BioConfirm B Presentation

Transcription

1 BioConfirm B Presentation 1

2 Major Enhancements to Increase Your Productivity The Latest Version of our BioConfirm Product, B BioConfirm is protein and peptide characterization software using accurate mass LC/MS and MS/MS data from Agilent instruments. Major new features are: Usability and user experience improvements New disulfide bond mapping and scrambling workflow 2

3 BioConfirm B Offers the Lab Administrator BioConfirm B out of the box increases productivity of your lab! No need to create multiple methods or to manage sequence lists. Spend your time only reviewing samples that need your expert attention. With Automatic mass range detection Never miss proteins or their modifications or impurities No need to create a special method per Biomolecule or sample condition. Standalone Sequence Manager Organize your sequences at one central database Cached preprocessing results makes data analysis faster, increases throughput Easy Batch view Find the sample that needs review Red/ Green / Yellow flags Result review only mode for Biologist can restrict them from changing method parameters 3

4 BioConfirm B Offers the Biologist Tell BioConfirm B how you prepared your sample and collect your report! No need to understand Mass Spectrometry! Answer 4 questions about your sample Press the green button BioConfirm will tell you how well the protein was confirmed, along with reports and results 4

5 Simple and Clean UI for both: Lab Admin and Biologist BioConfirm B and previous versions were add on of MassHunter Qual. BioConfirm B is a separate application which works side by side with MassHunter Qual. There is also centralized Sequence Management by Sequence Manager application. 5

6 BioConfirm B Disulfide Bond Mapping Cysteine disulfide bonds - assist in maintaining structure of proteins - incorrect disulfide linkages can generate protein misfolding - important to identify location of these bonds and confirm that they are correct BioConfirm B workflow for: - Disulfide bond mapping - Disulfide bond scrambling 6

7 BioConfirm B Disulfide Bond Mapping 16 disulfide bonds total - 4 intra chain (light chain) - 8 intra chain (heavy chain) - 4 inter chain Sample prep is very important for this workflow as it can cause non-native scrambling of disulfide bonds 7

8 BioConfirm B Disulfide Bond Mapping 8

9 BioConfirm B Sample Comparison 9

10 BioConfirm B Deconvolution Mirror Plot Allows you to compare biosimilars or lot-to-lot variation 10

11 Sample-based Navigation 11

12 New BioConfirm tab in Acquisition Method Editor 12

13 Disulfide Bond Mapping Application Note P/N EN 13

14 ASMS 2016 Poster on Disulfide Bond Mapping BioConfirm B PS Training June

15 Summary Agilent offers a complete workflow for the characterization of intact proteins and peptide digests: Solid, reliable LC/MS hardware Dedicated consumables in the AdvanceBio columns line Integrated software from data acquisition through reporting Open access productivity with MassHunter Walkup that allows new users to be trained in five minutes New features have been added to BioConfirm B for enhanced usability and disulfide bond mapping 15

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