Study Title Antimicrobial Activity and Efficacy of Seal Shield's Electroclave. Test Method Custom Device Study. Study Identification Number NG7233
|
|
- Dylan Harvey
- 6 years ago
- Views:
Transcription
1 Study Title Antimicrobial Activity and Efficacy of Seal Shield's Electroclave Test Method Custom Device Study Study Identification Number NG7233 Study Sponsor Christian Davis Seal Shield 3105 Riverside Avenue Jacksonville, FL (904) Test Facility Microchem Laboratory 1304 W. Industrial Blvd Round Rock, TX (512)
2 Test Device Information The test device, pictured below, was received on 25MAY2016. Test Microorganism Information The test microorganism(s) selected for this test: Electroclave Staphylococcus aureus (MRSA) This bacteria is a Gram-positive, cocci shaped, aerobe which is resistant to the penicillin-derivative antibiotic methicillin. MRSA can cause troublesome infections, and their rapid reproduction and resistance to antibiotics makes them more difficult to treat. MRSA bacteria are resistant to drying and can therefore survive on surfaces and fabrics for an extended period of time and therefore makes this bacteria an excellent representative for antimicrobial efficacy testing on surfaces. Enterobacter cloacae This bacteria is a Gram-negative, rod shaped, facultative anaerobe commonly found in the gastrointestinal tract of humans. It is not usually a primary pathogen although it is sometimes associated with urinary and respiratory tract infections. Although relatively susceptible to disinfection and desiccation when dried on a surface, it can be a challenging microorganism to mitigate in solution. Page 2 of 8
3 Summary of the Procedure Test microorganisms are prepared in TSB and incubated overnight. The test culture is diluted as necessary in PBS targeting a concentration 1x10 6 CFU/carrier. Sterile glass slides (test carriers) are inoculated with ml of test culture over approximately 2 square inches and incubated at 36 ± 1 C for approximately 20 minutes, or until visually dry. Test carriers are treated according to device manufacturer's instruction. Treated and control test carriers are harvested in 20 ml of neutralizing broth or PBS and enumerated using standard dilution and plating techniques. Microbial concentrations are determined at time zero by analysis of control materials immediately after inoculation with microorganisms. Additional microbial concentrations are determined at each contact time to account for potential desiccation due to drying. All plates are incubated at 36 ± 1 C for approximately hours. After incubation, microbial concentrations are determined and the reduction of microorganisms is calculated relative to the control materials. Study Timeline Culture Initiated Culture Harvested Carrier Inoculated Carrier Harvested Carriers Evaluated Report Delivered 26MAY MAY MAY MAY MAY JUN JUN JUN JUN JUN JUN2016 Note: Dates in row 1 above detail progression of MRSA testing. Row 2 details progression of CRE testing. Page 3 of 8
4 Criteria for Scientific Defensibility of a Custom Device Study For Microchem Laboratory to consider a Device Study study to be scientifically defensible, the following criteria must be met: 1. The average number of viable bacteria recovered from the time zero samples must be approximately 1 x 10 6 cells/carrier or greater. 2. Positive/Growth controls must demonstrate growth of the appropriate test microorganism. 3. Negative/Purity controls must demonstrate no growth of test microorganism. Passing Criteria Because of the nature of the study, passing criteria may be determined by the Study Sponsor. Testing Parameters used in this Study Test Device Mode of Use: UV Light Carrier: Sterile 1 x 3 Glass Slides Replicates: 3 (test), 1 (control) Culture Growth Media: TSB Culture Growth Time: 24 ± 4 hours Culture Dilution Media: PBS, if applicable Culture Supplement: None Inoculum Concentration: 1.0 x 10 6 CFU/Carrier Inoculum Volume: 0.020ml/2in 2 : 1 contact times, see notes Contact Temperature: Ambient Neutralizer (Vol.): D/E Broth (20 ml) Enumeration Media: TSA Enumeration Plate Incubation Temperature: Enumeration Plate 36 C ± 1 C Incubation Time: hours Page 4 of 8
5 Study Notes Antimicrobial efficacy was evaluated at 3 contact times: 13 minutes and 33 seconds Overnight test cultures were not modified (other than dilution in PBS for S. aureus) prior to testing. Study Photographs Left Image: Inoculated carrier placement prior to treatment. Right Image: Bays 2 and 3 during treatment. Page 5 of 8
6 Control Results Neutralization Method: N/A Growth Confirmation: Confirmed Media Sterility: Confirmed Calculations Where: B = Number of viable test microorganisms on the control carriers A = Number of viable test microorganisms on the test carriers after the contact time Where: B = Number of viable test microorganisms on the control carriers A = Number of viable test microorganisms on the test carriers after the contact time Page 6 of 8
7 Results of the Study Table 2. S. aureus (MRSA) results at 13 minutes and 33 seconds Microorganism Test Device (minutes:seconds) Carrier Identification Replicate CFU/Carrier Average CFU/Carrier Percent Reduction vs. Control at Log 10 Reduction vs. Control at S. aureus ATCC MRSA Electroclave Control at Time Zero 5.80E E minutes 33 seconds Control 9.40E E+06 Left Middle 2.10E E+03 N/A 1.94E % 3.69 Right 1.88E E E E+06 Replicate CFU/Carrier 1.00E E E E E E+00 Control at Control at Replicate 1 Replicate 2 Replicate 3 Time Zero 13 minute and 33 second contact time Page 7 of 8
8 Results of the Study Table 5. E. cloacae (CRE) results at 13 minutes and 33 seconds Microorganism Test Device (minutes:seconds) Carrier Identification Replicate CFU/Carrier Average CFU/Carrier Percent Reduction vs. Control at Log 10 Reduction vs. Control at E. cloacae ATCC BAA-2468 CRE Electroclave Control at Time Zero 1.20E E minutes 33 seconds Control 6.30E E+06 Left Middle 1.69E E+02 N/A 1.44E % 3.64 Right 1.71E E E E+06 Replicate CFU/Carrier 1.00E E E E E E+00 Control at Control at Replicate 1 Replicate 2 Replicate 3 Time Zero 13 minute and 33 second contact time Page 8 of 8
Study Title Antibacterial Efficacy of Bio-Care Technology's Non-Porous Test Substance
Study Title Antibacterial Efficacy of Bio-Care Technology's Non-Porous Test Substance Test Method Japanese Industrial Standard Z 2801 Antibacterial Products Test for Antibacterial Activity and Efficacy
More informationEfficacy Report Summarization for SoClean 2
Efficacy Report Summarization for SoClean 2 October 2017 SoClean Inc 36 Town Forest Road Oxford, Massachusetts 01540 USA Tel. 508-363-0418 Email info@soclean.com SoClean 2 is USA FDA Registered 3009534409
More informationThis study report is published with the express written consent of Antimicrobial Test Laboratories. Business Mobility Now!
mobile Antimicrobial Lab Test Report The Socket SoMo 650Rx handheld computer incorporates antimicrobial materials that provide an extra layer of protection to the device against the spread and multiplication
More informationTest Method for Efficacy of Copper Alloy Surfaces as a Sanitizer
Test Method for Efficacy of Copper Alloy Surfaces as a Sanitizer Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa (ATCC 15442) Methicillin Resistant
More informationASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA
Final Report submitted to Virox Technologies Inc. Oakville, Ontario ASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA Syed A. Sattar,
More informationFINAL REPORT Efficacy of a Cold Plasma System
FINAL REPORT Efficacy of a Cold Plasma System ORDER Number PREPARED FOR: 714 Mall Blvd., Suite 3 Savannah, GA 31406 EMSL Analytical, Inc. Web: http://www.emsl.com - 1 - Certificate of Analysis Client:
More informationMethod Suitability Report Membrane Filtration Sterility Test with QTMicro Apparatus
Method Suitability Report Membrane Filtration Sterility Test with QTMicro Apparatus Bevacizumab (Avastin) 1.25 mg/0.05ml This report provides details on the specifics of a Membrane Filtration Sterility
More informationPRESERVATIVE EFFICACY TEST FOR COSMETIC PRODUCT
1 SCOPE AND FIELD OF APPLICATION To determine the efficacy of the antimicrobial activity of preservatives used in cosmetic products. The method covers the determination of the suitability of preservation
More informationTest Organisms: Staphylococcus aureus (ATCC 6538) Escherichia coli (ATCC 11229) Pseudomonas aeruginosa (15442)
STUDY TITLE Evaluation of Antimicrobial Activity of UV Illumination/Hydroxyl Generator s: (15442) PRODUCT IDENTITY Odorox Mobile Disinfection Unit Hydroxyl Generator AUTHOR Scott R. Steinagel, B.S. Manager,
More informationThe Microbiological Requirements of a Stability Study. Ngoc Anh-Thu Phan 19 th June 2012
The Microbiological Requirements of a Stability Study Ngoc Anh-Thu Phan 19 th June 2012 Contents Introduction Harmonized Tests Acceptance Criteria Selection of Tests Why PE Testing? Selection of Category
More informationBiofilm Protocol Optimization For Pseudomonas aeruginosa. Introduction. Materials and Methods. Culture Media, Incubation Time, and Biofilm Measurement
Biofilm Protocol Optimization For Pseudomonas aeruginosa Culture Media, Incubation Time, and Biofilm Measurement Introduction In addition to the conventional arsenal of antibiotic resistance mechanisms
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationThe AOAC Germicidal Spray Products as Disinfectants
DISINFECTANTS Pines et al.: Journal of AOAC International Vol. 96, No. 3, 2013 567 Procedural Revision to the AOAC Germicidal Spray Products as Disinfectants Test Method: Establishment of Minimum and Maximum
More informationFINAL REPORT STUDY TITLE
Final Report: Germicidal Spray Test of ZeroMold Plus Page 1 of 18 FINAL REPORT STUDY TITLE Germicidal Spray Test of ZeroMold Plus Disinfectant SOUTHWEST RESEARCH INSTITUTE PROJECT NUMBERS 01.19898.01.001
More informationComparison of the speed of kill of pathogenic bacteria using ACTICOAT and AQUACEL Ag analysed using Confocal Laser Scanning Microscopy
Smith & Nephew Wound Management Data on file report - 0505004 May 2005 Comparison of the speed of kill of pathogenic bacteria using ACTICOAT and AQUACEL Ag analysed using Confocal Laser Scanning Microscopy
More informationABC. Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Volume 19 Number 18
M26-A ISBN 1-56238-384-1 September 1999 ISSN 0273-3099 Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline Volume 19 Number 18 Arthur L. Barry, Ph.D. William A. Craig,
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationAntibiofilm Efficacy of Advanced Formula Zymox Otic Plus with 1% Hydrocortisone
Antibiofilm Efficacy of Advanced Formula Zymox Otic lus with 1% Hydrocortisone Rajvinder Atwal, hd¹ Anim Omar, h.d., B. harma² Merle Oson, DVM, MSc.² Nicole Butler, B.Sc.² Yanira Caberra, B.Sc.² ¹Laclede
More informationGuidelines for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel
Guidelines for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel Purpose The Clinical Laboratory Improvement Amendments (CLIA), passed in 1988, establishes
More informationMICROBIAL GROWTH. Dr. Hala Al-Daghistani
MICROBIAL GROWTH Dr. Hala Al-Daghistani Microbial Growth Microbial growth: Increase in cell number, not cell size! Physical Requirements for Growth: Temperature Minimum growth temperature Optimum growth
More informationIndigo-Clean White Paper: #1 Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species. Healthcare
Indigo-Clean White Paper: #1 Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species Healthcare HC Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species Clifford J. Yahnke,
More informationSpecifications, Methods and precedence of source with particular reference to microbiological requirements in TGOs.
Specifications, Methods and precedence of source with particular reference to microbiological requirements in TGOs. Fergus O Connell RACI Pharmaceutical Science Group (NSW) 10/08/2015 Currently there are
More informationTests to Support Sterility Claim. Imtiaz Ahmed
Tests to Support Sterility Claim Imtiaz Ahmed Sterile Product As per TGO 77, a sterile product must comply with the requirements of the following tests: Sterility Test Bacterial Endotoxins Test Appendix
More informationGLP TESTING OF ZEROMOLD PLUS FOR ACINETOBACTER BAUMANNII
Southwest Research Institute Page 1 of 10 Microencapsulation and Nanomaterials Department Final Report: GLP Testing of ZeroMold Plus for Acinetobacter baumannii Protocol No.: GLP-SP-242 FINAL REPORT STUDY
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationImmobilization and Death of Bacteria by Flora Seal Microbial Sealant
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 6 Issue 6 June 2017 PP. 45-49 Immobilization and Death of Bacteria by Flora Seal Microbial
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More information6/28/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics
Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationá61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS
USP 40 Microbiological Tests / á61ñ Microbiological Examination 1 á61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS INTRODUCTION The tests described hereafter will allow
More information10/2/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics
Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical
More informationINSTRUCTIONS FOR USE LYFO DISK KWIK-STIK KWIK-STIK PLUS. n LYFO DISK Microorganisms n KWIK-STIK Microorganisms n KWIK-STIK Plus Microorganisms
INSTRUCTIONS FOR USE n LYFO DISK Microorganisms n KWIK-STIK Microorganisms n KWIK-STIK Plus Microorganisms LYFO DISK INTENDED USE LYFO DISK, KWIK-STIK and KWIK-STIK Plus Microorganisms are lyophilized,
More informationAn Overview of Antimicrobial Testing for Textile Applications
An Overview of Antimicrobial Testing for Textile Applications By H. Wayne Swofford, Microban International Introduction Antimicrobial treatment of textiles has three key benefits: Odor control, providing
More informationIsolation & Characterization of Bacteria
PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!
More informationMicrobial Separation from a Complex Matrix by a Hand-Held Microfluidic Device
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Supplementary Information Microbial Separation from a Complex Matrix by a Hand-Held Microfluidic
More informationá62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS
USP 40 Microbiological Tests / á62ñ Microbiological Examination 1 á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS INTRODUCTION The tests described hereafter
More informationAutomated methods in the Microbiology Lab-Issues and Troubleshooting! Presented By: Dr B. Oboudi
In the name of GOD Automated methods in the Microbiology Lab-Issues and Troubleshooting! Presented By: Dr B. Oboudi Overview Background/history Plate streakers and Gram stainers Blood cultures Automated
More informationProject 5: Urine Cultures and Identification
Project 5: Urine Cultures and Identification Readings: http://www.webmd.com/a-to-z-guides/urine-culture http://www.medscape.com/viewarticle/558845 (Listen to the two lectures by Dr. Robert A. Weinstein.)
More informationINTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants
INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly
More informationMicrobiological Quality Control as Described in the Compendia. Scott Sutton, Ph.D.
Microbiological Quality Control as Described in the Compendia Scott Sutton, Ph.D. Microbiological Quality Control as Described in the Compendia Antimicrobial Effectiveness Test , ,
More informationBACTERIAL CONJUGATION. To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another.
BACTERIAL CONJUGATION I. OBJECTIVES To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another. To learn about the various genetic elements
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationwhy your next CO 2 incubator should be 100% pure copper
Thermo Scientific 100% Pure Copper CO 2 Incubators 5 reasons why your next CO 2 incubator should be 100% pure copper We like the Heracell copper incubator because we just don t experience any problems,
More information2.1 Tryptone Soya Broth containing 4% Tween 80 (TSB + T), or another appropriate deactivating broth.
Prepared by: Date: Supersedes: Checked by: Date: Date Issued: Approved by: Date: Review Date: 1. SCOPE AND APPLICATION This general test method applies to the determination of microbiological test method
More informationDEPARTMENT OF CLINICAL LABORATORY SCIENCES SCHOOL OF HEALTH TECHNOLOGY AND MANAGEMENT THE UNIVERSITY AT STONY BROOK STONY BROOK, NEW YORK
DEPARTMENT OF CLINICAL LABORATORY SCIENCES SCHOOL OF HEALTH TECHNOLOGY AND MANAGEMENT THE UNIVERSITY AT STONY BROOK STONY BROOK, NEW YORK 11794-8205 MICROBIOLOGY COMPETENCY EVALUATION FORM STUDENT NAME:
More informationEM021. Co-Trimoxazole Ezy MIC TM Strip (COT)( mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing
Co-Trimoxazole Ezy MIC TM Strip (COT)(0.002-32 mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM021 Not for Medicinal Use It is a unique MIC
More informationVerification of Gradient Diffusion Strips
Verification of Gradient Diffusion Strips Objectives 1. Describe gradient diffusion tests 2. Describe process of FDA clearance of susceptibility tests 3. Discuss CLIA requirements for laboratory verification
More informationDISINFECTION QUALIFI CATION TESTING CONSIDERATIONS FOR THE ASEPTIC AND CLEANROOM MANUFACTURING ENVIRONMENT
DISINFECTION QUALIFI CATION TESTING CONSIDERATIONS FOR THE ASEPTIC AND CLEANROOM MANUFACTURING ENVIRONMENT by Dave Rottjakob, M.T. (ASCP) December, 2013 INTRODUCTION Obtaining the highest confidence that
More informationMICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods
Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most
More informationLab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab
Lab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab OBJECTIVES 1. Understand the use of MPN to determine likely fecal water contamination. 2. Understand the use of MUG,
More informationVirginia Western Community College BIO 205 General Microbiology
Prerequisites BIO 205 General Microbiology One year of college biology and one year of college chemistry or divisional approval; an ENG 111 placement recommendation, co-enrollment in ENF 3/ENG 111, or
More informationActivity 5.1.4: Gram Staining
Activity 5.1.4: Gram Staining Introduction In the last activity, you isolated the bacteria that was responsible for Anna s illness and performed a gross examination of the resultant colonies. While gross
More informationFinal text for addition to The International Pharmacopoeia
March 2012 3.3.2 MICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS Final text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth
More informationAdapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692
Biology 4B Laboratory Bacteriological Examination of Water Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Objectives Carry out
More informationFinal text for addition to The International Pharmacopoeia
March 2012 3.3.1 MICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: MICROBIAL ENUMERATION TESTS Final text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth
More informationInt.J.Curr.Microbiol.App.Sci (2014) 3(10)
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 810-815 http://www.ijcmas.com Original Research Article Comparison of Tissue Culture plate method, Tube Method and Congo Red Agar Method for the detection
More informationInactivation of Emerging Pathogens and Continuous Room Decontamination Technologies
Inactivation of Emerging Pathogens and Continuous Room Decontamination Technologies William A. Rutala, Ph.D., M.P.H. Director, Statewide Program for Infection Control and Epidemiology, Research Professor
More informationFinally, You Have a Choice! Online Ordering Available
Simple and Effective System for Colony Counting Finally, You Have a Choice! Online Ordering Available Compact Dry is a ready-touse system reducing the time needed to perform microbial testing on food,
More informationTRYPTIC SOY AGAR (TSA) WITH LECITHIN AND TWEEN 80
TRYPTIC SOY AGAR (TSA) WITH LECITHIN AND TWEEN 80 Cat. no. P45 TSA with Lecithin and Tween 80, 15x60mm Contact Plate, 15ml Cat. no. Q13 TSA with Lecithin and Tween 80, 20x125mm Tube, 18ml Deep Cat. no.
More informationRapid Aerobic Count. Interpretation Guide. 3M Food Safety 3M Petrifilm Rapid Aerobic Count Plate
3M Food Safety 3M Petrifilm Rapid Aerobic Count Plate Rapid Aerobic Count Interpretation Guide The 3M Petrifilm Rapid Aerobic Count Plate is a sample-ready culture medium system which contains nutrients,
More informationCompounding Pharmacies and the USP <71> Sterility Tests
Compounding Pharmacies and the USP Sterility Tests Scott Sutton, Ph.D. scott.sutton@microbiol.org 49 41 Disclaimer I am making this presentation as an independent agent I am not making this presentation
More informationBD GeneOhm MRSA ACP Lysis Kit
REF 441638 100 Tests DOPS09-09-V1E1 USA Date: 2010-01 TABLE OF CONTENTS INTENDED USE...3 SUMMARY AND EXPLANATION OF THE PROCEDURE...3 REAGENTS...3 PRECAUTIONS...3 MATERIALS PROVIDED...4 STORAGE, HANDLING
More informationLab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic
Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative
More informationBBL QualiSwab Quality Control Culture Devices
Revisions Rev from Rev to ECO # 0299 0704 2996-04 SO 0191-5 Notes: 1. BD Cat. Number Various 2. Blank (Sheet) Size : Length: 11 Width: 8.5 Number of Pages: 4 Number of Sheets: 1 Page Size: Length 8.5 Width
More information3 Petrifilm Environmental Listeria Plate for the Rapid Enumeration of Listeria from Environmental Surfaces
3 Petrifilm Environmental Listeria Plate for the Rapid Enumeration of Listeria from Environmental Surfaces Barbara Horter and Henry Lubrant, 3M Microbiology, St. Paul, MN, USA Abstract Accurate and rapid
More informationEFFECTIVITY DETERMINATION OF THE PRODUCT AQUAFINESSE HOT TUB WATERTREATMENT
KEMA TEST KEMA Nederland B.V. Utrechtseweg 310, 6812 AR Arnhem Postbus 9035, 6800 ET Arnhem T (026) 3 56 91 11 F (026) 3 89 24 77 contact@kema.com www.kema.com Handelsregister Arnhem 09080262 APPENDIX
More informationPaper Reference. Paper Reference(s) 1520/4H Edexcel GCSE Biology A [1520] Paper 4H Higher Tier Wednesday 21 June 2006 Morning Time: 1 hour
Centre No. Candidate No. Paper Reference(s) 1520/4H Edexcel GCSE Biology A [1520] Paper 4H Higher Tier Wednesday 21 June 2006 Morning Time: 1 hour Materials required for examination Nil Paper Reference
More informationVerification of Disk Diffusion Tests
Verification of Disk Diffusion Tests Objectives 1. Describe disk diffusion tests 2. Describe process of FDA clearance of susceptibility tests 3. Discuss CLIA requirements for laboratory verification of
More informationKILL-TIME STUDIES Antimicrobial Activity of EO H 2 O Using Clostridium difficile spores Test Solutions: Acidic H 2 O Submitted December 12, 2011
KILL-TIME STUDIES Antimicrobial Activity of EO H 2 O Using Clostridium difficile spores Test Solutions: Acidic H 2 O Submitted December 12, 2011 December 20, 2011 PREPARED FOR: Rob Dahl Viritec, LLC. BY:
More informationSelection of Storage Methods for Maintenance of Different Stock Cultures
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 10 (2016) pp. 1097-1104 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.510.115
More informationEzy MIC Strip FEATURES AND ADVANTAGES
Imipenem with & without EDTA Ezy MIC Strips (IPM+EDTA/IPM) (Imipenem + EDTA: 1-64 mcg/ml) (Imipenem : 4-256 mcg/ml) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM078 Not for Medicinal
More informationTable of Contents. A Culture of Service
102714tr Table of Contents 1 BlueEcoli 2 Candida 3 CRE 4 ECC 5 HurBi 6 ESBL 7 Listeria 8 MRSA 9 O157 10 Sakazakii 11 Salmonella 12 SS 13 Staph aureus 14 UTI 15 Vibrio Headquarters A Culture of Service
More informationSTERILIZATION VALIDATION IN THIS SECTION REPROCESSING VALIDATIONS FOR REUSABLE MEDICAL DEVICES. Radiation Sterilization Validation
STERILIZATION VALIDATION All sterilization processes require validation of the efficacy and reproducibility of the process. Depending on the type of sterilization, this may be accomplished by partial,
More informationProtocol Reference: Verification Protocol
Protocol Reference: Verification Protocol Contents 1. About BioBall Multishot 550 3 2. About This Protocol 4 3. Safety Precautions 4 4. Protocol Pre-approval 4 5. Protocol Scope 5 5.1. Intended Location
More informationHow antimicrobial agents work
Physical and Chemical Control of Microbes Physical Agents heat or radiation Chemical Agents disinfectants or antiseptics Important Terms 1. Sterilization process of killing all viable microbes 2. Bactericide
More informationHARDWARE MADE WITH BACTERICIDAL COPPER
HARDWARE MADE WITH BACTERICIDAL COPPER DID YOU KW? The door handle of a public facility can be touched several thousand times per day. That is thousands of opportunities to transfer bacteria and infectious
More informationChromogenic Culture Media
Color is my day-long obsession, joy, and torment. - Claude Monet Chromogenic Culture Media A Culture of Service Contents BluEcoli CRE Candida ECC E. coli O157 ESBL HUrBi Listeria MRSA SS (Salmonella Shigella)
More informationRAC. Interpretation Guide. Rapid Aerobic Count Plate
Interpretation Guide The 3M Petrifilm is a sample-ready-culture medium system which contains nutrients, a cold-water-soluble gelling agent and a dual-sensing indicator technology that facilitates aerobic
More informationChapter 6: Microbial Growth
Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends
More informationChapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method)
Chapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method) The disk diffusion method presented in this chapter has been carefully standardized by the National Committee for Clinical Laboratory
More informationISAH Warsaw, Poland Vol 2
COMPARISON OF THE SPREAD PLATE TECHNIQUE AND THE MPN-TECHNIQUE FOR THE DETERMINATION OF VIABLE COLONY COUNTS IN QUANTITATIVE SUSPENSION TESTS TO EVALUATE BACTERICIDAL AND FUNGICIDAL ACTIVITY OF CHEMICAL
More informationSANIDATE 5.0 -BLUEBERRY STUDY
22 Meadow Street East Hartford, CT 06108 Phone: 860.290.8890 Fax: 860.290.8802 Solutions@BioSafeSystems.com 1-877-358-1299 www.biosafesystems.com www.enviroselects.com SANIDATE 5.0 -BLUEBERRY STUDY PI:
More informationDID YOU KNOW? Acquired Infections (HAI s) reported every year!
A N T I M I C R O B I A L CO P P E R H A R DWA R E Trimco Healthy Hardware DID YOU KW? The door handle of a public facility can be touched several thousand times per day. That is thousands of opportunities
More informationBIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014)
BIOLOGY 163 LABORATORY THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) Bacteria are single-celled prokaryotic organisms. As bacterial cells take in nutrients from their
More informationIntroduction, by Tortora, Funke and Case, 11th Ed. TENTATIVE LECTURE OUTLINE DATE TOPIC CHAPTER
MICROBIOLOGY 220 Spring 2014 TTh Section Professor: Scott Rose Text: Microbiology; An Introduction, by Tortora, Funke and Case, 11th Ed. TENTATIVE LECTURE OUTLINE DATE TOPIC CHAPTER Jan. 23 Introduction
More informationSwab type, moistening, and pre-enrichment for Staphylococcus aureus on environmental. Timothy F. Landers* 1. Armando Hoet 2. Thomas E.
JCM Accepts, published online ahead of print on April 00 J. Clin. Microbiol. doi:0./jcm.0-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
More informationMicrobial Growth and The Control of Microbial Growth (Chapter 6 & 7)
Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus Primary Source for figures and content:
More informationDisinfectant Qualification A Multifaceted Study
Disinfectant Qualification A Multifaceted Study In this article, Ziva Abraham, Microrite, Inc., gives us a brief overview of the disinfectant qualification study process. An effective cleaning and disinfection
More informationGENUS STAPHYLOCOCCUS: Isolation and Identification
GENUS STAPHYLOCOCCUS: Isolation and Identification Staphylococcus is a genus of Gram +, nonspore-forming cocci belonging to the family Micrococcaceae that are often found as normal human microbiota of
More informationStudy Summary. Results: All tested media-filled vials were negative for growth of any microorganisms.
A 7 Days Microbial Ingress Test of Single Use Vials Utilizing the EQUASHIELD Closed System Drug Transfer Device Study by Nelson Laboratories (Salt Lake City, UT) 2013, updated in 2014* Study Summary Abstract:
More informationManaging Your Environmental Isolates
Managing Your Environmental Isolates Good Testing Practices Good practice includes the periodic challenge of media with low levels of organisms. This includes USP indicator organisms as well as normal
More informationBacteriological Analytical Manual Online January 2001
Page 1 of 5 FDA Home Page CFSAN Home Search/Subject Index Q & A Help Bacteriological Analytical Manual Online January 2001 Chapter 12 Staphylococcus aureus Authors (Return to Table of Contents) Staphylococcus
More informationAntimicrobial Drugs. Antimicrobial Drugs. The dawn of antibiotics. Alexander Fleming. Chain and Florey. Antibiotics
Antimicrobial Drugs Antimicrobial Drugs Chemotherapy: The use of drugs to treat a disease Antimicrobial drugs: Interfere with the growth of microbes within a host Antibiotic: Substance produced by a microbe
More informationMedia Fill Test Kits USP <797> COMPLIANT. HARDY DIAGNOSTICS. Low Risk. Medium Risk. High Risk
HARDY DIAGNOSTICS Media Fill Test Kits USP COMPLIANT Low Risk Medium Risk High Risk Validate the proficiency of your technicians and pharmacists per USP Test for sterility per USP www.hardyvalkits.com
More informationTransformation of Bacillus subtilis with prit4501 and prit4502
Transformation of Bacillus subtilis with prit4501 and prit4502 Plasmids prit4501 and prit4502 were created by fusing the E. coli plasmid puc9 with the B. subtilis plasmid pbac. The recombinants therefore
More information55 Industrial Park Road Boothbay, ME USA
Introduction Increasing the level of sanitation and sterility of health-care environments is critical in reducing the transmission of health-care associated infections. The Center for Disease Control estimates
More informationELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA) Varandt Y. Khodaverdian 1* and Menachem Shoham 2
ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA) Varandt Y. Khodaverdian 1* and Menachem Shoham 2 1 Department of Biology, Tufts University, Medford, USA; 2 Department
More informationMIC and MBC Evaluation of Pure Products Antimicrobial Product against Selected Bacteria:
01/28/2016 16:40 FAX 8013925192 MOTHER EARTH MINERALS 14)001 1/28/16 To: Jim Dooley From; Mother Earth Minerals (Five Pages) MIC and MBC Evaluation of Pure Products Antimicrobial Product against Selected
More informationMethicillin resistant Staphylococcus aureus (MRSA)- Background and analysis
Methicillin resistant Staphylococcus aureus ()- Background and analysis Outline Staphylococcus aureus Classification Ecology Commensal vs pathogenic S. aureus Definition Resistance determinant and mechanism
More information