Plant systems biology approach to study the influence of environmental constraints on the production of plant-sourced polymers.
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1 Plant systems biology approach to study the influence of environmental constraints on the production of plant-sourced polymers
2 Public Research Centre Gabriel Lippmann (Luxembourg) 200 researchers and technicians 3 major Departments Material science Computer science Environment and Agro-biotechnologies (EvA) In 1908, G. Lippmann received the Nobel Prize for Physics for having Département elaborated his undulatory wave theory of 2 light, from which the interferential photograph is the practical result Department Environnement Environment and et Agro-biotechnologies
3 Plant systems biology approach of mineral nutrition for bio-sourced polymers production in plants Higher demand Food quantity and quality Renewable energy Biosourced materials Reduced ressources Climate change Arable land and nutrients Fossil energy Equilibrium disruption Need to cope with changes Bio-based economy Plants for tomorrow Plant photosynthesis is still the most efficient technology to convert sunlight to biosourced polymers, food or energy
4 Plant systems biology approach of mineral nutrition for bio-sourced polymers production in plants General Objective systems biology description of the mechanisms governing the influence of mineral nutrition on synthesis of biopolymers in planta 1- Develop a systems biology approach 2- Study the impact of mineral nutrition on production and quality of fibres in alfalfa Specific Objectives 3- Assess the matching of characteristics of the fibers produced with those demanded for industrial applications 4- Method development and/or optimization to perform the analyzes
5 Plant systems biology approach of mineral nutrition for bio-sourced polymers production in plants Metabolites Metabolomics Proteins Proteomics RNA Transcriptomics Orbitrap UPLC, HPLC LC-MS, LC-MS/MS ICP-MS Gel proteomics LC proteomics Maldi TOFTOF Orbitrap TOFTOF Microarray Q-PCR RNA-sec Imaging: Orbitrap MSMS Maldi TOFTOF Confocal Microscopy NanoSIMS50 Imaging GFP and confocal microscopy Maldi TOFTOF Antiobodies Imaging FISH and confocal microscopy Integrative biology approach
6 Plant systems biology approach of mineral nutrition for bio-sourced polymers production in plants Analytical chemistry platform Sample preparation: Accelerated solvent extraction (ASE), online and offline Solid-Phase Extraction (SPE), Solid-Phase MicroExtraction (SPME), freeze drying, microwave mineralisation, Automated Liquid handling workstation Chromatography: Ion-exchange chromatography (conductivity, amperometry), Liquid chromatography (UPLC, HPLC), Gas Chromatography Mass Spectrometry (GC-MS, GC-MSMS), Liquid Chromatography Mass Spectrometry (LC-MS, LC-MS/MS) Multi-Element analysis: Inductively coupled plasma Mass Spectrometry (ICP-MS), Direct Mercury analyser, TOC/TN, CHNS analyser
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8 Material analysis platform Imaging at the cellular scale Cameca NanoSIMS 50 Lateral resolution (X,Y) : 50 nm (M - ) Mesophile Leaf 40 Ca 64 Zn Epidermis Detection limits : ppm to 100 % Elemental range : H to U, isotopes Major use : imaging Simultaneous detection of 5 ionic species 10 µm Na Wood Mg Root 15 N/ 14 N 3, ± 3, N/ 14 N 3, ± 6, Mapping and elemental composition
9 Plant systems biology approach of mineral nutrition for bio-sourced polymers production in plants Thank you for your attention
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