Tube Sequencing Services. Whatever Sample Type You Want To Send In Tubes.
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1 Tube Sequencing Services Whatever Sample Type You Want To Send In Tubes.
2 Tube Sequencing Services. High quality sequencing for every sample type Mix2Seq Mix2Seq is the fastest sequencing of premixed samples available today. It is offered as overnight and non-overnight sequencing service. Order a Mix2Seq Kit and receive 96 single reactions; no further online orders are required. TubeSeq Service This is the most convenient sequencing service for plasmids, PCR products and large constructs. Up to 8 reactions per sample can be ordered and re-ordered. For complex sequences a Power Read Upgrade is offered. DNA preparation, purification, sample storage and more additional services are available. TubeSeq Labels TubeSeq Labels are the prepaid barcode labels of our TubeSeq Service. They offer access to fast DNA sequencing with our express logistics and automated workflows. Ready2Load The Ready2Load service allows you to submit your pre-cycled samples in tubes to get them analysed with our ABI sequencers. Additional benefits to our tube sequencing services: Convenient ordering and tracking online Free sample pick up from your DropBox location Labels and envelops are available for sample shipment Secure online archive of sequence data for 100 days Useful sequencing accessories are available Order settings can be personalised for each order Easy-to-use primer design tool with direct ordering
3 Mix2Seq. Ultimate innovation and highest convenience Ultimate innovation Buy only one Mix2Seq Kit to pay for 96 reactions. One Mix2Seq Kit & its data can be shared with others. Mix2Seq Kits can be activated or transferred online. Highest convenience Every Mix2Seq Kit inlcudes 96 barcoded tubes and lids. Just provide the tubes - no online ordering necessary. Under My Kits & Labels you can track all kits & results. Excellent quality & service Get your samples sequenced either overnight or next day. Obtain reading lengths of up to 1,100 high quality bases. All sequencing data are available as traces and fasta files. Mix2Seq Kit - OVERNIGHT Mix2Seq Kit Three little steps to optimum sequencing results Order Mix2Seq Kit Buy your Mix2Seq Kit with 96 pre-labelled tubes and lids. Prepare template Send us 15µl purified DNA in either of the concentrations stated in your Mix2Seq box. Add 2µl of your primer with a concentration of 10pmol/µl. Ensure a total volume of 17µl. Download results Securely download your Mix2Seq results in your personal online account under My Kits & Labels.
4 TubeSeq Service & TubeSeq Labels. Combined power of automation & expertise The TubeSeq Service combines long term expertise in automated, barcode driven sequencing processes with the handling of samples, which require bespoke solutions. All benefits of the TubeSeq Service at a glance: High quality read lengths of up to 1,100 bases Additional services like DNA purification available Free one time repeat, in case of technical failure Advanced analysis options are available for free Different sequencing result files can be selected Storage of DNA and ordered primers for 4 weeks Primer storage for one year on request Easy re-order options from stored DNA samples The highlights of the TubeSeq Service include: Order and re-order up to 8 reactions per sample Sequencing reads can be paid with our TubeSeq Labels All type of samples, incl. large constructs, are accepted Power Read Upgrade* for more complex sequences Results of standard DNA reads are available next day *In case your samples are >30kbp /or your samples contain difficultto-read stretches, select the Power Read Upgrade. We will perform special cycling conditions or chemistry to achieve best results.
5 Ready2Load. For pre-cycled samples ready to load Ready2Load Plate is our service which allows you to conveniently get your pre-cycled samples in tubes analysed on our ABI sequencers. What you need to do You need to carry out the sequencing reaction according to the BigDye Terminator v3.1 cycle sequencing kit protocol from ABI. What we do for you: Perform the dye terminator removal Load the purified samples on our ABI sequencers Sequence the samples under standard parameters Upload the sequencing results to your online account
6 Selection Guide. Selection Guide. Services & Options Mix2Seq TubeSeq Ready2Load Payment options Barcodes / Kits Prepaid Non-prepaid Tube Labels TubeSeq Labels (Prepaid) Kit with 96 matrix tubes & lids Sample types Plasmids PCR products BAC / PAC / Cosmid DNA Sample specification Premixed (DNA + primer) Pre-cycled samples DNA preparation Additional services PCR product purification Concentration adjustment Power Read for difficult samples Free pick-up via DropBoxes Shipping options Postage using sample bags Courier using DHL Shipping Kit Tube bags & boxes for shipping Delivery time Next day upon sample receipt Overnight - next day until noon IUPAC bases in the sequence N for unclear bases Sequencing data Sequence clipping (Q20, 30, 40) Selectable result file types Secure download of data Additional services for free Online archive of data Repetition in case of technical failure Storage of sample DNA for 4 weeks Storage of ordered primers for 4 weeks Standard primers on stock
7 Selection Guide. Additional Services. Sequencing primers and sample shipment Sequencing primers Benefit from our in-house oligo production: More than 80 verified standard primers for free Synthesis of ordered sequencing primers within 24 hours Storage of ordered primers for 4 weeks Primer storage for one year, on request Free tube labels for your accompanying primers Free sample pick-up service Hundreds of Eurofins Genomics DropBoxes are installed in Europe. Use a DropBox nearby for free sample pick-up. Sample shipment Conveniently order your shipping material of choice in our online shop for sending your sequencing samples where no DropBox is available: DHL labels and envelops for courier shipment Padded envelops and address labels for mail dispatch Plate sample boxes for secure packaging
8 Feel Free To Contact Us. Toll free phone numbers Austria Belgium Denmark Finland France Ireland Italy Luxemburg Netherlands Norway Sweden Switzerland UK Phone: Official business hours: Monday to Friday 8 a.m. 6 p.m. CET (excluding public holidays in Germany) Eurofins Genomics Anzinger Str. 7a Ebersberg eurofinsgenomics.com 2017_0412/05_2017/2.000
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