MOLECULAR BIOLOGY GREATEST HITS. Marketplace. Essentials Tour molecular biology. thermofisher.com/marketplace

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Thermo Scientific products: Cloning kits

X-Gal DNA ladders Agarose tablets Nucleic

transcriptases DNA polymerases PCR plastic

1 molecular biology Marketplace MOLECULAR BIOLOGY GREATEST HITS Special offers on Thermo Scientific products: Cloning kits Restriction and modifying enzymes IPTG and X-Gal DNA ladders Agarose tablets Nucleic acid purification kits Reverse transcriptases DNA polymerases PCR plastic consumables dntp solutions Essentials Tour 2016 thermofisher.com/marketplace

Essential cloning tools Thermo Scientific FastDigest Restriction Enzymes Experience the first restriction enzyme system with one universal buffer 100% activity of all FastDigest enzymes in one

enzymes at: thermofisher.com/fastdigest * Use promotion code RMD325 and refer to the back cover for additional terms and conditions and ordering details.

K1991 High efficiency cloning kits Thermo Scientific Cloning Kits PCR cloning of blunt- and sticky-end DNA fragments in 5 10 minutes (Cat. Nos.

2 Essential cloning tools Thermo Scientific FastDigest Restriction Enzymes Experience the first restriction enzyme system with one universal buffer 100% activity of all FastDigest enzymes in one universal buffer Designed to complete digestion in 5 15 minutes Direct loading on gels with FastDigest green buffers 100% buffer compatibility with downstream applications Discover all 176 FastDigest enzymes at: thermofisher.com/fastdigest * Use promotion code RMD325 and refer to the back cover for additional terms and conditions and ordering details. FastDigest Value Pack contains the 13 most commonly used FastDigest enzymes. Cat. No. K1991 High efficiency cloning kits Thermo Scientific Cloning Kits PCR cloning of blunt- and sticky-end DNA fragments in 5 10 minutes (Cat. Nos. K1231/2) Ligation of blunt- or sticky-end DNA fragments in 5 minutes (Cat. Nos. K1422/3) K reactions CloneJET PCR Cloning Kit K reactions K reactions Rapid DNA Ligation Kit K reactions DNA modifying enzymes Thermo Scientific DNA Modifying Enzymes High processivity and strand displacement activity; for applications including whole genome amplification or sequencing (Cat. Nos. EP0094/2) Catalyzes 5 3 synthesis from primed single-stranded DNA; for blunting of DNA overhangs (Cat. Nos. EP0061/2) Dephosphorylates all types of DNA ends in 10 minutes at 37 C (Cat. Nos. EF0651/2) Active in PCR buffer and degrades single-stranded DNA in a 3 5 direction; for efficient PCR clean-up (Cat. Nos. EN0581/2) EP0094 5,000 U Phi29 DNA Polymerase (10 U/µL) EP0092 1,000 U EP U T4 DNA Polymerase (5 U/µL) EP U EF0651 1,000 U FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) EF x 1,000 U EN0581 4,000 U Exonuclease I (EXOI) (20 U/µL) EN ,000 U

Blue/white colony screening tools Thermo Scientific IPTG and X-Gal Isopropyl-beta-D-thiogalactopyranoside (IPTG) is a highly stable inducer of gene

R0941) 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-Gal) is an inert chromogenic substrate of B-galactosidase used with IPTG for colorimetric

5 ml R0404 1 g X-Gal R0402 2 x 1 g R0941 X-Gal Solution, ready-to-use 10 ml Streamline cloning for successful protein expression The Thermo Scientific

Experience high level cloning and robust expression of different proteins varying in size, origin, and toxicity. Learn more at thermofisher.

to 13 kb Optimum activity at 42 C, active up to 55 C for low- and high-complexity RNA samples EP0441 10,000 U RevertAid Reverse Transcriptase EP0442 5 x

RevertAid H Minus First Strand cdna Synthesis Kit 100 reactions K1691 RevertAid RT Reverse Transcription Kit 500 reactions Did you know?

3 Blue/white colony screening tools Thermo Scientific IPTG and X-Gal Isopropyl-beta-D-thiogalactopyranoside (IPTG) is a highly stable inducer of gene expression from the lac operon; used in conjunction with X-gal for colorimetric screening of recombinant clones (Cat. No. R0941) 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-Gal) is an inert chromogenic substrate of B-galactosidase used with IPTG for colorimetric selection of positive clones R g IPTG, dioxane-free R x 5 g R1171 IPTG Solution, ready-to-use 10 x 1.5 ml R g X-Gal R x 1 g R0941 X-Gal Solution, ready-to-use 10 ml Streamline cloning for successful protein expression The Thermo Scientific alicator LIC Cloning and Expression System is designed for fast and efficient cloning with subsequent tightly regulated gene expression in E. coli. Experience high level cloning and robust expression of different proteins varying in size, origin, and toxicity. Learn more at thermofisher.com/alicator Essential tools for reverse transcription Thermo Scientific RevertAid Reverse Transcriptases (RT) Efficient synthesis of full-length cdna up to 13 kb Optimum activity at 42 C, active up to 55 C for low- and high-complexity RNA samples EP ,000 U RevertAid Reverse Transcriptase EP x 10,000 U K1622 RevertAid First Strand cdna Synthesis Kit 100 reactions EP ,000 U RevertAid H Minus Reverse Transcriptase EP x 10,000 U K1632 RevertAid H Minus First Strand cdna Synthesis Kit 100 reactions K1691 RevertAid RT Reverse Transcription Kit 500 reactions Did you know? The polymerase that binds You can choose from a variety of high-quality predesigned primers for your application. Our products range from exonuclease-resistant primers for cdna synthesis to commonly used primers for sequencing of cloned DNA inserts. Go to thermofisher.com/tsprimers

2 ml R0192 dntp Mix, 10 mm each 1 ml R0193 5 x 1 ml R1121 1 ml dntp Mix, 25 mm each R1122 5 x 1 ml R0181 4 x 0.

4 High-purity dntps Born to clone Thermo Scientific dntp Set and Mix Greater than 99% purity confirmed by HPLC Free of contaminating human and E. coli DNA Highly stable designed to prevent loss of performance after multiple freeze/thaw cycles R ml R0192 dntp Mix, 10 mm each 1 ml R x 1 ml R ml dntp Mix, 25 mm each R x 1 ml R x 0.25 ml dntp Set, 100 mm Solutions R x 1 ml Cloning in the dark Plastic consumables for PCR and qpcr 40% Thermo Scientific Armadillo PCR plates Clean-room production certified free from DNA, RNases, and DNases Plates, strips, and sealing options compatible with all main PCR and PCR instruments AB-0558 Adhesive PCR Plate Seals 100 sheets AB-0626 Adhesive PCR Plate Foils 100 sheets AB-0784 Strips of 8 Flat PCR Caps 250 strips AB-0866 Ultra Clear Cap Strips 120 strips AB-1170 Absolute qpcr Plate Seals 50 seals AB-0600-L 96-well PCR Plate, Non-Skirted, Black Lettering 25 plates AB-0700-L 96-well Low Profile PCR Plate, Non-Skirted, Black Lettering 25 plates AB-0800-L 96-well Low Profile PCR Plate, Skirted, Black Lettering 25 plates AB-0800/B-L 96-well Low Profile PCR Plate, Blue, Skirted, Black Lettering 25 plates AB-0800/G-L 96-well Low Profile PCR Plate, Green, Skirted, Black Lettering 25 plates AB-0800/P-L 96-well Low Profile PCR Plate, Purple, Skirted, Black Lettering 25 plates AB-0800/R-L 96-well Low Profile PCR Plate, Red, Skirted, Black Lettering 25 plates AB-0800/W-L 96-well Low Profile PCR Plate, White, Skirted, Black Lettering 25 plates AB-0800/Y-L 96-well Low Profile PCR Plate, Yellow, Skirted, Black Lettering 25 plates AB-1400-L 96-well PCR Plate, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/B-L 96-well PCR Plate, Blue, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/G-L 96-well PCR Plate, Green, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/P-L 96-well PCR Plate, Purple, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/R-L 96-well PCR Plate, Red, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/W-L 96-well PCR Plate, White, Semi-Skirted, Flat Deck, Black Lettering 25 plates AB-1400/Y-L 96-well PCR Plate, Yellow, Semi-Skirted, Flat Deck, Black Lettering 25 plates * Use promotion code RMD340 and refer to the back cover for additional terms and conditions and ordering details. My clonetown Streets of dntps Did you know? For over 25 years, we ve been supplying our comprehensive range of high-quality Thermo Scientific plastics for molecular biology research. Our PCR plastics are designed, manufactured, and tested to help ensure optimal PCR performance. Learn more at thermofisher.com/thermoscientificplastics The PCR plastic shuffle

Essential electrophoresis tools Agarose road Better gels Thermo Scientific GeneRuler DNA Ladders Broad-range ladders with bright reference bands that allow easy quantification of DNA fragments

proprietary process that eliminates the use of organic solvents Range SM0241 100 appl 100 1,000 GeneRuler 100 bp DNA Ladders SM0242 500 appl SM0243 GeneRuler 100 bp DNA Ladder, ready-to-use 100 appl

2,500 appl SM0313 GeneRuler 1 kb DNA Ladder, ready-to-use 500 appl SM1331 500 appl 75 20,000 GeneRuler 1 kb Plus DNA Ladder SM1332 2,500 appl SM1333 GeneRuler 1 kb Plus DNA Ladder, ready-to-use 500

GeneJET Nucleic Acid Purification Kits High yields of pure nucleic acids Fast protocols Convenient spin columns with trusted silica membrane technology K0502 50 preps GeneJET Plasmid Miniprep Kit

5 Essential electrophoresis tools Agarose road Better gels Thermo Scientific GeneRuler DNA Ladders Broad-range ladders with bright reference bands that allow easy quantification of DNA fragments Supplied with 6X loading dye Thermo Scientific TopVision Agarose Tablets Convenient tablet format with fast dissolving protocol Helps enhance resolution and reduce background Manufactured using a proprietary process that eliminates the use of organic solvents Range SM appl 100 1,000 GeneRuler 100 bp DNA Ladders SM appl SM0243 GeneRuler 100 bp DNA Ladder, ready-to-use 100 appl SM appl 100 3,000 GeneRuler 100 bp Plus DNA Ladder SM appl SM0323 GeneRuler 100 bp Plus DNA Ladder, ready-to-use 100 appl SM appl ,000 GeneRuler 1 kb DNA Ladder SM0312 2,500 appl SM0313 GeneRuler 1 kb DNA Ladder, ready-to-use 500 appl SM appl 75 20,000 GeneRuler 1 kb Plus DNA Ladder SM1332 2,500 appl SM1333 GeneRuler 1 kb Plus DNA Ladder, ready-to-use 500 appl R0611 6X DNA Loading Dye 5 x 1 ml R0631 Orange 6X DNA Loading Dye 5 x 1 ml R tablets TopVision Agarose Tablets R2802 1,000 tablets Nucleic acid purification kits Thermo Scientific GeneJET Nucleic Acid Purification Kits High yields of pure nucleic acids Fast protocols Convenient spin columns with trusted silica membrane technology K preps GeneJET Plasmid Miniprep Kit K preps K preps GeneJET Plasmid Maxiprep Kit K preps K preps GeneJET Gel Extraction Kit K preps K preps GeneJET PCR Purification Kit K preps We shall synthesize

Phusion days Superior cloning with high-fidelity PCR Thermo Scientific Phusion High-Fidelity DNA Polymerases Very accurate and fast amplification with high inhibitor tolerance Robust performance and

High-Fidelity PCR Master Mix with HF Buffer F-531L 500 x 50 μl reactions F-549S 100 U Phusion Hot Start II High-Fidelity DNA Polymerase F-549L 500 U F-532S 100 x 50 μl reactions Phusion High-Fidelity

6 Phusion days Superior cloning with high-fidelity PCR Thermo Scientific Phusion High-Fidelity DNA Polymerases Very accurate and fast amplification with high inhibitor tolerance Robust performance and high yields with minimal enzyme amounts Direct loading on gels with Phusion Green formats F-530S 100 U Phusion High-Fidelity DNA Polymerase F-530L 500 U F-531S 100 x 50 μl reactions Phusion High-Fidelity PCR Master Mix with HF Buffer F-531L 500 x 50 μl reactions F-549S 100 U Phusion Hot Start II High-Fidelity DNA Polymerase F-549L 500 U F-532S 100 x 50 μl reactions Phusion High-Fidelity PCR Master Mix with GC Buffer F-532L 500 x 50 μl reactions F-548S 100 x 20 µl reactions Phusion Flash High-Fidelity PCR Master Mix F-548L 500 x 20 µl reactions * Use promotion code RMD325 and refer to the bottom of this page for additional terms and conditions and ordering details. Did you know? You can minimize errors and help save time in your PCR setup by calculating volumes with the Thermo Scientific Phusion reaction setup calculator? Learn more at thermofisher.com/phusionsetup Bio Sciences Ltd - info@biosciences.ie - For further information and ordering, go to thermofisher.com/marketplace *Discount will apply to qualifying orders received by Thermo Fisher Scientific between September 1, 2016, and December 31, 2016, or until promotional supplies are depleted, whichever comes first. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. For ordering information and technical support, go to thermofisher.com/contactus For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. COL02509-T2

Marketplace. Essentials Tour The bright side of PCR. molecular biology. thermofisher.com/marketplace

Marketplace. Essentials Tour The bright side of PCR. molecular biology. thermofisher.com/marketplace molecular biology Marketplace The bright side of PCR Special offers on Thermo Scientific products: Agarose powder DNA ladders Electrophoresis dyes Enhanced Taq DNA polymerases High-fidelity DNA polymerases