U-AX C--U-AX AJ- AJ- μm ea ea C B--P-AX D--P-AX F--P-AX G--P-AX AJ- AJ- μm ea ea NX-C B--P-AX D--P-AX F--P-AX G--P-AX AJ- AJ- for ID: - mm - mm Axia Packed Preparative Columns (mm) continued Axia Packed Preparative Columns (mm) continued SecurityGuard Cartridges (mm) Phases x x x x x x x x. μm Inquire Inquire Inquire ea C D--U-AX F--U-AX G--U-AX B--V-AX AJ- C-Phenyl D--U-AX AJ- μm Inquire Inquire ea NX-C D--U-AX F--U-AX G--U-AX AJ- μm Inquire Inquire Inquire Inquire Inquire Inquire ea C D--U-AX F--U-AX G--U-AX B--V-AX D--V-AX F--V-AX G--V-AX AJ- μm Inquire Inquire Inquire Inquire Inquire Inquire ea NX-C D--U-AX F--U-AX G--U-AX B--V-AX D--V-AX F--V-AX G--V-AX AJ- for ID: - mm If Gemini analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within days for a full refund. *SecurityGuard Analytical Cartridges require holder, Part No.: KJ- SecurityGuard SemiPrep Cartridges require holder, Part No.: AJ- ** SecurityGuard PREP Cartridges require holder, Part No.: AJ- SecurityGuard PREP Cartridges require holder, Part No.: AJ-

16 ph Flexibility Expands Robustness and Reproducibility Australia t: + ()-- f: + ()-- auinfo@phenomenex.com Austria t: + ()-- f: + ()-- anfrage@phenomenex.com Belgium t: + () (French) t: + () (Dutch) f: + ()- beinfo@phenomenex.com Canada t: + () - f: + () - info@phenomenex.com China t: + () - f: + () - chinainfo@phenomenex.com Denmark t: + f: + nordicinfo@phenomenex.com Finland t: + () f: + nordicinfo@phenomenex.com France t: + () f: + () franceinfo@phenomenex.com Germany t: + ()-- f: + ()-- anfrage@phenomenex.com India t: + ()- f: + ()- indiainfo@phenomenex.com Ireland t: + () f: + - eireinfo@phenomenex.com Luxembourg t: + ()- f: + ()- nlinfo@phenomenex.com Mexico t: --- f: --- tecnicomx@phenomenex.com The Netherlands t: + ()- f: + ()- nlinfo@phenomenex.com New Zealand t: + ()- f: + ()- nzinfo@phenomenex.com Norway t: + f: + nordicinfo@phenomenex.com Puerto Rico t: + () -HPLC f: + () - info@phenomenex.com Spain t: + -- f: + -- espinfo@phenomenex.com Sweden t: + () f: + nordicinfo@phenomenex.com United Kingdom t: + ()- f: + ()- ukinfo@phenomenex.com USA t: + () - f: + () - info@phenomenex.com All other countries Corporate ffice USA t: + () - f: + () - info@phenomenex.com Italy t: + f: + italiainfo@phenomenex.com Terms and Conditions Subject to Phenomenex Standard Terms and Conditions which may be viewed at Trademarks Gemini, Kinetex, and Phenomenex are registered trademarks of Phenomenex. Axia, MidBore, SecurityGuard, TWIN Technology, and TWIN-NX are trademarks of Phenomenex. Hypersil GLD is a registered trademark of Thermo Hypersil-Keystone, Inc. Thermo Scientific is a registered trademark of Thermo Fisher Scientific, Inc. Agilent, Agilent Technologies, Pursuit, and ZRBAX are registered trademarks of Agilent Technologies, Inc. ACE is a registered trademark of Advanced Chromatography Technologies (ACT). Waters, XBridge, and XTerra are registered trademarks and BD is a trademark of Waters Corporation. Disclaimers Comparative separations may not be representative of all applications. Phenomenex is in no way affiliated with Agilent Technologies, Waters Corporation, Thermo Fisher Scientific, or Advanced Chromatography Technologies (ACT). Axia column and packing technology is patented by Phenomenex. U.S. Patent No,,. Gemini and Kinetex EV C are patented by Phenomenex. U.S. Patent Nos.,, and,, and foreign counterparts. Phenomenex, Inc. All rights reserved. BR_W Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com