Electrophoresis Systems

Transcription

1 Electrophoresis Systems Horizontal Gel Systems Vertical Slab Gel Systems 2-D Electrophoresis Systems Blotting Systems Power Supplies Gel Drying Gel Documentation Gel Cutting Tips Transilluminators Electrophoresis Reagents

2 Horizontal Gel Systems clearly better resolution. Injection Moulded Construction durable, leak-proof environment for complete safety and long life Cassette Type Electrodes inexpensive, easy to replace. Made of 99.99% corrosion resistant, pure platinum Electrical Safety lid can be located in one way only. On removal, power is disconnected from the buffer chamber Multiple Gel Trays eliminate the need for additional gel tanks Easy Click Lid Removal Casette Type Electrodes can be easily replaced by the user Plug and go gel casting Casting dams allow gels to be rapidly cast externally while the Clarit-E unit is in use for gel running. Flexicasters allow the casting of varying gel lengths simply by locking the moveable dam. Adjustable feet and level bubble allow gels of uniform thickness to be poured. Trays are UV transparent. White PVC platform cover and red loading guides can be optionally applied to enhance well visibility. Gel trays are free of indentations or casting gate channels, which can interfere with sample progression. UV transparent Gel Scoops, available for each unit size, offer a safe and easy way to transport gels to a UV Transilluminator. 2

3 The Clarit-E series of horizontal gel units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. All units offer unsurpassed economy of gel and buffer volume, with gel size and sample number versatility. This eliminates the need for multiple gel tanks for changes in gel size or application. Corrosion resistant gold plated terminals Retractable connectors compatible with most major power supplies. All units contain removable UV transparent trays. Gel size and sample number requirements can be exactly matched in each unit as systems are available with one, two or three tray options (dependent on model). Easy to use, leak proof plug and go gel casting dams are included as standard to allow gels to be rapidly cast whilst the Clarit-E unit is in use for gel running. Traditional tape casting can be used, should this be preferred. Although lid connectors are compatible with most major power supplies, adapters are available to provide complete compatibility. High efficiency horizontal gel cooling Removable gel table allows a Coolpack to sit above the gel in buffer offering enhanced gel resolution and faster run times. Extensive range of combs Four thicknesses, colour coded: Black: 0.75mm for ultra resolved bands White: 1mm supplied as standard Red: 1.5mm for maximising sample volume Blue: 2mm for maximising sample volume Options for sample prep Options for multichannel pipette loading The number of samples can be maximised using combs with higher numbers of teeth. All units are CE marked and are in conformity with the relevant CE Safety Directives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC :2001. EN :2001; 89/336 /EEC EMC DIRECTIVE: EN55011 Emissions, EN Immunity. 3

4 Mini Horizontal Gel Tank Midi Horizontal Gel Tank Available with 7 x 7cm, 7 x 10cm or with both gel trays Economic low gel and buffer volumes Small lab bench footprint Typical application: Designed for quick checks of low to medium numbers of samples. The Clarit-E Mini is the smallest unit in the range, designed to maximise run economy without compromising versatility as two tray options are available, and combs ranging from preparative up to 16 samples. Maximum separation distance is 9cm. Gel dimensions (W x L) 7 x 7cm, 7 x 10cm Unit dimensions (W x L x H) 21 x 9 x 9cm Max. sample: 7 x 7cm tray 32 samples 7 x 10cm tray 64 samples Buffer volume 225ml Combs available: No. of samples 1, 2, 4, 8MC, 8, 10, 12MC, 16 Thickness 0.75, 1, 1.5, 2mm Available with 10 x 7cm, 10 x 10cm or with both gel trays Run up to 100 samples Low buffer volumes Ideal for rapid electrophoresis Typical application: Ideal for quick checks of samples from PCR and cloning. Designed for routine checks of a medium number of samples over short to medium gel lengths. Wider than the Clarit-E Mini it allows more samples to be resolved per gel without a significant increase in buffer or gel volumes. Gel dimensions (W x L) 10 x 7cm, 10 x 10cm Unit dimensions (W x L x H) 22 x 12.5 x 9cm Max. sample: 10 x 7cm tray 50 samples 10 x 10cm tray 100 samples Buffer volume 300ml Combs available: No. of samples 1, 2, 4, 8, 10MC, 12, 16, 20MC, 25 Thickness 0.75, 1, 1.5, 2mm Mini Horizontal Gel Tank Midi Horizontal Gel Tank Catalogue EL1000 EL1100 EL1050 EL1002 EL1102 Clarit-E Mini, 7 x 7cm UV tray, 2 x 8 sample combs, loading guides & dams Clarit-E Mini, 7 x 10cm UV tray, 2 x 8 sample combs, loading guides & dams Clarit-E Mini, 7 x 7cm & 7 x 10cm UV tray, 2 x 8 sample combs, loading guides & dams 7 x 7cm UV tray 7 x 10cm UV tray Catalogue EL1200 EL1300 EL1250 EL1202 EL1302 Clarit-E Midi, 10 x 7cm UV tray, 2 x 16 sample combs, loading guides & dams Clarit-E Midi, 10 x 10cm UV tray, 2 x 16 sample combs, loading guides & dams Clarit-E Midi, 10 x 7cm & 10 x 10cm UV tray, 2 x 16 sample combs, loading guides & dams 10 x 7cm UV tray 10 x 10cm UV tray Components of Clarit-E EL1050 Buffer saver blocks are available for all units, which physically reduce the volume of a gel chamber and so reduce buffer requirements, saving cost. Loading Guides allow easy well identification and sample loading. Scoops for safe transfer of gels. 4

5 Midi 96 Horizontal Gel Tank MultiSUB4 The Clarit-E Midi 96 is ideal for separation of PCR products, or DNA samples containing only a few bands, from 96- well microtitre plates and thermal cycler blocks. Rapid Testing 96 wells can be cast simultaneously within a single gel in a precise 96-well microplate configuration. Rapid Loading gels can be loaded quickly and easily by 8-channel pipette. Extended Separation offset layout of the wells ensures a longer run length per well. High Speed & High Throughput 96 samples loaded and separated in 15 to 30 minutes without loss of resolution. Easy Sample Identification exact configuration of 96- well microplates allows samples to be quickly and easily identified from original microplates. Gel dimensions (W x L) 10 x 12cm Unit dimensions (W x L x H) 22 x 12.5 x 9cm Max. sample capacity: 96 samples plus 1 or 2 marker lanes Buffer volume 300ml Combs available: No. of samples 8MC + 1 marker lane 8MC + 2 marker lanes Thickness: 1.5, 2mm Recommended power supply EL2720 Midi 96 Horizontal Gel Tank EL1350 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs with 1 marker lane, casting dams EL1355 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs with 1 marker lane, casting dams EL1360 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs with 2 marker lanes, casting dams EL1365 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs with 2 marker lanes, casting dams Accessories and Combs EL1370 Comb block for EL1350 EL1375 Comb block for EL1355 EL1380 Comb block for EL1360 EL1385 Comb block for EL1365 EL sample, 1mm thick comb, 1 marker lane EL sample, 1.5mm thick comb, 1 marker lane EL sample, 1.5mm thick comb, 2 marker lanes EL sample, 1mm thick comb, 2 marker lanes EL1390 Clarit-E Midi, 96 well tray The Clarit-E MultiSUB 4 combines high throughput electrophoresis with the space and time-saving advantages of a small, compact unit. High Throughput a maximum 1200 samples may be separated in as little as 15 minutes in 4 vertically stacked gel trays. Rapid Loading multichannel-compatible combs and gel plate configurations compatible with microplates and thermal cycler blocks ensure rapid loading of DNA minipreps and PCR products by 8-channel pipette. Thicker Combs double-sided 1.5mm thick combs allow more sample volume to be loaded into each well. 3 Gel tray Options available in 8 x 6, 8 x 12 and 8 x 18cm (W x L) sizes for maximum flexibility. High Speed a compact gel tank maximises the intensity of the electric field for rapid separations. Small Footprint helps maximise bench space. Gel dimensions, (W x L) 8 x 6cm, 8 x 12cm, 8 x 18cm Unit dimensions (W x L x H) 28 x 9 x 9cm Max. sample capacity 1200 Buffer volume 1800ml Combs available : No. of samples 12/1 sample and 18/8 sample Thicknesses 1.5mm Recommended power supply EL2720 MultiSUB4 EL1980 Clarit-E multisub4, multi-level gel chamber, includes 4 x 8 x 12cm UV trays, 4 x 18/8-sample, 1.5mm thick combs Accessories and Combs Catalogue EL1982 EL1984 EL1986 EL /1-1.5 EL /8-1.5 EL x 6cm UV tray 8 x 12cm UV tray 8 x 18cm UV tray multisub4 12/1-sample, 1.5mm thick comb multisub4 18/8-sample, 1.5mm thick comb External caster for multisub4 5

6 Choice Maxi Three tray options Run up to 210 samples Low buffer volumes Multichannel pipette compatible combs for speed loading Typical application: Ideal for restriction fragment analysis, sample prep or checking of high numbers of samples. The Clarit-E Choice offers a wide degree of versatility. Three tray options are available allowing the choice of one, two or all three gel length options at the time of purchase. The 15cm total run length allows restriction fragment or other close MW sample bands to be easily separated and identified. Gel dimensions, (W x L) 15 x 7cm, 15 x 10cm, 15 x 15cm Unit dimensions (W x L x H) 26.5 x 17.5 x 9cm Max. sample: 15 x 7cm tray 70 samples 15 x 10cm tray 140 samples 15 x 15cm tray 210 samples Buffer volume 500ml Combs available : No. of samples 1, 2, 4, 10, 10MC, 12, 14MC, 16MC, 18MC, 20, 28MC, 30MC, 35 Thicknesses 0.75, 1, 1.5, 2mm Available with 20 x 25cm, 20 x 20cm or 20 x 10cm gel trays Run up to 550 samples Low buffer volumes Ideal for extended separations Typical application: Primarily designed for separating high numbers of samples from PCR or cloning. The Clarit-E Maxi allows ultra high-resolution separations over extended runs. Tray sizes correspond to standard blotter sizes. It also allows easy sample transfer onto a membrane for further analysis. Multichannel pipette compatible combs facilitate speed loading. Gel dimensions, (W x L) 20 x 10cm, 20 x 20cm, 20 x 25cm Unit dimensions (W x L x H) 39.5 x 23 x 9cm Max. sample: 20 x 10cm tray 200 samples 20 x 20cm tray 450 samples 20 x 25cm tray 550 samples Buffer volume 1200ml Combs available: No. of samples 1, 2, 4, 10, 16, 20MC, 25, 30, 36, 40MC, 50 Thicknesses 0.75, 1, 1.5, 2mm Choice Maxi Catalogue EL1450 EL1400 EL1500 EL1550 Clarit-E Choice, 15 x 7cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Choice, 15 x 10cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Choice, 15 x 15cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Choice Trio, 15 x 7, 10 & 15cm UV tray, 2 x 20 sample combs, loading guides and dams Catalogue EL1600 EL1620 EL1640 EL1660 Clarit-E Maxi, 20 x 25cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Maxi, 20 x 20cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Maxi, 20 x 10cm UV tray, 2 x 20 sample combs, loading guides & dams Clarit-E Maxi, 20 x 20cm & 20 x 10cm UV tray, 2 x 20 sample combs, loading guides and dams Accessories Accessories Catalogue EL1452 EL1402 EL x 7cm UV tray 15 x 10cm UV tray 15 x 15cm UV tray Clarit-E Choice Trio includes all 3 tray sizes for optimum versatility and value. Catalogue EL1642 EL1622 EL x 10cm UV tray 20 x 20cm UV tray 20 x 25cm UV tray Casting dams allow gels to be rapidly cast externally while the Clarit-E unit is in use for gel running. 6

7 Screen Fast Mini Available with 26 x 16, 26 x 24 and 26 x 32cm or all three gel trays Run up to 672 samples Low buffer volumes Multichannel pipette compatible combs for speed loading Typical application: Ideal for rapid screening of very large numbers of clonal or PCR samples or extended high resolution separations. The Clarit-E Screen allows loading and analysis of exactly seven 96 well format micro titre plates. The large gel run length of up to 32cm also allows resolution of samples over a long distance for separation of complex sample bands such as in restriction fragment analysis. Buffer recirculation ports are included to allow enhanced resolution over extended runs. Gel dimensions (W x L) 26 x 32cm, 26 x 24cm, 26 x 16cm Unit dimensions (L x W x H) 50 x 28 x 9cm Max sample: 26 x 32cm tray 672 samples 26 x 24cm tray 504 samples 26 x 16cm tray 336 samples Buffer volume 1400ml Combs available No. of samples 28MC, 56MC Thicknesses 0.75, 1, 1.5, 2mm Screen A convenient self-contained solution for routine agarose gel electrophoresis Ultra compact Low buffer and gel volumes Multichannel pipette compatible combs for speed loading Typical application: For routine, rapid checking of samples. This completely self contained system allows gel casting, running and analysis to be performed in the same unit. The parallel electrode arrangement allows ultra efficient current transfer, enabling resolution to be completed within 30 minutes. The UV transparent base allows direct viewing on a UV Transilluminator with no need for time-consuming transfer and potential gel damage. Dual comb slots allow the loading of up to 40 samples per gel. Gel dimensions, (W x L) Unit dimensions (W x L x H) Max. sample capacity Buffer volume Combs available: No. of samples Thicknesses 10 x 8cm 15 x 15 x 4cm 40 samples 50ml 1, 4, 8, 12, 16, 20MC 1, 1.5mm Catalogue EL1800 EL1820 EL1840 EL1860 Clarit-E Screen, 26 x 32cm UV tray, 6 x 28 sample combs, loading guides & dams Clarit-E Screen, 26 x 24cm UV tray, 6 x 28 sample combs, loading guides & dams Clarit-E Screen, 26 x 16cm UV tray, 6 x 28 sample combs, loading guides & dams Clarit-E Screen, 26 x 16, 24, & 32cm UV tray, 6 x 28 sample combs, loading guides and dams Fast Mini EL1700 Clarit-E Fast Mini, 10 x 8cm, 2 x 8 sample combs 1.5mm, and casting dams EL1702 Fast Mini casting dams, pk/2 Accessories Catalogue EL1802 EL1822 EL x 32cm UV tray 26 x 24cm UV tray 26 x 16cm UV tray All Clarit-E Screen combs are multichannel pipette compatible. 7

8 Accessories for Horizontal Units Presented here in an easy to read table, simply choose the gel tank of interest across the top, and the accessory required from the left hand side, then look across for the appropriate catalogue number. Electrode Casettes Casette type electrodes are inexpensive and easy to replace. Made of 99.99% pure corrosion resistant platinum. Casting Dams Casting dams allow gels to be rapidly cast externally whilst the gel tank is in use. Adhesive Loading Guides Allow easy well identification and sample loading. Viewing Platform The white PVC platform cover can be optionally applied to enhance well visibility. High Efficiency Horizontal Gel Cooling Effective cooling Cools without external chilling systems Improves gel resolution and reduces run times Comprising a removable Gel Platform and CoolPack this unique system will cool the buffer chamber of Clarit-E horizontal gel units without any external chilling. Simply place the platform over the running tray and place a pre-chilled CoolPack on top. This allows the CoolPack to sit above the gel in buffer directly removing any heat generated. Buffer Saver Blocks Buffer saver blocks are placed into the positive and negative compartments of a gel chamber and physically reduce the volume of buffer required, saving cost. Blocks are available for all Clarit-E Horizontal Gel Units. Flexi Casters Allows gels to be cast of various lengths simply by locking the moveable dam. Adjustable feet and bubble level ensure gels are of uniform thickness. Gel Scoops UV transparent gel scoops, available for each unit size, offer a safe and easy way to transport gels. Gel Levelling Table The gel levelling table provides a useful way of pouring gels of uniform thickness. The table features a 32 x 26cm platform with large thumbwheel levelling feet at each corner, as well as a levelling bubble. Accessories Mini Midi Choice Maxi Screen Positive electrode EL1006 EL1206 EL1406 EL1606 EL1808 Negative electrode EL1008 EL1208 EL1408 EL1608 EL1810 Casting Dams EL1004 EL1204 EL1404 EL1604 N/A Adhesive loading guides EL1010 EL1210 EL1410 EL1610 EL1804 Viewing platform EL1012 EL1212 EL1412 EL1612 EL1806 Cool pack and platform EL1014 EL1214 EL1414 EL1614 EL1812 Buffer saver blocks EL1016 EL1216 EL1416 EL1616 EL1814 Flexi Caster EL1018 EL1018 EL1418 EL1418 EL1816 Gel scoop EL1020 EL1220 EL1420 EL1624 EL1818 Gel Levelling Table EL1026 EL1026 EL1026 EL1026 EL1026 8

9 Combs for Horizontal Gel Systems Combi-Comb/Loading Guides Extensive range of combs Four thicknesses, colour coded: Black: 0.75mm for ultra resolved bands White: 1mm supplied as standard Red: 1.5mm for maximising sample volume Blue: 2mm for maximising sample volume Adjustable Height Options for sample preparation described as Prep Options for multi-channel pipette compatibility Look for MC in the description and catalogue number Simply add LG to the end of the product code for the comb you want. Colour coded for thickness Comb and guide in one piece for convenience Adjustable height These guides combine the convenience of a regular comb with the appropriate guide (same teeth number and width). For loading samples, simply flip over. The number of samples can be maximised using high tooth number combs. Clarit-E Mini Horizontal Gel Tanks EL1000, EL1050, EL1100 from page mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel Prep 1, Marker 1 EL µl EL µl EL µl EL µl Prep 2, Marker 2 EL µl EL µl EL µl EL µl Prep 4, Marker 2 EL µl EL µl EL µl EL µl 8 MC EL1000-8MC µl EL1000-8MC-1 11µl EL1000-8MC µl EL1000-8MC-2 23µl 8 EL µl EL µl EL µl EL µl 10 EL µl EL µl EL µl EL µl 12 MC EL MC µl EL MC-1 14µl EL MC µl EL MC-2 27µl 16 EL µl EL µl EL µl EL µl Clarit-E Midi Horizontal Gel Tanks EL1200, EL1250, EL1300, EL1350, EL1355, EL1360, EL1365 from pages 4 and mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel Prep 1, Marker 1 EL µl EL µl EL µl EL µl Prep 2, Marker 2 EL µl EL µl EL µl EL µl Prep 4, Marker 2 EL µl EL µl EL µl EL µl 8 EL µl EL µl EL µl EL µl 10 MC EL MC µll EL MC-1 27µl EL MC µl EL MC-2 54µl 12 EL µl EL µl EL µl EL µl 16 EL µl EL µl EL µl EL µl 20 MC EL MC µl EL MC-1 14µl EL MC µl EL MC-2 27µl 25 EL µl EL µl EL µl EL µl 9

10 Clarit-E Choice Horizontal Gel Tanks EL1400, EL1450, EL1500, EL1550 from page mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel Prep 1, Marker 1 EL µl EL µl EL µl EL µl Prep 2, Marker 2 EL µl EL µl EL µl EL µl Prep 4, Marker 2 EL µl EL µl EL µl EL µl 10 EL µl EL µl EL µl EL µl 12 EL µl EL µl EL µl EL µl 20 EL µl EL µl EL µl EL µl 35 EL µl EL µl EL µl EL µl 10 MC EL MC µl EL MC-1 29µl EL MC µl EL MC-2 59µl 14 MC EL MC µl EL MC-1 29µl EL MC µl EL MC-2 59µl 16 MC EL MC µl EL MC-1 27µl EL MC µl EL MC-2 54µl 18 MC EL MC µl EL MC-1 11µl EL MC µl EL MC-2 23µl 28 MC EL MC µl EL MC-1 11µl EL MC µl EL MC-2 23µl 30 MC EL MC µl EL MC-1 13µl EL MC µl EL MC-2 25µl Clarit-E Maxi Horizontal Gel tanks EL1600, EL1620, EL1640, EL1660 from page mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel Prep 1, Marker 1 EL µl EL µl EL µl EL µl Prep 2, Marker 2 EL µl EL µl EL µl EL µl Prep 4, Marker 2 EL µl EL µl EL µl EL µl 10 EL µl EL µl EL µl EL µl 16 EL µl EL µl EL µl EL µl 20 MC EL MC µl EL MC-1 27µl EL MC µl EL MC-2 54µl 25 EL µl EL µl EL µl EL µl 30 EL µl EL µl EL µl EL µl 36 EL µl EL µl EL µl EL µl 40 MC EL MC µl EL MC-1 11µl EL MC µl EL MC-2 23µl 50 EL µl EL µl EL µl EL µl Clarit-E Screen Horizontal Gel Tank EL1800, EL1820, EL1840, EL1860 from page mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel 28 MC EL MC µl EL MC-1 34µl EL MC µl EL MC-2 68µl 56 MC EL MC µl EL MC-1 14µl EL MC µl EL MC-2 27µl Clarit-E Fast Mini horizontal Gel tank EL1700 from page 7 1.0mm thickness 1.5mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Volume Volume 5mm 5mm thick gel thick gel 1, 1 marker EL µl EL µl 4 EL µl EL µl 8 EL µl EL µl 12 EL µl EL µl 16 EL µl EL µl 20 MC EL MC-1 10µl EL MC µl 10

11 Comet Assay Tanks For Single Cell Gel Electrophoresis Minimise exposure to light and reduce background DNA damage High efficiency cooling for enhanced resolution The Clarit-E COMET assay tanks are specialised electrophoresis units for Single Cell Gel Electrophoresis. The units are specifically designed to minimise exposure to light and reduce background DNA damage. Previously, units designed for standard electrophoresis have been used which with their clear construction have not offered an easy to use system. Constructed from Ebony acrylic to prevent UV degradation, the slides sit on a cooling platform which can be connected to a water supply or chiller for active temperature control and cooling. Comet Assay Tanks EL1900 Clarit-E Comet assay tank for 10 slides EL1920 Clarit-E Comet assay tank for 20 slides EL1940 Clarit-E Comet assay tank for 40 slides EL1960 Clarit-E Comet assay tank for 80 slides Cellulose Acetate Gels a complete range of wet cellulose acetate gels, which can be used for a wide variety of clinical electrophoresis applications. CellasGEL cellulose acetate gels are available in a variety of sizes and applications:- 2.5 x 14cm, 2.5 x 17cm, 5.7 x 14cm, 5.7 x 17cm, 5 x 24cm, 17 x 17cm, 6 x 7.6cm Helena, 5.7 x 10.2 arched. Kits are available for multiple tests on serum proteins, haemoglobin, urine proteins and for immunofixation. Applicators a variety of rapid sample applicators for a selection of applications: Two specimen applicator for semi micro samples on strips 2.5cm wide Four specimen applicator for semi micro samples on strips 5.7cm wide Four specimen applicator for haemoglobin samples on strips 5.7cm wide Six specimen applicator for samples on strips 5.7cm wide Bridges are available in a variety of formats for cellulose acetate sheets and for arched and horizontal Helena types of gels. Densitometer a universal densitometer scanner and software, able to read 144 tests per minute. Cellulose Acetate System Compact High Resolution Systems for Clinical Electrophoresis Can accommodate cellulose acetate strips up to 24 x 20cm Complete range of cellulose acetate gels and kits Range includes Densitometer, Scanner and Software Typical application: Clinical Electrophoresis Tests: haemoglobin, serum proteins for monoclonal gammopathies, urine proteins, isoenzymes, lipo and glycoproteins and for immunofixation. Cellulose acetate electrophoresis is an important technique in clinical diagnostics. This range offers a complete system solution for research and clinical procedures. The Clarit-E Cellulose Acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis. The unit includes an adjustable support which enables easy and fast adjustment for different lengths of cellulose acetate strip. Strip dimensions 24 x 20cm (W x L) Unit dimensions 30 x 30cm Cellulose Acetate System EL6000 Clarit-E cellulose acetate electrophoresis unit EL6047 Glob Al Scan software densitometer (computer and scanner not included) EL6048 Flatbed scanner for Glob Al Scan Accessories EL x 14 CellasGEL 250 micron EL x 14 CellasGEL 200 micron EL x 17 CellasGEL 200 micron EL x 14 CellasGEL 250 micron EL x 14 CellasGEL 200 micron EL x 17 CellasGEL 200 micron EL6034 Applicator Semimicro 2+2 for CellasGEL 2.5 x 14cm EL6038 Applicator Micro 8 for CellasGEL EL6051 Bridge 11cm for CellasGEL sheets 17 x 17cm EL6054 Bridge 11cm for CellasGEL strips 5.7 x 17cm Other products are available in this range, please enquire 11

12 Vertical Electrophoresis Systems clearly better resolution. Rapid Gel Casting and Loading ensures trouble free gel set-up Versatile modules for slab gel, 2-D electrophoresis & electroblotting using a single universal buffer tank Simple, rapid, leak proof gel casting (unique sliding gates gel clamping system is only applicable for Mini vertical tank) Insert glass plates and slide gates to create efficient seal Transfer to casting base and tighten cams Insert gel solution and comb and allow to polymerise Transfer to tank, fill with buffer and run Pressure clamping frame On the Mini gel tank a unique single piece pressure frame offers the option of rapid single screw per side or new screw free set up. Even pressure and buffer contact across the plates results in even sample migration and efficient heat removal from the gel. Glass plates are 2mm thick for Mini and 4mm thick for Mini Wide and Maxi tanks to reduce breakage. Run up to four gels at a time While most vertical gel units can run only one or two gels, the Clarit-E Mini units can run one, two or up to four gels at any time using a triple glass plate sandwich. Effective buffer cooling A simple to use cooling pack system ensures enhanced resolution without costly and time-consuming additional equipment. No chiller, tap or obstructing connecting leads are required. The cooling pack is simply pre-chilled in a freezer and placed in the gel tank. Additionally, the use of cooling packs reduces buffer volume. Extensive range of combs Up to five thicknesses, colour coded: Purple: 0.5mm for ultra resolved bands Black: 0.75mm for ultra resolved bands White: 1mm supplied as standard Red: 1.5mm for maximising sample volume Blue: 2mm for maximising sample volume Options for sample prep Options for multichannel pipette loading The number of samples can be maximised using combs with higher numbers of teeth. 12

13 The Clarit-E range of Vertical Gel Units combines ease of use with high resolution separations. Three sizes, Mini 10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm, share a host of common features including a guaranteed leak-proof seal required for trouble free, rapid and uncomplicated gel casting. Utilising a built in gel running module eliminates time-consuming transfer of glass plates following casting, a process which can cause gel damage and misalignment. Glass plates with permanently bonded spacers, guarantee perfect spacer alignment. The glass plate sandwich is simply inserted between pressure bars and a minimum of screws tightened. For the Clarit-E Mini there are no screws to tighten as the unique gel clamping system ensures fast set up times and guaranteed leak-proof gel casting. Following polymerisation, the gel running module is simply inserted into the gel tank for electrophoresis. Interchangeable Modules Running Module 2D Module Blotting Module with double-hinged cassettes for added convenience Corrosion resistant, 99.99% pure platinum electrodes and gold plated terminals easily replaced, ensuring minimum repair times. Retractable connectors compatible with most major power supplies. All units are CE marked and are in conformity with the relevant CE Safety Directives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC :2001. EN :2001; 89/336 /EEC EMC DIRECTIVE: EN55011 Emissions, EN Immunity. 13

14 Mini Injection Moulded construction Compatible with all 8 x 10 and 10 x 10cm precast gels Low buffer volumes Run up to 4 gels Typical application: Mini SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations. The preferred unit for routine mini protein electrophoresis, the Clarit-E Mini is constructed using the latest injection moulding manufacturing techniques. This gives a high quality, low cost product with unsurpassed finish, durability and strength. Clarit-E Mini and optional accessories The running insert has the option of sliding gates or a single screw per side for clamping gels. Accessory electroblotting and tube gel modules are available which use the same outer tank and lid. See Modular systems for further information. Internal running module Plate dimensions, 10 x 10cm Gel dimensions, (W x L) 7.5 x 8cm Unit dimensions (W x D x H) 19 x 13 x 15cm Max. sample capacity 80 samples, per unit 20 samples per gel Buffer volume Min 250ml; Max 1200ml Combs available : No. of samples 1, 5, 8MC, 9, 10, 12, 16MC, 20 Thicknesses 0.75, 1, 1.5, 2mm Mini Gel loading guides EL2004 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers, 2 x 12 sample combs, cooling pack, dummy plate. EL2000 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers, 2 x 12 sample combs, cooling pack, dummy plate and casting base. EL2012 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers, 2 x 12 sample combs, cooling pack, dummy plate, casting base and external casting upstand. Integral levelling feet and level bubble Multiple Mini Gel Casting Standard and gradient gels can be poured Choice of 2, 6, 12 or 24 gel casting models Advance casting of multiple mini gels can help to achieve consistent results between runs. These multiple gel casting systems are tailored for use with Clarit-E Mini vertical electrophoresis units; with three models of 2, 6, 12 and 24 gel capacities. Fewer gels can be poured if required using the acrylic saver blocks supplied with each system. The fixed hinged clamps allows the gel sandwich to be adjusted to the correct pressure irrespective of the number or the thickness of gels being poured. Separation sheets allow the easy separation of the glass plates after pouring. Multiple Mini Gel Casting Catalogue EL2018 EL2164 EL2166 EL gel caster for 8 x 10cm or 10 x 10cm gels 6 gel caster for 8 x 10cm or 10 x 10cm gels 12 gel caster for 8 x 10cm or 10 x 10cm gels 24 gel caster for 8 x 10cm or 10 x 10cm gels 14

15 Mini Wide Maxi Offers the capacity of two mini gels on a single gel Optional low or high buffer volumes Typical application: Mini SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations. The Clarit-E Mini Wide vertical gel unit with a gel width of 20cm effectively allows double the number of samples to be resolved as the Clarit-E Mini unit. This allows consistency of sample comparison on a single gel and is designed for those with greater than 20 samples to compare and resolve. Both the Mini Wide and the Maxi system use 4mm thick glass plates which reduce breakage and have bonded spacers for added convenience. A wide range of accessories is available allowing many techniques to be performed using the same unit. Prep combs can be used to maximise sample loading and recovery. Uses same combs as Maxi systems Ideal for high resolution separations Optional low or high buffer volumes Typical application: Maxi SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations. The preferred unit for maxi protein electrophoresis, the Clarit-E Maxi unit combines convenient ease of use features with high resolution separations. Plate dimensions 20 x 10cm Gel dimensions (W x L) 18 x 8cm Unit dimensions (W x D x H) 26 x 16 x 16cm Max. sample capacity 192 samples per unit 48 samples per gel Buffer volume Min 600ml; Max 2800ml Combs available : No. of samples 1, 5, 10, 18MC, 24, 30, 36MC, 48 Thicknesses 0.75, 1, 1.5, 2mm Plate dimensions 20 x 20cm Gel dimensions (W x L) 16 x 17.5cm Unit dimensions (W x D x H) 26 x 16 x 28cm Max. sample capacity 192 samples per unit 48 samples per gel Buffer volume Min 1200ml; Max 5600ml Combs available : No. of samples 1, 5, 10, 18MC, 24, 30, 36MC, 48 Thicknesses 0.75, 1, 1.5, 2mm Mini Wide Maxi Catalogue EL2083 EL2084 EL2088 Clarit-E Mini Wide, 20 x 10cm 2 sets of glass plates with bonded 1mm thick spacers, 2 x 24 sample combs, cooling pack Clarit-E Mini Wide, 20 x 10cm 2 sets of glass plates with bonded 1mm thick spacers, 2 x 24 sample combs, cooling pack including casting base Clarit-E dual caster, 20 x 10cm Catalogue EL2120 EL2100 EL2110 Clarit-E Maxi, 20 x 20cm dual with glass plates with bonded 1mm thick spacers, 2 x 24 sample combs, cooling pack, dummy plate Clarit-E Maxi, 20 x 20cm dual with glass plates with bonded 1mm thick spacers, 2 x 24 sample combs, cooling pack, dummy plate and casting base Clarit-E dual caster, 20 x 20cm 15

16 Maxi Plus for Second Stage 2-D The 26cm active gel width provides a large gel area to resolve large IEF strips. In combination with the IEF system, this offers a complete package for 2-D electrophoresis. The unit utilises the Clarit-E advanced design features to provide convenient ease of use with high resolution separations. Rapid set up cooling retains resolution in extended separations and also saves on buffer volume without affecting run quality. Four gels can be resolved per run. A wide range of accessories is available to allow easy transition between 2-D and standard vertical electrophoresis techniques. In particular different types of 2-D comb allow a wide degree of versatility in sample selection and gel set-up. Unit dimensions (W x H x D) 36 x 33 x 18cm Plate dimensions (W x L) 30 x 22cm Gel dimensions (W x L) 28 x 20cm Max sample 300 samples per run; 75 samples per gel Buffer volume Min 1800ml, Max 8400ml Combs available: No. of teeth 1, 2, 4, 28MC, 56MC, 75 Thicknesses 0.25, 0.35, 0.5, 1, 1.5, 2.0mm Maxi Plus for second stage 2-D EL2390 Clarit-E Maxi Plus, 30 x 22cm dual with glass plates with bonded 1.5mm spacers, 2 x 28 sample combs, 2 x 2-D sample combs, cooling pack, dummy plate EL2395 Clarit-E Maxi Plus, 30 x 22cm dual with glass plates with bonded 1.5mm spacers, 2 x 28 sample combs, 2 x 2-D sample combs, cooling pack, dummy plate and casting base EL2334 Clarit-E Maxi Plus blot insert - includes 3 cassettes and 6 fibre pads EL2318 Clarit-E dual caster Maxi Plus DNA Sequencing Unit Run up to 96 samples Enhanced gel heat homogenisation Variable low or high buffer volumes 20 x 50cm or 33 x 45cm formats Typical application: DNA Sequencing, 2-D analysis, Micro-satellite analysis, DNA fingerprinting, Gel shift assays, Single- Strand Conformation Polymorphism (SSCP), Heteroduplex and Oligonucleotide analysis. Ideal for a variety of large format vertical gel applications, these units offer advanced features for enhancing gel resolution and ease of use, essential when handling gels of this size. Each unit contains ultra soft silicone seals for easy plate sealing and trouble-free runs, even over extended run times. Resolution is enhanced by using an aluminium heat sink plate, which is essential for even sample migration. Added convenience is provided by a removable lower buffer tank and upper buffer drainage tap. Special buffer chambers allow either low buffer volumes to be used for economy or high buffer volumes to be used for extended runs. A wide range of interchangeable comb and spacer options allows a large number of techniques to be easily accomplished. Plate dimensions (W x L) EL x 50cm EL x 45cm Max sample capacity EL samples EL samples Buffer volume EL2560 Min 500ml, Max 1000ml EL2580 Min 800ml, Max 2000ml Combs available No. of teeth 24, 48, 80, 96 Thicknesses 0.25, 0.35, 1, 1.5mm DNA Sequencing Unit EL2560 Clarit-E DNA sequencing unit, 20cm wide, glass plates, 0.35mm spacers, 48 sample comb EL2580 Clarit-E DNA sequencing unit, 33cm wide, glass plates, 0.35mm spacers, 48 sample comb EL2594 Fan heater sensor kit for DNA sequencing unit EL2560 and EL

17 Combs for Clarit-E Vertical Gel Systems Extensive range of combs and spacers Up to five thicknesses, colour coded: Purple: 0.5mm for ultra resolved bands Black: 0.75mm for ultra resolved bands White: 1mm supplied as standard Red: 1.5mm for maximising sample volume Blue: 2mm for maximising sample volume Options for Sample Prep Options for multi channel pipette compatibility The number of samples can be maximised using high tooth number combs. Loading guides are available for some comb options, please enquire Clarit-E Vertical Mini EL2000, EL2002, EL2004, EL2006, EL2008, EL2010, EL2012 from page mm thickness 0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume Volume 5mm 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel thick gel 1 Prep, 1 Marker EL µl EL µl EL µl EL µl EL µl 5 EL µl EL µl EL µl EL µl EL µl 8 MC EL2000-8MC µl EL2000-8MC µl EL2000-8MC-1 60µl EL2000-8MC µl EL2000-8MC-2 120µl 9 EL µl EL µl EL µl EL µl EL µl 10 EL µl EL µl EL µl EL µl EL µl 12 EL µl EL µl EL µl EL µl EL µl 16 MC EL MC µl EL MC µl EL MC-1 25µl EL MC µl EL MC-2 50µl 20 EL µl EL µl EL µl EL µl EL µl Clarit-E Vertical Mini Wide/Maxi EL2083, EL2084, EL2100, EL2120 from page mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel 1 Prep, 1 Marker EL µl EL µl EL µl EL µl 5 EL µl EL µl EL µl EL µl 10 EL µl EL µl EL µl EL µl 18 MC EL MC µl EL MC-1 50µl EL MC µl EL MC-2 100µl 24 EL µl EL µl EL µl EL µl 30 EL µl EL µl EL µl EL µl 36 MC EL MC µl EL MC-1 25µl EL MC µl EL MC-2 50µl 48 EL µl EL µl EL µl EL µl Clarit-E Vertical Maxi Plus EL2390, EL2395 from page mm thickness 1.5mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Volume Volume 5mm 5mm thick gel thick gel 1 Prep, 1 Marker EL µl EL µl 2 EL µl EL µl 4 EL µl EL µl 28 MC EL MC-1 80µl EL MC µl 56 MC EL MC-1 40µl EL MC µl 75 MC EL MC-1 25µl EL MC µl Combs also available in other thicknesses and number, please enquire 17

18 Combs for Clarit-E 20cm wide large vertical format unit EL2560 from page mm thickness 0.35mm thickness 1mm thickness 1.5mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel 24, Sharks tooth EL µl EL µl EL µl EL µl 48, Sharks tooth EL µl EL µl EL µl EL µl Combs for Clarit-E 33cm wide large vertical format unit EL2580 from page mm thickness 0.35mm thickness 1mm thickness 1.5mm thickness No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Volume Volume Volume Volume 5mm 5mm 5mm 5mm thick gel thick gel thick gel thick gel 24, Sharks tooth EL µl EL µl EL µl EL µl 80, Sharks tooth EL µl EL µl 96, Sharks tooth EL µl EL µl The list above includes the most popular combs for these units. Many more options are available, please enquire. Accessories for Vertical Systems A range of Glass Plates and Spacers at a glance Item Mini Mini Wide Maxi Maxi Plus 20cm seq. 33cm seq. Cooling Pack EL2040 EL2140 EL2342 Notched Glass Plates EL2042 EL2112 EL2142 EL2364 EL2562 EL2582 Plain Glass Plates EL2044 EL2116 EL2144 EL2366 EL2564 EL2584 Plain Plates +0.5mm Spacers EL2046 Plain Plates +0.75mm Spacers EL2048 EL2118 EL2148 EL2374 Plain Plates +1mm Spacers EL2050 EL2121 EL2150 EL2378 Plain Plates +1.5mm Spacers EL2052 EL2122 EL2152 EL2382 Plain Plates +2mm Spacers EL2054 EL2125 EL2154 EL2384 Notched Plates +0.5mm Spacers EL2056 Notched Plates +0.75mm Spacers EL2058 EL2127 EL2158 EL2368 Notched Plates +1mm Spacers EL2060 EL2129 EL2159 EL2376 Notched Plates +1.5mm Spacers EL2062 Notched Plates +2mm Spacers EL2064 Dummy Plate EL2066 EL2156 EL2157 EL2386 Spacers -0.25mm EL2566 EL2586 Spacers -0.35mm EL2568 EL2588 Spacers -0.5mm EL2068 EL2068 Spacers -0.75mm EL2070 EL2070 EL2170 EL2388 Spacers -1mm EL2072 EL2072 EL2172 EL2392 EL2570 EL2590 Spacers -1.5mm EL2074 EL2074 EL2174 EL2394 EL2572 EL2592 Spacers -2mm EL2076 EL2076 EL2176 EL

19 Isoelectric Focusing Modular Systems For IEF strip and gel applications Large cooling platform area Pick and place easy to position electrodes Grid for accurate electrode alignment The IEF system has been designed to allow the full range of IEF flat bed electrophoresis techniques. These include wick based electrophoresis, precast horizontal IEF gel electrophoresis, hand cast horizontal IEF Gel electrophoresis and Iso Electric focusing using IEF strips. The unit includes enhanced cooling over a large platform which offers increased strip capacity and gel area. Uniform cooling of the platform is achieved using a large ceramic plate which can be connected to any standard chiller via quick fit connectors for enhanced gel or strip cooling. Economy of bench space and cost Enhanced reproducibility Simple to use casting Rapid set up cooling The Clarit-E range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main tank and lid, three different inserts are interchangeable for slab gel, tube gel and electroblotting techniques. These systems are available in three size options, Mini 10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm and are available as individual, combination or complete modular systems. Combination and modular systems offer maximum value and space and have the added benefit of increased consistency, which results from using the same universal base and lid. Max strip length Max gel dimensions Unit dimensions (W x D x H) 26cm 26 x 26cm 55 x 35 x 10cm Isoelectric Focusing Catalogue EL7000 EL7010 Clarit-E Flatbed IsoElectric Focusing System 26 x 26cm Chiller 19

20 Mini, Mini Wide and Maxi complete Modular Systems These systems include all modules and accessories required for Slab Gel Electrophoresis, 2-D Electrophoresis and Electroblotting. The central component is the Clarit-E Mini, Mini Wide or Maxi Vertical unit. These include a rapid and intuitive casting system, enhanced and easy to set up cooling system and have increased capacity the Mini can run up to four gels per run. In addition, the Clarit-E Tube gel module is capable of resolving up to 10 first dimension gels and the Electroblotting module has a 3 blot capacity. Tube Gel Units, Inserts and Accessories These stand-alone, complete units for first dimension capillary/tube gel electrophoresis are available in 3 sizes, Mini 10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm formats. Units are interchangeable with Clarit-E slab gel and electroblotting inserts. Complete Modular Systems include: Vertical tank with gel running module, 2x notched glass plates, 2x plain glass plates with bonded spacers, dummy plate, 2x combs, casting base, silicone mat, cooling pack, running module for tube gels, capillary tubes, blanking plugs, 2-D combs, spacers, electroblotting module, 3x compression cassettes and 6x fibre pads. 2-D Complete Systems Rapid set-up electrofocusing 10 tube capacity Extended accessory range Enhanced cooling features The Clarit-E 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete Mini, Mini Wide or Maxi 2-D system. The Tube Gel Module includes a rapid release gasket for easy tube extraction. Focusing can be accomplished in as little as three hours in the Mini Unit. Disposable capillary tubes are included for added convenience, plus 2-D combs and spacers, which are colour coded according to thickness for easy identification. Complete 2-D System comprising: Vertical tank with gel running module, 2x notched glass plates, 2x plain glass plates with bonded spacers, dummy plate, 2x combs, casting base, silicone mat, cooling pack, running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers. Clarit-E Tube Gel Units comprise tank and lid, glass capillary tubes, blanking ports and cooling pack. Modular Electroblotting Hinged cassettes 3 blot capacity High intensity electrodes Rapid set-up cooling The Clarit-E Electroblotting Systems include both modules required for Slab Gel Electrophoresis and Electroblotting and accessories, to provide a complete Mini, Mini Wide or Maxi gel casting, running and electroblotting system. Modules included in the Clarit-E Maxi 2-D system Modules included in the Clarit-E Mini Wide 2-D system A high intensity current is generated by coiled platinum electrodes. These in conjunction with the advanced cooling features allow rapid electroblotting in as little as one hour. Clarit-E Modular electroblotting system comprises: Vertical tank with gel running module, 2x notched glass plates, 2x plain glass plates with bonded spacers, dummy plate, 2x combs, casting base, silicone mat, cooling pack, electroblotting module, 3x compression cassettes and 6x fibre pads. 20

21 Electroblotting Units Ideal for Western blotting of proteins Open design, rigid cassettes for maximum current transfer These stand-alone, complete units for electroblotting, Mini, Mini Wide and Maxi formats, include Clarit-E tank and lid, electroblotting insert, cassettes and fibre pads. Units are interchangeable with Clarit-E slab and tube gel inserts. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained. Accessories for Clarit-E Vertical Systems Gradient Mixers Ideal for Caesium, Sucrose and Gel gradients these Gradient Mixers comprise two chambers a reservoir and a mixing chamber with an interconnecting valve. A second valve regulates the output flow from the mixing chamber. All mixers have a flat base which allows them to be placed on a magnetic stirrer. A magnetic stirring bar can be placed directly in the mixing chamber to ensure a constant gradient. The support rod allows the mixer to be fixed to a retort stand for extra stability. Unit dimensions: Mini 19 x 13 x 15cm (W x D x H) Mini Wide 26 x 16 x 16cm Maxi 26 x 16 x 28cm Max. sample capacity: Mini Slab 80 samples, 20 samples /gel, Tube - 10 tubes; Blot - 3 blots Mini Wide Slab 192 samples, 48 samples /gel, Tube - 10 tubes; Blot - 3 blots Maxi Slab 192 samples, 48 samples /gel, Tube - 10 tubes; Blot - 3 blots Buffer volume: Mini Min 250ml; Max 1200ml Mini Wide Min 600ml; Max 2800ml Maxi Min 1200ml; Max 5600ml Electroblotting Units Catalogue Mini Mini Wide Maxi Complete Electrophoresis System Includes tube gel, EL2200 EL2250 EL2300 blotting and vertical system EL2320 EL2330 EL2340 Complete 2-D Electrophoresis System Includes tube and vertical system EL2026 EL2096 EL2126 Tube Gel Unit, with capillary tubes, blanking ports and cooling pack EL2028 EL2098 EL2128 Tube Gel Insert includes glass tubes and blanking ports EL2030 EL2030 EL2130 Capillary Tubes EL2356 EL2356 Capillary Tubes 1.5mm EL2032 EL2032 EL2032 Capillary Blanking Ports EL2400 EL2450 EL2500 Complete Blotting System includes vertical system and electroblotting module EL2402 EL2452 EL2502 Blotting System includes tank, 3 cassettes, 6 fibre pads and cooling pack EL2034 EL2114 EL2134 Blotting insert includes 3 cassettes and 6 fibre pads EL2036 EL2106 EL2136 Blotting cassette EL2038 EL2458 EL2138 Fibre pads Gradient Mixers EL ml Gradient Mixer EL ml Gradient Mixer EL ml Gradient Mixer EL ml Gradient Mixer EL ml Gradient Mixer Plate Racks These sturdy racks are designed for safe drying and storage of glass plates. The small rack can hold up to 20x 2mm thick plates while the larger rack can accommodate up to 10x 5mm thick glass plates. Plate Racks EL2630 Mini glass plate rack for 20 x 2mm plates EL2640 Large glass plate rack for 10 x 5mm plates 21

22 Tank Sub Electroblotters Semi Dry Electroblotters Ideal for wet electroblotting of proteins Western blotting Up to five gel blot cassettes utilised at any one time Hinged cassettes for added convenience Accommodates gel thicknesses from 0.25 up to 3mm Tank Sub Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems. This is especially useful in high throughput laboratories. A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette s open architecture ensures the maximum blot area allows direct transfer of current. Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent ph drifts in the buffer due to incomplete buffer mixing. Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy. Rapid transfer times Western, Southern and Northern Blots Economic Transfers due to very low buffer volumes Screw down lid Gels from 0.25 up to 10mm thick can be blotted These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting - typically 15 to 30 minutes. All units can be used for all types of blotting via uncomplicated buffer and set up procedures. Each unit is compatible with their respective Clarit-E vertical gel system. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arching or damage. Unit dimensions: Mini 19 x 13 x 19cm (W x D x H) Maxi 24 x 16 x 26cm Max. sample capacity: Mini 5 Blots, 10 x 10cm Maxi 5 Blots, 20 x 20cm 20 Blots, 10 x 10cm Buffer volume: Mini Min 1000ml; Max 1500ml Maxi Min 4300ml; Max 6000ml Tank Sub Electroblotters Unit dimensions: Mini 16 x 16 x 7cm (W x L x H) Maxi 26 x 26 x 7cm Maxi Plus 39 x 51 x 7cm Maxi Long 26 x 56 x 7cm Max. sample capacity: Mini 1 Blot, 10 x 10cm Maxi 1 Blot, 20 x 20cm 4 Blots, 10 x 10cm Maxi Plus 1 Blot, 35 x 45cm Maxi Long 1 Blot, 20 x 50cm Buffer volume: Mini 5ml Maxi 20ml Maxi Plus 75ml Maxi Long 50ml Catalogue EL2412 EL2512 EL2036 EL2038 EL2136 EL2138 Electro Blot Mini, 10 x 10cm System for five cassettes, with tank and lid, 5x cassettes, 12x fibre pads and cooling pack Electro Blot Maxi, 20 x 20cm System for five cassettes, with tank and lid, 5x cassettes, 12x fibre pads and cooling pack Tank Blot Mini Cassette Fibre pads - pk/6 Tank Blot Maxi Cassette Fibre pads - pk/6 Semi Dry Electroblotters EL2460 Semi Dry Mini Blotter 10 x 10cm system EL2465 Semi Dry Maxi Blotter 20 x 20cm system EL2470 Semi Dry Maxi Plus Blotter 20 x 50cm system EL2475 Semi Dry Maxi Long Blotter 33 x 45cm system 22

23 Capillary Blotting Dot and Slot Blotters Efficient transfer of high and low molecular weight fragments Fast, easy set-up DNA transfer in 2 hours or less; RNA transfer in 3 hours or less Floating lid ensures even pressure without gel compression These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized permanent wicks, which eliminate the requirement for overlapping wicks, offering greater consistency. The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for faster transfer times. Side by side transfer of multiple gels can be performed in a single set up. Low cost Simple construction Easy assembly Alphanumeric sample identification Four different sample number size and styles of Hybridisation Manifold are offered: 48 or 96 well dot blotter and 24 or 48 well slot blotters. Typical applications include clone screening with DNA/RNA probes in Southern/Northern blots and immunological screening with antibodies in western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lateral transfer of samples smudging by ensuring that a complete vacuum is formed. Six thumbscrews ensure even and tight sealing for fast sample suck down. Available in 15 x 26cm and 28 x 26cm formats. Principle: Through capillary action, the buffer travels from the reservoir to the absorbent towelling via the porous supports, permanent wick, gel and membrane. This action optimises the transfer of low and high Molecular Weight DNA/RNA from gel to membrane without mechanical damage to even the thinnest of gels. Unit dimensions: Mini 30 x 30 x 10cm (W x D x H) Maxi 42 x 30 x 10cm Capillary Blotting EL2485 Capillary Blotting Unit (15cm x 26cm) (Mini) EL2480 Capillary Blotting Unit (28cm x 26cm) (Maxi) Model EL2448 EL2496 EL2524 EL2548 Configuration 3 x 16 8 x 12 2 x 12 3 x 16 Size of well 6mm diam. 6mm diam. 6 x 0.5mm 6 x 0.5m 12mm deep 12mm deep 12mm deep 12mm deep Vacuum required 600mg Hg 0.8 BAR with cold trap Unit dimensions 60 x x x x 95 x 100cm x 140cm x 83cm x 100cm Membranes 12.1 x 4.4cm 11 x 7.4cm 12.1 x 4.4cm 12.1 x 4.4cm size required Dot and Slot Plotters EL well Dot Blot Manifold, 3 x 16 array EL well Dot Blot Manifold, 8 x 12 array EL well Slot Blot Manifold, 2 x 12 array EL well Slot Blot Manifold, 3 x 16 array 23

24 Maxi Power Supplies Midi Power Supplies High performance, easy to operate Graphic and text displays Programme constant voltage and current Memory mode Stores 16 files to recall and re-run Safety Comply to all relevant safety standards The Clarit-E Maxi Power Supplies can handle virtually any application from high voltage electrophoresis to high current blotting. Two models provide versatility, offering maximum output capability from 300V to 500V, 600mA to 3A and 200 to 300 watts. These units are suitable for running multiple SDS gels, blotting and large submarine gels. Fully Programmable: parameter entry is via the membrane keypad and the start key. Programming includes recall and run constant or multi-step electrophoresis in a variety of operational modes: 1. Constant operation with timer - programme constant voltage, current or wattage with timer to eliminate the possibility of over running samples in gels. 2. Memory mode Store 16 files to recall and re-run favourite multi step programmes. Display: The large, 128 x 64 dot matrix display screen allows both graphic and text visualisation. Set parameters will be displayed continuously compared with actual run conditions at the touch of a button. Programme constant voltage and current Alarm can be set at the end of the programme Auto cut-off function Current up to 700mA These inexpensive, compact Clarit-E Midi power supplies are ideal for routine horizontal and mini vertical electrophoresis. Microprocessor control allows user programming, of up to ten programs, and can be set-up to run in constant voltage and constant current modes. Four gel chambers can be connected at any one time. An optional timer can be set to end the run and sound an alarm. Safety features include shrouded plugs and sockets, no load detection and ground leakage detection. Safety: The alarm will sound in the event of ground leakage, no load or overload. Maxi EL2740 EL2750 Max. Voltage 500V 300V Max. Current 800mA 3A Max. Watt 300W 300W Output Type Constant Voltages ; Constant Current ; or Constant Watt Program Storage 16 programmed files Program Multi-Step Up to 6 steps Timer mins Display 28 x 64 dot matrix display screen Safety No load ; Leakage and Overload Detection Dimensions 18 (W) x 28 (L) x 14cm (H) Operation Voltages 110/220V Midi EL2720 EL2730 Max. Voltage 250V 300V Max. Current 700mA 700mA Max. Watt 150W 150W Output Type Constant Voltage or Current Program Storage 10 methods 10 methods Timer mins mins Dimensions 16 (W) x 14 (L) x 7cm (H) Operation Voltages 110/220V Maxi Power Supplies Midi Power Supplies Catalogue EL2740 EL2750 Clarit-E Maxi Power Supply, 500V, 800mA, 300W Clarit-E Maxi Power Supply, 300V, 3000mA, 300W Catalogue EL2720 EL2730 Clarit-E Midi Power Supply, 250V, 700mA, 150W Clarit-E Midi Power Supply, 300V, 700mA, 150W 24

25 Mini Power Supplies Compact size Safety design; shrouded plug and socket; no load detection Constant voltage Low cost and economical Clarit-E Mini power supplies have been developed specifically for horizontal gel electrophoresis systems. These ultra compact power units with dual outlets, are recognised as amongst the most compact and inexpensive power supplies currently available and are designed and constructed to the most rigorous safety standards. Mini EL2700 EL2710 Max. Voltage 150V 300V Max. Current 600mA 400mA Max. Watt 60W 60W Output Type 25; 50; 75; 100; 125; 150V 50; 100; 150; 200; 250; 300V Safety No load detection Dimensions 10.5 (W) x 15 (L) x 7.5cm (H) Operation Voltages 110/220V Mini Power Supplies EL2700 Clarit-E Power Supply, 150V, 600mA, 60W EL2710 Clarit-E Power Supply, 300V, 400mA, 60W Accessories for Power Supplies EL2701 Power supply adapters, 4mm to 4mm EL2702 Power supply adapters, 4mm to 2mm EL2703 Power supply adapters, 2mm to 4mm Gel Drying Dryer With a drying area of 21 x 31 cm, the Midi gel dryer can dry six 10 x 10cm gels or a single larger gel. The Maxi Gel Dryer with a 35 x 45cm drying area can dry twelve 10 x 10cm mini gels simultaneously. The unit s microprocessor controls temperature and time, each parameter being displayed on its own LED display. The gels are heated from the base plate while the vacuum removes the moisture from below to dry the gel homogeneously. These dryers feature optimal sealing using a silicone rubber cover and supporting mask. When applying the vacuum, a groove that frames the drying surface provides an optimal tight seal during the drying. Dryers Temperature Increment 0.1 C Temperature Calibration Yes Temp Uniformity ± 0.2 C Timer mins Drying area Midi 21 x 31cm, Maxi 35 x 45cm Operating Temp. Range Ambient to 90 C Dimension W x L x H Midi 30 x 36 x 8cm, Maxi 44 x 50 x 8cm Pump Condenser vapour capacity 1.5L Dimensions W x L x H 35.5 x 28.5 x 39cm Vacuum Pump Two pumps in one vapour trap and high efficiency diaphragm pump Environmentally friendly traps and contains vapours The GelMaster Vacuum Pump features two pumps in one package: a room temperature vapour trap acts as a pump by condensing the hot vapours from the gel dryer and trapping them from the rest of the systems; this is followed by a high efficiency diaphragm pump, delivering a powerful, non-fluctuating vacuum for fast, smooth drying, which acts as the primary vacuum source. The pump is so efficient, one GelMaster can be connected to three gel dryers simultaneously. Its 1.5L capacity allows between gels to be dried before the trap needs to be drained. The GelMaster trapping medium can be used repeatedly. Gel Drying EL8800 Midi Gel Dryer 220V, 21 x 31cm EL8810 Maxi Gel Dryer 220V, 35 x 45cm EL8815 Gelmaster Vacuum Pump EL8816 Replacement Trapping fluid for Gelmaster EL8817 Replacement flushing fluid for Gelmaster 25

26 Gel Documentation System 14.7 mega pixel digital camera Image is viewed from a 8" TFT colour monitor Lightweight compact hood with easy access door and inner built-in lights Safety door switch Includes flash card, flash card reader and Ethidium bromide filter The Clarit-E Gel Documentation system is simple, inexpensive and ultra compact. It contains a 16 bit CCD digital camera with a superb resolution of 14.7 mega pixels. For added convenience, limited space and budget requirements, it can be used computerfree. A variety of images can be captured from agarose and other fluorescent gels, colorimetric gels, autoradiography film and blotting membranes. The system is fitted with a 55mm ethidium bromide filter and has a safety switch to turn off the UV transilluminator when the door is opened. Files can be saved in a variety of formats for transfer to computer systems for storage and analysis. The Clarit-E Gel Documentation system can be directly connected to a thermal printer - EL or to many other commercially available printers. Camera: Type 5x optical / 4x digital zoom camera Effective Pixels 14.7 megapixels CCD 1/1.7" high-density CCD Maximum Aperture f/2.8 (W) ~ f/4.5 (T) Shutter Speed 15 ~ 1/4000s Filters +3 Close up and EtBr; optional SYBR green Storage Media 2MB memory card Computer Interface USB 2.0 Hi Speed (mini-b jack) Video Out NTSC/PAL Darkroom: Multi-power Source For camera, inner white LED, TFT screen Inner White Light 2x3 W LED Safety Device Safety door switch Side Access None Weight & Dims 6.1kg; 290 x 220 x 320mm Voltage Rating 110~220V Screen: Type 8"TFT Resolution 600 x 800 Pixels Brightness 350 cd/mm2 Constant Ratio 300 : 1 Display Mode NTSC / PAL / SECAM mode auto switching Gel Documentation System EL4000 Clarit-E gel doc system, including 12 mega pixel digital camera, camera adaptor, 5.7 TFT colour screen, ethidium bromide filter, camera hood. EL4020 Clarit-E gel doc system with UV Transilluminator, 254nm EL4010 Clarit-E gel doc system with UV Transilluminator, 312nm EL4030 Clarit-E gel doc system with UV Transilluminator, 365nm EL4040 Clarit-E gel doc system with UV Transilluminator, 254/312nm EL4050 Clarit-E gel doc system with UV Transilluminator, 254/365nm EL4080 Ethidium Bromide filter EL4090 SyBr Green Filter EL4070 Thermal Printer EL4075 Replacement paper for thermal printer. EL4100 UV to white light Transilluminator screen converter EL4110 White light box for Clarit-E Gel Doc Gel Cutting Tips A great new tool for Gene Cloning Rapid, precise and contamination free excision of spots and bands from gels Eliminates scalpel damage to transilluminator or gel tray Designed for removing bands from agarose and protein gels Easy gel slice transfer tips fit standard 1000µl pipettors Autoclavable DNase and RNase free Sterile racked versions are also available Gel cutting tips, newly available from Alpha Labs. No more scalpels required, these tips offer a unique and convenient way to excise bands from gels. Gel cutting tips allow complete single handed and contamination free removal of the gel band of interest. The gel band is neatly extracted and contained in the tip. It can then be simply dispensed into a tube and the used tip ejected and disposed of. Gel Cutting Tips EL2650 Gel cutting tips 4mm x 1mm 250 / bag EL2650R Gel cutting tips 4mm x 1mm 5x rack of 48 EL2660 Gel cutting tips 6.5mm x 1mm 250 / bag EL2660R Gel cutting tips 6.5mm x 1mm 5x rack of 48 26

27 Transilluminators Electrophoresis Reagents Three wavelength options: 254/312/365 nm two Dual wavelength models Long life filter High efficiency reflector As new techniques emerge in molecular biology so does the spectrum of products available to meet the growing demands of researchers. Agarose gel electrophoresis, introduced in the late 1970 s now has a variety of different agarose types, each of which is optimally specified for particular applications. Hi/Lo intensity switch Fast start up These UV Transilluminators are multitasking workstations, which offer an ultra-violet source for the analysis of fluorescently stained DNA, RNA, and Protein electrophoresis gels. These also offer space to place tube racks, cutting tools or waste agarose gel, ideal when there is a need to cut bands. These new generation UV transilluminators include a specially designed safety UV leakage indicator which changes colour when UV light is detected. A new and high quality starter ensures that the UV tubes energise immediately and without flickering. This feature extends the life of the tubes. Agarose Universal Agarose Extra Agarose Reclaim Agarose Micro Plus Analytical Separation >1000bp Analytical Separation <1000bp PFGE ADN Typing Blotting Filter size 20 x 20 cm Light source: 8W x 6 tubes UV resistant plastic cover: 33 x 25cm (W x D) Unit Dimensions W x L x H 34 x 29.5 x 10cm Transilluminators EL4150 UV Transilluminator small, 21 x 21cm, 254nm EL4160 UV Transilluminator small, 21 x 21cm, 312nm EL4170 UV Transilluminator small, 21 x 21cm, 365nm EL4200 UV Transilluminator small, 21 x 21cm, 254/365nm EL4210 UV Transilluminator small, 21 x 21cm, 254/312nm Suitable Not Suitable Whatever your performance requirements we are confident that we can offer a suitable grade of agarose to suit your needs. There are 3 main categories of agarose available: Routine Agarose Good general separation characteristics at a reasonable price. (Agarose Universal). Low Melt Agarose Good separation characteristics coupled with low melt temperatures allowing easy recovery of bio-molecules (Agarose Reclaim). High Resolving Agarose Offering the best resolving characteristics, allowing comparison with toxic acrylamide when looking for detailed resolution. (Agarose Extra and Agarose Micro Plus). 27

28 Separation Ranges for Different Buffers with Application Guide Agarose 1x TAE buffer range (bp) Gel concentration (%) 1x TBE buffer range (bp) Universal 20,000 1, , , , , , , , , , , , Extra 40,000 3, ,000 2,000 22,000 2, ,000 1,500 15,000 1, ,000 1,000 10, , , , Reclaim 20, , , , , , , , , , , , Micro Plus 1, , , Nucleic acid analytical and preparative electrophoresis Blotting Protein electrophoresis such as radial immunodiffusion Conventional electrophoresis Pulsed field gel electrophoresis Blotting Agarose bead preparation Cell and enzyme immobilisation For applications that require efficient separation of small DNA fragments and PCR products Fragment separation in analytical and preparative electrophoresis Tissue and cell culture Viral plaque assays Agarose Specifications at a Glance Agarose Universal Agarose Extra Agarose Reclaim Agarose Micro Plus Moisture 7% < 7% 7% 7% Ash 4% < 0.25% 0.4% 0.35% EEO % < Sulphate < 0.1% < 0.12% 0.12% 0.11% Clarity (NTU) 4 <4 4 5 Gel Strength 1% (g/cm2) 1200 > (4%) Gel Strength 1.5% (g/cm2) 2500 > Gelling Temperature ( C) 36 ± Melting Temperature ( C) 88 ± DNase/RNase activity None detected None detected None detected None detected DNA resolution Finely resolved Finely resolved Finely resolved Finely resolved Gel background Very low Very low Very low Very low Agarose Pack Size EL0014 Agarose Universal 100g EL0015 Agarose Universal 500g EL0018 Agarose Extra 100g EL0020 Agarose Reclaim 100g EL0024 Agarose Micro Plus 25g EL0025 Agarose Micro Plus 100g 28

29 Acrylamide Precast Gels Compatibility Long 10 x 10cm and Short 10 x 8.2cm (WxH) formats guarantee compatibility with all major brands of mini gel electrophoresis systems currently on the market User Friendly the gel is used directly within the running module with no tape or comb to remove Rapid Loading each gel is lightly coloured allowing the user to load the entire sample each and every time into each well Traceable a unique identification code (UIC) allows each gel to be easily identified, even after staining Long Shelf-life 1 year at 4 C Clarit-E precast polyacrylamide gels are ideal for the separation of proteins and nucleic acids. They are exceedingly user friendly and may be handled directly, with no comb or tape to remove. Each well is clearly visible for easy loading and can accommodate a maximum sample volume of 35µl. Unlike other brands, no special tools are needed to access the gel following electrophoresis, as each gel cassette is opened quickly and easily by hand. Acrylamide Gel Specifications: Matrix: Acrylamide: Bis-acrylamide (30:0.9) Cassette Sizes (W x H): 10 x 10cm & 10 x 8.2cm Gel Separation Length: 83mm & 65mm Gel Thickness: 1mm Available Comb Configurations: 10 & 17 Wells Max. Voltage: 250V Storage: 4 C Shelf-Life: 12 months from date of manufacture Acrylamide Precast Gels Tris-Native Tris-SDS Denaturing Long 10x10cm Short 10x8.2cm Long 10x10cm Short 10x8.2cm MW Range (kda) % Acrylamide No. of Wells EL8101 EL8501 EL8310 EL % 10 EL8103 EL8503 EL8312 EL % 10 EL8105 EL8505 EL8301 EL % 10 EL8107 EL8507 EL8303 EL % 10 EL8109 EL8509 EL8306 EL % 10 EL8111 EL8511 EL8308 EL % 10 Also available with 17 Wells. Please enquire. Please Note: Precast Acrylamide Gels are only available to our UK based customers. 29

30 Buffers In electrophoresis a wide range of buffers and reagents are required in addition to the high quality gel media now available. These reagents must be of at least equal quality to the separation medium in use. Alpha Laboratories can offer a select range of convenience buffers. Without having to weigh, mix and adjust ph, convenience products avoid the simple errors, which can mean the loss of complete experiments due to the tedious nature of preparing routine solutions. The specifications offer a high degree of experimental security in laboratory work across a wide range of scientific disciplines. This means they satisfy the exacting demands of modern science and are ideal for use in electrophoresis. Purposefully developed and specified for these particular applications. They guarantee the kind of results expected with high quality chemicals. All these features mean these products are essential for the modern laboratory. Save time Reduce opportunities for error Allow high quality staff to be used more effectively Guaranteed consistent reagents All batches tested and validated prior to use Reduce exposure to hazardous materials The products are supplied as concentrates for simple dilution. TAE/TBE Buffers These buffers are widely used in the preparation of nucleic acid electrophoresis gels. All convenience buffers are formulated with 18 Megohm Water and 0.2 Micron Filtration. TAE Buffer (50x) is a concentrated solution of 2M Tris-Acetate and 100mM Sodium EDTA in distilled/deionised water (ph 8.3 at 1x concentration). TBE Buffer is available as a 10x concentrated buffer solution of Tris-Borate-EDTA in distilled/deionised water, containing 0.089M Tris base, 0.089M boric acid (ph8.3) and 2mM Na2EDTA. TAE/TBE Buffers Pack Size EL0077 TAE Buffer 50x conc. 1L EL0079 TBE Buffer 10x conc. 1L EL0080 TBE Buffer 10x conc. 4L TE Buffer TE Buffer is a 100x concentrated solution of 1 M Tris-HCl, ph8, with 100mM EDTA. Manufactured from ultra pure reagents, this buffer is the standard for DNA and RNA purification, processing and storage. TE Buffer Pack Size EL0081 TE Buffer 100x conc. 25ml SDS Solution Alpha Laboratories SDS solution (20%) is formulated using the highest quality SDS, that has been further purified to remove contaminants, which can interfere with spectro-photometric analysis and cause nonspecific binding. The purity of this product ensures that reproducible protein molecular weight measurements can be made with confidence, and optimizes activity recovery in enzyme purification procedures. The convenient pre-prepared solution eliminates the discomfort and hazards of working with powdered SDS. SDS Solution Pack Size EL0094 SDS Solution 20% (w/v) 100ml Tracking Dyes for Electrophoresis Tracking dyes are routinely used in both protein and nucleic acid electrophoresis to monitor the progress of electrophoresis and indicate at what point the gel should be stopped. Generally they are added to the sample prior to loading on to the gel (loading buffer). Alpha Laboratories offers a convenient ready-made DNA loading buffer. Triple Loading Buffer With three tracking dyes (Bromophenol Blue, Xylene Cyanol, and Orange G) Triple Dye Loading Buffer (6x) is a nondenaturing loading buffer for native polyacrylamide and agarose gel applications. In normal type agaroses in 0.5x TBE the dye mobility is broadly unaffected over the range of 0.5 to 1.4% w/v. The dyes run as follows (linear double stranded DNA); Orange G 50bp Bromophenol blue 300bp Xylene cyanol 4kbp Triple dye loading buffer contains 50% (w/v) sucrose and 40mM Tris base in deionized, distilled water. Triple Loading Buffer Pack Size EL0084 Triple Loading Buffer 6x 1.2ml 30

31 DNA Markers 100bp and 1kb Ladders Two-Ready-to-use DNA Size Markers for Gel Electrophoresis. The 100bp ladder and 1kb ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crisp band patterns. The two markers require no additional preparation and contain bromophenol blue for ease of use. Both markers are supplied at a concentration of 50µg/500µl (0.1µg/µl). DNA Marker Ladder Pack Size EL bp Ladder, 100-2,000bp each EL3010 1kb Ladder, ,000bp each Ultra Pure Electrophoresis Reagents Here Alpha Laboratories details a range of reagents commonly used in the preparation of electrophoresis and blotting buffers and solutions. These are all high purity reagents specifically formulated to be suitable for electrophoresis applications. SDS ULTRA PURE (Sodium-Dodecyl Sulphate) Used as a powerful protein denaturant and solubilising agent, which induces conformational changes in proteins at low concentrations, enabling proteins to be separated on the basis of their molecular weight. The purity of these products ensures that reproducible protein molecular weight measurements can be made with confidence. Purified to remove coloured contaminants that may interfere with spectrophotometric analysis. Zero free sulphates eliminate non-specific binding. Specifications: Molecular Weight Purity 99% OD280 (3% Solution) < 0.1 C12 Sulphate 99% Lead (Pb) < % Phosphate (PO4) < % Tris Buffer (Base) Tris(hydroxymethyl)aminomethane. Buffer salt used in both protein and DNA electrophoresis. Purified to remove ammonia and amine contaminants. Results in greater resolution during isoelectric focusing. Specifications: Molecular Weight Purity 99.9% Pb < 1 ppm OD280(1M solution) 0.15 Mg < 1 ppm Fe < 1 ppm As < 5 ppm Cu < 1 ppm Tris Buffer (Base) Pack Size EL0086 Tris Buffer (Base) 1kg Tween 20 Surfactant ULTRA PURE A Non-ionic surfactant, used for Western blotting and other molecular biology and biochemistry applications. Tween 20. Specially purified to remove epoxidized contaminants. Tween-20 is a Registered Trademark of ICI America. SDS ULTRA PURE (Sodium-Dodecyl Sulphate) Pack Size EL0070 Sodium Dodecyl Sulphate 1kg Tween 20 Surfactant ULTRA PURE Pack Size EL0091 Tween 20 Surfactant 1L 31

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