The response of research: from the field to the lab. K. Victoir PhD
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1 The response of research: from the field to the lab K. Victoir PhD
2 Outline Responses to the Ebola outbreak: a nations response (France) and an institutional response (IP) Overview of the Field actions in Guinea Highlight on some research actions in the context of the response
3 French Task Force on Ebola (Oct 2014) National Coordinator Pr Jean François Delfraissy, Under the authority of the Prime Minister Diplomacy Health Mrs Christine Fages, Pr Thierry Debord, Professor of Medicine Diplomat Interior/Security M. Pierre Lieutaud, Prefect Research Pr Yves Levy, CEO of Inserm, President of AVIESAN Administrative Chief Officer : Anne-Claire Amprou
4 Objectives of the French Task Force on Ebola Main tasks in October 2014 : - Avoid possible contamination in France - Support affected countries to curb the epidemic dynamics - Prevent the spread of the disease to neighboring countries - Coordination and funding of translational research on Ebola Coordinated response in Guinea : - Support political coordination in Guinea - HWs mobilization, training, and protection - Diagnosis and Treatment - Sensitization, education and prevention - Disease control measures
5 Objectives of the Institut Pasteur Ebola Task Force Support affected countries: Set of diagnostic laboratories Train local and international staff for a better preparedness and response Technical expertise Mobilise the Institut Pasteur International Network for contribution and mobilisation in home countries Perform research activities for short, medium and long term actions for advancements in: Novel diagnostics Better prevention (vaccines) Treatements
6 Work Packages Ebola Task Force Organizational Chart 1: Field Actions: Diagnostic labs, training Pasteur International Network Coordinators 2: Diagnostics: Genomic and Serology based tests 3: Vaccine Development: Adapt existing technologies Steering Committee -Wp leaders -Tech transfer -Project management -Ethic specialists -Communication -International Network -Legal department 4: Epidemiology & Genetic Studies: Molecular Virus evolution and Transmission Dynamics 5 : Novel Treatments: Antibody-based therapies, Cell-based screening of chemical compounds, Structure-based drug design 6 : Virus Pathogenesis
7 Treatment Centers in Guinea
8 Source: CDC
9 O. Faye, The Lancet/infection 2015
10 Better containment Early isolation/hospitalisation Diagnosis Safe burials Waste Management Contact tracing (long term) Information campaigns on transmission
11 Epidemiology and Genetic studies
12 Analysing Human and viral genetic sequences to improve diagnosis and management of Ebola epidemics space Whole Genome and Ebola virus GP gene Guinea VIRAL DYNAMICS IN TIME AND SPACE Dynamics of neutral and adaptive viral diversity across infection chains Characterization of transmission and social network Global spread and fate of variants
13 Treatements
14 Screening of molecules with broad spectrum antiviral activity 1 Chemical libraries chemical compounds Positive and negative controls Chemical screening facility platform IP Paris 2 Luminescence read-out functional screens 3 Hit identification hits appear to be potentially relevant for in vitro evaluation in the Ebola context (in parallel with chemical optimization, ADME-Tox tests and Mode of Action analysis, to be conducted also in Chikungunya and measles models)
15 Targeted drug design
16 NPC1(endosomal membrane protein) as a potential drug target Niemann-Pick C1 Cholesterol transporter Cote et al. Nature 2011 Understand the molecular mechanisms that trigger virus membrane fusion and the pharmacology associated to this process Structural characterization of the human NPC1 transporter (and homologs) and its complexes with viral proteins and small pharmaceutical compounds
17 Conclusion Continue training Continue technical support Continue preparedness actions Continue research but go up to application Promote integrative research No fatigue.
18 Merci Merci Thank you
19
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