AP Biology: Gene Mapping
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1 AP Biology: Gene Mapping Name: 1. The crossover frequency between genes E and F is 6%, between E and G is 10% and between F and G is 4%. Construct a gene map. Determine the sequence of genes on the chromosome. 2. A, B, and C are linked genes. A testcross shows that the recombination frequencies between A and B is 28%, and between A and C is 12%. Can you determine the linear order of these genes? Explain. 3. In 1911, Thomas Morgan collected the following crossover gene frequencies while studying Drosophila. Barshaped eyes are indicated by the B allele, and carnation eyes are indicated by the allele C. Fused veins on wings (FV) and scalloped wings (S) are also located on the same chromosome. Gene Combinations Frequencies of Recombinations FV/B 2.5% FV/C 3.0% B/C 5.5% B/S 5.5% FV/S 8.0% C/S 11.0% Determine the sequence of genes on the chromosome. 4. Determine the sequence of genes along a chromosome based on the following recombination frequencies: a. A-B = 8% b. A-C = 28% c. A-D = 25% d. B-C = 20% e. B-D= 33%
2 5. The following chart shows crossover frequencies for some genes on an autosome of organism Z. Construct a chromosome map. Order the genes on the chromosome. P & Q 5% P & R 8% P & S 12% Q & R 13% Q & S 17% 6. For a series of experiments, a linkage group composed of genes W, X, Y and Z was found to show the following gene combinations. (All recombinations are expressed per 100 fertilized eggs). Construct a gene map. Determine the sequence of genes on the chromosome. W X Y Z W X Y Z For a series of experiments, a linkage group composed of genes A, B, C and D was found to show the following gene combinations. (All recombinations are expressed per 100 fertilized eggs). Construct a gene map. Determine the sequence of genes on the chromosome. A B C D A B C D
3 8. Use the table below to map the genes found on this chromosome of a fruit fly. 9. In Drosophila, the genes for eye colour (pr), wing shape (vg), and body colour (eb) are all found on the same chromosome. The following crossover frequencies for these genes were determined by experimentation. pr & vg 12.5% pr & eb 6.0% vg & eb 18.5% Construct a gene map. Determine the sequence of genes on the chromosome. 10. Using the following chart, construct a gene map and determine the sequence of genes on the chromosome. A & B 24.0% A & C 8.0% C & D 2.0% A & F 16.0% F & B 8.0% D & F 6.0%
4 11. Five traits found in Drosophila on the same chromosome are yellow body colour (y), white eye colour (w), vermilion eye colour (v), miniature wing (m) and rudimentary wing (r). Given the following crossover frequencies, construct a gene map and correctly order the genes on this chromosome. y & w 2.2% v & m 3.0% v & r 26.6% v & w 30.0% v & y 32.2% w & m 33.0% y & m 35.2% w & r 56.6% 12. Determine the sequence of genes along a chromosome based on the following recombination frequencies: a. A-B = 30% b. A-C = 9% c. A-D = 18% d. B-C = 21% e. B-D = 12% f. E-D = 12% g. E-A = 6% h. E-C = 3% i. E-B = 24%
5 CHALLENGE A space probe discovers a planet inhabited by creatures who reproduce with the same hereditary patterns seen in humans. Three phenotypic characters are height (T = tall, t = short), head appendages (A = antennae, a = no antennae), and nose morphology (S = upturned snout, s = downturned snout). Earth scientists are able to do some controlled breeding experiments on them using various heterozygotes. For a tall heterozygote with antennae cross, the offspring are: tall/antennae - 46 dwarf/antennae - 7 dwarf/no antennae - 42 tall/no antennae - 5 For a heterozygote cross with antennae and an upturned snout the offspring were: antennae/upturned - 47 antennae/downturned snout - 2 no antennae/downturned snout - 48 no antennae/upturned snout -3 Calculate the recombination frequencies for each. A further testcross is done using a heterozygote for height and nose morphology. The offspring are... Tall-upturned: 40 Short-upturned: 9 Tall-downturned: 9 Short-downturned: 42 Calculate the recombination frequency from this data. Now, determine the sequence of genes along a chromosome with these recombinant frequencies.
AP Biology: Gene Mapping
AP Biology: Gene Mapping Name: 1. The crossover frequency between genes E and F is 6%, between E and G is 10% and between F and G is 4%. Construct a gene map. Determine the sequence of genes on the chromosome.
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