Safety Instruction. 10 March Dr. Jost Wingender

Transcription

1 Safety Instruction According to - Ordinance on Biological Agents ( 14 Biostoffverordnung) - Ordinance on Hazardous Substances ( 14 Gefahrstoffverordnung) Applies to practical course Microbiology Bachelor Curriculum Water Science Biofilm Centre, University of Duisburg-Essen, Campus Essen Building S05 T02, Laboratories A32, A35 and A55 10 March 2017 Dr. Jost Wingender

2 Ordinance on Biological Agents Regulates safety and health protection when working with biological agents. Provides an institution with a framework for a risk assessment. Commits the employer to provide operation instructions. Demands a briefing on safety instructions for employees at least once a year and prior to commencement of work. Biohazard

3 What are biological agents? Definitions according to Ordinance on Biological Agents Biological agents are microorganisms, cell cultures and endoparasites, including their genetically modified forms, as well as agents associated with transmissible spongiform encephalopathy, which can endanger human health through infections, transmitted diseases, formation of toxins, sensitizing, or other health damaging effects. Microorganisms are all cellular and non-cellular microscopic and sub-microscopic biological units, which are capable of transferring genetical material, in particular bacteria, viruses, protozoa or fungi.

4 Classification of biological agents into risk groups Biological agents are categorized according to the risk of infection into four risk groups. Relevant biological agents used in practical course belong to risk groups 1 and 2. Risk group 1: Biological agents which are unlikely to cause illness in humans. Risk group 2: Biological agents which can cause illness in humans and present a danger to the employees; the spread of the agents in the human population is unlikely; effective preventive actions or treatment are usually possible. (Examples: Escherichia coli, Pseudomonas aeruginosa)

5 Authorization to work with pathogens (Biological agents of risk group 2) Dr. J. Wingender: authorization (according to the German Infection Protection Act) to work with pathogens belonging to risk group 1 and 2. All employees are allowed to work with pathogens belonging to risk group 1 and 2 under his supervision and do not require any other permit.

6 Working with biological agents Handling of biological agents according to the Ordinance on Biological Agents: - Isolation - Cultivation - Cell disruption - Mixing and separation - Transport - Storage - Inactivation and disposal

7 Potential transmission routes in the laboratory Potential routes of contamination or infection under laboratory conditions: - Inhalation Formation of aerosols by spilling of liquids, mixing, vortexing. - Resorption through skin Spilling of liquids, contact of hands with or mucous membranes contaminated equipment, contact with eyes, nose, mouth - Ingestion Pipetting by mouth, splashes to mouth - Inoculation Cuts, needle pricks

8 Safety rules for handling of biological agents - Keep work place clean and tidy. Remove chemicals and devices that are not permanently used. - Do not work alone, ensure visual contact to colleagues. - Keep windows and doors closed. - Wear protective clothes (lab coat, gloves, safety glasses) and sturdy shoes. - Eating, drinking, smoking, chewing gum, etc. as well as storage of food or tobacco is strictly prohibited. - Do not pipet by mouth, use mechanical transfer devices. - Keep work place clean and tidy. Remove chemicals and devices that are not permanently used. - Avoid formation of aerosols. - Exercise caution when working with the naked flame (Bunsen burner).

9 Safety rules for the handling of biological agents - After work disinfect work place (Bacillol AF; protective gloves), will be done by lab service (participants of practical course). - Before leaving the laboratory, disinfect hands (Sterillium) see hygiene plan. - After contamination of skin with biological agents, thoroughly disinfect contaminated skin area (skin disinfectant, Cutasept F). - After contact of biological agents with the eyes, rinse eyes with plenty of water (at least min); use eye shower. - Any kind of accidents need to be reported to the responsible persons (Prof. Meckenstock, Dr. Wingender). - In case of pregnancy, report immediately to Prof. Meckenstock. - Consider hygiene plan!

10 Hygiene plan

11 Wash basin with dispensers of hand disinfectant and liquid soap Hygienic hand disinfection

12 Hygiene plan

13 Hygiene plan

14 What are hazardous substances? Hazardous materials are substances or preparations have the following properties: explosive, oxidizing, extremely flammable, highly flammable, flammable, very toxic, toxic, harmful, caustic, corrosive irritant, sensitizing, carcinogenic, teratogenic, mutagenic dangerous to the environment biohazardous or materials from which hazardous or explosive substances or preparations can be produced or liberated

15 GHS - Globally Harmonized System Regulation on Classification, Labelling and Packaging of Substances and Mixtures explosive flammable oxidative Pressurized gases corrosive acute toxic Acute toxic Mutagenic Carcinogenic Reproductive toxicity respiratory sensitizing Aspiration hazard irritant, sensitizing toxic environment ally hazardous Hazardous substances Nine labels Hazard statements (H phrases) Precautionary statements (P phrases)

16 Example: disinfectant Bacillol AF

17 Intake routes of hazardous substances Possible intake routes into the human body in the laboratory: - Inhalation Dust or powder during weighing, aerosols caused by spilling of liquids, mixing, vortexing. - Resorption through skin or Spilling of liquids, contact of mucous membrane hands with contaminated equipment, hand-eye/nose/mouth contamination - Ingestion Pipeting with the mouth Splashes to the mouth - Inoculation Cuts, needle prick

18 Basic rules for handling hazardous materials Own responsibility to inform yourself prior to commencing work with hazardous materials (e.g. Ordinance on hazardous substances, operating instructions, safety data sheets, internet) Wear protective clothes (lab coat, gloves, safety glasses) and sturdy shoes Eating, drinking, smoking, etc. is prohibited No storage of foods or tobacco in the laboratory. Use hazardous materials only in quantities necessary for the experiments Do not store hazardous material in containers, which are normally used to store food! Danger of confusion! Every storage container needs to be labelled with its name and danger labels Labelling needs to be permanent with water proof pens; direct labelling with felt markers or pasting over existing labels is not permitted! Do not pipet by mouth, use mechanical transfer devices

19 Waste prevention and disposal 1. Use hazardous materials only in quantities that are necessary for the experiment 2. Waste needs to be collected or packed and labelled in the appropriate storage containers. 3. IDEXX-containers: Only for Colilert-18/Quanti-Trays waste. 4. Collect broken glass in the respective glass containers (noncontaminated/contaminated).

20 Precaution and safety devices Inform about: Escape routes (do not block escape routes) Emergency call 112 Location of emergency facilities (regularly control the functional capability, report) - first aid boxes - emergency shower - eye wash -emergency shutoff devices Fire protection equipment - fire alarm - fire extinguishers - official fire regulations

21 Behavior in dangerous situations Stay calm Protecting persons prior to equipment protection Protecting people prior to fire protection First inform others (fire alarm), then fight fire protect your own safety Provide first aid

22 Behavior in dangerous situations Accidents/irregularities - immediately inform others (Prof. Meckenstock etc.), project leader, warn colleagues. All injuries/accidents have to be recorded in the accident book ( Verbandsbuch ); records have to be stored at least 10 years) Wipe up spilled suspensions containing biological material, using tissue paper (autoclave) Desinfect contaminated equipment and surfaces with Bacillol AF (15 min incubation) After skin contact with biological agents desinfect with skin disinfectant. Bring injured persons out of the danger zone and provide fresh air. Extinguish burning clothes. If necessary, use emergency shower. If contaminated, clothes have to be removed before; wash with soap and water.

23 Behavior in dangerous situations Stop bleedings and apply a bandage (use examination gloves) If eyes are contaminated with biological material, wash out the eyes with huge amount of water (at least 10 min); use the eye wash station After intensive contact (e.g. ingestion), consult doctor Do not leave injured person alone. Collect information for the doctor. (vomit, chemicals and other details, e.g. safety data sheets) Autoclave contaminated clothes.

24 Emergency telephone numbers 3333 In-house emergency call connection with switchboard (inform someone from the switchboard at the latest after all rescue measures are done) 112 Fire Department, emergency doctor, rescue service - from all telephones inside the university at each time possible - free emergency call from public telephones - Mobile phone Meeting place: lawn in front of building S05