DURACLONE IM ACCELERATE YOUR PACE IN IMMUNE SYSTEM RESEARCH. For Reseach Use Only - Not for use in Diagnostic procedures
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1 DURACLONE IM ACCELERATE YOUR PACE IN IMMUNE SYSTEM RESEARCH Your clinical research trial companion For Reseach Use Only - Not for use in Diagnostic procedures
2 ACCELERATE YOUR PACE IN IMMUNE SYSTEM RESEARCH Antibody panel development requires a high level of flow cytometry expertise and may consume critical laboratory resources. Take a leap forward and streamline panel implementation in your laboratory by using expert proven, dry DuraClone IM antibody combinations - the ideal solution for longitudinal and mult-center clinical research trials! DEVELOPMENT OF ANTIBODY PANELS FOR FLOW CYTOMETRY in-house, using liquid single color antibodies Select antibody clones Select dye combination Performance OK Verify panel performance Optimize preparation protocol Create SOP for cocktail mixing & QC Transfer assay into lab Performance failure, revisit design process using DuraClone Pre-formulated DuraClone IM panels eliminate development workload Transfer assay into lab Advancing Science Through Discovery
3 DuraClone IM PANELS Antibody combinations for immune system research The dry DuraClone IM antibody panels are optimized for the identification and characterization of cells of the human immune system. Pre-formulated antibody panels up to 10 colors Unitized dry Beckman Coulter proprietary format Expert-proven marker combinations 25 tests/package including 3 compensation kits Designed by expert labs from 405 nm 488 nm 633 nm Product PB KrO FITC PE ECD PC5.5 PC7 APC AF647 AF700 IM Phenotyping Basic Tube B53309 (25 tests RUO) IM B Cell Tube B53318 (25 tests RUO) IM T Cell Subsets Tube B53328 (25 tests RUO) IM Dendritic Cells Tube B53351 (25 tests RUO) IM TCRs Tube B53340 (25 tests RUO) IM Treg Tube B53346 (25 tests RUO) IM Granulocytes Tube B88651 (25 tests RUO) IM Count Tube C00162 (25 tests RUO) AF700 - CD45 CD16 CD56 CD19 - CD14 CD4 - CD8 - CD3 IgM CD45 IgD CD21 CD19 - CD27 CD CD38 CD57 CD45 CD45RA CCR7 CD28 PD1 CD27 CD4 - CD8 - CD3 HLA-DR CD45 CD16 Lineage ** - CD1c CD11c Clec9A - - CD123 - TCRVd2 CD45 TCRgd TCRab HLA-DR - TCRVd1 CD4 - CD8 - CD3 Helios CD45 CD45RA CD25 - CD39 CD4 - FoxP3 - - CD3 CD15 CD45 CD294 - CD16 CD33 CD11b PD-L1 - - Lineage *** CD62L - - CD45 Counting Beads 7-AAD ISO ** CD3 / CD19 / CD20 / CD14 / CD56 *** CD3 / CD14 / CD19 / CD56 PB: Pacific Blue* KrO: Krome Orange AF647: Alexa Fluor* 647 AF700: Alexa Fluor* 700 : Alexa Fluor* 750 AF700: Alexa Fluor* 700 : Alexa Fluor* 750 * Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.
4 MINIMIZE YOUR REAGENT INVENTORY MANAGEMENT Working with cocktails prepared from liquid antibodies is associated with complex and time consuming inventory management impacting laboratory productivity. DuraClone IM kits simplify inventory management dramatically by replacing multiple different single color reagents with just 1 pre-formulated product. Reagent Inventory Management Liquid Cocktails from Single Color Reagents DuraClone IM QC of reagent lots Format Reagent waste sources Multiple single color reagent lots for each panel Liquid Mix from single color reagents with varying test volumes and staggered expiry dates Pipetting failure Unused remnant volume Non-stochiometric consumption of single color reagents 1 reagent lot for each panel Dry Unitized per test None Storage / Shipping conditions Refrigerated Room temperature Advancing Science Through Discovery
5 STREAMLINE YOUR WORKFLOW WITH DuraClone IM In the DuraClone IM kits every assay tube contains a layer with the dried-down antibody panel. The elimination of antibody pipetting shortens the turn-aroundtime, minimizes human error sources and purges the workflow from repetitive actions that are expensive to automate. Workflow for DuraClone IM Panels* Add whole blood sample & incubate Lysis Wash Acquire * DuraClone IM Treg uses a modified protocol for intracellular staining, please see the instructions for use for details. Assay tubes and compensation kits contain identical tandem dye-labeled antibodies to assure accurate and easy compensation.
6 DATA EXAMPLES FROM THE DuraClone IM TOOLBOX IM Treg CD4-PC7 CD25-PE Helios PB Identification and characterization of FoxP3+ regulatory T cells using a fast permeabilization protocol. CD3-A750 FoxP3-A647 CD39-PC5.5 IM Treg Tube PB KrO FITC PE ECD PC5.5 PC7 APC AF647 B53346 (25 tests RUO) Helios CD45 CD45RA CD25 - CD39 CD4 - FoxP3 CD3 ISO IM Granulocytes CD294-FITC SS INT CD15-PB Classifies major granulocyte subpopulations including assessment of maturation- or activation-dependent CD11b, CD16, CD62L and PD-L1 (CD274) co-expression. LIN AF700 CD15-PB LIN AF700 IM Granulocytes Tube PB KrO FITC PE ECD PC5.5 PC7 APC AF700 B88651 (25 tests RUO) CD15 CD45 CD294 - CD16 CD33 CD11b PD-L1 Lineage *** CD62L ISO *** CD3 / CD14 / CD19 / CD56 IM Count FL2 INT SS INT SS INT Absolute counting/viability for whole blood samples and versatile counting reference for all DuraClone IM panels. CD45-FITC CD45-FITC 7-AAD IM Count Tube PB KrO FITC PE ECD PC5.5 PC7 APC AF700 C00162 (25 tests RUO) - - CD45 Counting beads - 7-AAD ISO Advancing Science Through Discovery
7 MORE DuraClone PANELS DuraClone IF For Immune Function The DuraClone IF antibody panels reveal immune function at the single cell level utilizing a streamlined and simplified intracellular staining workflow. 405 nm 488 nm 633 nm Product PB KrO FITC PE ECD PC5.5 PC7 APC AF700 IF T Activation B88649 (25 tests RUO) IF T Helper Cell (2017, product configuration not final) CD4 - IFNg TNFa - - IL-2 - CD8 CD3 IL-17 - IFNg IL-4 CD4 - CD3 ISO DuraClone RE For Rare Event Detection The DuraClone RE panels enable sensitive detection of rare abnormal cell populations in blood or bone marrow using carefully optimized and highly dosed antibody combinations. 405 nm 488 nm 633 nm Product PB KrO FITC PE ECD PC5.5 PC7 APC RE CLB Tube B80393 (25 tests RUO) RE PC Tube B80394 (25 tests RUO) RE ALB Tube C00163 (25 tests RUO) AF700 CD20 CD45 CD81 ROR-1 - CD79b CD19 CD5 - CD43 CD38 CD45 CD81 CD27 - CD19 CD200 CD138 - CD56 - CD45 CD58 - CD34 CD10 CD19 - CD38 CD20 standard ISO
8 Choose Beckman Coulter for Benchmark Expertise and Innovation For over 80 years Beckman Coulter has driven innovation. We remain committed to shaping flow cytometry technology to fit seamlessly into your lab s workflow and to provide an optimal user experience. When you choose a Beckman Coulter solution you receive the high level of expertise, innovation, and quality assurance. Contact your sales representative or visit Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter s worldwide office locations and phone numbers, please visit Contact Us at beckman.com FLOW-2395SB03.17
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