MAIN RESULTS OBTAINED

Transcription

1 BSDP FEEDING KNOWLEDGE - Expo 2015

2 EXPERIMENTAL ACTIVITIES The project development has involved a wide scope of testing activities, on field and post harvest, to assess the validity of ozone and electrolized water against several diseases and microbial activity. ToTal TesTs conducted: 46 disease TreaTed: cultivations TesTed: Brown spot of pear pseudomonas syringae disease Valsa canker white haze Brown rot on peach and apricot Bacterial canker of peach peach leaf curl rice Blast armillaria root disease metschnikowia esca disease gray mould late Blight powdery mildew mould / yeast european canker on apple and pear fire Blight apple and pear scab actinidia (kiwi) apple apricot Berries (other) celery cherry grape lemon lettuce mango melon orange peach pear plum prickly pear pumpkin radicchio rice sprout strawberry tomato zucchini

3 POST HARVEST OZONE TREATMENT On strawberries treated with ozone, preservation time can increase and even double: treated fruits do show higher quality and no decay after 10 days, while untreated fruits show decomposition traces already after 5 days. Microbial load is also lower after ozone treatment. treatment with ozone for 24h at 5 ppm product Before treatment and storage cfu/g cell storage with ozone 0 yeast Bacteria fungi T=2 no treatment after 5 days after 10 days cfu/g cell storage cfu: Colony-Forming Units test developed with: Institute for Sustainable Plant Protection, National Research Council - CNR official (CNR IPP) and IAMB International Centre for Advanced Mediterranean Agronomic Studies/ Mediterranean Agronomic Institute of Bari (IAMB)

4 POST HARVEST OZONE TREATMENT When treated with ozone, strawberry preservation time can increase significantly also in absence of cold room storage. after 5 days preservation with continuous ozone at 0.1 ppm T=20 preservation with air test developed with: Institute for Sustainable Plant Protection, National Research Council - CNR official (CNR IPP) and IAMB International Centre for Advanced Mediterranean Agronomic Studies/ Mediterranean Agronomic Institute of Bari (IAMB)

5 POST HARVEST OZONE TREATMENT Apples that were previously preserved in ozone atmosphere register a shelf-life double than apples preserved in standard cells. Damages appear after 1 week on standard cell apples, after 2 weeks on ozone treated apples. Mycobacterial load data are aligned with these measurements. apple granny smith tanken on after preservation in ozone cell 36 ufc/g apple granny smith tanken on after preservation in standard cell 870 ufc/g 1 week in air 1 week in air T=+18 T=+18 test developed by cristian carboni, de nora next

6 POST HARVEST OZONE TREATMENT Kiwi preserved with ozone show a 4% decrease of losses compared to standard preservation techniques. After 13 days, the pulp is more solid and colour is more uniform: ozone blocks the development of mycelia and spores. product Before treatment and storage (1 stock with low fungi, 1 stock with high fungi-botrytis) preservation in ozone cell after 3.5 months spores only in contact zones sample previously preserved with ozone 5 points (red) mycelia 29 points (blue) mold 13 days in air T=+18 sample previously preserved in standard cell preservation in cell with ethylene absorber (permanganate) widely distributed spores test developed by cristian carboni, de nora next 21 points (red) mycelia 41 points (blue) mold

7 PROCESS WATER REUSE OZONE PROCESS WITH RECYCLING AND OZONE Input water: 40 l/min From tank 2, a water quantity of 40 l/min is collected, treated with ozone and transfered to tank 1. TANK 1 (2 m 3 ) OZONISER Filter TANK 2 (3.5 m 3 ) PACKAGING TRADITIONAL PROCESS Waste water drain 40 l/min Input water: 40 l/min Input water: 40 l/min WATER SAVING: +50% Tank 1 and tank 2 receive a water quantity of 40 l/min Filter TANK 1 (2 m 3 ) TANK 2 (3.5 m 3 ) Waste water drain 40 l/min Waste water drain 40 l/min

8 SPECIALIZED TESTING AREAS italy (in progress) nursery

9 The application of electrolyzed water solution at 300 ppm has allowed to stop fire blight infection and its diffusion in the pear orchard affected (Abate Fétel): only when treated with electrolyzed water the infection was permanently removed. treatment dates: electrolyzed water and cupravit: 16/4, 30/4, 08/05, 14/05, 21/5, 29/5 Bion: 9/4, 16/4, 30/4, 08/05, 14/05, 21/ /05/2014 Electrolyzed water 300 ppm Untreated 7 number of infected branches / plots /08/2014 NO INFECTION APPEARANCE 1 0 untreated electrolyzed water 300 ppm cupravit wg 200 g/l Bion 50 wg 200 g/ha Pear (Abate Fètel) Reggio Emilia, Italy Test conducted by SPF GAB Trial Center

10 Untreated Pear (Abate Fètel) Reggio Emilia, Italy Test conducted by SPF GAB Trial Center

11 BEFORE TREATMENT AFTER TREATMENT Pear (Juaco, Spain) Test conducted with FRUTTUR collaboration

12 The application of electrolyzed water solution at 400 ppm on apples (Modì) affected by White Haze (pathogen Tilletiopsis spp) has resulted in an increase of wholesome product percentage from 61% to 87%. % of apples Modì without white patina (white stains_tilletiopsis spp) 100,0 90,0 80,0 86,6 electrolyzed water pesticide usually employed by farmer 70,0 60,0 61,5 no infection low infection (stains <20% on fruit surface) 50,0 40,0 30,0 20,0 10,0 0 No infection medium infection (20% < stains <50% on fruit surface) high infection (stains >50% on fruit surface) APPLE Modì Weekly treatments from June to end-august 2010 Trials on 500 fruits Replication: 4 (6-tree plots) Trial type: classification of apples based on surface extension of WH (White Haze)

13 Pre-harvest application of electrolyzed water solution at 400 ppm on apples (Pink Lady) has resulted in a 10% to 20% increase of wholesome product after 4 months of preservation, with a 7-10% reduction of decomposition and infection with fungi (gloesporium). POST-PRESERVATION TRIAL ON APPLES (PINK LADY ROSY GLOW) 100 electrolyzed water 90 pesticide usually employed by farmer ,5 49 protocol Thesis A: treatment with electrolyzed water on 28/08/2010; 04/09/2010; 15/09/2010; 24/09/2010; 22/10/2010; 06/11/2010 dati % ,5 21,5 17,5 14 wholesome gloesporium other decompositions / losses Thesis T: treatment with pesticide usually employed by farmer (from May to 01/10/2012 Captan; 18/10/2010 Bellis) - harvest 18/11/ sampling: 10 crates. 10x20=200 fruits for each thesis. - preservation in dynamic control atmosphere (dca) (O2:0,5-0,7 %; CO2: 0,7-0,8 %; T=1,8-2 C ; relative humidity: 90-92%) - sampling 14/03/2011 APPLE PINK Trials on 200 fruits for each thesis.

14 POST-PRESERVATION TRIAL ON APPLES (PINK LADY ROSY GLOW) electrolyzed water pesticide usually employed by farmer protocol dati % ,02 87,15 Thesis treated with electrolyzed water: treatments from end-june every 7-10 days (400 ppm) Thesis with pesticide usually employed by farmer: Bellis wholesome 0,85 gloesporium 11,84 harvest 30/10/2010 preservation in cell at controlled atmosphere. sampling 16/03/2011 SAMPLE TREATED WITH ELECTROLYZED WATER SAMPLE TREATED WITH PESTICIDE APPLE (FERRARA, ITALy) Test conducted with Fondazione F.lli Navarra per l Agricoltura collaboration

15 Treatment with electrolyzed water allow the reduction of residuals and plants are in healthy phytomedical state APPLE - PINK LADY 2011 PEAR - ABATE FÉTEL THESIS - Pesticide usually employed by farmer - Treated ONLY with electrolyzed water from half-july 2011 (suspension of all other fungicides) survey: multiresidual on 20 fruits THESIS - Pesticide usually employed by farmer - Treated from spring (pear scab: iprodione and electrolyzed water) survey: multiresidual on 20 fruits Pesticide ELECTROLYZED WATER Pesticide ELECTROLYZED WATER RESIDUALS 4 1 active principle quantity mg/kg quantity mg/kg Dithianon Boscalid Pyraclostrobin Captan RESIDUALS 5 2 active principle quantity mg/kg quantity mg/kg Iprodione Dithiocarbamate Tetraconazole Captan Kresoxim methyl 0.006