Roche Sequencing Story
|
|
- Elmer Bailey
- 6 years ago
- Views:
Transcription
1 Roche Sequencing Story Driving toward personalized medicine 9 November 2017 page 1 Roche For Research Use Only
2 IMPORTANT NOTICE Intended Use Unless explicitly stated otherwise, all Roche products and services referenced in this presentation/document are intended for the following use: For life science research only Not for use in diagnostic procedures All data on File For Roche Internal Use Only Do Not Distribute 9 November 2017 page 2 Roche
3 OUR GOAL Become the partner of choice for sequencing by making it simple and accessible enough for routine clinical use. Sample in. Result out. 9 November 2017 page 3 Roche For Research Use Only
4 Roche Sequencing Strategy PHASE I Invest in best-in-class technology across the NGS workflow PHASE II (TODAY) Create flexible, modular tools that simplify NGS workflow PHASE III Develop an end-to-end solution to enable diverse, routine clinical applications A phased approach to creating an end-to-end solution 9 November 2017 page 4 Roche For Research Use Only
5 The Roche Sequencing Vision An integrated NGS workflow SAMPLE PREPARATION SEQUENCING DATA, ANALYTICS AND REPORTING 9 November 2017 page 5 Roche For Research Use Only
6 Sequencing Vision Complete end to end solution: Sample in result out HEAT-Seq Sample Prep DNA Isolation Target Enrichment 2 nd and 3 rd Analysis Content: Oncology, Genetic Testing Roche Developed, Acquired or partnerships Library Preparation Sequencing Reporting 9 November 2017 page 6 Roche For Research Use Only
7 Sequencing solution investments Sample Prep Aug 2015 Kapa technology provides components essential to our fully integrated NGS solution. Kapa enzymes have the potential to improve the performance of the entire sequencing workflow and complement our existing technologies and expertise. MilliSect Nov 2014 High performance micro-dissection instrumentation, software and consumables promise to expand our diagnostic tool kit and bridge anatomic pathology (H&E staining) with genomic sequencing analysis through high quality sample preparation. AbVitro and Lumora Sep 2014 and July 2015 AbVitro s primer extension-based target enrichment technology offers the potential to support NGS directly from blood or other biological samples. Lumora s Heat Elution technology recovers DNA from FFPE and other challenging clinical samples in minutes, rather than the hours required for current technologies. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 7 Roche
8 Sequencing solution investments Sample Prep Aug 2015 Kapa technology provides components essential to our fully integrated NGS solution. Kapa enzymes have the potential to improve the performance of the entire sequencing workflow and complement our existing technologies and expertise. MilliSect Nov 2014 High performance micro-dissection instrumentation, software and consumables promise to expand our diagnostic tool kit and bridge anatomic pathology (H&E staining) with genomic sequencing analysis through high quality sample preparation. AbVitro and Lumora Sep 2014 and July 2015 AbVitro s primer extension-based target enrichment technology offers the potential to support NGS directly from blood or other biological samples. Lumora s Heat Elution technology recovers DNA from FFPE and other challenging clinical samples in minutes, rather than the hours required for current technologies. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 8 Roche
9 KAPA Library Preparation Kits and Enzymes Directed Evolution yields improved enzymes Core Technology Directed Evolution: A method of protein engineering that simulates natural selection in the lab Result Generates higher fidelity enzymes that reduce error and bias: Next-Gen Sequencing Library Preparation PCR/qPCR For Roche Internal Use Only Do Not Distribute 9 November 2017 page 9 Roche
10 Kit Selection by Application Application/ Sample Type Whole-genome Sequencing (human) Whole-genome Sequencing (small genomes) Exome Sequencing Amplicon Sequencing ChIP-Seq Methyl-Seq FFPE DNA Cell-free DNA Whole Transcriptome mrna-seq Non-coding RNA FFPE RNA Targeted RNA-Seq Ion Torrent Sequencing For further information on these applications, please refer to the individual sections or contact Global Customer Support at For Research Use Only. Not for use in diagnostic procedures. Data on file page 10 Roche
11 RNA LIBRARY PREPARATION SOLUTIONS
12 KAPA Library Prep Kits for RNA-Seq No RNA Enrichment HyperPrep HyperPlus cdna Library Prep Workflow Standard Streamlined Standard Streamlined Standard Streamlined KAPA Product KAPA Stranded RNA-Seq Kits KAPA RNA HyperPrep Kits KAPA Stranded mrna-seq Kits KAPA mrna HyperPrep Kits KAPA Stranded RNA-Seq Kits with RiboErase KAPA RNA HyperPrep Kits with RiboErase Library Prep Workflow time 6 8 hr 4 hr 8 10 hr 5.5 hr hr 6.5 hr Input Amount ng into library prep ng into library prep 100 ng 4 μg into mrna capture 50 ng 1 μg into mrna capture 100 ng 1 μg into rrna depletion 25 ng 1 μg into rrna depletion Sample Type High-quality Total RNA Degraded or FFPE Total RNA Enriched RNA High-quality Total RNA High-quality Total RNA Degraded or FFPE Total RNA Species Eukaryotic (Animal, Plant, etc.) Prokaryotic (Bacterial, etc.) Eukaryotic (Animal, Plant, etc.) Human, Mouse, and Rat Differentiating Applications Targeted RNA-Seq Whole Transcriptome mrna-seq Non-coding RNA Whole Transcriptome Shared Applications Strand-specific Automation-friendly Gene Expression Analysis; Detection of Gene Fusions, Isoforms, and other Structural Variants; Novel Transcript Identification; SNV Discovery Yes Yes For Research Use Only. Not for use in diagnostic procedures. page 12 Roche
13 The Evolution of KAPA Library Prep for RNA-Seq PERFORMANCE and WORKFLOW KAPA RNA HyperPrep Input (RiboErase) = 25 ng 1 µg Input (mrna capture) = 50 ng 1 µg Input (no enrichment) = 1 ng 100 ng KAPA Stranded RNA-Seq with RiboErase (HMR) Input = 100 ng 1 µg KAPA Stranded (m)rna-seq Input = 400 ng 1 µg for mrna capture, or ng for no capture October 2016 Gene expression analysis Detection of gene fusions, isoforms, and other structural variants Novel transcript identification SNV discovery
14 The Evolution of KAPA Library Prep for RNA-Seq PERFORMANCE and WORKFLOW KAPA RNA HyperPrep Input (RiboErase) = 25 ng 1 µg Input (mrna capture) = 50 ng 1 µg Input (no enrichment) = 1 ng 100 ng KAPA Stranded RNA-Seq with RiboErase (HMR) Input = 100 ng 1 µg KAPA Stranded (m)rna-seq Input = 400 ng 1 µg for mrna capture, or ng for no capture October 2016 Gene expression analysis Detection of gene fusions, isoforms, and other structural variants Novel transcript identification SNV discovery
15 The Evolution of KAPA Library Prep for RNA-Seq PERFORMANCE and WORKFLOW KAPA RNA HyperPrep Input (RiboErase) = 25 ng 1 µg Input (mrna capture) = 50 ng 1 µg Input (no enrichment) = 1 ng 100 ng KAPA Stranded RNA-Seq with RiboErase (HMR) Input = 100 ng 1 µg KAPA Stranded (m)rna-seq Input = 400 ng 1 µg for mrna capture, or ng for no capture October 2016 Gene expression analysis Detection of gene fusions, isoforms, and other structural variants Novel transcript identification SNV discovery
16 RNA-Seq Workflow Streamlining KAPA Stranded RNA Library Prep KAPA RNA HyperPrep mrna capture rrna depletion mrna capture rrna depletion Frag + Prime RNA Frag + Prime RNA 1st Strand Synthesis 1st Strand Synthesis 2nd Strand Synthesis Cleanup A-Tail Adapter Ligation Cleanup Cleanup 2.5 h 2nd Strand Synthesis & A-Tailing Adapter Ligation Cleanup Cleanup Amplification with PCR Mix 40 m Amplification with PCR Mix Cleanup Cleanup
17 RNA-Seq data expectations RiboErase Intergenic Intronic Exonic Ribosomal High quality Total RNA-seq Total RNA-seq depleted in silico mrna capture rrna depletion Low quality For Roche Internal Use Only Do Not Distribute 9 November 2017 page 17 Roche
18 KAPA mrna Hyper Sequencing of mrna-enriched samples provides a focused view of the exonic regions in the transcriptome Benefits include: Focused sequencing of protein-coding transcripts Improved coverage of gc-rich and low abundance transcripts Identification of an increased number of transcripts and genes Starting input 50 ng 1 ug For Research Use Only. Not for use in diagnostic procedures. Data on file 9 November 2017 page 18 Roche
19 Performance: Sequencing metrics Better utilize sequencing capacity. Libraries were generated in quadruplicate with 50 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers standard recommendations per workflow, where possible. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 19 Roche
20 Performance: GC-rich coverage KAPA mrna HyperPrep TruSeq Stranded mrna NEBNext Ultra Directional with mrna Capture Improved coverage. IGV coverage and splice junction tracks of the GC-rich GC-rich SLC2A4RG gene. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers standard recommendations per workflow, where possible. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 20 Roche
21 KAPA RNA Hyper with RiboErase Sequencing of total RNA samples that have been ribosomal RNA (rrna) depleted provides a more comprehensive representation of the transcriptome Benefits include: Up to 99.98% rrna depletion from various sample types Compatible with degraded inputs, including FFPE Detection of noncoding and precursor transcripts Improved coverage of GC-rich and low abundance transcripts Qualified automation method For Research Use Only. Not for use in diagnostic procedures. Data on file 9 November 2017 page 21 Roche
22 Impact of sample quality on library construction Sample quality dramatically affects library preparation Useful metrics to assess RNA quality include RNA Integrity Number (RIN) DV200 High-quality UHR FFPE 1: HQ FFPE FFPE 2: LQ FFPE Quality assessment of input RNA. Electropherograms were generated using an Agilent RNA 6000 Pico Kit. Blue shading highlights RNA fragments >200 nt. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 22 Roche
23 Impact of sample quality on library construction 100 Post-Ligation yield (pm) Adapter dimer (%) DV200 (%) High-quality UHR FFPE 1: HQ FFPE FFPE 2: LQ FFPE Sample quality affects RNA-seq library construction. Libraries were constructed using 100 ng of RNA from FFPE tissues (DV200 range 6 52%) and a high-quality UHR control (DV200 95%). Post-ligation yield was measured by qpcr and adapter dimer rates were calculated from electrophoretic assessment of final libraries. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 23 Roche
24 Impact of sample quality on library construction 100 Post-Ligation yield (pm) Adapter dimer (%) DV200 (%) Sample quality affects RNA-seq library construction. Libraries were constructed using 100 ng of RNA from FFPE tissues (DV200 range 6 52%) and a high-quality UHR control (DV200 95%). Post-ligation yield was measured by qpcr and adapter dimer rates were calculated from electrophoretic assessment of final libraries. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 24 Roche
25 Impact of sample quality on library construction 100 Post-Ligation yield (pm) Adapter dimer (%) LQ FFPE HQ FFPE DV200 (%) LQ FFPE HQ FFPE Sample quality affects RNA-seq library construction. Libraries were constructed using 100 ng of RNA from FFPE tissues (DV200 range 6 52%) and a high-quality UHR control (DV200 95%). Post-ligation yield was measured by qpcr and adapter dimer rates were calculated from electrophoretic assessment of final libraries. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 25 Roche
26 Impact of sample quality on sequencing metrics Sample quality affects some sequencing metrics. Libraries were constructed using 25 or 100 ng of UHR or FFPE RNA using RNA HyperPrep with RiboErase. Duplicate libraries were sequenced and subsampled to 14M reads. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 26 Roche
27 DNA LIBRARY PREPARATION SOLUTIONS
28 KAPA DNA Library Prep Kits HTP/LTP HyperPrep HyperPlus Applications Primary applications WGS, WES, Custom Panels, ChIP-Seq, Methyl-Seq WGS, WES, Custom Panels, ChIP-Seq, Methyl-Seq WGS. WES, Custom Panels Input 10 ng 5 μg 1 ng 1 μg 1 ng 1 μg High-quality DNA Recommended Recommended Recommended Sample Type FFPE DNA Compatible Recommended Recommended Cell-free DNA Compatible Recommended N/A ChIP DNA Compatible Recommended Compatible Performance Maximum Conversion Rate (Library Prep Efficiency) 40% 60% 100% DNA Polymerase KAPA HiFi KAPA HiFi KAPA HiFi Amplification Low-bias Amplification Yes Yes Yes PCR-free Workflows Compatible Recommended Recommended Fragmentation Mechanical Mechanical Integrated enzymatic Workflow Library Prep Time hr 2 3 hr hr (incl. fragmentation) Automation Friendly Yes Yes Yes For Research Use Only. Not for use in diagnostic procedures. page 28 Roche
29 Evolution of conversion rates Maximum conversion rate for 1 µg high quality input % Baseline KAPA Library Preparation Kit KAPA with bead Library Prep Kit 9 November 2017 page 29 Roche For Research Use Only KAPA Hyper Prep Data on file. For Research Use Only. Not for use in diagnostic procedures. KAPA HyperPlus With Fragmentation
30 KAPA HyperPlus: Fast & fully automatable KAPA with-bead Library Preparation KAPA Hyper Prep KAPA HyperPlus min 30 min 15 min 30 min 30 min 30 min 2 3 h h h Data on file. For Research Use Only. Not for use in diagnostic procedures. 9 November 2017 page 30 Roche For Research Use Only
31 Bringing Together Best-in-Class Technologies to Craft an End-to-End Solution A global effort, headquartered in Pleasanton, California SAMPLE PREPARATION SEQUENCING ANALYTICS MEDICAL CONTENT AvanSci Bio: Tissue Dissection Lumora: Heat Elution Kapa Biosystems: Library Prep NimbleGen: Target Enrichment AbVitro: PETE Genia: Single Molecule Nanopore Technology Stratos (research collaboration): Sequencing by Expansion Bina: Bioinformatics For Research Use Only. Not for use in diagnostic procedures. Ariosa (NIPT) Signature (Oncology) CAPP Medical (Oncology)
32 What is HyperCap? Integrated workflow of NGS Library Prep and Enrichment Solutions For Research Use Only. Not for use in diagnostic procedures.
33 Why use HyperCap? Streamlined workflow offering improved performance, flexibility, and automation-friendly Fast protocol Library prep in less than half a day Flexibility to perform shorter hybe supported High - efficiency library construction High conversion rates (yields) increase molecular complexity prior to capture Low fragmentation and amplification bias Versatile performance Improved enrichment metrics with across designs Comparable metrics between O/N and 4-hour hybridization Automation-friendly Steps that require user intervention or off-deck equipment can be eliminated For Research Use Only. Not for use in diagnostic procedures.
34 SeqCap EZ HyperCap Workflow v1.0 Reduce library prep time with Hyper Prep & HyperPlus kits KAPA Standard KAPA Hyper Prep KAPA HyperPlus Covaris Shearing * Covaris Shearing * Enzymatic Fragmentation End Repair SPRI Cleanup Single Tube End Repair & A-Tailing Adapter Ligation Single Tube End Repair & A-Tailing Adapter Ligation A-Tailing SPRI Cleanup SPRI Cleanup SPRI Cleanup Size Selection Size Selection Adapter Ligation PCR PCR SPRI Cleanup SPRI Cleanup SPRI Cleanup Size Selection PCR SPRI Cleanup ~3 hours* ~3 hours * Covaris shearing TAT is dependent on instrument, make, model and batch size. Time estimates for Standard and Hyper do not include covaris shearing. Enzymatic fragmentation allows for efficient and effective fragmentation for automated / high-throughput customers ~6 hours* For Roche Internal Use Do Distribute 9 November 2017 page 34 Roche For Research Use Only. Not for use in diagnostic procedures.
35 Target Enrichment Systems
36 Why perform targeted sequencing? Enable selective studying from relevant regions For Roche Internal Use Only Do Not Distribute 9 November 2017 page 36 Roche
37 SeqCap EZ Probe Library superior design More probes, uniform coverage, better capture Simple Tiling Design Target Region 120mer probes/baits tiled across the region. NimbleGen s Sequence Capture Design Target Region Up to 2,100,000 (50-105mer) probes selected for the region using special algorithm. Significantly more probes. Benefits: Higher density tiling Redundancy to reduce risk of unbalanced coverage or missed regions Ability to move probes for better uniformity For Roche Internal Use Only Do Not Distribute 9 November 2017 page 37 Roche
38 SeqCap EZ Choice Probe Design More probes significantly reduce risk of missing regions Simple Tiling Design Small insertion X Target Region Missing Region Target Region Missing Region X Missing or non-performing Probe NimbleGen Sequence Capture Design Small insertion Target Region X Target Region X Flanking probes can capture novel variants Redundant probes reduce risks For Roche Internal Use Only Do Not Distribute 9 November 2017 page 38 Roche
39 SeqCap EZ Library Workflow Capture targets up to 200 Mb with 2.1 M oligos in solution Target Regions Prepare with Next- Gen Sequencing Adaptors SeqCap EZ Probes (Solution Capture) Amplify DNA and Enrichment QC Sequence DNA on a Next-Gen Sequencer Genomic DNA Library Preparation Hybridization Capture and Washing Amplification and QC Sequencing For Roche Internal Use Only Do Not Distribute 9 November 2017 page 39 Roche
40 Comprehensive Variant Detection enabled by NimbleGen target enrichment technology gdna Seq SeqCap EZ Epigenetic variation detection (methyl seq) SeqCap Epi Sensitive, accurate and reproducible detection of variants RNA Seq SeqCap RNA For Roche Internal Use Only Do Not Distribute 9 November 2017 page 40 Roche
41 DNA Methylation Fields of Study From development to disease DNA Methylation is an essential field of genomics research with growing importance in human health and agriculture research. N NH 2 DNMTs N NH 2 CH 3 Cancer and carcinogenesis Autoimmune disease Mammalian embryonic development Neurological disorders O N AdoMet cytosine O AdoHcy N 5-methylcytosine For Roche Internal Use Only Do Not Distribute 9 November 2017 page 41 Roche
42 5mC monitoring technology Bisulfite mediated deamination of Cytosine Unmethylated : Methylated: Bisulfite is the gold standard technology for single-base resolution Uracil is replaced with thymine by DNA polymerase during amplification (T read as unmethylated C) BOTH 5mC and 5hmC are substantially resistant to the mutagenesis conditioned by HSO 3 - C read as either 5mC or 5hmC Destroys ~96% of the sample via acid-hydrolysis (nicks DNA) Clark SJ et al., Nucl. Acids Res (15): doi: /nar/ For Roche Internal Use Only Do Not Distribute 9 November 2017 page 42 Roche
43 Existing Technologies in DNA Methylation Studies One technology from discovery to validation ,000,000 s # of Samples Possible WGBS RRBS Microarrays SeqCap MassArray Amplicons Epi # of CpG s interrogated 1000 s qpcr 1-10 For Roche Internal Use Only Do Not Distribute 9 November 2017 page 43 Roche
44 SeqCap Epi Enrichment System Workflow Bisulfite treatment and then capture Target Regions Prepare a SeqCap Epi Library Bisulfite Conversion SeqCap Epi - Solution Capture (probes target CG, CHG, CHH) Amplify DNA Sequence HiSeq CpG Island CpG Island CpG Island Genomic DNA Library Preparation Chemical Mutagenesis Hybridization Capture and Washing Amplification and QC Sequencing With methylated adapters (C>T conversion) and amplification with uracil tolerant polymerase Focus bisulfite sequencing to ANY C of interest (Convert then Capture) For Roche Internal Use Only Do Not Distribute 9 November 2017 page 44 Roche
45 Workflows Considered for SeqCap Epi SeqCap Epi Alternative MethylSeq Library Prep Bisulfite Conversion Pre-Capture Amplify Library Capture Post-Capture Amplify Library Sequence MethylSeq Library Prep Capture Bisulfite Conversion Post-Capture Amplify Library Sequence 9 November 2017 page 45 Roche For Research Use Only
46 TRM1 TRM2 Capture Before Bisulfite Conversion Leads to More Severe Bottlenecking of Genomic Information 100% input complexity - SeqCap Epi Alternative 100% input complexity - - ± - ± MethylSeq Library Prep Bisulfite Conversion Pre-Capture Amplify Library Capture MethylSeq Library Prep Capture Bisulfite Conversion - - ± tighter bottleneck Post-Capture Amplify Library Sequence Post-Capture Amplify Library Sequence 9 November 2017 page 46 Roche For Research Use Only
47 Slide 46 TRM1 TRM2 changed the Seqcap epi to blue and the "before" in the title to orange Teri Rambo Mueller, 5/12/2016 changed the Seqcap epi to blue and the "before" in the title to orange Teri Rambo Mueller, 5/12/2016
48 TRM3 Capture Before Bisulfite Conversion Leads to More Severe Bottlenecking of Genomic Information SeqCap Epi Alternative + Higher output complexity Bisulfite Conversion + Lower sample input required Pre-Capture Amplify Library + Higher reproducibility - Higher probe density required - Lower output complexity - Higher sample input required Capture - Lower reproducibility Bisulfite Conversion + Lower probe density required 9 November 2017 page 47 Roche For Research Use Only
49 Slide 47 TRM3 changed the Seqcap epi to blue and the "before" in the title to orange Teri Rambo Mueller, 5/12/2016
50 Innovative Probe Design and Manufacture High probe density facilitates the capture of complex intermediate methylation patterns All possible methylation patterns represented in the bisulfite mutagenized sequencing library do not have to be specifically targeted by perfectly complementary probes: Sufficient misalignments are tolerated to allow capture by similar probes in a pool of millions Epi is designed to be used with ~200bp insert libraries, so targets with intermediate methylation can also be captured by adjacent capture probes up to 150 bp away High tiling density means that each CpG can be targeted by multiple probes For Roche Internal Use Only Do Not Distribute 9 November 2017 page 48 Roche
51 Existing Technologies in DNA Methylation Studies Limitations and gaps Whole Genome Whole Genome Bisulfite sequencing (WGBS) Microarrays Content Performance Cost Content Performance Limitations Poor depth of coverage Difficult data analysis Cost-prohibitive Indirect measurement Limited SNP calling on CpG Closed data analysis system Target Enrichment Agilent SureSelect Methyl-Seq Reduced Representation Bisfulite Sequencing (RRBS) Content Performance Content Convenience Cost Limited molecular complexity High PCR duplication Very high sample input Targets only one strand No custom content Fixed content limited by enzyme sites Missing data For Roche Internal Use Only Do Not Distribute 9 November 2017 page 49 Roche
52 Existing Technologies in DNA Methylation Studies Limitations and gaps Whole Genome Whole Genome Bisulfite sequencing (WGBS) Microarrays Content Performance Cost Content Performance Limitations Poor depth of coverage Difficult data analysis Cost-prohibitive Indirect measurement Limited SNP calling on CpG Closed data analysis system Target Enrichment Agilent SureSelect Methyl-Seq Reduced Representation Bisfulite Sequencing (RRBS) Content Performance Content Convenience Cost Limited molecular complexity High PCR duplication Very high sample input Targets only one strand No custom content Fixed content limited by enzyme sites Missing data For Roche Internal Use Only Do Not Distribute 9 November 2017 page 50 Roche
53 Compare with Whole Genome Bisulfite Sequencing High Correlation with WGBS Data R = Left-right symmetry indicates little systematic bias in % methylation measurements
54 Product Details SeqCap Epi Enrichment System Fixed Content SeqCap Epi CpGiant Enrichment Kit Epigenome-wide fixed design containing Illumina s Infinium Human Methylation450 BeadChip sites with smart padding Covers >5.5 million CpG sites in human genome Custom Content SeqCap Epi Choice Enrichment Kits Human Designs Small <30Mb, Medium 30-60Mb, and Large 60-90Mb designs SeqCap Epi Developer Enrichment Kits Non-Human and Large Human Designs Small <30Mb, Medium 30-60Mb, and Large 60-90Mb, and XL Mb designs For Roche Internal Use Only Do Not Distribute 9 November 2017 page 52 Roche
55 Existing Technologies in DNA Methylation Studies Limitations and gaps Whole Genome Whole Genome Bisulfite sequencing (WGBS) Microarrays Content Performance Cost Content Performance Limitations Poor depth of coverage Difficult data analysis Cost-prohibitive Indirect measurement Limited SNP calling on CpG Closed data analysis system Target Enrichment Agilent SureSelect Methyl-Seq Reduced Representation Bisulfite Sequencing (RRBS) Content Performance Content Convenience Cost Limited molecular complexity High PCR duplication Very high sample input Targets only one strand No custom content Fixed content limited by enzyme sites Missing data For Roche Internal Use Only Do Not Distribute 9 November 2017 page 53 Roche
56 SeqCap Epi CpGiant Enrichment Kits Upgrade your research from microarrays Epigenome-wide fixed design containing Illumina s Infinium Human Methylation450 BeadChip sites with smart padding to maximize CpG For Roche Internal Use Only Do Not Distribute 9 November 2017 page 54 Roche
57 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
58 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
59 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
60 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
61 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
62 The Importance of Targeting Watson and Crick SNPs are still important in the bisulfite world
63 Existing Technologies in DNA Methylation Studies Limitations and gaps Whole Genome Whole Genome Bisulfite sequencing (WGBS) Microarrays Content Performance Cost Content Performance Limitations Poor depth of coverage Difficult data analysis Cost-prohibitive Indirect measurement Limited SNP calling on CpG Closed data analysis system Target Enrichment Agilent SureSelect Methyl-Seq Reduced Representation Bisulfite Sequencing (RRBS) Content Performance Content Convenience Cost Limited molecular complexity High PCR duplication Very high sample input Targets only one strand No custom content Fixed content limited by enzyme sites Missing data For Roche Internal Use Only Do Not Distribute 9 November 2017 page 61 Roche
64 Direct Comparison The Importance of Targeting Watson and Crick Targeted Region Coverag e Targeted Region Coverage
65 SeqCap Epi Performance Benchmarking Head to head comparison with SureSelect Methyl-Seq workflow Red = Poor, Green = Good SAMPLE %DUP READS %ON TARGET READS MEAN DEPTH COV MEDIA N DEPTH COV PCT >1X PCT >10X PCT >20X PCT >50X PCT >100X FOLD 80 PENALT Y CpG POS COV 1X BOTH CpG POS COV 10X BOTH AVG STRAN D BIAS Roche Agilent-mimic design / 83.9 Mbp primary / Roche workflow Sample d to 55M reads Sample d to 55M reads IMR90_ IMR90_ NA12762_ NA12762_ NA12878_ NA12878_ Agilent design / 84.5 Mbp primary / Agilent workflow IMR90_ IMR90_ NA12762_ NA12762_ NA12878_ NA12878_ November 2017 page 63 Roche For Research Use Only
66 SeqCap Epi Performance Benchmarking Don t be concerned about lower probe specificity by targeting converted DNA Red = Poor, Green = Good %ON TARGET READS November 2017 page 64 Roche For Research Use Only
67 SeqCap Epi Performance Benchmarking ~25% Lower duplicate read rate Red = Poor, Green = Good %DUP READS November 2017 page 65 Roche For Research Use Only
68 SeqCap Epi Performance Benchmarking ~25 Fold better strand representation, infinitely better 5mC SNP error correction Red = Poor, Green = Good CpG POS COV 1X BOTH CpG POS COV 10X BOTH AVG STRAND BIAS November 2017 page 66 Roche For Research Use Only
69 TRM5 SeqCap Epi Brings Unique Advantages Coverage of both strands provides more SNP information Roche Nimblegen 44k Shared in Common 38k Agilent Design 11k SeqCap EPI call 65% more SNPs 9 November 2017 page 67 Roche For Research Use Only
70 Slide 67 TRM5 changed the colors Teri Rambo Mueller, 5/12/2016
71 Existing Technologies in DNA Methylation Studies Limitations and gaps Whole Genome Whole Genome Bisulfite sequencing (WGBS) Microarrays Content Performance Cost Content Performance Limitations Poor depth of coverage Difficult data analysis Cost-prohibitive Indirect measurement Limited SNP calling on CpG Closed data analysis system Target Enrichment Agilent SureSelect Methyl-Seq Reduced Representation Bisulfite Sequencing (RRBS) Content Performance Content Convenience Cost Limited molecular complexity High PCR duplication Very high sample input Targets only one strand No custom content Fixed content limited by enzyme sites Missing data For Roche Internal Use Only Do Not Distribute 9 November 2017 page 68 Roche
72 Capture Your DMRs of Interest Custom designs allow for monitoring unique DMRs 3.2Mbp capture design targeting 500 genes promoters (Developed with John Greally at Albert Einstein School of Medicine) For Roche Internal Use Only Do Not Distribute 9 November 2017 page 69 Roche
73 SeqCap Epi Enrichment System Leveraging NimbleGen s probe design and manufacture The Challenge High Complexity Capture Targets: Bisulfite treatment effectively doubles genome size; many probes needed to enable capture after bisulfite Loss of Information: Bisulfite treatment and inefficient enrichment can result in loss of genomic information Ineffective and Unaffordable Solutions: Previous commercial offerings failed to offer performance, cost, effectiveness and customizability The Solution: SeqCap Epi Innovative Probe Design: Roche NimbleGen technology enables efficient production of millions of probes to capture epigenome complexity Unique Workflow: Bisulfite before capture and targeting both strands for enrichment conserves molecular complexity Custom & Fixed Content Available: First to offer both catalog and custom designs to significantly advance the research depth and throughput For Roche Internal Use Only Do Not Distribute 9 November 2017 page 70 Roche
74 Capture Your Regions of Interest SeqCap Epi Enrichment Kits Products Design Size SeqCap Epi CpGiant Enrichment Kit 84 Mb SeqCap Epi Choice Enrichment Kit Small (<30Mb) Medium (30-60Mb) Large (60-90Mb) SeqCap Epi Developer Enrichment Kit Small (<30Mb) Medium (30-60Mb) Large (60-90Mb) XL (90-210Mb) Up to 90 Mb Up to 210 Mb For Roche Internal Use Only Do Not Distribute 9 November 2017 page 71 Roche
75 SeqCap Epi CpGiant Demo Data Review design Regions of interest selection An epigenome-wide fixed content design was created containing all of the Illumina Infinium HumanMethylation 450 BeadChip sites with smart padding to maximize the number of CpG s that will be assayed (5.5 million). 80Mb design Innovative probe design and manufacture allows for the capture of both strands enabling the detection of complex or rare methylation events. 9 November 2017 page 72 Roche For Research Use Only
76 SeqCap Epi CpGiant Demo Data Review experimental design 1 ug NA12891 and NA19240 sample DNA with LTP/HTP Kapa Library Prep Kit SeqCap Epi User's Guide v1.2 1 samples pre-captured multiplexed 72 hour hybridization Illumina HiSeq 2000 in high output mode, v3 chemistry and 2x101bp reads 9 November 2017 page 73 Roche For Research Use Only
77 SeqCap Epi CpGiant Demo Data Review analysis Sequencing reads in fastq files Examine Sequence Read Quality FastQC Adapter and Quality Trimming Trimmomatic Map Reads to Reference Genome BS Map Split Reads by Strand, Remove Duplicates and Merge Split Files Bamtools, SamTools, Picard Mark Duplicates SeqCap Epi Data Analysis Tech Note Filter For Properly Paired Reads and Clip Overlapping Reads Methylation Analysis BSMAP, BisSNP 9 November 2017 page 74 Roche For Research Use Only
78 SeqCap Epi CpGiant Demo Data Review performance % dup rate % on-target Mean Depth of Coverage % bases >= 1 % bases >= 10 NA NA NA NA Fold 80 base penalty(1/nc80) = 2.27(average for the 4 samples) The amount of additional sequencing needed to bring 80% of bases to the mean coverage. 9 November 2017 page 75 Roche For Research Use Only
79 Demo Data Packages available now! Product rawdata bamfiles README Gene List Tech note metrics bedfiles SeqCap Epi CP Giant x x x NA x x x Provides you with an easy, low resource way to evaluate our products. Gives you a preview into our performance and our method of data analysis. Helps you make a good decision on which exome or workflow to choose. Gets your Bioinformaticist involved early. 9 November 2017 page 76 Roche For Research Use Only
80 Other Demo Data Packages DPOP Package SeqCap EZ Exome V3-1 day hybe SeqCap EZ Exome V3-3 day hybe SeqCap EZ Exome V3 + UTR SeqCap EZ MedExome and MedExome + Mito SeqCap EZ Mitome SeqCap EZ MedExome - post-capture multiplexed SeqCap EZ MedExome - Hyper v1.0 SeqCap EZ MedExome - HyperPlus v1.0 SeqCap EZ MedExome - Whole Blood HyperPlus v1.0 SeqCap EZ Inherited Disease Panel SeqCap RNA - lnc RNA SeqCap RNA - Choice VCRome - 1 day hybe VCRome - 3 day hybe SeqCap Epi CP Giant SeqCap EZ Comprehensive Cancer Panel - 1 day hybe SeqCap EZ Comprehensive Cancer Panel - 3 day hybe SeqCap EZ Comprehensive Cancer Panel - FFPE HyperPlus 4 hour v1.0 SeqCap EZ Comprehensive Cancer Panel - HyperPlus v1.0 SeqCap EZ Comprehensive Cancer Panel - Hyper v1.0 SeqCap EZ Comprehensive Cancer Panel - HyperPlus Automation v1.0 HEAT-Seq Oncology Panel HEAT-Seq Choice - CFTR HEAT-Seq Ultra Oncology Hot Spot Panel - NA12878 HEAT-Seq Ultra Oncology Hot Spot Panel - TruQ4 HEAT-Seq Ultra Choice - XXL - Pediatric Tumor SeqCap EZ Exome Design - Neurology SeqCap EZ Exome Design - Soy SeqCap EZ Exome Design - UTR only SeqCap EZ Exome V2 BED Files ONLY SeqCap Epi Design - Human Disease Methylome SeqCap EZ Exome Design - Barley SeqCap EZ Exome Design - Human MHC SeqCap EZ Exome Design - Maize SeqCap EZ Exome Design - Mouse SeqCap EZ Exome Design - Pig SeqCap EZ Exome Design - Switchgrass SeqCap EZ Exome Design - Wheat SeqCap EZ Exome V1 SeqCap EZ Exome Design - Canine DPOP Package includes: Raw data, BAM files (for most), Gene Lists, Metrics, BED files, README and Data Analysis Technical Note 9 November 2017 page 77 Roche For Research Use Only
81 Discover More, Sequence Less Comprehensive solutions with exome & custom designs SeqCap EZ System Products Design Size SeqCap Epi System Products Design Size EXOME SeqCap Epi CPGiant 84 Mb SeqCap EZ Medexome 47 Mb SeqCap Epi Choice up to 90 Mb SeqCap EZ Medexome Plus up to 200 Mb SeqCap Epi Developer up to 210 Mb SeqCap EZ Exome Library Mb Human Disease Methylome Design 156 Mb SeqCap EZ Exome Library Mb SeqCap EZ Exome + UTR 96 Mb SeqCap RNA System Products Design Size SeqCap EZ Exome Plus up to 200 Mb SeqCap RNA Human lncrna 17 Mb HGSC VCRome 45.2 Mb SeqCap RNA Choice up to 7 Mb CUSTOM SeqCap RNA Choice XL Mb SeqCap EZ Choice up to 7 Mb SeqCap RNA Developer up to 200 Mb SeqCap EZ Choice XL up to 200 Mb SeqCap EZ Developer up to 200 Mb HEAT-Seq System Products Design Size DESIGNS HEAT-Seq Oncology Panel 60 genes Human Oncology Panel 2.75 Mb (981 genes) HEAT-Seq Ultra Oncology Hotspot Panel 53 genes Comprehensive Cancer 4 Mb (578 genes) HEAT-Seq Choice >5000 genes Neurology Panel 1.5 Mb (256 genes) HEAT-Seq Ultra Choice up to 60 genes Human 50 Mb UTR Human MHC Mitochondria Genome Mouse Exome Pig Exome Soy Exome Maize Exome Wheat Exome Barley Exome Canine Exome Switchgrass Exome 50 Mb 4.97 Mb 16 Kb 54.3 Mb 58.1 Mb 85.3 Mb 110 Mb Mb 88.6 Mb 152 Mb 50 Mb For Roche Internal Use Only Do Not Distribute 9 November 2017 page 78 Roche
82 SEQUENCING Nanopore Technology A semiconductor-based sequencing platform with potential to address the diversity of clinical NGS requirements IMPORTANCE Potential for disruptive technology with high accuracy and fast turnaround Single molecule sequencing (SMS) for increased sensitivity and enhanced structural variant detection Compact format integrates easily into laboratory space Product in development and not commercially available 9 November 2017 page 79 Roche For Research Use Only
83 Sequencing solution investments Menu & data solutions, contd. Ariosa Diagnostics Dec 2014 Testing service provides highly targeted and accurate Harmony non-invasive prenatal test* (NIPT) in a CLIAcertified laboratory using cell-free DNA technology. The technology has the potential to provide early actionable information in pregnancy, cancer and transplantation, aligning with our personalized healthcare strategy. Bina Technologies Dec 2014 Informatics and data management are critical components of a seamless, end-to-end sequencing solution. Bina offers solutions to improve the efficiency and value of genomic analysis and continues to develop new methodologies and algorithms that link NGS data to disease-relevant genetic markers. * The Harmony Prenatal Test is developed by Ariosa Diagnostics. Ariosa Diagnostics is a laboratory certified under the Clinical Laboratory Improvement Amendments (CLIA). As with other laboratory-developed tests, this test has not been cleared or approved by the US FDA. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 80 Roche
84 Sequencing solution investments Menu & data solutions CAPP Medical Apr 2015 Focused, next-generation tumor sequencing assays using simple blood draws and cell free DNA (cfdna) technology have the potential to significantly shorten the time to cancer diagnosis and change routine cancer diagnostic monitoring. Signature Genomics Feb 2015 Translational oncology and genomics company offers large blood and tissue biobanks for multiple cancer types, constructed from multicenter prospective clinical studies. Samples are used to validate circulating cell free DNA (cfdna) NGS tests like that of CAPP Medical with retrospective clinical testing. For Roche Internal Use Only Do Not Distribute 9 November 2017 page 81 Roche
85 Sample Preparation Goals Become the leader in blood-based sequencing, including cell-free DNA Automate and optimize direct-from-sample sequencing Sequencing Goals Continue to seek and support innovation Informatics Goals DATA, ANALYTICS AND REPORTING Committed to Ensuring Success by Developing Disruptive Technology Develop, partner and acquire proprietary end-to-end data management, analytics and reporting 9 November 2017 page 82 Roche For Research Use Only
86 Bringing Together Best-in-Class Technologies to Craft an End-to-End Solution A global effort, headquartered in Pleasanton, Calif. SAMPLE PREPARATION SEQUENCING ANALYTICS MEDICAL CONTENT For presentation purposes only Not for distribution 9 November 2017 page Roche
87 Product Overview Circulating Tumor DNA (ctdna) Crowley, E. et al. (2013) Liquid biopsy: monitoring cancer-genetics in the blood Nat. Rev. Clin. Oncol. doi: /nrclinonc November 2017 page 84 Roche For Research Use Only
88 Product Overview End-to-end Workflow * *The NextSeq 500/550 instruments and associated sequencing reagents are manufactured and sold by lllumina and are not supplied by Roche. 9 November 2017 page 85 Roche For Research Use Only
89 Product Overview Panel Options Analyze genes in NCCN 1 Guidelines Analyze genes targeted in clinical trials Analyze genes in longitudinal studies # of Genes Size Mutation Classes Targeted Panel Expanded Panel Surveillance Panel genes 81kb 192kb 198kb SNVs, CNVs, Indels, Fusions NCCN = National Comprehensive Cancer Network 1 National Comprehensive Cancer Network, October 15, 2016 Data on file. 9 November 2017 page 86 Roche For Research Use Only
90 Product Overview AVENIO ctdna Targeted Kit SNVs Indels* Fusions** CNVs** Amplifications ALK KRAS ALK ALK EGFR APC MET APC RET ERBB2 BRAF NRAS BRAF ROS1 MET BRCA1 PDGFRA EGFR BRCA2 RET ERBB2 DPYD ROS1 KIT EGFR TP53 MET ERBB2 UGT1A1 KIT Genes with whole exon coverage in bold *Indels are limited to variants in a pre-specified list of positions, referred to as "Loci of Interest", except for EGFR exon 19 long deletions, EGFR exon 20 long insertions and MET long insertions, which are not restricted to a pre-defined set of Indels **Detection of Fusions and CNVs are limited to variants in a pre-specified list of positions, referred to as "Loci of Interest" in the AVENIO ctdna Analysis Software Includes select introns for fusion detection Example: Lung Cancer National Comprehensive Cancer Network EGFR ALK Future Targets KRAS MET BRAF ROS1 ERBB2 Source: ClinicalTrials.gov, NCCN.org, Data on file Includes genes in NCCN Guidelines 9 November 2017 page 87 Roche For Research Use Only
91 Analysis and Results Variant information Variant information 9 November 2017 page 88 Roche For Research Use Only
92 Recap Summary Analyze up to 16 samples at a time, using either fastq or bcl format 2 user profiles, 3 gene panels, 4 mutation classes Customizable workflows and annotations Separate reports for sample metrics and sample attributes Summary 9 November 2017 page 89 Roche For Research Use Only
93 Roche Diagnostics and Pharmaceutical Synergy Positioned for leadership in personalized healthcare DIAGNOSTICS PHARMACEUTICALS Combined strengths of Pharmaceuticals and Diagnostics Synergies in research, development and marketing Unique global network of alliances Best positioned to deliver on the promise of personalized healthcare Confidential. Do Not Distribute.
94 Roche Sequencing Enabling personalized medicine on a global scale
95
96 Doing now what patients need next For Roche Internal Use Only Do Not Distribute 9 November 2017 page 93 Roche
Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES
Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES Roche Sequencing Solutions Roche is helping to shape the future of personalized medicine by integrating best-in-class sequencing technologies
More informationDiscover More, Sequence Less with SeqCap Products. John C. Tan, Ph.D. Roche NimbleGen, Research & Development
Discover More, Sequence Less with SeqCap Products John C. Tan, Ph.D. Roche NimbleGen, Research & Development Roche NimbleGen s Target Enrichment Solutions Overview Why Targeted Sequencing Technology Overview
More informationDNA METHYLATION RESEARCH TOOLS
SeqCap Epi Enrichment System Revolutionize your epigenomic research DNA METHYLATION RESEARCH TOOLS Methylated DNA The SeqCap Epi System is a set of target enrichment tools for DNA methylation assessment
More informationRAPID, ROBUST & RELIABLE
Roche Sample Prep Solutions for RNA-Seq Sequence what matters RAPID, ROBUST & RELIABLE Sample P le Samp Quant ifi /QC tion ca As the first step in the NGS workflow continuum, sample prep holds the key
More informationComprehensive NGS Target Enrichment for Medical Research COUNTLESS OPTIONS EZ CHOICE. For Research Use Only. Not for use in diagnostic procedures.
Comprehensive NGS Target Enrichment for Medical Research COUNTLESS OPTIONS EZ CHOICE For Research Use Only. Not for use in diagnostic procedures. Specialized Tools for Specialized Research Contents Specialized
More informationACCEL-NGS 2S DNA LIBRARY KITS
ACCEL-NGS 2S DNA LIBRARY KITS Accel-NGS 2S DNA Library Kits produce high quality libraries with an all-inclusive, easy-to-use format. The kits contain all reagents necessary to build high complexity libraries
More informationG E N OM I C S S E RV I C ES
GENOMICS SERVICES ABOUT T H E N E W YOR K G E NOM E C E N T E R NYGC is an independent non-profit implementing advanced genomic research to improve diagnosis and treatment of serious diseases. Through
More informationAdmera Health RUO Services
Admera Health RUO Services Services 1. RNA-Seq... 2 2. Single-cell RNAseq (scrna-seq)... 3 3. Whole Exome Sequencing (WES)... 4 4. Whole Genome Sequencing (WGS)... 5 5. Whole Genome Bisulfite Sequencing
More informationWelcome to the NGS webinar series
Welcome to the NGS webinar series Webinar 1 NGS: Introduction to technology, and applications NGS Technology Webinar 2 Targeted NGS for Cancer Research NGS in cancer Webinar 3 NGS: Data analysis for genetic
More informationAgilent NGS Solutions : Addressing Today s Challenges
Agilent NGS Solutions : Addressing Today s Challenges Charmian Cher, Ph.D Director, Global Marketing Programs 1 10 years of Next-Gen Sequencing 2003 Completion of the Human Genome Project 2004 Pyrosequencing
More informationNovel methods for RNA and DNA- Seq analysis using SMART Technology. Andrew Farmer, D. Phil. Vice President, R&D Clontech Laboratories, Inc.
Novel methods for RNA and DNA- Seq analysis using SMART Technology Andrew Farmer, D. Phil. Vice President, R&D Clontech Laboratories, Inc. Agenda Enabling Single Cell RNA-Seq using SMART Technology SMART
More informationQIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd
QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd 1 Our current NGS & Bioinformatics Platform 2 Our NGS workflow and applications 3 QIAGEN s
More informationDNA METHYLATION NH 2 H 3. Solutions using bisulfite conversion and immunocapture Ideal for NGS, Sanger sequencing, Pyrosequencing, and qpcr
NH 2 DNA METHYLATION H 3 C Solutions using bisulfite conversion and immunocapture Ideal for NGS, Sanger sequencing, Pyrosequencing, and qpcr NH 2 N N H PAGE 3 Understanding DNA Methylation DNA methylation
More informationDeep Sequencing technologies
Deep Sequencing technologies Gabriela Salinas 30 October 2017 Transcriptome and Genome Analysis Laboratory http://www.uni-bc.gwdg.de/index.php?id=709 Microarray and Deep-Sequencing Core Facility University
More informationAPPLICATION NOTE
APPLICATION NOTE www.swiftbiosci.com NGS Library Preparation Produces Balanced, Comprehensive Methylome Coverage from Low Input Quantities ABSTRACT Next-generation sequencing (NGS) of bisulfite-converted
More informationGet to Know Your DNA. Every Single Fragment.
HaloPlex HS NGS Target Enrichment System Get to Know Your DNA. Every Single Fragment. High sensitivity detection of rare variants using molecular barcodes How Does Molecular Barcoding Work? HaloPlex HS
More informationSurely Better Target Enrichment from Sample to Sequencer
sureselect TARGET ENRICHMENT solutions Surely Better Target Enrichment from Sample to Sequencer Agilent s market leading SureSelect platform provides a complete portfolio of catalog to custom products,
More informationIntegrated NGS Sample Preparation Solutions for Limiting Amounts of RNA and DNA. March 2, Steven R. Kain, Ph.D. ABRF 2013
Integrated NGS Sample Preparation Solutions for Limiting Amounts of RNA and DNA March 2, 2013 Steven R. Kain, Ph.D. ABRF 2013 NuGEN s Core Technologies Selective Sequence Priming Nucleic Acid Amplification
More informationSample to Insight. Dr. Bhagyashree S. Birla NGS Field Application Scientist
Dr. Bhagyashree S. Birla NGS Field Application Scientist bhagyashree.birla@qiagen.com NGS spans a broad range of applications DNA Applications Human ID Liquid biopsy Biomarker discovery Inherited and somatic
More informationSurely Better Target Enrichment from Sample to Sequencer and Analysis
sureselect TARGET ENRIChment solutions Surely Better Target Enrichment from Sample to Sequencer and Analysis Agilent s market leading SureSelect platform provides a complete portfolio of catalog to custom
More informationNext Generation Sequencing. Target Enrichment
Next Generation Sequencing Target Enrichment Next Generation Sequencing Your Partner in Every Step from Sample to Data NGS: Revolutionizing Genetic Analysis with Single-Molecule Resolution Next generation
More informationSureSelect Target Enrichment for the Ion Proton TM Next Generation Sequencing System
SureSelect Target Enrichment for the Ion Proton TM Next Generation Sequencing System Demonstrated performance you can count on Christina Chiu Product Manager, SureSelect Kyeong Jeong Ph.D. R&D Scientist
More informationIncrease Sequencing Efficiency with the SeqCap EZ Prime Exome
Sequencing Solutions Technical Note June 2018 How To Increase Sequencing Efficiency with the SeqCap EZ Prime Exome Applications Whole Exome Sequencing Products SeqCap EZ Prime Exome SeqCap EZ HyperCap
More informationHigh Throughput Sequencing the Multi-Tool of Life Sciences. Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center
High Throughput Sequencing the Multi-Tool of Life Sciences Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center Complementary Approaches Illumina Still-imaging of clusters (~1000
More informationTECH NOTE Pushing the Limit: A Complete Solution for Generating Stranded RNA Seq Libraries from Picogram Inputs of Total Mammalian RNA
TECH NOTE Pushing the Limit: A Complete Solution for Generating Stranded RNA Seq Libraries from Picogram Inputs of Total Mammalian RNA Stranded, Illumina ready library construction in
More informationHigh-yield, Scalable Library Preparation with the NEBNext Ultra II FS DNA Library Prep Kit
be INSPIRED drive DISCOVERY stay GENUINE TECHNICAL NOTE High-yield, Scalable Library Preparation with the NEBNext Ultra II FS DNA Library Prep Kit Improving performance, ease of use and reliability of
More informationNew Frontiers of Genetic Profiling Achieve Higher Sensitivity and Greater Insights with Molecular Barcodes, Long Read Capture and Optimized Exomes
New Frontiers of Genetic Profiling Achieve Higher Sensitivity and Greater Insights with Molecular Barcodes, Long Read Capture and Optimized Exomes Jennifer Jones, PhD Senior Field Application Scientist
More informationWet-lab Considerations for Illumina data analysis
Wet-lab Considerations for Illumina data analysis Based on a presentation by Henriette O Geen Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center Complementary Approaches Illumina
More informationGene Regulation Solutions. Microarrays and Next-Generation Sequencing
Gene Regulation Solutions Microarrays and Next-Generation Sequencing Gene Regulation Solutions The Microarrays Advantage Microarrays Lead the Industry in: Comprehensive Content SurePrint G3 Human Gene
More informationHyperCap, an automatable workflow on the Agilent Bravo B
Automation Note February 2018 HyperCap, an automatable workflow on the Agilent Bravo B 1. OVERVIEW As the demand for next-generation sequencing (NGS) grows, laboratories must adapt to manage increased
More informationIntroduction Bioo Scientific
Next Generation Sequencing Catalog 2014-2015 Introduction Bioo Scientific Bioo Scientific is a global life science company headquartered in Austin, TX, committed to providing innovative products and superior
More informationIllumina s Suite of Targeted Resequencing Solutions
Illumina s Suite of Targeted Resequencing Solutions Colin Baron Sr. Product Manager Sequencing Applications 2011 Illumina, Inc. All rights reserved. Illumina, illuminadx, Solexa, Making Sense Out of Life,
More informationEVOLVE. Blue-footed booby (Sula nebouxii)
Next-Generation Solutions EVOLVE. Blue-footed booby (Sula nebouxii) TABLE OF CONTENTS Kit Selection by Application...................... iv Sample QC Solutions KAPA hgdna Quantification and QC Kits..............
More informationImpact of gdna Integrity on the Outcome of DNA Methylation Studies
Impact of gdna Integrity on the Outcome of DNA Methylation Studies Application Note Nucleic Acid Analysis Authors Emily Putnam, Keith Booher, and Xueguang Sun Zymo Research Corporation, Irvine, CA, USA
More informationHaloPlex HS. Get to Know Your DNA. Every Single Fragment. Kevin Poon, Ph.D.
HaloPlex HS Get to Know Your DNA. Every Single Fragment. Kevin Poon, Ph.D. Sr. Global Product Manager Diagnostics & Genomics Group Agilent Technologies For Research Use Only. Not for Use in Diagnostic
More informationIncorporating Molecular ID Technology. Accel-NGS 2S MID Indexing Kits
Incorporating Molecular ID Technology Accel-NGS 2S MID Indexing Kits Molecular Identifiers (MIDs) MIDs are indices used to label unique library molecules MIDs can assess duplicate molecules in sequencing
More informationIntroducing QIAseq. Accelerate your NGS performance through Sample to Insight solutions. Sample to Insight
Introducing QIAseq Accelerate your NGS performance through Sample to Insight solutions Sample to Insight From Sample to Insight let QIAGEN enhance your NGS-based research High-throughput next-generation
More informationSureSelect XT HS. Target Enrichment
SureSelect XT HS Target Enrichment What Is It? SureSelect XT HS joins the SureSelect library preparation reagent family as Agilent s highest sensitivity hybrid capture-based library prep and target enrichment
More informationObtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
be INSPIRED drive DISCOVERY stay GENINE TECHNICAL NOTE Directional rrna depletion Obtain superior NGS library performance with lower input amounts using the NEBNext ltra II Directional RNA Library Prep
More informationObtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
be INSPIRED drive DISCOVERY stay GENINE TECHNICAL NOTE Directional rrna depletion Obtain superior NGS library performance with lower input amounts using the NEBNext ltra II Directional RNA Library Prep
More informationLab methods: Exome / Genome. Ewart de Bruijn
Lab methods: Exome / Genome 27 06 2013 Ewart de Bruijn Library prep is only a small part of the complete DNA analysis workflow DNA isolation library prep enrichment flowchip prep sequencing bioinformatics
More informationcdna CaptureSeq Reveals unappreciated diversity of the transcriptome
www.nimblegen.com cdna CaptureSeq Reveals unappreciated diversity of the transcriptome J.A. Jeddeloh, Ph.D. Director, Business Development, Roche NimbleGen jeffrey.jeddeloh@roche.com For Life Science Research
More informationTECH NOTE Stranded NGS libraries from FFPE samples
TECH NOTE Stranded NGS libraries from FFPE samples Robust performance with extremely degraded FFPE RNA (DV 200 >25%) Consistent library quality across a range of input amounts (5 ng 50 ng) Compatibility
More informationThe New Genome Analyzer IIx Delivering more data, faster, and easier than ever before. Jeremy Preston, PhD Marketing Manager, Sequencing
The New Genome Analyzer IIx Delivering more data, faster, and easier than ever before Jeremy Preston, PhD Marketing Manager, Sequencing Illumina Genome Analyzer: a Paradigm Shift 2000x gain in efficiency
More informationDNA. bioinformatics. epigenetics methylation structural variation. custom. assembly. gene. tumor-normal. mendelian. BS-seq. prediction.
Epigenomics T TM activation SNP target ncrna validation metagenomics genetics private RRBS-seq de novo trio RIP-seq exome mendelian comparative genomics DNA NGS ChIP-seq bioinformatics assembly tumor-normal
More informationHigh Throughput Sequencing the Multi-Tool of Life Sciences. Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center
High Throughput Sequencing the Multi-Tool of Life Sciences Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center DNA Technologies & Expression Analysis Cores HT Sequencing (Illumina
More informationCancer Genetics Solutions
Cancer Genetics Solutions Cancer Genetics Solutions Pushing the Boundaries in Cancer Genetics Cancer is a formidable foe that presents significant challenges. The complexity of this disease can be daunting
More informationTranscriptomics analysis with RNA seq: an overview Frederik Coppens
Transcriptomics analysis with RNA seq: an overview Frederik Coppens Platforms Applications Analysis Quantification RNA content Platforms Platforms Short (few hundred bases) Long reads (multiple kilobases)
More informationAccessible answers. Targeted sequencing: accelerating and amplifying answers for oncology research
Accessible answers Targeted sequencing: accelerating and amplifying answers for oncology research Help advance precision medicine Accelerate results with Ion Torrent NGS Life without cancer. This is our
More informationApplication Note Selective transcript depletion
Application Note Selective transcript depletion Sample Authors Laura de Jager RED Scientist Michael Berry Bioinformatics Scientist Luke Esau RED Senior Scientist Ross Wadsworth RED Team Lead Roche Sequencing
More informationIon S5 and Ion S5 XL Systems
Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5
More informationAnalytical verification methods for the Oncomine Lung cfdna Assay using the Ion S5 XL System
WHITE PAPER Oncomine Lung cfdna Assay and Ion S5 XL System Analytical verification methods for the Oncomine Lung cfdna Assay using the Ion S5 XL System Key highlights Investigate tumor heterogeneity and
More informationYour Best Data: Teaming QIAGEN Chemistry & Bioinformatics to Drive Samples to Insight
Your Best Data: Teaming QIAGEN Chemistry & Bioinformatics to Drive Samples to Insight Aysel Heckel Director Clinical Solutions Sales Dr. Anne Arens Field Application Scientist Course on Variant Detection
More informationsolid S Y S T E M s e q u e n c i n g See the Difference Discover the Quality Genome
solid S Y S T E M s e q u e n c i n g See the Difference Discover the Quality Genome See the Difference With a commitment to your peace of mind, Life Technologies provides a portfolio of robust and scalable
More informationIon S5 and Ion S5 XL Systems
Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5
More informationGenome Resequencing. Rearrangements. SNPs, Indels CNVs. De novo genome Sequencing. Metagenomics. Exome Sequencing. RNA-seq Gene Expression
Genome Resequencing De novo genome Sequencing SNPs, Indels CNVs Rearrangements Metagenomics RNA-seq Gene Expression Splice Isoform Abundance High Throughput Short Read Sequencing: Illumina Exome Sequencing
More informationKAPA RNA HyperPrep Workflow: Recommendations and Expectations for RNA-sequencing Using Degraded Inputs
Application Note RNA-sequencing using degraded inputs KAPA RNA HyperPrep Workflow: Recommendations and Expectations for RNA-sequencing Using Degraded Inputs AUTHORS Nancy Nabilsi Senior Applications Scientist
More informationIntroduction to RNA-Seq. David Wood Winter School in Mathematics and Computational Biology July 1, 2013
Introduction to RNA-Seq David Wood Winter School in Mathematics and Computational Biology July 1, 2013 Abundance RNA is... Diverse Dynamic Central DNA rrna Epigenetics trna RNA mrna Time Protein Abundance
More informationComplete Sample to Analysis Solutions for DNA Methylation Discovery using Next Generation Sequencing
Complete Sample to Analysis Solutions for DNA Methylation Discovery using Next Generation Sequencing SureSelect Human/Mouse Methyl-Seq Kyeong Jeong PhD February 5, 2013 CAG EMEAI DGG/GSD/GFO Agilent Restricted
More informationTarget Enrichment Strategies for Next Generation Sequencing
Target Enrichment Strategies for Next Generation Sequencing Anuj Gupta, PhD Agilent Technologies, New Delhi Genotypic Conference, Sept 2014 NGS Timeline Information burst Nearly 30,000 human genomes sequenced
More informationFFPE in your NGS Study
FFPE in your NGS Study Richard Corbett Canada s Michael Smith Genome Sciences Centre Vancouver, British Columbia Dec 6, 2017 Our mandate is to advance knowledge about cancer and other diseases and to use
More informationSURESELECTXT LOW INPUT TARGET ENRICHMENT
SURESELECTXT LOW INPUT TARGET ENRICHMENT Low Input FFPE Optimized Streamlined Workflow SureSelect XT Low Input What is it? SureSelect XT Low Input is a low-input, FFPE-optimized library preparation kit.
More informationEnterprise Interest I am an employee of ThermoFisher Scientific.
Enterprise Interest I am an employee of ThermoFisher Scientific. Developing a multiplex next-generation sequencing assay to study highly clonal tumor samples Dumitru Brinza, Ph.D Clinical Next-Generation
More informationDNA concentration and purity were initially measured by NanoDrop 2000 and verified on Qubit 2.0 Fluorometer.
DNA Preparation and QC Extraction DNA was extracted from whole blood or flash frozen post-mortem tissue using a DNA mini kit (QIAmp #51104 and QIAmp#51404, respectively) following the manufacturer s recommendations.
More informationGENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS. Genomics Solutions Portfolio
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS Genomics Solutions Portfolio WORKFLOW SOLUTIONS FROM EXTRACTION TO ANALYSIS Application-based answers for every step of your workflow Scientists
More informationMatthew Tinning Australian Genome Research Facility. July 2012
Next-Generation Sequencing: an overview of technologies and applications Matthew Tinning Australian Genome Research Facility July 2012 History of Sequencing Where have we been? 1869 Discovery of DNA 1909
More informationNGS: Digital RNAseq & Library Prep Seminar. Next-Generation Sequencing Lunch & Learn
NGS: Digital RNAseq & Library Prep Seminar Next-Generation Sequencing Lunch & Learn Samuel Rulli, Ph. D Global Product Manager QIAseq Targeted RNA Panels 1 Targeted sequencing with UMIs QIAseq mirnaseq
More informationSMARTer Ultra Low RNA Kit for Illumina Sequencing Two powerful technologies combine to enable sequencing with ultra-low levels of RNA
SMARTer Ultra Low RNA Kit for Illumina Sequencing Two powerful technologies combine to enable sequencing with ultra-low levels of RNA The most sensitive cdna synthesis technology, combined with next-generation
More informationNGS-based innovations within the Leiden Network
NGS-based innovations within the Leiden Network A strong bridge between two partners Dr. Mark de Jong 2017-09-29 Design accurate and robust NGS tests and generate data sets essential for Diagnostics &
More informationThe Expanded Illumina Sequencing Portfolio New Sample Prep Solutions and Workflow
The Expanded Illumina Sequencing Portfolio New Sample Prep Solutions and Workflow Marcus Hausch, Ph.D. 2010 Illumina, Inc. All rights reserved. Illumina, illuminadx, Solexa, Making Sense Out of Life, Oligator,
More informationNextSeq 500 System WGS Solution
NextSeq 500 System WGS Solution An accessible, high-quality whole-genome sequencing solution for any species. Highlights High-Quality, High-Coverage Genome Illumina chemistry offers highest read quality
More informationDevelopment of quantitative targeted RNA-seq methodology for use in differential gene expression
Development of quantitative targeted RNA-seq methodology for use in differential gene expression Dr. Jens Winter, Market Development Group Biological Biological Research Content EMEA QIAGEN Universal Workflows
More informationIncreased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit
be INSPIRED drive DISCOVERY stay GENUINE TECHNICAL NOTE Increased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit Highly sensitive, robust generation of high quality
More informationOvercome limitations with RNA-Seq
Buyer s Guide Simple, customized RNA-Seq workflows Evaluating options for next-generation RNA sequencing Overcome limitations with RNA-Seq Next-generation sequencing (NGS) has revolutionized the study
More informationDetection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System
Detection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System Application Note Author Linus Forsmark Henrik Johansson Agilent Technologies Inc. Santa Clara, CA USA Abstract
More informationGENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS. Genomics Solutions Portfolio
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS Genomics Solutions Portfolio WORKFLOW SOLUTIONS FROM EXTRACTION TO ANALYSIS Application-based answers for every step of your workflow Scientists
More information02 Agenda Item 03 Agenda Item
01 Agenda Item 02 Agenda Item 03 Agenda Item SOLiD 3 System: Applications Overview April 12th, 2010 Jennifer Stover Field Application Specialist - SOLiD Applications Workflow for SOLiD Application Application
More informationAnalytics Behind Genomic Testing
A Quick Guide to the Analytics Behind Genomic Testing Elaine Gee, PhD Director, Bioinformatics ARUP Laboratories 1 Learning Objectives Catalogue various types of bioinformatics analyses that support clinical
More informationApplication Note Whole Transcriptome Sequencing from FFPE-derived RNA
pplication Note Whole Transcriptome Sequencing from FFPE-derived RN Sample uthors Nancy Nabilsi Senior pplications Scientist Drew Cheney pplications Scientist Jennifer Pavlica pplications Manager Rachel
More informationEpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina)
EpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiNext High-Sensitivity Bisulfite-Seq Kit is designed to prepare bisulfite-converted
More informationCM581A2: NEXT GENERATION SEQUENCING PLATFORMS AND LIBRARY GENERATION
CM581A2: NEXT GENERATION SEQUENCING PLATFORMS AND LIBRARY GENERATION Fall 2015 Instructors: Coordinator: Carol Wilusz, Associate Professor MIP, CMB Instructor: Dan Sloan, Assistant Professor, Biology,
More informationIntroduction to RNA-Seq in GeneSpring NGS Software
Introduction to RNA-Seq in GeneSpring NGS Software Dipa Roy Choudhury, Ph.D. Strand Scientific Intelligence and Agilent Technologies Learn more at www.genespring.com Introduction to RNA-Seq In a few years,
More informationresequencing storage SNP ncrna metagenomics private trio de novo exome ncrna RNA DNA bioinformatics RNA-seq comparative genomics
RNA Sequencing T TM variation genetics validation SNP ncrna metagenomics private trio de novo exome mendelian ChIP-seq RNA DNA bioinformatics custom target high-throughput resequencing storage ncrna comparative
More informationA Crash Course in NGS for GI Pathologists. Sandra O Toole
A Crash Course in NGS for GI Pathologists Sandra O Toole The Sanger Technique First generation sequencing Uses dideoxynucleotides (dideoxyadenine, dideoxyguanine, etc) These are molecules that resemble
More informationSingle-Cell Whole Transcriptome Profiling With the SOLiD. System
APPLICATION NOTE Single-Cell Whole Transcriptome Profiling Single-Cell Whole Transcriptome Profiling With the SOLiD System Introduction The ability to study the expression patterns of an individual cell
More informationMaximizing your NGS sequencing with IDT. Adam Chernick, PhD Field Applications Manager, Functional Genomics
Maximizing your NGS sequencing with IDT Adam Chernick, PhD Field Applications Manager, Functional Genomics 1 Contents Expanding our NGS portfolio what s next? xgen technology and Lockdown probe advantages
More informationSupplementary Information for:
Supplementary Information for: A streamlined and high-throughput targeting approach for human germline and cancer genomes using Oligonucleotide-Selective Sequencing Samuel Myllykangas 1, Jason D. Buenrostro
More informationPrepare libraries from degraded inputs with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for whole transcriptome sequencing
Sequencing Solutions Technical Note August 2018 How To Prepare libraries from degraded inputs with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for whole transcriptome sequencing Applications Whole
More informationExploring new frontiers with next-generation sequencing
Spotlight: NGS Exploring new frontiers with next-generation sequencing Pushing the limits of discovery Next-generation sequencing (NGS) is being utilized for numerous new and exciting applications, such
More informationReads to Discovery. Visualize Annotate Discover. Small DNA-Seq ChIP-Seq Methyl-Seq. MeDIP-Seq. RNA-Seq. RNA-Seq.
Reads to Discovery RNA-Seq Small DNA-Seq ChIP-Seq Methyl-Seq RNA-Seq MeDIP-Seq www.strand-ngs.com Analyze Visualize Annotate Discover Data Import Alignment Vendor Platforms: Illumina Ion Torrent Roche
More informationAPPLICATION NOTE. Abstract. Introduction
From minuscule amounts to magnificent results: reliable ChIP-seq data from 1, cells with the True MicroChIP and the MicroPlex Library Preparation kits Abstract Diagenode has developed groundbreaking solutions
More informationTargeted Sequencing in the NBS Laboratory
Targeted Sequencing in the NBS Laboratory Christopher Greene, PhD Newborn Screening and Molecular Biology Branch Division of Laboratory Sciences Gene Sequencing in Public Health Newborn Screening February
More informationGene Expression Technology
Gene Expression Technology Bing Zhang Department of Biomedical Informatics Vanderbilt University bing.zhang@vanderbilt.edu Gene expression Gene expression is the process by which information from a gene
More informationDigital DNA/RNA sequencing enables highly accurate and sensitive biomarker detection and quantification
Digital DNA/RNA sequencing enables highly accurate and sensitive biomarker detection and quantification Erwin Chen ( 陳立德 ) Technical Product Specialist QIAGEN Taiwan Precision medicine: Right drug, right
More informationSingle Cell Genomics
Single Cell Genomics Application Cost Platform/Protocol Note 3 mrna-seq Cell capture/rt/library prep $1,990/Sample 10x Genomics Chromium 500-10,000 cells/sample 5 V(D)J + mrna Cell capture/rt/library prep
More informationDNA. Clinical Trials. Research RNA. Custom. Reports CLIA CAP GCP. Tumor Genomic Profiling Services for Clinical Trials
Tumor Genomic Profiling Services for Clinical Trials Custom Reports DNA RNA Focused Gene Sets Clinical Trials Accuracy and Content Enhanced NGS Sequencing Extended Panel, Exomes, Transcriptomes Research
More informationA new paradigm in testing for NSCLC-targeted therapies
A new paradigm in testing for NSCLC-targeted therapies Accelerate results, from sample to report, with the first IVD NGS-based test NEW Oncomine Dx Target Test The Ion Torrent Oncomine Dx Target Test is
More informationILLUMINA SEQUENCING SYSTEMS
ILLUMINA SEQUENCING SYSTEMS PROVEN QUALITY. TRUSTED SOLUTIONS. Every day, researchers are using Illumina next-generation sequencing (NGS) systems to better understand human health and disease, as well
More informationHigh Throughput Sequencing the Multi-Tool of Life Sciences. Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center
High Throughput Sequencing the Multi-Tool of Life Sciences Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center DNA Technologies & Expression Analysis Cores HT Sequencing (Illumina
More informationTREE CODE PRODUCT BROCHURE
TREE CODE PRODUCT BROCHURE Single Molecule, Real-Time (SMRT) Sequencing technology offers: Long read sequencing ~10 Gb with 20 kb average read lengths for WGS ~20 Gb with 40 kb average read length for
More information