Andreas Sebastian Klein, M. Sc.
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1 Andreas Sebastian Klein, M. Sc. biochemist, scientific co-worker *28. August 1987 in, Germany Education since 04/2013 Doctoral studies in the group of Prof. Dr. Pietruszka Institute for Bioorganic Chemistry»Mutasynthesis meets synthetic biology«2012 Master thesis in the group of Prof. Dr. Seidel Institute of Molecular Physical Chemistry (MPC) 2010 Master studies in biochemistry at the Heinrich Heine University 2010 Bachelor thesis in the group of Prof. Dr. Pohl Biocatalysis & Biosensors (IBG-1) at Forschungszentrum Jülich 2007 Bachelor studies in biochemistry at the Heinrich Heine University 2007 Abitur, Dietrich-Bonhoeffer Gymnasium Ratingen Scholarships since 04/2013 Scholarship of the CLIB Graduate Cluster Industrial Biotechnology Doctoral Speaker of the CLIB Graduate Cluster Organizer of the 5 th CLIB Graduate Cluster & igraspseed Symposium»From Gene to Protein and Beyond«, 18. September 2014, Publications A. S. Klein, A. Domröse, P. Bongen, H. U. C. Brass, T. Classen, A. Loeschcke, T. Drepper, L. Laraia, S. Sievers, K.-E. Jaeger, J. Pietruszka, ACS Synth. Biol. 2017;»New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida«a. S. Klein, A. Domröse, J. Hage-Hülsmann, S. Thies, V. Svensson, T. Classen, J. Pietruszka, K.-E. Jaeger, T. Drepper, A. Loeschcke, Frontiers in Microbiology 2015, 6;»Efficient recombinant production of prodigiosin in Pseudomonas putida«page 1 of 5
2 Poster/ Talks Directing Biosynthesis V, March 2017, Warwick, United Kingdom; H. U. C. Brass, A. S. Klein, T. Classen, A. Domröse, A. Loeschcke, T. Drepper, K.-E. Jaeger, J. Pietruszka,»New Prodigiosin Derivatives A mutasynthesis approach«(poster & Short-Talk) University of Cambridge, 26. September 2016, Cambridge, United Kingdom; A. S. Klein,»Creating Diversity in Prodiginines Chemistry Meets Synthetic Biology«(Talk)»Future Bioeconomy«PhD Day NRW, 22. September 2016,, Germany; A. S. Klein, A. Domröse, A. Loeschcke, T. Classen, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Creating diversity in prodiginines Biotechnology meets chemistry«(poster + Talk) Gordon Research Conferences Natural Products & Bioactive Compounds, 31. July 05. August 2016, Andover (NH), USA; A. S. Klein, A. Domröse, A. Loeschcke, T. Classen, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Creating diversity in prodiginines A mutasynthesis approach«(poster) Expert for Bioeconomy: Berufsfindungspraktikum»Biologie-Chemie«, 11. July 2016, Forschungszentrum Jülich, Jülich, Germany; A. S. Klein,»Bioeconomy«(Talk) IV International Conference on Antimicrobial Research (ICAR), 29. June 01. July 2016, Torremolinos, Spain; J. Hage-Hülsmann, A. Grünberger, S. Thies, A. Domröse, A. S. Klein, D. Binder, F. Hilgers, D. Kohlheyer, J. Pietruszka, K.-E. Jaeger, T. Drepper, A. Loeschcke,»Combinatorial antibiotic effects of natural biocide cocktails«(co-author: Poster) S. Thies, A. Loeschcke, A. Domröse, A. S. Klein, J. Pietruszka, F. Hilgers, R. Weihmann, D. Binder, K. Troost, K.-E. Jaeger, T. Drepper,»Novel microbial production strategies for natural products with antimicrobial properties«(co-author: Poster) Annual Conference of the Association for General and Applied Microbiology (VAAM), March 2016, Jena, Germany; A. Domröse, R. Weihmann, A. S. Klein, J. Pietruszka, K.-E. Jaeger, T. Drepper, A. Loeschcke,»yTREX Update of a synthetic biology tool for effective gene cluster cloning and secondary metabolite production«(co-author: Poster) 7 th CLIB Graduate Cluster Symposium»From Gene to Protein and Beyond«, 08. April 2016,, Germany; A. S. Klein,»Creating diversity in prodiginines A bridge between chemistry and biology«(talk) 28. Irseer Naturstofftage, February 2016, Irsee, Germany; A. S. Klein, A. Domröse, A. Loeschcke, T. Classen, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Chemo-enzymatic Production of Prodiginines«(Poster + Short-Talk) 6 th European Conference on Prokaryotic and Fungal Genomics (ProkaGENOMICS), 29. September 02. October 2015, Göttingen, Germany; A. Domröse, A. S. Klein, J. Pietruszka, K.-E. Jaeger, T. Drepper, A. Loeschcke,»TREX A synthetic biology tool for the bacterial production of prodigiosin«(co-author: Poster) page 2 of 5
3 IV. Symposium of the Interdisciplinary Graduate School of Natural Products Research (Saarland University), 05. August 2015, Saarbrücken, Germany; A. S. Klein, A. Domröse, A. Loeschcke, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Chemo-enzymatic production of the Antibiotic Prodigiosin in Pseudomonas putida«(poster + Talk) The 6 th Congress of European Microbiologists (FEMS), June 2015, Maastricht, Netherlands; A. Loeschcke, S. Thies, J. Hage-Hülsmann, A. Grünberger, D. Binder, A. Domröse, A. S. Klein, D. Kohlheyer, J. Pietruszka, K.-E. Jaeger, T. Drepper,»Heterologous production of antibacterial secondary metabolites from Serratia marcescens in Pseudomonas putida«(co-author: Poster + Talk) Directing Biosynthesis IV, March 2015, Norwich, United Kingdom; A. S. Klein, A. Domröse, A. Loeschcke, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Chemo-enzymatic production of the antibiotic prodigiosin in Pseudomonas putida«(poster) Synthetic biology of antibiotic production II, 30. August 04. September 2014, Sant Feliu de Guíxols, Spain; A. S. Klein, A. Domröse, A. Loeschcke, K.-E. Jaeger, T. Drepper, J. Pietruszka,»Chemoenzymatic production of the antibiotic prodigiosin«(poster) 10 th NCCR Symposium on Trends in Structural Biology, August 2012, Zurich, Switzerland; S. Kalinin, T. Peulen, S. Sindbert, P. J. Rothwell, S. Berger, T. Restle, R. S. Goody, H. Gohlke, V. Keitel, L. Spomer, D. Häussinger, M. Frohnapfel, Q. Ma, A. S. Klein, C. A. M. Seidel,»In vitro and in vivo FRET restrained high-precision structural modeling of proteins and their complexes«(poster) Conferences Attendances (without contribution) 20. Tag der organischen Chemie (TOCUS) / 20 th»day of organic Chemistry«, 14. October 2016, University of Stuttgart, Stuttgart, Germany 1 st European Conference on Natural Products, September 2013, Frankfurt a. M., Germany 3 rd P-CUBE User Meeting, 22. October 2012, EMBL, Heidelberg, Germany Special Training/ Workshop Attendances Fortbildungsveranstaltung nach 15 Abs. 2 Nr.3, Abs. 4 Gentechnik- Sicherheitsverordnung (GenTSV), AGCT Advo Gen Consulting Bender und Kauch GbR, March 2014,, Germany Lab Course»PolyOmics High Throughput Sequencing«, August 2014, CeBiTec, University of Bielefeld, Germany Writing Workshop»Research paper«, July 2014, Interdisciplinary Graduate and Research Academy (igrad),, Germany Introduction to Project- & Innovation Management and Patent Law, March 2014, CLIB Graduate Cluster Industrial Biotechnology, Wuppertal, Germany page 3 of 5
4 VAAM Workshop»Biology of Bacteria Producing Natural Products«, September 2013, Frankfurt a. M., Germany Workshop on Advanced Protein Engineering Techniques, October 2012, EMBL, Heidelberg, Germany Teaching Natural Product Synthesis (Teaching Fellow) 2017, Heinrich Heine University, Enzyme Technology II (Teaching Fellow) 2016, Heinrich Heine University, Bioeconomy Academy (Coordinator & Teaching Fellow) 2015, Heinrich Heine University, - Experiment Designer CLIB-GC Lab Course»In vitro Evolution, Expression and Biocatalysis«(Teaching Fellow) 2015, Heinrich Heine University, - Seminar»Enzyme kinetics«enzyme Technology II (Coordinator & Teaching Fellow) 2015, Heinrich Heine University, - Lecture»Chemical Screening«CLIB-GC Lab Course»In vitro Evolution, Expression and Biocatalysis«(Coordinator & Teaching Fellow) 2014, Heinrich Heine University, - Seminar»Enzyme kinetics«enzyme Technology II (Coordinator & Teaching Fellow) 2014, Heinrich Heine University, Enzyme Technology I (Teaching Fellow) 2014, Heinrich Heine University, Natural Product Synthesis (Teaching Fellow) 2013, Heinrich Heine University, Enzyme Technology II (Teaching Fellow) 2013, Heinrich Heine University, page 4 of 5
5 CLIB Graduate Cluster Industrial Biotechnology 6 th CLIB Graduate Cluster & igraspseed Symposium»From Gene to Protein and Beyond«, 20. August 2015,, Germany 5 th CLIB Graduate Cluster & igraspseed Symposium»From Gene to Protein and Beyond«, 18. September 2014,, Germany 4 th CLIB Graduate Cluster & igraspseed Symposium»From Gene to Protein and Beyond«, 18. September 2013,, Germany 7 th Annual CLIB-GC Retreat, February 2016, Herdecke, Germany; A. S. Klein, A. Domröse, A. Loeschcke, T. Classen, T. Drepper, K.-E. Jaeger, J. Pietruszka,»Mutasynthesis meets synthetic biology - Production of Prodiginines«(Poster) 6 th Annual CLIB-GC Retreat, March 2015, Wermelskirchen, Germany; A. S. Klein, J. Pietruszka,»Mutasynthesis approach for prodiginine derivatives«(poster + Talk) 3 rd Internal CLIB-GC Retreat, October 2014, Mülheim a. d. Ruhr, Germany; A. S. Klein,»Mutasynthesis approach for prodiginine derivatives«(talk) 5 th Annual CLIB-GC Retreat, February 2014, Lünen, Germany; A. S. Klein, J. Pietruszka,»Mutasynthesis approach for prodiginine derivatives«(poster) 2 nd Internal CLIB-GC Retreat, October 2013, Kerkrade, Netherlands; A. S. Klein,»Mutasynthesis approach for prodiginine derivatives«(talk) page 5 of 5
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