efluor Organic Dyes 450/50 BP Fluorescence Intensity Wavelength (nm)

Transcription

1 efluor Organic Dyes

2 efluor Organic Dyes Catalog No. Description Clone Application Anti-Mouse efluor 450 Products Anti-mouse CD4 RM4-5 Flow Cytometry Anti-mouse CD Flow Cytometry Anti-mouse/human CD44 IM7 Flow Cytometry Anti-mouse CD45.1 A20 Flow Cytometry Anti-rat/mouse CD73 TY/11.8 Flow Cytometry Anti-mouse MHC class II M5/ Flow Cytometry Anti-mouse IgD 11-26c (11-26) Flow Cytometry Anti-mouse CD90.1 HIS51 Flow Cytometry Anti-mouse CD Flow Cytometry Anti-mouse CD3 17A2 Flow Cytometry Anti-Human efluor 450 Products Anti-human CD4 RPA-T4 Flow Cytometry Anti-human CD8 RPA-T8 Flow Cytometry Anti-mouse/human CD44 IM7 Flow Cytometry Anti-human CD14 61D3 Flow Cytometry Anti-human CD45RA HI100 Flow Cytometry Anti-human CD16 CB16 Flow Cytometry Anti-human CD3 OKT3 Flow Cytometry Anti-human CD19 HIB19 Flow Cytometry efluor 450 Isotype Controls and Support Products Mouse IgG2a, K Isotype Control - Flow Cytometry Mouse IgG2b Isotype Control - Flow Cytometry Rat IgG1 Isotype Control - Flow Cytometry Streptavidin - Flow Cytometry Rat IgG2a - Flow Cytometry Rat IgG2b - Flow Cytometry Mouse IgG1 - Flow Cytometry Armenian hamster Ig isotype - Flow Cytometry Fluorescence Intensity 450/50 BP Wavelength (nm)

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4 Catalog No. Description Clone Application Anti-Mouse APC-eFluor 780 Products Anti-mouse CD3 (CD3e) 17A2 Flow Cytometry Anti-mouse CD4 RM4-5 Flow Cytometry Anti-mouse CD Flow Cytometry Anti-mouse/human CD44 IM7 Flow Cytometry Anti-mouse CD Flow Cytometry Anti-rat/mouse CD90.1 HIS51 Flow Cytometry Anti-mouse CD Flow Cytometry Anti-mouse TCR beta H Flow Cytometry Anti-mouse CD117 2B8 Flow Cytometry Anti-mouse CD25 PC61.5 Flow Cytometry Anti-Human APC-eFluor 780 Products Anti-human CD3 UCHT1 Flow Cytometry Anti-human CD4 RPA-T4 Flow Cytometry Anti-human CD8 RPA-T8 Flow Cytometry Anti-human CD20 2H7 Flow Cytometry Anti-human CD27 O323 Flow Cytometry APC-eFluor 780 Isotype Controls and Support Products Armenian Hamster IgG Isotype Control - Flow Cytometry Mouse IgG1, K Isotype Control - Flow Cytometry Mouse IgG2a, K Isotype Control - Flow Cytometry Mouse IgG2b Isotype Control - Flow Cytometry Rat IgG1 Isotype Control - Flow Cytometry Rat IgG2a Isotype Control - Flow Cytometry Rat IgG2b Isotype Control - Flow Cytometry Streptavidin - Flow Cytometry Fluorescence Intensity 780/60 BP Wavelength (nm)

5 efluor Nanocrystals efluor Nanocrystals Introducing a NEW range of fluorochromes excited by the violet and UV laser for Flow Cytometry efluor 605 NC efluor 625 NC Narrow emission spectra requires minimal compensation More direct conjugates equals more choice and flexibility Fully compatible with standard organic fluorochromes High signal to noise ratios enable high quality analysis efluor Nanocrystals ( NC ) are high quantum yield nanocrystals that possess a core particle surrounded by a patented surface coating to reduce background staining and artifacts. efluor Nanocrystals are easy to use. Their unique physical and spectral properties make them excellent products for researchers performing multicolor applications. ebioscience offers a rapidly expanding portfolio of ready-to-use mouse and human reactive, and control antibody conjugates for flow cytometry. efluor nanocrystal conjugates are named for their emission wavelength. Our current offerings of efluor 605 NC and efluor 625 NC direct conjugates can be excited by either the violet or UV lasers; opening up a new range of versatile reagents for use with these lasers. for flow cytometry ebioscience New Releases October 2008 featuring CD3 (h) CD4 (h, m) CD8 (h, m) CD11b (h, m) CD11c (m) CD15 (h) CD19 (h, m) CD20 (h) CD45 (h, m) CD45RA (h) CD45R (m) GR-1 (m) HLA-DR (h) Reactivity: h=human and m=mouse Emission Spectra Comparison efluor 605 NC vs. Pacific Orange B220 efluor 605 NC B220 Pacific Orange CD3 Pacific Blue CD3 Pacific Blue efluor Nanocrystals have a narrow emission spectra compared to organic dyes. Although both efluor 605 NC and Pacific Orange will be excited by the violet laser, efluor 605 NC will be easier to compensate because of its narrow emission spectra. Comparison of efluor 605 NC and Pacific Orange staining. Mouse splenocytes were stained with Pacific Blue anti-mouse CD3 (17A2) and efluor 605 NC anti-mouse B220 (RA3-6B2) (left panel) or Pacific Orange anti-mouse B220 (RA3-6B2) (right panel). Total viable cells in the lymphocyte gate were used for analysis.

6 efluor Nanocrystals for flow cytometry for Flow Cytometry Catalog No. Description Clone Size Human Direct Conjugates , 73 efluor 605 NC anti-human CD4 OKT4 25T, 100T ,73 efluor 605 NC anti-human CD45RA HI100 25T, 100T , 73 efluor 605 NC anti-human CD11b ICRF44 25T, 100T , 73 efluor 605 NC anti-human HLA-DR LN3 25T, 100T , 73 efluor 605 NC anti-human CD19 HIB19 25T, 100T ,73 efluor 605 NC anti-human CD45 HI30 25T, 100T , 73 efluor 625 NC anti-human CD3 OKT3 25T, 100T , 73 efluor 625 NC anti-human CD8 RPA-T8 25T, 100T , 73 efluor 625 NC anti-human CD15 HI98 25T, 100T , 73 efluor 625 NC anti-human CD20 2H7 25T, 100T Mouse Direct Conjugates , 73 efluor 605 NC anti-mouse CD4 GK1.5 25T, 100T , 73 efluor 605 NC anti-mouse CD45 30-F11 25T, 100T , 73 efluor 605 NC anti-mouse CD11b M1/70 25T, 100T , 73 efluor 605 NC anti-mouse B220 RA3-6B2 25T, 100T , 73 efluor 605 NC anti-mouse CD T, 100T , 73 efluor 625 NC anti-mouse GR-1 RB6-8C5 25T, 100T , 73 efluor 625 NC anti-mouse CD19 1D3 25T, 100T , 73 efluor 625 NC anti-mouse CD11c N418 25T, 100T Mouse Isotype Controls , 94 efluor 605 NC Mouse IgG1 Isotype Control - 125µl, 500 µl , 94 efluor 605 NC Mouse IgG2a Isotype Control - 125µl, 500 µl , 94 efluor 605 NC Mouse IgG2b Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Mouse IgG1 Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Mouse IgG2a Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Mouse IgG2b Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Mouse IgM Isotype Control - 125µl, 500 µl Rat Isotype Controls , 94 efluor 605 NC Rat IgG2a Isotype Control - 125µl, 500 µl , 94 efluor 605 NC Rat IgG2b Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Rat IgG2a Isotype Control - 125µl, 500 µl , 94 efluor 625 NC Rat IgG2b Isotype Control - 125µl, 500 µl Armenian Hamster IgG Isotype Control , 94 efluor 625 NC Armenian Hamster IgG Isotype Control µl, 500 µl Streptavidin , 73 efluor 605 NC streptavidin conjugate - 25T, 100T , 73 efluor 625 NC streptavidin conjugate - 25T,100T

7 efluor Nanocrystals Introducing a NEW range of fluorochromes for Imaging and Microscopy efluor Nanocrystals ( NC ) are high quantum yield nanocrystals that possess a core particle surrounded by a patented surface coating to reduce background staining and artifacts. efluor Nanocrystals are easy to use. Their unique physical and spectral properties give them distinct advantages over organic dyes and make them excellent products for researchers performing multicolor applications. efluor nanocrystal products are named for their emission wavelength. Our rapidly growing portfolio is packaged to be economical and convenient. High photostability Narrow emission spectra compared to organic dyes Non-toxic efluor 700 NC cadmium-free InGaP nanocrystals in vivo imaging cell labeling Streptavidin efluor 605 NC for detection of biotin-labeled primary antibodies frozen or paraffin embedded tissue samples cultured cells efluor Nanocrystals for imaging & MICROSCOPY ebioscience New Releases October 2008 featuring 490 NC 525 NC 605 NC 625 NC 700 NC efluor nanocrystal products are designed for a broad range of applications including: Immunofluorescence Microscopy In vivo Imaging Flow Cytometry Tools for Discovery Immunofluorescence with efluor 605 NC efluor 700 NC for in vivo Imaging Frozen tissue section stained with Streptavidin efluor 605 NC Frozen sections of mouse lymph node were stained with either no primary antibody (left panel) or biotin anti-mouse CD3 (145-2C11) (right panel) and subsequently with streptavidin efluor 605 NC to visualize the T cell zone. FITC anti-mouse B220 (RA3-6B2) was used to identify B cell follicles. efluor Nanocrystals can be used to visualize solid tumor in vivo. Mice were injected with either PBS (left mouse) or with 100 pmol of efluor 700 NC (non-functionalized) (right mouse) into a subcutaneous tumor. efluor Nanocrystals can be used to image tissue in vivo. Mice were injected with either PBS (right mouse) or with 100 pmol of efluor 700 NC (non-functionalized) (left mouse) into the liver at a depth of 1.5 cm.

8 efluor Nanocrystals for imaging & Microscopy for Imaging and Microscopy Catalog No. Description Size efluor 605 NC streptavidin conjµgate (IF) 100 µl efluor 490 NC (Carboxyl) 200 µl efluor 525 NC (Carboxyl) 200 µl efluor 605 NC (Carboxyl) 200 µl efluor 625 NC (Carboxyl) 200 µl efluor 700 NC (Carboxyl) 250 µl efluor 490 NC (Amine) 200 µl efluor 525 NC (Amine) 200 µl efluor 605 NC (Amine) 200 µl efluor 625 NC (Amine) 200 µl efluor 700 NC (Amine) 250 µl efluor 490 NC (non-functionalized) 200 µl efluor 605 NC (non-functionalized) 200 µl efluor 625 NC (non-functionalized) 200 µl efluor 700 NC (non-functionalized) 250 µl Anti-Human (Biotin Format) CD10 (CALLA) 25 µg CD10 (CALLA) 100 µg CD15 25 µg CD µg CD20 25 µg CD µg CD84 25 µg CD µg CD µg CD µg CCR5 25 µg CCR5 100 µg Foxp3 25 µg Foxp3 100 µg Foxp3 25 µg Foxp3 100 µg CD275 (B7RP-1) 25 µg CD275 (B7RP-1) 100 µg Ig Lambda Light Chain 25 µg Ig Lambda Light Chain 100 µg Ig Kappa Light Chain 25 µg Ig Kappa Light Chain 100 µg Catalog No. Description Size Anti-Mouse (Biotin format) CD µg CD µg CD µg CD201 (EPCR) 25 µg CD201 (EPCR) 100 µg IgG 500µg GITRL 25 µg GITRL 100 µg TLR2 25 µg TLR2 100 µg CD3e 500 µg CD4 (L3T4) 500 µg CD8a 500 µg CD11b 500 µg MHC Class II (I-A/I-E) 500 µg TCRb 500 µg Foxp3 25 µg Foxp3 100 µg Isotype Controls (Biotin Format) Armenian Hamster IgG 50 µg Armenian Hamster IgG 500 µg Golden Syrian Hamster IgG 50 µg Golden Syrian Hamster IgG 500 µg Mouse IgG1 500 µg Mouse IgG2a 500 µg Mouse IgG2b 500 µg Rat IgG1 50 µg Rat IgG1 500 µg Rat IgG2a 50 µg Rat IgG2a 500 µg Rat IgG2b 50 µg Rat IgG2b 500 µg